| General information | |||
| TC033450
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 8297911..8422372 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033450 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05895 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
leg musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
maxilla shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
legs shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
legs femur potentially fused - Penetrance (percentage of animals/eggs on slide): more than 80%
legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): more than 80%
urogomphi shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC033450 RA
MRFDTTLEP PLDAKVKTT MAYHPFLQL PRQTDFSVS SLLTAASSS NPTSPPAPG FFPSAALAA FGSQASHGC YPGTLIPKI SHPHPHHAL SGHPYTTAE DVVLAAVAA HNHHPGMVR PLRAIQPEE DGVVDDPKV TLEGKDLWE KFHKLGTEM VITKSGRQM FPQMKFRVS GLDLKAKYI LLLDIVAAD DYRYKFHNS RWMVAGKAD PEMPKRMYI HPDSPSTGE QWMQKVVSF HKLKLTNNI SDKHGFLKE KAVDFLTIL NSMHKYQPR FHLVRANDI LKLPYSTFR TYVFKETEF IAVTAYQNE KITQLKIDN NPFAKGFRD TGAGKREKK QAMLAQRHT DDKTSHPLP ARMSADSST VSKTDDSEL DVVGTADSP RPSLHASTP IASSLNHSG PEDDSLRRR LHDDSGSES SCSESPGAF RPRPSSPKE TTAGPSNPS GEYPSPNIS VGPPIHPPP HLLPYLYPP GLYPGAGGP PFAPHLNLF SGAHAPGMN PSLLFNAQL ALAAQHPAL FSHYQNLAH PNPHHLSPT SPLHNHLKG GHRFTPYTL PTSNSGVSS SPLGSAFET VTPSSHTRS LSNSPSGKL RGGSMSPPV RPSTASPKP EAATSPSDL KSIEKMVNG LDVKQNEDN DK
- >TC033450 RB
MRFDTTLEP PLDAKVKTT MAYHPFLQL PRQTDFSVS SLLTAASSS NPTSPPAPG FFPSAALAA FGSQASHGC YPGTLIPKI SHPHPHHAL SGHPYTTAE DVVLAAVAA HNHHPGMVR PLRAIQPEE DGVVDDPKV TLEGKDLWE KFHKLGTEM VITKSGRQM FPQMKFRVS GLDLKAKYI LLLDIVAAD DYRYKFHNS RWMVAGKAD PEMPKRMYI HPDSPSTGE QWMQKVVSF HKLKLTNNI SDKHGFTIL NSMHKYQPR FHLVRANDI LKLPYSTFR TYVFKETEF IAVTAYQNE KITQLKIDN NPFAKGFRD TGAGKREKK QAMLAQRHT DDKTSHPLP ARMSADSST VSKTDDSEL DVVGTADSP RPSLHASTP IASSLNHSG PEDDSLRRR LHDDSGSES SCSESPGAF RPRPSSPKE TTAGPSNPS GEYPSPNIS VGPPIHPPP HLLPYLYPP GLYPGAGGP PFAPHLNLF SGAHAPGMN PSLLFNAQL ALAAQHPAL FSHYQNLAH PNPHHLSPT SPLHNHLKG GHRFTPYTL PTSNSGVSS SPLGSAFET VTPSSHTRS LSNSPSGKL RGGSMSPPV RPSTASPKP EAATSPSDL KSIEKMVNG LDVKQNEDN DK
- >TC033450 RA
atgcgtttc gatacgacc ctggagcca cccctcgac gccaaagtg aagaccacg atggcctac caccccttc ctgcagctg ccgcgccag acggacttc agcgtcagc tcgctgctc accgccgcc tccagcagc aaccccacg tcgccgccc gcccccggc ttcttcccg tcggcggcg ctcgccgcc ttcggctcg caggcctcg cacggctgc tacccgggc acgctcata cccaagatc agccatccg cacccgcat cacgccctc tcgggacac ccctacacc accgccgag gacgtggtg ctggccgcc gtcgccgcg cacaaccac cacccgggg atggtcagg ccgctgagg gcgatccag ccggaggag gatggggtg gtcgacgat cccaaagtc acacttgaa gggaaagat ctctgggaa aagtttcat aaactcggc acggaaatg gtcatcacc aaaagtggg agacaaatg tttccccag atgaagttc agagtgtcc ggtttggac ctgaaagcc aaatacata ctcctgttg gacatagtc gccgctgac gactacaga tacaaattc cataacagc cggtggatg gtggcgggc aaggccgac cccgaaatg cccaaacgg atgtacatc cacccagac agcccctcg accggcgag cagtggatg cagaaagtc gtctccttc cacaagctc aagctcacc aacaacatc tcggacaag cacggcttt ttgaaagaa aaagcggtt gatttcctg acgatactc aactccatg cacaaatac cagcctcgg tttcacctc gttagggcc aacgacatt ttgaagttg ccctactcg acgtttcgg acgtacgtc ttcaaggaa acggagttc atcgcagtg acagcgtac caaaacgaa aagataacg cagctcaag atcgacaac aatccgttc gcgaagggg ttcagggat acgggggcg ggcaagagg gagaaaaag caggcgatg ctggcgcag agacacacc gatgataag acgtcacat ccgcttcct gcgaggatg tcggcggac agcagcacc gtatcaaag actgatgac tcggaattg gatgttgtg gggacggcg gacagtccg aggccgtcc ctgcacgcc tccacgccg attgcgtct tcgcttaat cattccggc cccgaagac gactccctt cgccggcgc ctccacgac gactccggc tctgaatcc tcgtgttca gaatccccc ggagccttc cggccgcgg ccctcctcc cccaaggag accaccgcc ggcccctcc aacccctct ggcgagtac ccctcgccc aacatcagc gtgggcccc ccaatccac cccccgcca cacctgctc ccgtacctg tacccccct ggcctgtac cccggcgcg gggggcccg cctttcgcc ccccatttg aacctcttt tccggggcg cacgccccc ggcatgaac cccagcctc ctcttcaac gctcaacta gccctcgcc gcccaacac cccgccctc ttctcccac taccaaaac ctagcgcac cccaacccc caccacttg tcccccacc tccccgctg cataaccac ctcaagggg gggcacagg ttcaccccc tacacgctg cccacctcc aactcgggg gtgagttcg tcccccttg gggagtgcg ttcgagacg gtcaccccc agctcgcac acgaggagt ttgagtaat tctccctcg gggaagctg aggggcggg tcgatgtcg cccccagtg aggcccagt accgcctcc cccaagccg gaggcggcg acctccccc agcgacctc aagagtata gaaaaaatg gtaaatgga ctcgatgtt aagcaaaac gaagacaat gacaagtag
- >TC033450 RB
atgcgtttc gatacgacc ctggagcca cccctcgac gccaaagtg aagaccacg atggcctac caccccttc ctgcagctg ccgcgccag acggacttc agcgtcagc tcgctgctc accgccgcc tccagcagc aaccccacg tcgccgccc gcccccggc ttcttcccg tcggcggcg ctcgccgcc ttcggctcg caggcctcg cacggctgc tacccgggc acgctcata cccaagatc agccatccg cacccgcat cacgccctc tcgggacac ccctacacc accgccgag gacgtggtg ctggccgcc gtcgccgcg cacaaccac cacccgggg atggtcagg ccgctgagg gcgatccag ccggaggag gatggggtg gtcgacgat cccaaagtc acacttgaa gggaaagat ctctgggaa aagtttcat aaactcggc acggaaatg gtcatcacc aaaagtggg agacaaatg tttccccag atgaagttc agagtgtcc ggtttggac ctgaaagcc aaatacata ctcctgttg gacatagtc gccgctgac gactacaga tacaaattc cataacagc cggtggatg gtggcgggc aaggccgac cccgaaatg cccaaacgg atgtacatc cacccagac agcccctcg accggcgag cagtggatg cagaaagtc gtctccttc cacaagctc aagctcacc aacaacatc tcggacaag cacggcttt acgatactc aactccatg cacaaatac cagcctcgg tttcacctc gttagggcc aacgacatt ttgaagttg ccctactcg acgtttcgg acgtacgtc ttcaaggaa acggagttc atcgcagtg acagcgtac caaaacgaa aagataacg cagctcaag atcgacaac aatccgttc gcgaagggg ttcagggat acgggggcg ggcaagagg gagaaaaag caggcgatg ctggcgcag agacacacc gatgataag acgtcacat ccgcttcct gcgaggatg tcggcggac agcagcacc gtatcaaag actgatgac tcggaattg gatgttgtg gggacggcg gacagtccg aggccgtcc ctgcacgcc tccacgccg attgcgtct tcgcttaat cattccggc cccgaagac gactccctt cgccggcgc ctccacgac gactccggc tctgaatcc tcgtgttca gaatccccc ggagccttc cggccgcgg ccctcctcc cccaaggag accaccgcc ggcccctcc aacccctct ggcgagtac ccctcgccc aacatcagc gtgggcccc ccaatccac cccccgcca cacctgctc ccgtacctg tacccccct ggcctgtac cccggcgcg gggggcccg cctttcgcc ccccatttg aacctcttt tccggggcg cacgccccc ggcatgaac cccagcctc ctcttcaac gctcaacta gccctcgcc gcccaacac cccgccctc ttctcccac taccaaaac ctagcgcac cccaacccc caccacttg tcccccacc tccccgctg cataaccac ctcaagggg gggcacagg ttcaccccc tacacgctg cccacctcc aactcgggg gtgagttcg tcccccttg gggagtgcg ttcgagacg gtcaccccc agctcgcac acgaggagt ttgagtaat tctccctcg gggaagctg aggggcggg tcgatgtcg cccccagtg aggcccagt accgcctcc cccaagccg gaggcggcg acctccccc agcgacctc aagagtata gaaaaaatg gtaaatgga ctcgatgtt aagcaaaac gaagacaat gacaagtag
- >TC033450 RA
cacgtgacg tgtccgcta atgaaaacc gtgtgtttt tgcaaatgt gtatgtgtg tgtgtgtgc ggcgcttaa aaaggtcga gcgttggtg gcgtctgaa cagcatgcg gtgaattca tgcatatgc gtcccgttt ggtaaagga gcattcagt cctggcaac atcaatgtc gcctcagca gaaaaagga aaccgtgtg acaaaaagc actcgtaac gcctccata ggcataacc tcctaactc ctgcccacc gtaagtgtg aaaagtggg ggcgaaatg tcgccatcg gattggcgg agccgcttg agctgacac cctcgcgac caatcccat cgcgtccgt gtcattaac acagtcagt gtcgaatct gcgggtttc ctgctatag tcgtcgctc acacttaac ccgggaagg aggtgtcag acttcacac tcaagccac cccgcgttc aggacctac tcagtgcag tgcgtggtg cagtgcggt gatgtgata tacagggcc ggcgtcgga ccatgcgtt tcgatacga ccctggagc cacccctcg acgccaaag tgaagacca cgatggcct accacccct tcctgcagc tgccgcgcc agacggact tcagcgtca gctcgctgc tcaccgccg cctccagca gcaacccca cgtcgccgc ccgcccccg gcttcttcc cgtcggcgg cgctcgccg ccttcggct cgcaggcct cgcacggct gctacccgg gcacgctca tacccaaga tcagccatc cgcacccgc atcacgccc tctcgggac acccctaca ccaccgccg aggacgtgg tgctggccg ccgtcgccg cgcacaacc accacccgg ggatggtca ggccgctga gggcgatcc agccggagg aggatgggg tggtcgacg atcccaaag tcacacttg aagggaaag atctctggg aaaagtttc ataaactcg gcacggaaa tggtcatca ccaaaagtg ggagacaaa tgtttcccc agatgaagt tcagagtgt ccggtttgg acctgaaag ccaaataca tactcctgt tggacatag tcgccgctg acgactaca gatacaaat tccataaca gccggtgga tggtggcgg gcaaggccg accccgaaa tgcccaaac ggatgtaca tccacccag acagcccct cgaccggcg agcagtgga tgcagaaag tcgtctcct tccacaagc tcaagctca ccaacaaca tctcggaca agcacggct ttttgaaag aaaaagcgg ttgatttcc tgacgatac tcaactcca tgcacaaat accagcctc ggtttcacc tcgttaggg ccaacgaca ttttgaagt tgccctact cgacgtttc ggacgtacg tcttcaagg aaacggagt tcatcgcag tgacagcgt accaaaacg aaaagataa cgcagctca agatcgaca acaatccgt tcgcgaagg ggttcaggg atacggggg cgggcaaga gggagaaaa agcaggcga tgctggcgc agagacaca ccgatgata agacgtcac atccgcttc ctgcgagga tgtcggcgg acagcagca ccgtatcaa agactgatg actcggaat tggatgttg tggggacgg cggacagtc cgaggccgt ccctgcacg cctccacgc cgattgcgt cttcgctta atcattccg gccccgaag acgactccc ttcgccggc gcctccacg acgactccg gctctgaat cctcgtgtt cagaatccc ccggagcct tccggccgc ggccctcct cccccaagg agaccaccg ccggcccct ccaacccct ctggcgagt acccctcgc ccaacatca gcgtgggcc ccccaatcc accccccgc cacacctgc tcccgtacc tgtaccccc ctggcctgt accccggcg cggggggcc cgcctttcg ccccccatt tgaacctct tttccgggg cgcacgccc ccggcatga accccagcc tcctcttca acgctcaac tagccctcg ccgcccaac accccgccc tcttctccc actaccaaa acctagcgc accccaacc cccaccact tgtccccca cctccccgc tgcataacc acctcaagg gggggcaca ggttcaccc cctacacgc tgcccacct ccaactcgg gggtgagtt cgtccccct tggggagtg cgttcgaga cggtcaccc ccagctcgc acacgagga gtttgagta attctccct cggggaagc tgaggggcg ggtcgatgt cgcccccag tgaggccca gtaccgcct cccccaagc cggaggcgg cgacctccc ccagcgacc tcaagagta tagaaaaaa tggtaaatg gactcgatg ttaagcaaa acgaagaca atgacaagt agtgaaagt gatcctaag ttttagttg gccgtactg atgatatat acggggtgt ttttgcaat agattcttt tggattttt gtgattttt gcacatgaa attaccgta aagtgcttt tcttattgt agcttacaa aaaaagtac aaaatgtat gtgaattga ggcagattt tttcgttta atccgacct tatttggtg ctatgctca ttctgtttg accctagaa aggaagaaa gtctggaag aggggtcac ttttagact ttgttcctc ctcaattag tgatcctgt agactgttg aagcttttg tataataat aatgatatt tactaagag acacgatag aagagttaa tgttccttt tactattat tgcaattaa tttaaataa ttatgtaaa tagtgtttg tacgtaaac gcgtaaaca tctgtatat aatggttgt tttttgtgt tttatgaag aaaaactgt aaattgtaa attgctcct ataactact agaactgta tattagaac tcaaaatta atttatatt tctaccaaa gaatttcga tggtttttg caccaacag ctgggcaaa aaccaccag aaatagtga atatagcaa taaagaagt acataaacc ataaacctt ctctcgaaa ctctcaaca ttgtacata aatatagtt taaatgtac atttcttta ttgacatgc ttttcaaat cctacttct tctgtttat atcgttgag cagtaccag cgttaaaat aacttgttt ctatttatt atcaaaagt tttatattc aaatactat catcgctta acgctggta cttggaaat tattaaaat ctgtattaa agtagaaga tgtgaaatt tgtcttgtg tatatgtgg aatactttt aagaattaa acgatattc atacat
- >TC033450 RB
cacgtgacg tgtccgcta atgaaaacc gtgtgtttt tgcaaatgt gtatgtgtg tgtgtgtgc ggcgcttaa aaaggtcga gcgttggtg gcgtctgaa cagcatgcg gtgaattca tgcatatgc gtcccgttt ggtaaagga gcattcagt cctggcaac atcaatgtc gcctcagca gaaaaagga aaccgtgtg acaaaaagc actcgtaac gcctccata ggcataacc tcctaactc ctgcccacc gtaagtgtg aaaagtggg ggcgaaatg tcgccatcg gattggcgg agccgcttg agctgacac cctcgcgac caatcccat cgcgtccgt gtcattaac acagtcagt gtcgaatct gcgggtttc ctgctatag tcgtcgctc acacttaac ccgggaagg aggtgtcag acttcacac tcaagccac cccgcgttc aggacctac tcagtgcag tgcgtggtg cagtgcggt gatgtgata tacagggcc ggcgtcgga ccatgcgtt tcgatacga ccctggagc cacccctcg acgccaaag tgaagacca cgatggcct accacccct tcctgcagc tgccgcgcc agacggact tcagcgtca gctcgctgc tcaccgccg cctccagca gcaacccca cgtcgccgc ccgcccccg gcttcttcc cgtcggcgg cgctcgccg ccttcggct cgcaggcct cgcacggct gctacccgg gcacgctca tacccaaga tcagccatc cgcacccgc atcacgccc tctcgggac acccctaca ccaccgccg aggacgtgg tgctggccg ccgtcgccg cgcacaacc accacccgg ggatggtca ggccgctga gggcgatcc agccggagg aggatgggg tggtcgacg atcccaaag tcacacttg aagggaaag atctctggg aaaagtttc ataaactcg gcacggaaa tggtcatca ccaaaagtg ggagacaaa tgtttcccc agatgaagt tcagagtgt ccggtttgg acctgaaag ccaaataca tactcctgt tggacatag tcgccgctg acgactaca gatacaaat tccataaca gccggtgga tggtggcgg gcaaggccg accccgaaa tgcccaaac ggatgtaca tccacccag acagcccct cgaccggcg agcagtgga tgcagaaag tcgtctcct tccacaagc tcaagctca ccaacaaca tctcggaca agcacggct ttacgatac tcaactcca tgcacaaat accagcctc ggtttcacc tcgttaggg ccaacgaca ttttgaagt tgccctact cgacgtttc ggacgtacg tcttcaagg aaacggagt tcatcgcag tgacagcgt accaaaacg aaaagataa cgcagctca agatcgaca acaatccgt tcgcgaagg ggttcaggg atacggggg cgggcaaga gggagaaaa agcaggcga tgctggcgc agagacaca ccgatgata agacgtcac atccgcttc ctgcgagga tgtcggcgg acagcagca ccgtatcaa agactgatg actcggaat tggatgttg tggggacgg cggacagtc cgaggccgt ccctgcacg cctccacgc cgattgcgt cttcgctta atcattccg gccccgaag acgactccc ttcgccggc gcctccacg acgactccg gctctgaat cctcgtgtt cagaatccc ccggagcct tccggccgc ggccctcct cccccaagg agaccaccg ccggcccct ccaacccct ctggcgagt acccctcgc ccaacatca gcgtgggcc ccccaatcc accccccgc cacacctgc tcccgtacc tgtaccccc ctggcctgt accccggcg cggggggcc cgcctttcg ccccccatt tgaacctct tttccgggg cgcacgccc ccggcatga accccagcc tcctcttca acgctcaac tagccctcg ccgcccaac accccgccc tcttctccc actaccaaa acctagcgc accccaacc cccaccact tgtccccca cctccccgc tgcataacc acctcaagg gggggcaca ggttcaccc cctacacgc tgcccacct ccaactcgg gggtgagtt cgtccccct tggggagtg cgttcgaga cggtcaccc ccagctcgc acacgagga gtttgagta attctccct cggggaagc tgaggggcg ggtcgatgt cgcccccag tgaggccca gtaccgcct cccccaagc cggaggcgg cgacctccc ccagcgacc tcaagagta tagaaaaaa tggtaaatg gactcgatg ttaagcaaa acgaagaca atgacaagt agtgaaagt gatcctaag ttttagttg gccgtactg atgatatat acggggtgt ttttgcaat agattcttt tggattttt gtgattttt gcacatgaa attaccgta aagtgcttt tcttattgt agcttacaa aaaaagtac aaaatgtat gtgaattga ggcagattt tttcgttta atccgacct tatttggtg ctatgctca ttctgtttg accctagaa aggaagaaa gtctggaag aggggtcac ttttagact ttgttcctc ctcaattag tgatcctgt agactgttg aagcttttg tataataat aatgatatt tactaagag acacgatag aagagttaa tgttccttt tactattat tgcaattaa tttaaataa ttatgtaaa tagtgtttg tacgtaaac gcgtaaaca tctgtatat aatggttgt tttttgtgt tttatgaag aaaaactgt aaattgtaa attgctcct ataactact agaactgta tattagaac tcaaaatta atttatatt tctaccaaa gaatttcga tggtttttg caccaacag ctgggcaaa aaccaccag aaatagtga atatagcaa taaagaagt acataaacc ataaacctt ctctcgaaa ctctcaaca ttgtacata aatatagtt taaatgtac atttcttta ttgacatgc ttttcaaat cctacttct tctgtttat atcgttgag cagtaccag cgttaaaat aacttgttt ctatttatt atcaaaagt tttatattc aaatactat catcgctta acgctggta cttggaaat tattaaaat ctgtattaa agtagaaga tgtgaaatt tgtcttgtg tatatgtgg aatactttt aagaattaa acgatattc atacat
