Status
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Gene information
General information
TC015821 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8932304..8941584
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015821
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05903

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    gut remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015821 RA
      MCKSPKNST NSYQIKPEK IDYENLSTN CTVVGSNNL 
NLVKKPQIV TNMSPAYSN PSPGYPPSP YSSPASLKS 
PSPNLKPPT PQPNFPVMQ IINSHSLLA RPIQTNQSQ 
NIIKLKPHL NILPKPSAS PQPSPKPSV SPQIVIPTN 
QPTIMPTAQ PVLLNQMPL LTTPGVQFI LRPQTAKIP 
AAAPQGLIL QPSGQPLLQ IPRSQPMVR VLTNGVQLA 
PSTTTTAYV TTQMTNPQP PNNQVITSQ PHRSEHSVQ 
PNTTSQMAV GPRLAVPQV QQQQHKQPQ PQQQQQPQP 
PQQQQQPQQ QVVAPPPTL QQHSHSITK KAHLIEAQL 
NLDQSGAVK PDIHTPFRD KKDACIRLI RYHCMDQPV 
LSQKDLDKA DEIFELTAE HFIAKYAKM VDKYKYLLM 
KESMRPVQT SELMMLDRM FLSEERQSL LRLRQEAEE 
AAQIIDLPV LPEPSPPQP PEDYDEWAC IQRELGCLP 
HEEPPPAAP RPPHSTAAP VVANAHAHL QPPKRTASS 
DSRLETLKR FRVDKHNKK TSTESVHNN SVLNNNNSS 
VQSVSSGIN THHGYEQQG QNDSYENEV ENNSIDEQV 
QSAIDSILN LQQNTALDL DSILS
    CDS sequence
    • >TC015821 RA
      atgtgcaaa tcaccgaaa aattcaaca aacagctac 
caaatcaag ccggaaaag atcgactat gagaacctc 
agtaccaac tgcactgtc gtcggctcc aacaatctc 
aacttggtg aaaaaaccc caaatcgta accaacatg 
tctccagcc tactcgaac cccagtcca ggctaccca 
ccctcacca tactcgtcc ccagctagt ctcaaatca 
ccttcaccc aacctcaaa cctccaact cctcaacca 
aacttccca gtaatgcaa atcatcaac agccactcc 
cttctggca cgcccaatc caaaccaac caatcacag 
aacattatt aaactcaag ccacacctt aatatccta 
cccaaaccc agcgccagc ccgcaacca agccctaaa 
ccgagcgtc agcccacag atcgtcatt ccgaccaat 
cagccaacg ataatgcca actgctcaa ccggtgctt 
ctcaaccaa atgcctcta ctgaccaca ccaggggtc 
cagttcata ttgcgcccc cagacggcc aaaatcccg 
gcggcggcc ccccaagga ctgatcctg caaccgagc 
ggacagccc ctcctacaa atacccaga tctcagccc 
atggttcgt gttttaacc aatggcgta caacttgcc 
ccctctacc accaccaca gcctacgtc acgacgcaa 
atgaccaat ccgcagccg ccaaacaat caagtgata 
acatcgcaa ccgcatcgg tcagaacac tcggtacaa 
ccgaacacg actagtcaa atggccgta ggtccacgt 
ctcgctgta cctcaagta caacaacag caacacaaa 
caacctcaa ccacaacaa caacaacaa ccgcaacca 
cctcaacaa caacaacaa ccacaacaa caagtggtg 
gcgccacct ccaacgcta caacagcac agtcattca 
attacgaaa aaagcacac ttaattgaa gcacagtta 
aatctggac caatcaggt gccgtcaaa ccggacatc 
catacgcca tttcgcgat aagaaggac gcatgtatt 
cgcctgata aggtatcac tgtatggat cagcccgtc 
ttatcgcag aaggatctg gacaaggcc gatgagatt 
ttcgaacta accgccgaa catttcatc gccaagtat 
gcgaaaatg gtcgacaag tacaaatat ttactaatg 
aaggagagc atgcgaccg gtgcaaaca tccgaacta 
atgatgctc gaccgaatg ttcctcagc gaggagcgg 
cagtcgttg ctgcgtctg cggcaggag gccgaagag 
gcggcgcaa ataatcgac ttgcccgtt ctgcccgag 
ccttcgccc ccgcagccc cccgaggac tacgacgaa 
tgggcgtgc attcaacgc gagctcggt tgcctcccg 
cacgaagag ccgccgccg gccgcccca cggccccca 
cattcgacg gcggcgccg gtcgtcgcc aacgcgcat 
gcgcacctc cagccaccc aaacgcacg gcgagcagc 
gattcgcgc ctcgagact ttgaaaagg tttcgcgtc 
gacaaacac aacaagaaa accagtaca gaaagtgta 
cataacaat agtgtttta aataataac aatagtagt 
gtacaaagt gtcagtagt gggataaat actcatcac 
ggttacgaa cagcaaggc cagaacgac tcgtacgaa 
aacgaagtc gaaaataat agtatcgat gaacaggtc 
cagagtgcc atcgattcg attttgaat ctacaacag 
aatacggcc ctggacctt gatagtatt ctttcatga
    mRNA sequence
    • >TC015821 RA
      gcgtagtag tggaccccg tttttgcca atgaagtga 
aaattgttt gaggttttg cggcgattt ctgactaaa 
atcaggcaa gaatcggta aaatcaaca ttgtcctca 
acgaaaatt cggccgaaa ttgacccaa aacctggct 
ccatggaag aaaccccct cgaaataat gccttggct 
tgcggagtt ccctcttgt tgtctacaa cgataaaat 
ggccctatt cgagtttgg ctaaaagtg ataagtgct 
tctattaag actcgcaac tcggactcg cataaacat 
tgcaacaaa atgagttag tttaagttc cgcgacatt 
ccggtgtaa aataattcg aaaaaaatc caatcaaaa 
atgtcgtac ttaaagata ctgtgaatc gtaccgggg 
gtttagtgt tctcgtttt tcgcaacgt gtagaaaaa 
tgtctcaaa cgccccaaa aactcggtc aaaaaatgt 
gcaaatcac cgaaaaatt caacaaaca gctaccaaa 
tcaagccgg aaaagatcg actatgaga acctcagta 
ccaactgca ctgtcgtcg gctccaaca atctcaact 
tggtgaaaa aaccccaaa tcgtaacca acatgtctc 
cagcctact cgaacccca gtccaggct acccaccct 
caccatact cgtccccag ctagtctca aatcacctt 
cacccaacc tcaaacctc caactcctc aaccaaact 
tcccagtaa tgcaaatca tcaacagcc actcccttc 
tggcacgcc caatccaaa ccaaccaat cacagaaca 
ttattaaac tcaagccac accttaata tcctaccca 
aacccagcg ccagcccgc aaccaagcc ctaaaccga 
gcgtcagcc cacagatcg tcattccga ccaatcagc 
caacgataa tgccaactg ctcaaccgg tgcttctca 
accaaatgc ctctactga ccacaccag gggtccagt 
tcatattgc gcccccaga cggccaaaa tcccggcgg 
cggcccccc aaggactga tcctgcaac cgagcggac 
agcccctcc tacaaatac ccagatctc agcccatgg 
ttcgtgttt taaccaatg gcgtacaac ttgccccct 
ctaccacca ccacagcct acgtcacga cgcaaatga 
ccaatccgc agccgccaa acaatcaag tgataacat 
cgcaaccgc atcggtcag aacactcgg tacaaccga 
acacgacta gtcaaatgg ccgtaggtc cacgtctcg 
ctgtacctc aagtacaac aacagcaac acaaacaac 
ctcaaccac aacaacaac aacaaccgc aaccacctc 
aacaacaac aacaaccac aacaacaag tggtggcgc 
cacctccaa cgctacaac agcacagtc attcaatta 
cgaaaaaag cacacttaa ttgaagcac agttaaatc 
tggaccaat caggtgccg tcaaaccgg acatccata 
cgccatttc gcgataaga aggacgcat gtattcgcc 
tgataaggt atcactgta tggatcagc ccgtcttat 
cgcagaagg atctggaca aggccgatg agattttcg 
aactaaccg ccgaacatt tcatcgcca agtatgcga 
aaatggtcg acaagtaca aatatttac taatgaagg 
agagcatgc gaccggtgc aaacatccg aactaatga 
tgctcgacc gaatgttcc tcagcgagg agcggcagt 
cgttgctgc gtctgcggc aggaggccg aagaggcgg 
cgcaaataa tcgacttgc ccgttctgc ccgagcctt 
cgcccccgc agccccccg aggactacg acgaatggg 
cgtgcattc aacgcgagc tcggttgcc tcccgcacg 
aagagccgc cgccggccg ccccacggc ccccacatt 
cgacggcgg cgccggtcg tcgccaacg cgcatgcgc 
acctccagc cacccaaac gcacggcga gcagcgatt 
cgcgcctcg agactttga aaaggtttc gcgtcgaca 
aacacaaca agaaaacca gtacagaaa gtgtacata 
acaatagtg ttttaaata ataacaata gtagtgtac 
aaagtgtca gtagtggga taaatactc atcacggtt 
acgaacagc aaggccaga acgactcgt acgaaaacg 
aagtcgaaa ataatagta tcgatgaac aggtccaga 
gtgccatcg attcgattt tgaatctac aacagaata 
cggccctgg accttgata gtattcttt catgacggt 
atgtctagt ggatatggc caggctgca ctgtaatac 
acacacgtt ttctctcca ttgtgatca ttacttttt 
aaaagtcat ttattttta cagatttta aaacgaagc 
gataaatta tatacacag ctcaatacg gcatctaga 
gcccgcgga cgtcacgtg gcgcattat tggttacca 
tggttacca ctgaaaaaa gaaacaaaa cataggcta 
aattacaaa atttgtgca aacaccctg tatgacatt 
tttcccatt ttttggttg attagcgag ggtcatgtg 
acgtccctg ggcccgatc gttgtgacg gtgcgattt 
ttcacagtg taccacaat tgtgtcata tcactgttg 
caataatat tgttttgtt ttaattgag gcagctatg 
gaacccaga ttagatgtc gagcaataa acgttttaa 
ctttgattt taattattc ctcaaatgt ttccacatg 
attgtagca cactgtaaa aacttggat attttttat 
tatattgta aataattta atttatgtg tacttagtt 
tatttttta ttttctaag gttttatta ttattaaga 
ctgacttta agaaatttt ggttcctaa acggaagaa 
gtatttcaa ataaaattg aattgtttc a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY