Status
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Gene information
General information
TC015805 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8668394..8690925
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015805
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05899

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015805 RA
      MVSRLFLPQ PLSRTLKSL VLLTLITAT FSDKELRNR 
      SNSIVKRRF TDASFDEVS TQASGCGSC KMREEIKNR 
      NLEVIKGEV LRRMGFQTA PNVTGRVLP PVPPHFLAK 
      VDLEMAGMQ SDEPLFKTG YSFTEEEDD YHVKTQEVL 
      TFAQPYPRL RHSWRGHDI LHFMFSDGI TKYHVSNAT 
      LYVYMKGAE RRPLPDVLI EVFKVYKAP DHPDTPGLY 
      RMVSKKVTQ PYGRGDWVK LDLTITVSE WFKSPRENH 
      GFVVNATVN GKKVVVTDT TLDNGSKAP FVEVSTMEA 
      RRRTRRNVG LNCDDKMNE PLCCRYPLT VDFEEFGWD 
      FIIAPKRYD AHYCSGECP YVTLQKYPH THLMKMASP 
      NSAQPCCAP RKMSAISML YFDNQLNVV YGSLPGMVV 
      DRCGCS
    CDS sequence
    • >TC015805 RA
      atggtaagc cggcttttc ctcccccaa cccttgtcg 
      cggacgtta aaatcgctc gtgctcctg actctgata 
      acggcgacg tttagtgat aaagagctc cgcaaccgc 
      tccaacagt attgtaaaa cgacgcttt acggacgcc 
      tccttcgac gaggtgtcc acccaggcc tcgggctgc 
      ggctcgtgc aaaatgcgc gaggagatc aagaaccgc 
      aacctggag gtgatcaag ggggaggtg ttgcgccgg 
      atgggcttc cagaccgcc cccaacgtc accggcagg 
      gtgctgccc ccggtgccc ccgcatttc ctggccaag 
      gtggacctg gagatggcc ggcatgcag agcgacgag 
      cccctcttc aaaacgggc tactccttc accgaggag 
      gaggacgac taccacgtc aaaacgcaa gaagtgctc 
      acctttgca caaccatat ccaagactg aggcacagc 
      tggcgcggc cacgacatc ctccacttc atgttctct 
      gacggcatc accaagtac cacgtgagc aacgccacc 
      ctgtacgtg tacatgaag ggcgccgag cgtcggccc 
      ctccccgac gtcctcatc gaagtgttc aaagtgtac 
      aaagcgccc gaccatccc gacactcca ggcctctac 
      aggatggtc agcaagaaa gtcacccag ccgtacggt 
      aggggagac tgggtcaag ctggacctc accatcacc 
      gtctccgag tggttcaaa agtccgcgc gagaatcac 
      ggcttcgtg gttaatgcg acggttaat gggaaaaaa 
      gtcgtggtc acggacacg acgctagat aatggcagt 
      aaggcgcct ttcgtggag gtgtccacg atggaggcc 
      cgccgacgc acgcgccgc aacgtgggg ctcaactgc 
      gacgacaag atgaacgag cccctctgc tgccgctac 
      ccgctcacc gtggacttc gaggagttc gggtgggac 
      ttcatcatc gctccgaaa cggtacgac gcgcactac 
      tgctccggc gagtgtccg tacgtcacg ctgcagaag 
      tacccccac acccacctc atgaagatg gcgtcgccc 
      aacagcgcc cagccctgc tgcgccccc cgcaagatg 
      agcgccatc tccatgctc tacttcgac aaccagctc 
      aacgtcgtc tacggcagc ctgccgggg atggtggtc 
      gaccggtgc gggtgctcg taa
    mRNA sequence
    • >TC015805 RA
      gtgttggtc tttaactca aaccaactc ggtttattt 
      actttactg atagatctc ttctgaagc cgacacgcg 
      tgcgaatgt gataccaag ctagaataa tttttcccc 
      aatgtcaaa aaaattcga ttgtgatca aaattgtga 
      ttaaaattt ttcgaaaaa aaaaaaaaa attcgaaat 
      tcgatgtga tttcacccc gaaagtatt atgggcaat 
      agtagtgtg aacccttta aaaatgtga taaaaagtg 
      gacgcattg tgatacacc ccgtttgtg atacaccac 
      cgactgatt actttagaa caagcaaga tggtaagcc 
      ggcttttcc tcccccaac ccttgtcgc ggacgttaa 
      aatcgctcg tgctcctga ctctgataa cggcgacgt 
      ttagtgata aagagctcc gcaaccgct ccaacagta 
      ttgtaaaac gacgcttta cggacgcct ccttcgacg 
      aggtgtcca cccaggcct cgggctgcg gctcgtgca 
      aaatgcgcg aggagatca agaaccgca acctggagg 
      tgatcaagg gggaggtgt tgcgccgga tgggcttcc 
      agaccgccc ccaacgtca ccggcaggg tgctgcccc 
      cggtgcccc cgcatttcc tggccaagg tggacctgg 
      agatggccg gcatgcaga gcgacgagc ccctcttca 
      aaacgggct actccttca ccgaggagg aggacgact 
      accacgtca aaacgcaag aagtgctca cctttgcac 
      aaccatatc caagactga ggcacagct ggcgcggcc 
      acgacatcc tccacttca tgttctctg acggcatca 
      ccaagtacc acgtgagca acgccaccc tgtacgtgt 
      acatgaagg gcgccgagc gtcggcccc tccccgacg 
      tcctcatcg aagtgttca aagtgtaca aagcgcccg 
      accatcccg acactccag gcctctaca ggatggtca 
      gcaagaaag tcacccagc cgtacggta ggggagact 
      gggtcaagc tggacctca ccatcaccg tctccgagt 
      ggttcaaaa gtccgcgcg agaatcacg gcttcgtgg 
      ttaatgcga cggttaatg ggaaaaaag tcgtggtca 
      cggacacga cgctagata atggcagta aggcgcctt 
      tcgtggagg tgtccacga tggaggccc gccgacgca 
      cgcgccgca acgtggggc tcaactgcg acgacaaga 
      tgaacgagc ccctctgct gccgctacc cgctcaccg 
      tggacttcg aggagttcg ggtgggact tcatcatcg 
      ctccgaaac ggtacgacg cgcactact gctccggcg 
      agtgtccgt acgtcacgc tgcagaagt acccccaca 
      cccacctca tgaagatgg cgtcgccca acagcgccc 
      agccctgct gcgcccccc gcaagatga gcgccatct 
      ccatgctct acttcgaca accagctca acgtcgtct 
      acggcagcc tgccgggga tggtggtcg accggtgcg 
      ggtgctcgt aatcgaccc gattcttaa ttgctttat 
      ttattcgtg atagacgca agtgaactg acgatttgt 
      tgtaaattt ttgtacata atatgtgat acgtgtagt 
      ttaattttt tttaatatt tatttttgt acattagct 
      agcaaaatt cagtgagtt ttgagttcg agaaacgat 
      gagtgataa attgctgca caattgcgt tgcttttgt 
      gatataatg gccttacga gtgttgttt acacgggga 
      tacatattt ttcgaataa tctttcacg gttttgtga 
      taaaatttt gcccaaatt tctactcat gatgtaaca 
      tagttttgt aaataagac tggccttag atttgtctt 
      ctgtggtag gatctcttt tttgtttgt tttgttttg 
      tggctctaa ttttagacg ctgaaattt gactttttt 
      ctacatggt ctttctttc acttgtttt ctttatgag 
      tattgcctg atttttcta tcgtgtgtc tgaataaat 
      gtcgaaagt ttcgaaaac aatagccaa aaattaaat 
      atttgtaat tattttttt taaatagtt agctaatta 
      ccacccatc tgaaattat cagaaccct cctgactag 
      acatttatt tagacacac ggtatatgt ctgtcgtgt 
      gttttgctt ttgtaattg taaattagt tgtaattaa 
      tagcatttt gcaaaataa ataaattta cataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY