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Gene information
General information
TC015782 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8060046..8064680
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015782
    iBeetle screen iB_05893

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015782 RA
      MNLVTTFTF LLLLSLNQI LADEKVRYD NFQVYRLTP 
      RSQKAVQAL RELEENGYD FWTSVRGVN QPVDVMVPP 
      HYKHRFNDF IRLNEFDAQ IYIPDVQKL IDNERPQTR 
      KSVGLEWTD YHTLDEIND WLKDLASTN DNIELVTAG 
      KTYEGRDIL GVKVSFKAG NENRTVFIE SNIHAREWI 
      SSAVTTWML NEILTSKDS EVRQVAESH DWYFVPVFN 
      PDGFVYTHT TDRLWRKTR VPYSSCYGA DPNRNWDYH 
      FNDGGASPS PCSETYCGP KAFSEPTTK SLSEFITTI 
      APKLQAYIA FHSYSQLLL LPYGYTSKH LENYQELYD 
      VGVKAATSL AKRYGTKYE VGNIVDVLY VASGGSMDW 
      VKGTFGTRI TYTYELRDT GRYGFVLPP DQIIPTAEE 
      TFDSLVTIL QEFDKS
    CDS sequence
    • >TC015782 RA
      atgaatctt gttacgact tttactttc cttcttctc 
      ctctctctt aaccaaatc cttgccgac gagaaagtt 
      cgttacgac aacttccaa gtctaccga ttgactcct 
      cgatcgcaa aaagccgtc caagcttta cgagaactt 
      gaagaaaat ggttatgac ttctggaca agtgttaga 
      ggagtcaac caaccggtt gatgtcatg gttcctcct 
      cattacaag caccgattc aatgatttc atccgtttg 
      aatgaattc gatgcccaa atttatatc ccagatgtt 
      caaaaactg attgataac gaaagacct caaacaaga 
      aagtctgta ggtctcgaa tggactgat tatcacact 
      ttggatgag atcaacgat tggttgaaa gaccttgct 
      tcaaccaat gacaacatc gaattggtc acagctggg 
      aagacgtac gaaggacga gatattttg ggtgtgaag 
      gtctctttc aaagcaggg aatgagaat aggactgtt 
      tttatcgaa tcaaatatt catgcacgc gaatggata 
      agttcggct gtgacaacc tggatgttg aacgaaatc 
      ctgaccagc aaagattcc gaagttcgc caagttgcc 
      gagagtcat gattggtac tttgtcccc gttttcaat 
      ccagacgga ttcgtttac actcacact acggaccgc 
      ttgtggcgt aaaacacgc gtgccatat tcctcatgt 
      tatggcgct gatcctaac aggaattgg gattatcat 
      tttaatgac ggtggagca agtccttcc ccttgctct 
      gaaacctac tgtggacct aaggccttc tcagaaccg 
      accactaag agcctgtct gaatttatc acaacaata 
      gctccgaaa ttgcaagct tacattgcc ttccattcg 
      tattcgcaa cttttgctg ctgccttat ggatacacg 
      tccaaacat ttggaaaat tatcaagaa ttgtacgat 
      gttggagtg aaagctgcc acgagtttg gccaagcga 
      tacggcacg aaatacgaa gttggaaac attgtcgat 
      gttctttat gtggcaagt ggaggcagt atggactgg 
      gtaaaaggt acttttgga acccgaatt acctacact 
      tatgaattg agagataca ggacgttat ggatttgtg 
      ttgccacct gatcagatt attccaaca gctgaagaa 
      acctttgat tctcttgta acaatattg caagagttc 
      gataaatcg taa
    mRNA sequence
    • >TC015782 RA
      ataaataaa acaccgcat tcaactctt caactagtc 
      tcttaaaag tgccaaaaa atgaatctt gttacgact 
      tttactttc cttcttctc ctctctctt aaccaaatc 
      cttgccgac gagaaagtt cgttacgac aacttccaa 
      gtctaccga ttgactcct cgatcgcaa aaagccgtc 
      caagcttta cgagaactt gaagaaaat ggttatgac 
      ttctggaca agtgttaga ggagtcaac caaccggtt 
      gatgtcatg gttcctcct cattacaag caccgattc 
      aatgatttc atccgtttg aatgaattc gatgcccaa 
      atttatatc ccagatgtt caaaaactg attgataac 
      gaaagacct caaacaaga aagtctgta ggtctcgaa 
      tggactgat tatcacact ttggatgag atcaacgat 
      tggttgaaa gaccttgct tcaaccaat gacaacatc 
      gaattggtc acagctggg aagacgtac gaaggacga 
      gatattttg ggtgtgaag gtctctttc aaagcaggg 
      aatgagaat aggactgtt tttatcgaa tcaaatatt 
      catgcacgc gaatggata agttcggct gtgacaacc 
      tggatgttg aacgaaatc ctgaccagc aaagattcc 
      gaagttcgc caagttgcc gagagtcat gattggtac 
      tttgtcccc gttttcaat ccagacgga ttcgtttac 
      actcacact acggaccgc ttgtggcgt aaaacacgc 
      gtgccatat tcctcatgt tatggcgct gatcctaac 
      aggaattgg gattatcat tttaatgac ggtggagca 
      agtccttcc ccttgctct gaaacctac tgtggacct 
      aaggccttc tcagaaccg accactaag agcctgtct 
      gaatttatc acaacaata gctccgaaa ttgcaagct 
      tacattgcc ttccattcg tattcgcaa cttttgctg 
      ctgccttat ggatacacg tccaaacat ttggaaaat 
      tatcaagaa ttgtacgat gttggagtg aaagctgcc 
      acgagtttg gccaagcga tacggcacg aaatacgaa 
      gttggaaac attgtcgat gttctttat gtggcaagt 
      ggaggcagt atggactgg gtaaaaggt acttttgga 
      acccgaatt acctacact tatgaattg agagataca 
      ggacgttat ggatttgtg ttgccacct gatcagatt 
      attccaaca gctgaagaa acctttgat tctcttgta 
      acaatattg caagagttc gataaatcg taaacaaaa 
      atgtaacac tagaaataa aataaaaat actgaaaat 
      ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY