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Gene information
General information
TC015812 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8848327..8849777
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015812
    iBeetle screen iB_05901

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    vitellarium not present - Number of animals showing the phenotype: 2

    vitellarium mostly not present - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015812 RA
      MAEKLGLGS EVRQLKIGQ SFTNPKSSA FHSIKYDFK 
      PASVDTNKI ATVDVGNNN QVTVTVPHL DGAGVPQTV 
      FKGSQRPYQ KECVLIIDR ATGEITLEK LTCNIQVKK 
      TRSESAKQP LPQATDRSG TTSMRSQTP PIGQRISHK 
      TKVTSGSRR PDRPITHLV PKHSPLHAS PSYPSPKSH 
      ANETHQSTL ASLPMIGID DFDAPPPPS STDKSPSMF 
      PLSDHETAG VGEMSDSSS SSSDSDSDS DNPDLTAVN 
      KLSKTNGHS NGTSAATKI STHLLNEDL CLSESGSDS 
      D
    CDS sequence
    • >TC015812 RA
      atggcagaa aaactgggt ttaggtagc gaagttcgg 
      cagctgaag ataggccag agtttcaca aatccgaaa 
      tcatcggcg ttccattcg attaaatac gatttcaag 
      ccagcctcg gtcgacacc aacaaaata gccaccgta 
      gatgtcggc aacaacaac caagtcaca gttaccgta 
      cctcacttg gatggtgca ggggtgccc caaacagta 
      ttcaaaggc tcgcagcgg ccttaccaa aaagaatgc 
      gtgctgata atcgaccgc gccaccggc gaaataacc 
      ctagagaaa ctaacgtgt aatattcaa gtgaaaaag 
      acgcgaagt gagtcggcc aagcagccg ctgccccag 
      gccaccgac cgctcgggc acgacgtcc atgcgctcg 
      cagacgccc cccatcggc cagcgcatc tcgcacaag 
      acgaaagtg accagcggg agccgccga ccggaccgc 
      cccatcacc catctggtg ccaaagcac tcgcccctg 
      cacgcgagc cccagttac ccctcgccc aagagccac 
      gccaacgag acgcaccag agcacgctc gcgagtctg 
      ccaatgatc ggcatcgac gacttcgac gcgcccccg 
      ccccccagc tccaccgac aaatcgccg tcgatgttc 
      cccctctcc gaccacgag accgctgga gtgggggaa 
      atgagcgac tcgtcgtcg tcgagcagc gacagcgac 
      tcggattcg gataatcca gaccttacc gccgttaac 
      aaattatcc aaaaccaat gggcacagc aatgggacc 
      tcagcagcc accaaaata tccacacat ctcttaaac 
      gaagacttg tgcttgtca gagtcgggc tcagactcg 
      gactaa
    mRNA sequence
    • >TC015812 RA
      tggaattaa tgaaataac cgcttcaac aaacagtgc 
      attgtgtga aacgcgcaa aattatgtg tttttcata 
      attgtggcg tttaaaagg tgcactttg tgggttttt 
      agctcgtcc gcaaaccac ctgtcaaaa atgtcaaat 
      ccgtcaaaa agtaggtca atattttac ttttcaata 
      cgtatgctc atatgttag ggaataatt ggtcaaatt 
      tggtaaatc cgtccgtgt ttctgacgt tccaccatt 
      ctaaccaaa cttgcaatg gattaaaat cgcaaaaat 
      ggcagaaaa actgggttt aggtagcga agttcggca 
      gctgaagat aggccagag tttcacaaa tccgaaatc 
      atcggcgtt ccattcgat taaatacga tttcaagcc 
      agcctcggt cgacaccaa caaaatagc caccgtaga 
      tgtcggcaa caacaacca agtcacagt taccgtacc 
      tcacttgga tggtgcagg ggtgcccca aacagtatt 
      caaaggctc gcagcggcc ttaccaaaa agaatgcgt 
      gctgataat cgaccgcgc caccggcga aataaccct 
      agagaaact aacgtgtaa tattcaagt gaaaaagac 
      gcgaagtga gtcggccaa gcagccgct gccccaggc 
      caccgaccg ctcgggcac gacgtccat gcgctcgca 
      gacgccccc catcggcca gcgcatctc gcacaagac 
      gaaagtgac cagcgggag ccgccgacc ggaccgccc 
      catcaccca tctggtgcc aaagcactc gcccctgca 
      cgcgagccc cagttaccc ctcgcccaa gagccacgc 
      caacgagac gcaccagag cacgctcgc gagtctgcc 
      aatgatcgg catcgacga cttcgacgc gcccccgcc 
      ccccagctc caccgacaa atcgccgtc gatgttccc 
      cctctccga ccacgagac cgctggagt gggggaaat 
      gagcgactc gtcgtcgtc gagcagcga cagcgactc 
      ggattcgga taatccaga ccttaccgc cgttaacaa 
      attatccaa aaccaatgg gcacagcaa tgggacctc 
      agcagccac caaaatatc cacacatct cttaaacga 
      agacttgtg cttgtcaga gtcgggctc agactcgga 
      ctaaccggc gagttaagg taatcgata attaaaata 
      tttgttaca gaatatata ttatgtatg ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY