| General information | |||
| TC015760 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 7583403..7591799 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015760 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05892 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015760 RA
MDKSAGKSH KSRGQTRTM GATYFDPDP VLEQSRKDQ TSPHKPKPR VGPSDDSDI RLQPSLTTR VDLLTHDTS VPPGTKYKP PKTRKQRKA DKQRREKYV QDLRFCTYD VTQMPSPTK ITKVDVKLK APSAEIKEA KVFPRKPSV SPSDIHPEV PTPAELAER QPKSGTIIN IKHQRPKKR KRKPRQEPE PFETPQGPT IEELIAQEL ATIQEIGEG GEEKLEPPQ PPPENYLTR QFRRKVSQM SKTTSIQSK DWHLEEGEG EDQVVEHED RFINTLVQK FRRESEIKR YERPRRKIK PGTFENFSF TKLRQPTLK TFYYGVKLG TVHQGDYHF SHLSRNKQG ICMPIAAYC FSILKHPDK WTQADIDDV LEAGNDLFL ESADTQHLH GDNVELKIT DLRKYCKLG KRKVRFVVS EPEISGMVR SDDKKIYNL TKAINIFFS RYSAGILQT EHLNIALWR DKHYYFFDG GARTIDLFS HPNGVALMA NFYDTASII TVLLNRSSL ENVPFIIYS INAFKVLLR DAEEVDSTG VIPESDNFQ VLDEEKAVA LGSFDLADK CFGFSRNKQ ALTIAIIAL VYARISPPS SWHKGTVDK IVIIGNQLF LECIENDSV EEVKIEDLP AVFTIGPYL VEVYVVPNT FVDFLFKKS KCVLLERLE KFFETASNA IVQIDNCAL AVWKQRNIY HCFDPYSRN NEGLKCREG TACVSMHTT LDSLIKTIA TNFDHKDMI FHVHAMKVC KIHRDTTTK DLFPQCLTM NEYPIENFK KIKMRKGHR KATNKSVTV DQTTRATFL GEIPEESIL EVGSNIRSI ALEELPSMV HQVPSKPLP KVTPPDQEF VADLDSPSL SDTQIEPPP PEEPEGEPS IEFVDLDGF ELTQEEKEM VERGEGEKG KGKGRTKAR RSEAEEMGE EAGEGEGGE GAQEWYMAA SEFTQIGSQ SFVSRVSQE VNTELFPIE VPQEEVPSL VRDIKALAT RRRNDIQAM YEFEMGQEP EVTMSEELA KETNFVNLP DGSQIILGG TNMAAFGSE LEFMAPFVC IMASAVAKK YSIDSWSRD VVDYVLKCG AELHQNSNT RYDQNFKLE IPRISLGKT DFSIRVNYI FDTYLKPRV LAMAINNML FPQWNSGLV VTPTYSCAI FYKNHLYYL YDGFGNNEV GLSKGVSNE GVACLARFL DINALVARI IHNKAKREQ EENVEYNRF VLSSCYVKQ LPREEVEEV EVEEQAEGG DQDEPVVVE SVKKKKKEP EEKKPVKMG YHLVDKLYK IEGTRSLGG GGTVSDTLK EDYFVCLCA CLLLLNLPI KRWDDRKID TVLDQGIHI FSHVEDHDV CSKKFIKNI LIDDYIFDI IANRVKFAT YSSGRTFRS GIETMVNKK HEHFILQFP DRCYALSVQ DKIYHLFDP NDSSGKASW TRYSNFGKL IKRIRKNLA KGGESYNFH CFEIMTINK APKSVIVSN RVKKYKIPK GKKPEIVGK PFYEEISWL EVDPIPWSW RFNKSGLPL WNHWFIDFP NDLFSLFGC LSPLDERFP QELPKKQTL GNLVAAIGM CHIYDLEDW NPTIMDSIL VFGDNYFQE CVKDITDEN YEVTFEDLK KECTIFPYS FQVVFTPAV EGTLFLVRL KQFNLFKAL RAFFDDYIK RFGIICVSK GDNDRRFYA FGKIQESEY FLYDCETLG PPMFIEKDS GVPYILRAT SFNRLLHVM TLTLRGGDF YIFEVTLSG FKAVA
- >TC015760 RA
atggataaa tcggccggt aagagccac aaaagtcgg gggcagacc cgcacaatg ggggccact tatttcgac cccgaccct gtcctggag caaagccgc aaagaccag accagcccc cataaaccc aaacctcga gttgggcct tcggacgac tcagacata agactccaa ccatcgcta acgacaaga gtcgatctt ttgacacac gatacgagc gtaccgcct ggtactaaa tacaaaccg cccaaaaca cgaaaacag aggaaagcc gacaagcaa aggcgggaa aagtacgtc caagacttg cgtttttgc acttatgat gtcacacaa atgccaagt cccaccaaa atcacgaaa gtcgatgtt aagctgaag gcaccgtca gccgaaatc aaggaagcc aaagttttt ccacgtaaa ccttcagtc tcgcctagt gatatccac ccagaggtg ccaacacca gccgaattg gccgaacgc cagcccaaa agtggtacc atcatcaat atcaagcac caaagaccc aaaaaaagg aaacggaag ccgcgacaa gaacccgaa ccttttgaa actccccaa ggacccacg attgaggaa cttatagcg caggaattg gccactatc caggaaatt ggcgaagga ggggaggag aaattggaa ccaccacaa ccaccacct gagaactac ctcaccagg caattccga cgtaaagtc tcccaaatg tccaaaacc acctcaatc cagagcaaa gactggcac ttggaggag ggagaaggt gaagatcag gtggtggag cacgaagac cgttttata aacacacta gtgcaaaaa tttcggcgc gaatccgaa atcaaacgc tacgaacga ccacgacgt aaaatcaag ccaggaacc ttcgagaac ttttccttc accaaactg agacaacca acgttgaaa accttctat tacggagtc aaactaggt acagtccac caaggcgac taccatttc tcccacttg agccgcaac aaacaaggc atttgtatg ccaatagct gcgtattgt ttctcaatc ttgaaacac ccagacaag tggacacaa gccgacatt gacgacgtg ctggaagcc ggaaacgac ctcttcctg gaaagtgcc gacacccag cacttgcac ggcgacaac gtcgagctg aaaatcacc gatttgcgc aaatactgc aagttgggg aaacgtaaa gtgcggttc gtggtcagc gagccggaa atttcaggc atggtgcgg tcagacgac aagaaaatc tacaacttg accaaagcc atcaatatt ttcttttcg cgctactcg gctggaatt ctacaaacc gagcacttg aacattgcg ctttggcgc gacaaacac tactatttc ttcgacggt ggtgccaga actattgac ttgttctcg cacccgaat ggtgtggcc ctcatggct aatttttac gacactgcg tccataatc accgttttg ttgaaccgc tcaagtctt gagaacgta ccttttatt atttactca atcaatgcg tttaaagta ctgctgcgg gacgccgaa gaagttgac tcgactggc gttattcca gagagtgac aatttccag gttctggat gaggagaag gcagtggcg ttgggttcg tttgatttg gcagacaag tgttttggt ttctcgcgc aataaacag gcactgacc attgcaatc attgcgctg gtttacgcc agaatctca ccgccgagt tcctggcac aagggaacc gttgacaag attgtgatc attgggaac caactgttt ctggaatgt atcgagaat gattcggtt gaggaggtg aaaattgag gatttgccg gcggttttt acaataggg ccgtacttg gtcgaagtc tatgtcgta ccaaatact ttcgttgat ttccttttt aagaaaagc aagtgtgtt ttgttggaa cgtttggag aagttcttc gagactgcg tccaatgcc atcgtccag attgacaat tgtgcctta gccgtttgg aaacagaga aacatctac cattgtttt gacccttat tcccgcaac aatgagggt ttgaaatgc cgtgaaggc acagcttgt gtgtcaatg cacacgact ttggattcg ttgataaaa acaatagct acgaatttc gaccataag gacatgatc ttccatgtg catgccatg aaggtgtgc aagatccac cgcgacacc accaccaaa gacctgttc ccgcagtgt ttgaccatg aacgagtac ccgatcgaa aatttcaaa aaaatcaaa atgaggaaa gggcaccga aaagccaca aataaatcg gtaacagtt gaccaaaca acacgagct acgtttctt ggcgaaatc cccgaagaa tcaatcctg gaggtgggt tccaacatc cggagcatc gcccttgag gagttacct tcaatggtg caccaagta ccatcgaag ccgcttcca aaggttacg cccccagac caggagttt gtggcggat ttggattcg ccgagtctt tccgacacg cagatcgaa cccccacca ccggaggaa ccagaaggt gaaccctcg attgagttt gttgacttg gatgggttc gagttgacc caggaggag aaggagatg gtggaacgt ggtgaaggt gagaaaggg aaaggtaaa gggaggacg aaggcgcga cgatcggag gcggaggaa atgggtgag gaagctggt gaaggtgaa ggtggggaa ggtgcccaa gagtggtac atggcagcg tccgaattc acccaaatc gggtcacaa tcgttcgtt agtcgcgta tcacaagaa gtcaacact gagttgttc cccattgaa gtaccacaa gaggaggta ccatctcta gtgcgcgat attaaggcg ctggcgacg aggcgtcgg aacgacatt caagccatg tacgagttt gagatggga caggaaccc gaagttaca atgtcggag gagttggcc aaagaaacg aactttgtt aatttacca gatggtagc caaatcatc cttggtggt accaacatg gcggcgttt gggtcagaa cttgagttt atggcacct tttgtctgt atcatggcc tcagcagtg gccaagaaa tactcaatt gattcttgg tcgcgtgat gtcgttgat tatgtcctt aaatgtggg gcggaattg caccaaaac tccaacacg aggtacgac caaaatttc aagctcgaa attccgagg atcagtttg gggaaaacc gattttagt atcagggtt aattatatt ttcgatacg tatctaaag ccaagagtt ctggcaatg gctatcaac aatatgctg ttcccgcag tggaattca ggtcttgtg gtcacaccg acttactcc tgtgctatt ttttataaa aatcatctc tattatctt tacgacggg tttgggaac aatgaggtg ggtttgagt aaaggtgtt tcgaatgaa ggagttgcg tgtcttgca cgttttttg gatattaat gcgttggtg gcgaggatt attcataat aaggcgaaa cgggagcaa gaggagaat gttgagtat aatcggttt gttttgagt tcgtgttat gtcaagcaa ttgccgagg gaagaagtt gaggaagtg gaagttgag gaacaggcg gaagggggg gatcaggat gagcctgtt gttgtggag agtgttaag aagaagaag aaggaaccg gaggagaaa aagccggtc aagatgggg taccacttg gtggacaaa ttgtacaaa atcgagggc acccgtagc cttggaggc ggcggcacc gtctccgac accctcaaa gaggactat ttcgtctgc ctctgtgcc tgcctcctc ctcctcaac ctcccaatc aaacgctgg gacgaccgc aaaatcgac acagtcctc gaccaaggc atccacatc ttcagccat gtggaagac cacgacgta tgttcgaaa aaattcatc aaaaacatt ctcatcgac gactacatt ttcgacatc atcgcaaat cgagtcaaa tttgcaacc tactcctca gggcgcact ttccgttca ggcattgaa acgatggta aacaaaaaa cacgaacac tttattttg caatttccc gaccggtgc tacgcccta agtgtccaa gacaagatc taccatttg ttcgacccc aacgactcc tcaggcaaa gcctcctgg accagatac agcaatttc gggaagttg atcaaacgg atcaggaaa aatttggca aaaggtggc gagagctac aatttccac tgtttcgag atcatgacg attaataag gcccccaag agcgtgatt gtgagcaac agggtgaag aaatataaa attccaaaa ggaaagaaa ccggaaatt gtcggtaaa ccattctat gaggaaatt tcctggttg gaagttgac ccgattccg tggtcttgg cgcttcaat aagagtggg ttaccgttg tggaaccac tggtttatc gactttccg aacgatttg ttctcgttg tttggctgt ttgagtccg ttggacgag aggttcccg caggaattg cccaaaaag caaacccta gggaacttg gttgctgca atagggatg tgccatatt tatgatctg gaggattgg aaccccact atcatggac tcgattcta gttttcggc gataattac tttcaagag tgtgttaaa gatattaca gatgagaac tacgaagtc acttttgag gatttgaag aaggagtgt acgattttc ccttacagt tttcaagtg gtgttcact ccagctgtc gaaggaacg ctttttctt gtgcgcttg aaacagttc aacttgttt aaggccttg agggcgttt tttgacgat tatatcaaa cggtttgga atcatttgt gtttcgaag ggtgataat gatagaagg ttttacgct tttgggaaa attcaagaa tcagagtac tttttgtac gattgtgaa actttaggg cctccaatg ttcattgag aaggactca ggggtgccc tatattttg cgagcgact tctttcaac agacttttg cacgtcatg actttgact ttgagaggc ggcgatttt tacatcttt gaagtgact ttatctgga tttaaggca gttgcttaa
- >TC015760 RA
cgattatac caaaaaatt ttgattgat tgaattttg acagccaaa atttcagtt atggataaa tcggccggt aagagccac aaaagtcgg gggcagacc cgcacaatg ggggccact tatttcgac cccgaccct gtcctggag caaagccgc aaagaccag accagcccc cataaaccc aaacctcga gttgggcct tcggacgac tcagacata agactccaa ccatcgcta acgacaaga gtcgatctt ttgacacac gatacgagc gtaccgcct ggtactaaa tacaaaccg cccaaaaca cgaaaacag aggaaagcc gacaagcaa aggcgggaa aagtacgtc caagacttg cgtttttgc acttatgat gtcacacaa atgccaagt cccaccaaa atcacgaaa gtcgatgtt aagctgaag gcaccgtca gccgaaatc aaggaagcc aaagttttt ccacgtaaa ccttcagtc tcgcctagt gatatccac ccagaggtg ccaacacca gccgaattg gccgaacgc cagcccaaa agtggtacc atcatcaat atcaagcac caaagaccc aaaaaaagg aaacggaag ccgcgacaa gaacccgaa ccttttgaa actccccaa ggacccacg attgaggaa cttatagcg caggaattg gccactatc caggaaatt ggcgaagga ggggaggag aaattggaa ccaccacaa ccaccacct gagaactac ctcaccagg caattccga cgtaaagtc tcccaaatg tccaaaacc acctcaatc cagagcaaa gactggcac ttggaggag ggagaaggt gaagatcag gtggtggag cacgaagac cgttttata aacacacta gtgcaaaaa tttcggcgc gaatccgaa atcaaacgc tacgaacga ccacgacgt aaaatcaag ccaggaacc ttcgagaac ttttccttc accaaactg agacaacca acgttgaaa accttctat tacggagtc aaactaggt acagtccac caaggcgac taccatttc tcccacttg agccgcaac aaacaaggc atttgtatg ccaatagct gcgtattgt ttctcaatc ttgaaacac ccagacaag tggacacaa gccgacatt gacgacgtg ctggaagcc ggaaacgac ctcttcctg gaaagtgcc gacacccag cacttgcac ggcgacaac gtcgagctg aaaatcacc gatttgcgc aaatactgc aagttgggg aaacgtaaa gtgcggttc gtggtcagc gagccggaa atttcaggc atggtgcgg tcagacgac aagaaaatc tacaacttg accaaagcc atcaatatt ttcttttcg cgctactcg gctggaatt ctacaaacc gagcacttg aacattgcg ctttggcgc gacaaacac tactatttc ttcgacggt ggtgccaga actattgac ttgttctcg cacccgaat ggtgtggcc ctcatggct aatttttac gacactgcg tccataatc accgttttg ttgaaccgc tcaagtctt gagaacgta ccttttatt atttactca atcaatgcg tttaaagta ctgctgcgg gacgccgaa gaagttgac tcgactggc gttattcca gagagtgac aatttccag gttctggat gaggagaag gcagtggcg ttgggttcg tttgatttg gcagacaag tgttttggt ttctcgcgc aataaacag gcactgacc attgcaatc attgcgctg gtttacgcc agaatctca ccgccgagt tcctggcac aagggaacc gttgacaag attgtgatc attgggaac caactgttt ctggaatgt atcgagaat gattcggtt gaggaggtg aaaattgag gatttgccg gcggttttt acaataggg ccgtacttg gtcgaagtc tatgtcgta ccaaatact ttcgttgat ttccttttt aagaaaagc aagtgtgtt ttgttggaa cgtttggag aagttcttc gagactgcg tccaatgcc atcgtccag attgacaat tgtgcctta gccgtttgg aaacagaga aacatctac cattgtttt gacccttat tcccgcaac aatgagggt ttgaaatgc cgtgaaggc acagcttgt gtgtcaatg cacacgact ttggattcg ttgataaaa acaatagct acgaatttc gaccataag gacatgatc ttccatgtg catgccatg aaggtgtgc aagatccac cgcgacacc accaccaaa gacctgttc ccgcagtgt ttgaccatg aacgagtac ccgatcgaa aatttcaaa aaaatcaaa atgaggaaa gggcaccga aaagccaca aataaatcg gtaacagtt gaccaaaca acacgagct acgtttctt ggcgaaatc cccgaagaa tcaatcctg gaggtgggt tccaacatc cggagcatc gcccttgag gagttacct tcaatggtg caccaagta ccatcgaag ccgcttcca aaggttacg cccccagac caggagttt gtggcggat ttggattcg ccgagtctt tccgacacg cagatcgaa cccccacca ccggaggaa ccagaaggt gaaccctcg attgagttt gttgacttg gatgggttc gagttgacc caggaggag aaggagatg gtggaacgt ggtgaaggt gagaaaggg aaaggtaaa gggaggacg aaggcgcga cgatcggag gcggaggaa atgggtgag gaagctggt gaaggtgaa ggtggggaa ggtgcccaa gagtggtac atggcagcg tccgaattc acccaaatc gggtcacaa tcgttcgtt agtcgcgta tcacaagaa gtcaacact gagttgttc cccattgaa gtaccacaa gaggaggta ccatctcta gtgcgcgat attaaggcg ctggcgacg aggcgtcgg aacgacatt caagccatg tacgagttt gagatggga caggaaccc gaagttaca atgtcggag gagttggcc aaagaaacg aactttgtt aatttacca gatggtagc caaatcatc cttggtggt accaacatg gcggcgttt gggtcagaa cttgagttt atggcacct tttgtctgt atcatggcc tcagcagtg gccaagaaa tactcaatt gattcttgg tcgcgtgat gtcgttgat tatgtcctt aaatgtggg gcggaattg caccaaaac tccaacacg aggtacgac caaaatttc aagctcgaa attccgagg atcagtttg gggaaaacc gattttagt atcagggtt aattatatt ttcgatacg tatctaaag ccaagagtt ctggcaatg gctatcaac aatatgctg ttcccgcag tggaattca ggtcttgtg gtcacaccg acttactcc tgtgctatt ttttataaa aatcatctc tattatctt tacgacggg tttgggaac aatgaggtg ggtttgagt aaaggtgtt tcgaatgaa ggagttgcg tgtcttgca cgttttttg gatattaat gcgttggtg gcgaggatt attcataat aaggcgaaa cgggagcaa gaggagaat gttgagtat aatcggttt gttttgagt tcgtgttat gtcaagcaa ttgccgagg gaagaagtt gaggaagtg gaagttgag gaacaggcg gaagggggg gatcaggat gagcctgtt gttgtggag agtgttaag aagaagaag aaggaaccg gaggagaaa aagccggtc aagatgggg taccacttg gtggacaaa ttgtacaaa atcgagggc acccgtagc cttggaggc ggcggcacc gtctccgac accctcaaa gaggactat ttcgtctgc ctctgtgcc tgcctcctc ctcctcaac ctcccaatc aaacgctgg gacgaccgc aaaatcgac acagtcctc gaccaaggc atccacatc ttcagccat gtggaagac cacgacgta tgttcgaaa aaattcatc aaaaacatt ctcatcgac gactacatt ttcgacatc atcgcaaat cgagtcaaa tttgcaacc tactcctca gggcgcact ttccgttca ggcattgaa acgatggta aacaaaaaa cacgaacac tttattttg caatttccc gaccggtgc tacgcccta agtgtccaa gacaagatc taccatttg ttcgacccc aacgactcc tcaggcaaa gcctcctgg accagatac agcaatttc gggaagttg atcaaacgg atcaggaaa aatttggca aaaggtggc gagagctac aatttccac tgtttcgag atcatgacg attaataag gcccccaag agcgtgatt gtgagcaac agggtgaag aaatataaa attccaaaa ggaaagaaa ccggaaatt gtcggtaaa ccattctat gaggaaatt tcctggttg gaagttgac ccgattccg tggtcttgg cgcttcaat aagagtggg ttaccgttg tggaaccac tggtttatc gactttccg aacgatttg ttctcgttg tttggctgt ttgagtccg ttggacgag aggttcccg caggaattg cccaaaaag caaacccta gggaacttg gttgctgca atagggatg tgccatatt tatgatctg gaggattgg aaccccact atcatggac tcgattcta gttttcggc gataattac tttcaagag tgtgttaaa gatattaca gatgagaac tacgaagtc acttttgag gatttgaag aaggagtgt acgattttc ccttacagt tttcaagtg gtgttcact ccagctgtc gaaggaacg ctttttctt gtgcgcttg aaacagttc aacttgttt aaggccttg agggcgttt tttgacgat tatatcaaa cggtttgga atcatttgt gtttcgaag ggtgataat gatagaagg ttttacgct tttgggaaa attcaagaa tcagagtac tttttgtac gattgtgaa actttaggg cctccaatg ttcattgag aaggactca ggggtgccc tatattttg cgagcgact tctttcaac agacttttg cacgtcatg actttgact ttgagaggc ggcgatttt tacatcttt gaagtgact ttatctgga tttaaggca gttgcttaa ggcactttt catttggtg ttgtttggt gtaagaaat aaaacactt tcgattta
