General information | |||
TC015821 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 8932304..8941584 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC015821 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05903 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
gut remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015821 RA
MCKSPKNST NSYQIKPEK IDYENLSTN CTVVGSNNL NLVKKPQIV TNMSPAYSN PSPGYPPSP YSSPASLKS PSPNLKPPT PQPNFPVMQ IINSHSLLA RPIQTNQSQ NIIKLKPHL NILPKPSAS PQPSPKPSV SPQIVIPTN QPTIMPTAQ PVLLNQMPL LTTPGVQFI LRPQTAKIP AAAPQGLIL QPSGQPLLQ IPRSQPMVR VLTNGVQLA PSTTTTAYV TTQMTNPQP PNNQVITSQ PHRSEHSVQ PNTTSQMAV GPRLAVPQV QQQQHKQPQ PQQQQQPQP PQQQQQPQQ QVVAPPPTL QQHSHSITK KAHLIEAQL NLDQSGAVK PDIHTPFRD KKDACIRLI RYHCMDQPV LSQKDLDKA DEIFELTAE HFIAKYAKM VDKYKYLLM KESMRPVQT SELMMLDRM FLSEERQSL LRLRQEAEE AAQIIDLPV LPEPSPPQP PEDYDEWAC IQRELGCLP HEEPPPAAP RPPHSTAAP VVANAHAHL QPPKRTASS DSRLETLKR FRVDKHNKK TSTESVHNN SVLNNNNSS VQSVSSGIN THHGYEQQG QNDSYENEV ENNSIDEQV QSAIDSILN LQQNTALDL DSILS
- >TC015821 RA
atgtgcaaa tcaccgaaa aattcaaca aacagctac caaatcaag ccggaaaag atcgactat gagaacctc agtaccaac tgcactgtc gtcggctcc aacaatctc aacttggtg aaaaaaccc caaatcgta accaacatg tctccagcc tactcgaac cccagtcca ggctaccca ccctcacca tactcgtcc ccagctagt ctcaaatca ccttcaccc aacctcaaa cctccaact cctcaacca aacttccca gtaatgcaa atcatcaac agccactcc cttctggca cgcccaatc caaaccaac caatcacag aacattatt aaactcaag ccacacctt aatatccta cccaaaccc agcgccagc ccgcaacca agccctaaa ccgagcgtc agcccacag atcgtcatt ccgaccaat cagccaacg ataatgcca actgctcaa ccggtgctt ctcaaccaa atgcctcta ctgaccaca ccaggggtc cagttcata ttgcgcccc cagacggcc aaaatcccg gcggcggcc ccccaagga ctgatcctg caaccgagc ggacagccc ctcctacaa atacccaga tctcagccc atggttcgt gttttaacc aatggcgta caacttgcc ccctctacc accaccaca gcctacgtc acgacgcaa atgaccaat ccgcagccg ccaaacaat caagtgata acatcgcaa ccgcatcgg tcagaacac tcggtacaa ccgaacacg actagtcaa atggccgta ggtccacgt ctcgctgta cctcaagta caacaacag caacacaaa caacctcaa ccacaacaa caacaacaa ccgcaacca cctcaacaa caacaacaa ccacaacaa caagtggtg gcgccacct ccaacgcta caacagcac agtcattca attacgaaa aaagcacac ttaattgaa gcacagtta aatctggac caatcaggt gccgtcaaa ccggacatc catacgcca tttcgcgat aagaaggac gcatgtatt cgcctgata aggtatcac tgtatggat cagcccgtc ttatcgcag aaggatctg gacaaggcc gatgagatt ttcgaacta accgccgaa catttcatc gccaagtat gcgaaaatg gtcgacaag tacaaatat ttactaatg aaggagagc atgcgaccg gtgcaaaca tccgaacta atgatgctc gaccgaatg ttcctcagc gaggagcgg cagtcgttg ctgcgtctg cggcaggag gccgaagag gcggcgcaa ataatcgac ttgcccgtt ctgcccgag ccttcgccc ccgcagccc cccgaggac tacgacgaa tgggcgtgc attcaacgc gagctcggt tgcctcccg cacgaagag ccgccgccg gccgcccca cggccccca cattcgacg gcggcgccg gtcgtcgcc aacgcgcat gcgcacctc cagccaccc aaacgcacg gcgagcagc gattcgcgc ctcgagact ttgaaaagg tttcgcgtc gacaaacac aacaagaaa accagtaca gaaagtgta cataacaat agtgtttta aataataac aatagtagt gtacaaagt gtcagtagt gggataaat actcatcac ggttacgaa cagcaaggc cagaacgac tcgtacgaa aacgaagtc gaaaataat agtatcgat gaacaggtc cagagtgcc atcgattcg attttgaat ctacaacag aatacggcc ctggacctt gatagtatt ctttcatga
- >TC015821 RA
gcgtagtag tggaccccg tttttgcca atgaagtga aaattgttt gaggttttg cggcgattt ctgactaaa atcaggcaa gaatcggta aaatcaaca ttgtcctca acgaaaatt cggccgaaa ttgacccaa aacctggct ccatggaag aaaccccct cgaaataat gccttggct tgcggagtt ccctcttgt tgtctacaa cgataaaat ggccctatt cgagtttgg ctaaaagtg ataagtgct tctattaag actcgcaac tcggactcg cataaacat tgcaacaaa atgagttag tttaagttc cgcgacatt ccggtgtaa aataattcg aaaaaaatc caatcaaaa atgtcgtac ttaaagata ctgtgaatc gtaccgggg gtttagtgt tctcgtttt tcgcaacgt gtagaaaaa tgtctcaaa cgccccaaa aactcggtc aaaaaatgt gcaaatcac cgaaaaatt caacaaaca gctaccaaa tcaagccgg aaaagatcg actatgaga acctcagta ccaactgca ctgtcgtcg gctccaaca atctcaact tggtgaaaa aaccccaaa tcgtaacca acatgtctc cagcctact cgaacccca gtccaggct acccaccct caccatact cgtccccag ctagtctca aatcacctt cacccaacc tcaaacctc caactcctc aaccaaact tcccagtaa tgcaaatca tcaacagcc actcccttc tggcacgcc caatccaaa ccaaccaat cacagaaca ttattaaac tcaagccac accttaata tcctaccca aacccagcg ccagcccgc aaccaagcc ctaaaccga gcgtcagcc cacagatcg tcattccga ccaatcagc caacgataa tgccaactg ctcaaccgg tgcttctca accaaatgc ctctactga ccacaccag gggtccagt tcatattgc gcccccaga cggccaaaa tcccggcgg cggcccccc aaggactga tcctgcaac cgagcggac agcccctcc tacaaatac ccagatctc agcccatgg ttcgtgttt taaccaatg gcgtacaac ttgccccct ctaccacca ccacagcct acgtcacga cgcaaatga ccaatccgc agccgccaa acaatcaag tgataacat cgcaaccgc atcggtcag aacactcgg tacaaccga acacgacta gtcaaatgg ccgtaggtc cacgtctcg ctgtacctc aagtacaac aacagcaac acaaacaac ctcaaccac aacaacaac aacaaccgc aaccacctc aacaacaac aacaaccac aacaacaag tggtggcgc cacctccaa cgctacaac agcacagtc attcaatta cgaaaaaag cacacttaa ttgaagcac agttaaatc tggaccaat caggtgccg tcaaaccgg acatccata cgccatttc gcgataaga aggacgcat gtattcgcc tgataaggt atcactgta tggatcagc ccgtcttat cgcagaagg atctggaca aggccgatg agattttcg aactaaccg ccgaacatt tcatcgcca agtatgcga aaatggtcg acaagtaca aatatttac taatgaagg agagcatgc gaccggtgc aaacatccg aactaatga tgctcgacc gaatgttcc tcagcgagg agcggcagt cgttgctgc gtctgcggc aggaggccg aagaggcgg cgcaaataa tcgacttgc ccgttctgc ccgagcctt cgcccccgc agccccccg aggactacg acgaatggg cgtgcattc aacgcgagc tcggttgcc tcccgcacg aagagccgc cgccggccg ccccacggc ccccacatt cgacggcgg cgccggtcg tcgccaacg cgcatgcgc acctccagc cacccaaac gcacggcga gcagcgatt cgcgcctcg agactttga aaaggtttc gcgtcgaca aacacaaca agaaaacca gtacagaaa gtgtacata acaatagtg ttttaaata ataacaata gtagtgtac aaagtgtca gtagtggga taaatactc atcacggtt acgaacagc aaggccaga acgactcgt acgaaaacg aagtcgaaa ataatagta tcgatgaac aggtccaga gtgccatcg attcgattt tgaatctac aacagaata cggccctgg accttgata gtattcttt catgacggt atgtctagt ggatatggc caggctgca ctgtaatac acacacgtt ttctctcca ttgtgatca ttacttttt aaaagtcat ttattttta cagatttta aaacgaagc gataaatta tatacacag ctcaatacg gcatctaga gcccgcgga cgtcacgtg gcgcattat tggttacca tggttacca ctgaaaaaa gaaacaaaa cataggcta aattacaaa atttgtgca aacaccctg tatgacatt tttcccatt ttttggttg attagcgag ggtcatgtg acgtccctg ggcccgatc gttgtgacg gtgcgattt ttcacagtg taccacaat tgtgtcata tcactgttg caataatat tgttttgtt ttaattgag gcagctatg gaacccaga ttagatgtc gagcaataa acgttttaa ctttgattt taattattc ctcaaatgt ttccacatg attgtagca cactgtaaa aacttggat attttttat tatattgta aataattta atttatgtg tacttagtt tatttttta ttttctaag gttttatta ttattaaga ctgacttta agaaatttt ggttcctaa acggaagaa gtatttcaa ataaaattg aattgtttc a
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
