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Gene information
General information
TC015809 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8798423..8812797
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015809
    iBeetle screen iB_05900

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015809 RA
      MDPISASCF SLHAEENAR AIIEEKKQD EKFHEIIVS 
      CRSLSHYDG RTIKTLLPQ IFATVLSAS FHIVVGISL 
      AFSGILLPQ LDDEKFPIT PQQRPWIAS VIVLAVPLG 
      AVAGGFIMD AIGRLNTVK LAAIPGVFG WTLIAMATN 
      FHMLIIGRL LTGLASALG TSPAIVYLT EIARADMRG 
      SLISFAPAY ASLGMVLTF LKGWFFSWR VVAWTCLGY 
      TVIPCVLLM FIPESPAWL VSKGKIEQA SKSLAWINK 
      YQPQPENKP QTLAEMQLA QLQKEHQKK LDEAALHGR 
      GAAYKARAF LKPTGYKPL LILIGLFLC QQFSGIYIT 
      LFHSVEFFQ AVGSPVNAY LASVLISTV RLFMSVLDT 
      YLLRTFSRR PLIMLSGFT MATCMFFSG LFTKWITEG 
      TTDATWVPV AFLLLYVIA SMLGLLPIP WTMTAELFP 
      IEIRGVAHS IAYSSANIL MFGAVQCYE VLMTSLKGA 
      AGVQFFFAV ICIIAMVYT FVFVPETHR KKLTEIEEY 
      FNHNMIYLG QKPQKGGKK DKSRDKEQT EKLMKV
    CDS sequence
    • >TC015809 RA
      atggatcca atcagtgcc agttgcttc agtttacat 
      gctgaagaa aacgctcga gcaataata gaggagaaa 
      aaacaagat gaaaaattt cacgaaatc atcgtcagt 
      tgtcgatct ctcagccac tacgatggg cgaacaatt 
      aaaacattg ctacctcaa atttttgca acagtattg 
      tccgcttca tttcacata gtggtcgga atatctttg 
      gctttttcc ggcatactt ttaccccaa cttgacgac 
      gaaaaattc ccgattacg ccacaacaa aggccatgg 
      attgcaagt gtgatagtt ttggctgtg ccacttggc 
      gccgtcgct ggtggcttt ataatggac gctatagga 
      agactgaat actgttaag ttggcagca atacctggg 
      gttttcgga tggacgtta atcgccatg gctaccaac 
      ttccacatg ctgataatc gggcggctt ttaacagga 
      ctggcgtca gctttgggg accagtcct gccatcgtc 
      tacctgacg gaaattgca agggctgac atgagaggc 
      tcgttgatt tccttcgcc cccgcatat gcctcctta 
      ggtatggta cttacattt ttgaaaggt tggttcttc 
      agttggcgc gtcgttgca tggacttgt ctcggctac 
      actgtaatt ccttgtgtt ctactaatg ttcatacct 
      gaaagtccc gcttggttg gtatcgaaa ggaaaaatc 
      gaacaagcg agtaaatca ctggcttgg atcaacaag 
      taccaacca caacccgaa aataaacca caaacttta 
      gccgaaatg cagttggca caactgcaa aaagaacac 
      caaaagaaa ctagacgag gcagccctg catggccgg 
      ggggcggcc tacaaggcc cgcgcgttc ctcaaaccc 
      acgggctac aagccgttg ctgatcctg attggttta 
      ttcttatgt caacagttt tcggggatt tacatcacc 
      ctgttccac tcggtcgag tttttccag gcggttggt 
      agtcctgtg aacgcgtac ttggcctct gtcctcatc 
      agcaccgtg cgtcttttt atgtcagtg cttgacact 
      tacctcctg cgcacgttc agccggcgc ccccttatc 
      atgttgagt ggcttcacc atggccacg tgcatgttt 
      ttttcgggg cttttcacc aagtggatc accgagggg 
      accactgac gccacgtgg gtccccgtg gcctttctt 
      ttactttac gtcatagcg tcaatgttg gggctttta 
      cccattccg tggacgatg acagccgaa ctgttcccg 
      attgagata aggggcgtg gctcacagt atcgcatac 
      agtagtgcc aacatactc atgtttggg gccgtccaa 
      tgctacgaa gtgctaatg acctcgctt aagggggct 
      gctggagtg cagtttttc ttcgctgtg atttgcatt 
      attgcaatg gtttatacg tttgtgttc gttcccgaa 
      acgcaccgg aagaagttg acggaaatt gaggagtat 
      ttcaaccac aatatgatc tatttaggg cagaagccc 
      cagaaaggg gggaagaag gacaagagt agggacaag 
      gaacaaaca gagaagttg atgaaagtt tga
    mRNA sequence
    • >TC015809 RA
      agccgttta tctcgcttc tgattggct aagtcgccc 
      atttttggg gtggtgggg tgctatcga ccgcaagct 
      cccatctcg acgcatgtg ggctaccag ctgatggta 
      tgaaatagc ggaaaactc gattattag cggcgtgat 
      cgcgataca gagtggaag ggttttcat gtgagtggt 
      taaagcagc ggtgtgact ttgtccgtc taactgtac 
      ctgaaaaat catggatcc aatcagtgc cagttgctt 
      cagtttaca tgctgaaga aaacgctcg agcaataat 
      agaggagaa aaaacaaga tgaaaaatt tcacgaaat 
      catcgtcag ttgtcgatc tctcagcca ctacgatgg 
      gcgaacaat taaaacatt gctacctca aatttttgc 
      aacagtatt gtccgcttc atttcacat agtggtcgg 
      aatatcttt ggctttttc cggcatact tttacccca 
      acttgacga cgaaaaatt cccgattac gccacaaca 
      aaggccatg gattgcaag tgtgatagt tttggctgt 
      gccacttgg cgccgtcgc tggtggctt tataatgga 
      cgctatagg aagactgaa tactgttaa gttggcagc 
      aatacctgg ggttttcgg atggacgtt aatcgccat 
      ggctaccaa cttccacat gctgataat cgggcggct 
      tttaacagg actggcgtc agctttggg gaccagtcc 
      tgccatcgt ctacctgac ggaaattgc aagggctga 
      catgagagg ctcgttgat ttccttcgc ccccgcata 
      tgcctcctt aggtatggt acttacatt tttgaaagg 
      ttggttctt cagttggcg cgtcgttgc atggacttg 
      tctcggcta cactgtaat tccttgtgt tctactaat 
      gttcatacc tgaaagtcc cgcttggtt ggtatcgaa 
      aggaaaaat cgaacaagc gagtaaatc actggcttg 
      gatcaacaa gtaccaacc acaacccga aaataaacc 
      acaaacttt agccgaaat gcagttggc acaactgca 
      aaaagaaca ccaaaagaa actagacga ggcagccct 
      gcatggccg gggggcggc ctacaaggc ccgcgcgtt 
      cctcaaacc cacgggcta caagccgtt gctgatcct 
      gattggttt attcttatg tcaacagtt ttcggggat 
      ttacatcac cctgttcca ctcggtcga gtttttcca 
      ggcggttgg tagtcctgt gaacgcgta cttggcctc 
      tgtcctcat cagcaccgt gcgtctttt tatgtcagt 
      gcttgacac ttacctcct gcgcacgtt cagccggcg 
      cccccttat catgttgag tggcttcac catggccac 
      gtgcatgtt tttttcggg gcttttcac caagtggat 
      caccgaggg gaccactga cgccacgtg ggtccccgt 
      ggcctttct tttacttta cgtcatagc gtcaatgtt 
      ggggctttt acccattcc gtggacgat gacagccga 
      actgttccc gattgagat aaggggcgt ggctcacag 
      tatcgcata cagtagtgc caacatact catgtttgg 
      ggccgtcca atgctacga agtgctaat gacctcgct 
      taagggggc tgctggagt gcagttttt cttcgctgt 
      gatttgcat tattgcaat ggtttatac gtttgtgtt 
      cgttcccga aacgcaccg gaagaagtt gacggaaat 
      tgaggagta tttcaacca caatatgat ctatttagg 
      gcagaagcc ccagaaagg ggggaagaa ggacaagag 
      tagggacaa ggaacaaac agagaagtt gatgaaagt 
      ttgaatctc gagtattat tagttgtaa ggcaaagct 
      gatattttt gtaaggatt tacgaactg tgctttaag 
      acaattagt gttgtttga tttatttag agacaaact 
      ttgtgattt tgtataaca aatattaca ataaaatta 
      tttaacttg attttttcg ttttcttgc gggtgtgat 
      aatcgaatt gaggcggca atgtaatat gcatttgtt 
      acacttgga ccaatcaga cgcctcctt caagcccac 
      tttgccact cctttcaaa tttaacctt acaaactga 
      acaaacata caaaacttt ggtgacaaa aatactgaa 
      aaaaagcaa taaaaaaag acaaaatga ctcaaactg 
      aaaaaacct gttaattgt ttcttcctc tgattcaac 
      aattacaac atcactgtc cctactact gtcgtcctc 
      taacatggg ttgtttttt acctgaaaa ttaacatca 
      atcgaatgt cataattcg ttttggaaa tacgtttgg 
      tgcccgggt agttccccc gttttcgct tcggtgcga 
      aaaatttcg atttgaaaa cctgactta gtctcgagt 
      ggagaatac atttattac acttgtaca aatcagact 
      cgtgccaaa acttaacac ttgtgcccc tctgaaaac 
      cacatttac acattgaac acactggga gtctctcct 
      caattatga cacttgtcg ggggctctt ggacacacg 
      tttcctcaa ctttgacct catcctcaa tcaggatgt 
      aatcctcag cgggaactt cctttttaa tcgacttag 
      aatccataa aaaattaaa aattgtagg aaaatagtg 
      aggttatat cattggcgt gtataatgt gctgcctac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY