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Gene information
General information
TC015784 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8078856..8080489
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015784
    iBeetle screen iB_05894

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015784 RA
      MAKILPFCA LFLVTLIVI YLWNVSNSI NYELEAKEE 
      TLLLSDYER SQRGDPSNF SQILQQVKH ITGCLDRPM 
      EPKIQQRGD YWVLYNYIV AQKRFKCHE SVTITTLGD 
      FTFLDNILP MVERWKGPI SVALHAPGT DFNAALDAI 
      KHLRCHSAL VREFVTFHI FFPSGHMPP KNLKTYDFE 
      DDHECPETL LLPNSTYKS AHQLVFPIN LARNIAREM 
      AQTHFIFPL DIELYPSPY LIPKFLKMI AQNGPWATP 
      RVYPLAVFE IETGQKIPD NKTQVQAML AKNLAVPFH 
      HNICAQCHD IPKALKWQK TAETPGLRV FYSAKRKGE 
      YVHWEPFYI TTHQEPLYD ERFTWEGQK DKLVQGFVL 
      CILDYDFMI LDNAFLVHK PGIKVKTMD ERRQAMTDL 
      TDMSIKTEI MPQLEILFG RRGGCEV
    CDS sequence
    • >TC015784 RA
      atggcaaaa atactccca ttttgcgcc cttttcctt 
      gtgacacta atagttatt tacttgtgg aacgtgtca 
      aattccata aactacgaa ctggaagcc aaggaagag 
      actttatta ctgtccgac tatgagaga agccagcga 
      ggtgacccc tcaaacttt tctcaaatt ctgcaacaa 
      gtgaaacac ataaccggg tgtctggac cgacccatg 
      gaacccaag atccaacaa agaggcgac tattgggtg 
      ctgtacaac tacatagtg gcccaaaaa cggttcaag 
      tgccacgag agtgtgacc ataaccacg ttgggcgac 
      ttcaccttc cttgataat atcttacca atggtggaa 
      cgctggaaa gggcccata agtgtcgcc cttcatgcc 
      cccggcact gacttcaac gccgccttg gacgcgatt 
      aaacacctg cgttgccac agcgccctt gtcagggaa 
      tttgtgacc tttcatatt tttttcccg agcggccac 
      atgccgcca aaaaattta aaaacgtac gactttgaa 
      gacgaccac gaatgtccc gaaacgttg cttttgccc 
      aactcgacc tacaagagc gcccaccag ctcgttttt 
      ccgataaat ttagctcga aatattgca cgtgaaatg 
      gcacagacc cactttatt tttcccttg gacatcgaa 
      ctgtacccc agcccttac ctcatcccc aaatttctc 
      aaaatgatc gcacagaac ggcccttgg gccacgccc 
      cgtgtgtac ccccttgcg gtctttgaa atcgaaact 
      gggcaaaaa atcccagac aataaaacg caagtccag 
      gcaatgctg gcgaaaaat ttggccgtg cccttccac 
      cacaacatt tgtgcccaa tgtcacgac attcccaag 
      gcccttaag tggcaaaaa acggccgaa acgcccggt 
      ttgcgcgtg ttttactcg gcgaaacga aagggagag 
      tatgtgcat tgggagccc ttctacatc accacgcac 
      caggaacca ctgtatgac gagaggttc acgtgggaa 
      ggccagaag gataagctg gttcaaggg ttcgttttg 
      tgtatcctg gactatgat tttatgatc ctggataat 
      gcctttttg gtgcataag ccgggcatt aaagtgaag 
      acgatggac gagaggagg caagccatg accgacttg 
      actgatatg agcattaaa accgagatt atgccacag 
      ctggagatt ttgtttggg aggagagga gggtgcgag 
      gtgtga
    mRNA sequence
    • >TC015784 RA
      atccacttg caaagtaag gtgccctaa agcagaatt 
      tccaaaaat cttcaaaat tacaagtag gttacaatt 
      ttatttgaa caccttatc aagtccgtt ttgaataaa 
      ttttatcag acgacgtcc tttattgat caattctgt 
      tatcaaaaa atcaattcc tatcattgt tttatcagc 
      ccagtataa aatgaaatt tcgtcacaa atggcaaaa 
      atactccca ttttgcgcc cttttcctt gtgacacta 
      atagttatt tacttgtgg aacgtgtca aattccata 
      aactacgaa ctggaagcc aaggaagag actttatta 
      ctgtccgac tatgagaga agccagcga ggtgacccc 
      tcaaacttt tctcaaatt ctgcaacaa gtgaaacac 
      ataaccggg tgtctggac cgacccatg gaacccaag 
      atccaacaa agaggcgac tattgggtg ctgtacaac 
      tacatagtg gcccaaaaa cggttcaag tgccacgag 
      agtgtgacc ataaccacg ttgggcgac ttcaccttc 
      cttgataat atcttacca atggtggaa cgctggaaa 
      gggcccata agtgtcgcc cttcatgcc cccggcact 
      gacttcaac gccgccttg gacgcgatt aaacacctg 
      cgttgccac agcgccctt gtcagggaa tttgtgacc 
      tttcatatt tttttcccg agcggccac atgccgcca 
      aaaaattta aaaacgtac gactttgaa gacgaccac 
      gaatgtccc gaaacgttg cttttgccc aactcgacc 
      tacaagagc gcccaccag ctcgttttt ccgataaat 
      ttagctcga aatattgca cgtgaaatg gcacagacc 
      cactttatt tttcccttg gacatcgaa ctgtacccc 
      agcccttac ctcatcccc aaatttctc aaaatgatc 
      gcacagaac ggcccttgg gccacgccc cgtgtgtac 
      ccccttgcg gtctttgaa atcgaaact gggcaaaaa 
      atcccagac aataaaacg caagtccag gcaatgctg 
      gcgaaaaat ttggccgtg cccttccac cacaacatt 
      tgtgcccaa tgtcacgac attcccaag gcccttaag 
      tggcaaaaa acggccgaa acgcccggt ttgcgcgtg 
      ttttactcg gcgaaacga aagggagag tatgtgcat 
      tgggagccc ttctacatc accacgcac caggaacca 
      ctgtatgac gagaggttc acgtgggaa ggccagaag 
      gataagctg gttcaaggg ttcgttttg tgtatcctg 
      gactatgat tttatgatc ctggataat gcctttttg 
      gtgcataag ccgggcatt aaagtgaag acgatggac 
      gagaggagg caagccatg accgacttg actgatatg 
      agcattaaa accgagatt atgccacag ctggagatt 
      ttgtttggg aggagagga gggtgcgag gtgtgaaaa 
      tgataagct gaatttgtg ataaaaatc ttttattga 
      taagaatta ataaaataa ataaatagt gatacaata 
      ctaaattat tagagataa tctgttaag acaaacagg 
      ttgtaaatt ttgtatttg tccgaatta agtaataaa 
      tctatcgaa ctgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY