Status
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Gene information
General information
TC015760 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7583403..7591799
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015760
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05892

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015760 RA
      MDKSAGKSH KSRGQTRTM GATYFDPDP VLEQSRKDQ 
      TSPHKPKPR VGPSDDSDI RLQPSLTTR VDLLTHDTS 
      VPPGTKYKP PKTRKQRKA DKQRREKYV QDLRFCTYD 
      VTQMPSPTK ITKVDVKLK APSAEIKEA KVFPRKPSV 
      SPSDIHPEV PTPAELAER QPKSGTIIN IKHQRPKKR 
      KRKPRQEPE PFETPQGPT IEELIAQEL ATIQEIGEG 
      GEEKLEPPQ PPPENYLTR QFRRKVSQM SKTTSIQSK 
      DWHLEEGEG EDQVVEHED RFINTLVQK FRRESEIKR 
      YERPRRKIK PGTFENFSF TKLRQPTLK TFYYGVKLG 
      TVHQGDYHF SHLSRNKQG ICMPIAAYC FSILKHPDK 
      WTQADIDDV LEAGNDLFL ESADTQHLH GDNVELKIT 
      DLRKYCKLG KRKVRFVVS EPEISGMVR SDDKKIYNL 
      TKAINIFFS RYSAGILQT EHLNIALWR DKHYYFFDG 
      GARTIDLFS HPNGVALMA NFYDTASII TVLLNRSSL 
      ENVPFIIYS INAFKVLLR DAEEVDSTG VIPESDNFQ 
      VLDEEKAVA LGSFDLADK CFGFSRNKQ ALTIAIIAL 
      VYARISPPS SWHKGTVDK IVIIGNQLF LECIENDSV 
      EEVKIEDLP AVFTIGPYL VEVYVVPNT FVDFLFKKS 
      KCVLLERLE KFFETASNA IVQIDNCAL AVWKQRNIY 
      HCFDPYSRN NEGLKCREG TACVSMHTT LDSLIKTIA 
      TNFDHKDMI FHVHAMKVC KIHRDTTTK DLFPQCLTM 
      NEYPIENFK KIKMRKGHR KATNKSVTV DQTTRATFL 
      GEIPEESIL EVGSNIRSI ALEELPSMV HQVPSKPLP 
      KVTPPDQEF VADLDSPSL SDTQIEPPP PEEPEGEPS 
      IEFVDLDGF ELTQEEKEM VERGEGEKG KGKGRTKAR 
      RSEAEEMGE EAGEGEGGE GAQEWYMAA SEFTQIGSQ 
      SFVSRVSQE VNTELFPIE VPQEEVPSL VRDIKALAT 
      RRRNDIQAM YEFEMGQEP EVTMSEELA KETNFVNLP 
      DGSQIILGG TNMAAFGSE LEFMAPFVC IMASAVAKK 
      YSIDSWSRD VVDYVLKCG AELHQNSNT RYDQNFKLE 
      IPRISLGKT DFSIRVNYI FDTYLKPRV LAMAINNML 
      FPQWNSGLV VTPTYSCAI FYKNHLYYL YDGFGNNEV 
      GLSKGVSNE GVACLARFL DINALVARI IHNKAKREQ 
      EENVEYNRF VLSSCYVKQ LPREEVEEV EVEEQAEGG 
      DQDEPVVVE SVKKKKKEP EEKKPVKMG YHLVDKLYK 
      IEGTRSLGG GGTVSDTLK EDYFVCLCA CLLLLNLPI 
      KRWDDRKID TVLDQGIHI FSHVEDHDV CSKKFIKNI 
      LIDDYIFDI IANRVKFAT YSSGRTFRS GIETMVNKK 
      HEHFILQFP DRCYALSVQ DKIYHLFDP NDSSGKASW 
      TRYSNFGKL IKRIRKNLA KGGESYNFH CFEIMTINK 
      APKSVIVSN RVKKYKIPK GKKPEIVGK PFYEEISWL 
      EVDPIPWSW RFNKSGLPL WNHWFIDFP NDLFSLFGC 
      LSPLDERFP QELPKKQTL GNLVAAIGM CHIYDLEDW 
      NPTIMDSIL VFGDNYFQE CVKDITDEN YEVTFEDLK 
      KECTIFPYS FQVVFTPAV EGTLFLVRL KQFNLFKAL 
      RAFFDDYIK RFGIICVSK GDNDRRFYA FGKIQESEY 
      FLYDCETLG PPMFIEKDS GVPYILRAT SFNRLLHVM 
      TLTLRGGDF YIFEVTLSG FKAVA
    CDS sequence
    • >TC015760 RA
      atggataaa tcggccggt aagagccac aaaagtcgg 
      gggcagacc cgcacaatg ggggccact tatttcgac 
      cccgaccct gtcctggag caaagccgc aaagaccag 
      accagcccc cataaaccc aaacctcga gttgggcct 
      tcggacgac tcagacata agactccaa ccatcgcta 
      acgacaaga gtcgatctt ttgacacac gatacgagc 
      gtaccgcct ggtactaaa tacaaaccg cccaaaaca 
      cgaaaacag aggaaagcc gacaagcaa aggcgggaa 
      aagtacgtc caagacttg cgtttttgc acttatgat 
      gtcacacaa atgccaagt cccaccaaa atcacgaaa 
      gtcgatgtt aagctgaag gcaccgtca gccgaaatc 
      aaggaagcc aaagttttt ccacgtaaa ccttcagtc 
      tcgcctagt gatatccac ccagaggtg ccaacacca 
      gccgaattg gccgaacgc cagcccaaa agtggtacc 
      atcatcaat atcaagcac caaagaccc aaaaaaagg 
      aaacggaag ccgcgacaa gaacccgaa ccttttgaa 
      actccccaa ggacccacg attgaggaa cttatagcg 
      caggaattg gccactatc caggaaatt ggcgaagga 
      ggggaggag aaattggaa ccaccacaa ccaccacct 
      gagaactac ctcaccagg caattccga cgtaaagtc 
      tcccaaatg tccaaaacc acctcaatc cagagcaaa 
      gactggcac ttggaggag ggagaaggt gaagatcag 
      gtggtggag cacgaagac cgttttata aacacacta 
      gtgcaaaaa tttcggcgc gaatccgaa atcaaacgc 
      tacgaacga ccacgacgt aaaatcaag ccaggaacc 
      ttcgagaac ttttccttc accaaactg agacaacca 
      acgttgaaa accttctat tacggagtc aaactaggt 
      acagtccac caaggcgac taccatttc tcccacttg 
      agccgcaac aaacaaggc atttgtatg ccaatagct 
      gcgtattgt ttctcaatc ttgaaacac ccagacaag 
      tggacacaa gccgacatt gacgacgtg ctggaagcc 
      ggaaacgac ctcttcctg gaaagtgcc gacacccag 
      cacttgcac ggcgacaac gtcgagctg aaaatcacc 
      gatttgcgc aaatactgc aagttgggg aaacgtaaa 
      gtgcggttc gtggtcagc gagccggaa atttcaggc 
      atggtgcgg tcagacgac aagaaaatc tacaacttg 
      accaaagcc atcaatatt ttcttttcg cgctactcg 
      gctggaatt ctacaaacc gagcacttg aacattgcg 
      ctttggcgc gacaaacac tactatttc ttcgacggt 
      ggtgccaga actattgac ttgttctcg cacccgaat 
      ggtgtggcc ctcatggct aatttttac gacactgcg 
      tccataatc accgttttg ttgaaccgc tcaagtctt 
      gagaacgta ccttttatt atttactca atcaatgcg 
      tttaaagta ctgctgcgg gacgccgaa gaagttgac 
      tcgactggc gttattcca gagagtgac aatttccag 
      gttctggat gaggagaag gcagtggcg ttgggttcg 
      tttgatttg gcagacaag tgttttggt ttctcgcgc 
      aataaacag gcactgacc attgcaatc attgcgctg 
      gtttacgcc agaatctca ccgccgagt tcctggcac 
      aagggaacc gttgacaag attgtgatc attgggaac 
      caactgttt ctggaatgt atcgagaat gattcggtt 
      gaggaggtg aaaattgag gatttgccg gcggttttt 
      acaataggg ccgtacttg gtcgaagtc tatgtcgta 
      ccaaatact ttcgttgat ttccttttt aagaaaagc 
      aagtgtgtt ttgttggaa cgtttggag aagttcttc 
      gagactgcg tccaatgcc atcgtccag attgacaat 
      tgtgcctta gccgtttgg aaacagaga aacatctac 
      cattgtttt gacccttat tcccgcaac aatgagggt 
      ttgaaatgc cgtgaaggc acagcttgt gtgtcaatg 
      cacacgact ttggattcg ttgataaaa acaatagct 
      acgaatttc gaccataag gacatgatc ttccatgtg 
      catgccatg aaggtgtgc aagatccac cgcgacacc 
      accaccaaa gacctgttc ccgcagtgt ttgaccatg 
      aacgagtac ccgatcgaa aatttcaaa aaaatcaaa 
      atgaggaaa gggcaccga aaagccaca aataaatcg 
      gtaacagtt gaccaaaca acacgagct acgtttctt 
      ggcgaaatc cccgaagaa tcaatcctg gaggtgggt 
      tccaacatc cggagcatc gcccttgag gagttacct 
      tcaatggtg caccaagta ccatcgaag ccgcttcca 
      aaggttacg cccccagac caggagttt gtggcggat 
      ttggattcg ccgagtctt tccgacacg cagatcgaa 
      cccccacca ccggaggaa ccagaaggt gaaccctcg 
      attgagttt gttgacttg gatgggttc gagttgacc 
      caggaggag aaggagatg gtggaacgt ggtgaaggt 
      gagaaaggg aaaggtaaa gggaggacg aaggcgcga 
      cgatcggag gcggaggaa atgggtgag gaagctggt 
      gaaggtgaa ggtggggaa ggtgcccaa gagtggtac 
      atggcagcg tccgaattc acccaaatc gggtcacaa 
      tcgttcgtt agtcgcgta tcacaagaa gtcaacact 
      gagttgttc cccattgaa gtaccacaa gaggaggta 
      ccatctcta gtgcgcgat attaaggcg ctggcgacg 
      aggcgtcgg aacgacatt caagccatg tacgagttt 
      gagatggga caggaaccc gaagttaca atgtcggag 
      gagttggcc aaagaaacg aactttgtt aatttacca 
      gatggtagc caaatcatc cttggtggt accaacatg 
      gcggcgttt gggtcagaa cttgagttt atggcacct 
      tttgtctgt atcatggcc tcagcagtg gccaagaaa 
      tactcaatt gattcttgg tcgcgtgat gtcgttgat 
      tatgtcctt aaatgtggg gcggaattg caccaaaac 
      tccaacacg aggtacgac caaaatttc aagctcgaa 
      attccgagg atcagtttg gggaaaacc gattttagt 
      atcagggtt aattatatt ttcgatacg tatctaaag 
      ccaagagtt ctggcaatg gctatcaac aatatgctg 
      ttcccgcag tggaattca ggtcttgtg gtcacaccg 
      acttactcc tgtgctatt ttttataaa aatcatctc 
      tattatctt tacgacggg tttgggaac aatgaggtg 
      ggtttgagt aaaggtgtt tcgaatgaa ggagttgcg 
      tgtcttgca cgttttttg gatattaat gcgttggtg 
      gcgaggatt attcataat aaggcgaaa cgggagcaa 
      gaggagaat gttgagtat aatcggttt gttttgagt 
      tcgtgttat gtcaagcaa ttgccgagg gaagaagtt 
      gaggaagtg gaagttgag gaacaggcg gaagggggg 
      gatcaggat gagcctgtt gttgtggag agtgttaag 
      aagaagaag aaggaaccg gaggagaaa aagccggtc 
      aagatgggg taccacttg gtggacaaa ttgtacaaa 
      atcgagggc acccgtagc cttggaggc ggcggcacc 
      gtctccgac accctcaaa gaggactat ttcgtctgc 
      ctctgtgcc tgcctcctc ctcctcaac ctcccaatc 
      aaacgctgg gacgaccgc aaaatcgac acagtcctc 
      gaccaaggc atccacatc ttcagccat gtggaagac 
      cacgacgta tgttcgaaa aaattcatc aaaaacatt 
      ctcatcgac gactacatt ttcgacatc atcgcaaat 
      cgagtcaaa tttgcaacc tactcctca gggcgcact 
      ttccgttca ggcattgaa acgatggta aacaaaaaa 
      cacgaacac tttattttg caatttccc gaccggtgc 
      tacgcccta agtgtccaa gacaagatc taccatttg 
      ttcgacccc aacgactcc tcaggcaaa gcctcctgg 
      accagatac agcaatttc gggaagttg atcaaacgg 
      atcaggaaa aatttggca aaaggtggc gagagctac 
      aatttccac tgtttcgag atcatgacg attaataag 
      gcccccaag agcgtgatt gtgagcaac agggtgaag 
      aaatataaa attccaaaa ggaaagaaa ccggaaatt 
      gtcggtaaa ccattctat gaggaaatt tcctggttg 
      gaagttgac ccgattccg tggtcttgg cgcttcaat 
      aagagtggg ttaccgttg tggaaccac tggtttatc 
      gactttccg aacgatttg ttctcgttg tttggctgt 
      ttgagtccg ttggacgag aggttcccg caggaattg 
      cccaaaaag caaacccta gggaacttg gttgctgca 
      atagggatg tgccatatt tatgatctg gaggattgg 
      aaccccact atcatggac tcgattcta gttttcggc 
      gataattac tttcaagag tgtgttaaa gatattaca 
      gatgagaac tacgaagtc acttttgag gatttgaag 
      aaggagtgt acgattttc ccttacagt tttcaagtg 
      gtgttcact ccagctgtc gaaggaacg ctttttctt 
      gtgcgcttg aaacagttc aacttgttt aaggccttg 
      agggcgttt tttgacgat tatatcaaa cggtttgga 
      atcatttgt gtttcgaag ggtgataat gatagaagg 
      ttttacgct tttgggaaa attcaagaa tcagagtac 
      tttttgtac gattgtgaa actttaggg cctccaatg 
      ttcattgag aaggactca ggggtgccc tatattttg 
      cgagcgact tctttcaac agacttttg cacgtcatg 
      actttgact ttgagaggc ggcgatttt tacatcttt 
      gaagtgact ttatctgga tttaaggca gttgcttaa
    mRNA sequence
    • >TC015760 RA
      cgattatac caaaaaatt ttgattgat tgaattttg 
      acagccaaa atttcagtt atggataaa tcggccggt 
      aagagccac aaaagtcgg gggcagacc cgcacaatg 
      ggggccact tatttcgac cccgaccct gtcctggag 
      caaagccgc aaagaccag accagcccc cataaaccc 
      aaacctcga gttgggcct tcggacgac tcagacata 
      agactccaa ccatcgcta acgacaaga gtcgatctt 
      ttgacacac gatacgagc gtaccgcct ggtactaaa 
      tacaaaccg cccaaaaca cgaaaacag aggaaagcc 
      gacaagcaa aggcgggaa aagtacgtc caagacttg 
      cgtttttgc acttatgat gtcacacaa atgccaagt 
      cccaccaaa atcacgaaa gtcgatgtt aagctgaag 
      gcaccgtca gccgaaatc aaggaagcc aaagttttt 
      ccacgtaaa ccttcagtc tcgcctagt gatatccac 
      ccagaggtg ccaacacca gccgaattg gccgaacgc 
      cagcccaaa agtggtacc atcatcaat atcaagcac 
      caaagaccc aaaaaaagg aaacggaag ccgcgacaa 
      gaacccgaa ccttttgaa actccccaa ggacccacg 
      attgaggaa cttatagcg caggaattg gccactatc 
      caggaaatt ggcgaagga ggggaggag aaattggaa 
      ccaccacaa ccaccacct gagaactac ctcaccagg 
      caattccga cgtaaagtc tcccaaatg tccaaaacc 
      acctcaatc cagagcaaa gactggcac ttggaggag 
      ggagaaggt gaagatcag gtggtggag cacgaagac 
      cgttttata aacacacta gtgcaaaaa tttcggcgc 
      gaatccgaa atcaaacgc tacgaacga ccacgacgt 
      aaaatcaag ccaggaacc ttcgagaac ttttccttc 
      accaaactg agacaacca acgttgaaa accttctat 
      tacggagtc aaactaggt acagtccac caaggcgac 
      taccatttc tcccacttg agccgcaac aaacaaggc 
      atttgtatg ccaatagct gcgtattgt ttctcaatc 
      ttgaaacac ccagacaag tggacacaa gccgacatt 
      gacgacgtg ctggaagcc ggaaacgac ctcttcctg 
      gaaagtgcc gacacccag cacttgcac ggcgacaac 
      gtcgagctg aaaatcacc gatttgcgc aaatactgc 
      aagttgggg aaacgtaaa gtgcggttc gtggtcagc 
      gagccggaa atttcaggc atggtgcgg tcagacgac 
      aagaaaatc tacaacttg accaaagcc atcaatatt 
      ttcttttcg cgctactcg gctggaatt ctacaaacc 
      gagcacttg aacattgcg ctttggcgc gacaaacac 
      tactatttc ttcgacggt ggtgccaga actattgac 
      ttgttctcg cacccgaat ggtgtggcc ctcatggct 
      aatttttac gacactgcg tccataatc accgttttg 
      ttgaaccgc tcaagtctt gagaacgta ccttttatt 
      atttactca atcaatgcg tttaaagta ctgctgcgg 
      gacgccgaa gaagttgac tcgactggc gttattcca 
      gagagtgac aatttccag gttctggat gaggagaag 
      gcagtggcg ttgggttcg tttgatttg gcagacaag 
      tgttttggt ttctcgcgc aataaacag gcactgacc 
      attgcaatc attgcgctg gtttacgcc agaatctca 
      ccgccgagt tcctggcac aagggaacc gttgacaag 
      attgtgatc attgggaac caactgttt ctggaatgt 
      atcgagaat gattcggtt gaggaggtg aaaattgag 
      gatttgccg gcggttttt acaataggg ccgtacttg 
      gtcgaagtc tatgtcgta ccaaatact ttcgttgat 
      ttccttttt aagaaaagc aagtgtgtt ttgttggaa 
      cgtttggag aagttcttc gagactgcg tccaatgcc 
      atcgtccag attgacaat tgtgcctta gccgtttgg 
      aaacagaga aacatctac cattgtttt gacccttat 
      tcccgcaac aatgagggt ttgaaatgc cgtgaaggc 
      acagcttgt gtgtcaatg cacacgact ttggattcg 
      ttgataaaa acaatagct acgaatttc gaccataag 
      gacatgatc ttccatgtg catgccatg aaggtgtgc 
      aagatccac cgcgacacc accaccaaa gacctgttc 
      ccgcagtgt ttgaccatg aacgagtac ccgatcgaa 
      aatttcaaa aaaatcaaa atgaggaaa gggcaccga 
      aaagccaca aataaatcg gtaacagtt gaccaaaca 
      acacgagct acgtttctt ggcgaaatc cccgaagaa 
      tcaatcctg gaggtgggt tccaacatc cggagcatc 
      gcccttgag gagttacct tcaatggtg caccaagta 
      ccatcgaag ccgcttcca aaggttacg cccccagac 
      caggagttt gtggcggat ttggattcg ccgagtctt 
      tccgacacg cagatcgaa cccccacca ccggaggaa 
      ccagaaggt gaaccctcg attgagttt gttgacttg 
      gatgggttc gagttgacc caggaggag aaggagatg 
      gtggaacgt ggtgaaggt gagaaaggg aaaggtaaa 
      gggaggacg aaggcgcga cgatcggag gcggaggaa 
      atgggtgag gaagctggt gaaggtgaa ggtggggaa 
      ggtgcccaa gagtggtac atggcagcg tccgaattc 
      acccaaatc gggtcacaa tcgttcgtt agtcgcgta 
      tcacaagaa gtcaacact gagttgttc cccattgaa 
      gtaccacaa gaggaggta ccatctcta gtgcgcgat 
      attaaggcg ctggcgacg aggcgtcgg aacgacatt 
      caagccatg tacgagttt gagatggga caggaaccc 
      gaagttaca atgtcggag gagttggcc aaagaaacg 
      aactttgtt aatttacca gatggtagc caaatcatc 
      cttggtggt accaacatg gcggcgttt gggtcagaa 
      cttgagttt atggcacct tttgtctgt atcatggcc 
      tcagcagtg gccaagaaa tactcaatt gattcttgg 
      tcgcgtgat gtcgttgat tatgtcctt aaatgtggg 
      gcggaattg caccaaaac tccaacacg aggtacgac 
      caaaatttc aagctcgaa attccgagg atcagtttg 
      gggaaaacc gattttagt atcagggtt aattatatt 
      ttcgatacg tatctaaag ccaagagtt ctggcaatg 
      gctatcaac aatatgctg ttcccgcag tggaattca 
      ggtcttgtg gtcacaccg acttactcc tgtgctatt 
      ttttataaa aatcatctc tattatctt tacgacggg 
      tttgggaac aatgaggtg ggtttgagt aaaggtgtt 
      tcgaatgaa ggagttgcg tgtcttgca cgttttttg 
      gatattaat gcgttggtg gcgaggatt attcataat 
      aaggcgaaa cgggagcaa gaggagaat gttgagtat 
      aatcggttt gttttgagt tcgtgttat gtcaagcaa 
      ttgccgagg gaagaagtt gaggaagtg gaagttgag 
      gaacaggcg gaagggggg gatcaggat gagcctgtt 
      gttgtggag agtgttaag aagaagaag aaggaaccg 
      gaggagaaa aagccggtc aagatgggg taccacttg 
      gtggacaaa ttgtacaaa atcgagggc acccgtagc 
      cttggaggc ggcggcacc gtctccgac accctcaaa 
      gaggactat ttcgtctgc ctctgtgcc tgcctcctc 
      ctcctcaac ctcccaatc aaacgctgg gacgaccgc 
      aaaatcgac acagtcctc gaccaaggc atccacatc 
      ttcagccat gtggaagac cacgacgta tgttcgaaa 
      aaattcatc aaaaacatt ctcatcgac gactacatt 
      ttcgacatc atcgcaaat cgagtcaaa tttgcaacc 
      tactcctca gggcgcact ttccgttca ggcattgaa 
      acgatggta aacaaaaaa cacgaacac tttattttg 
      caatttccc gaccggtgc tacgcccta agtgtccaa 
      gacaagatc taccatttg ttcgacccc aacgactcc 
      tcaggcaaa gcctcctgg accagatac agcaatttc 
      gggaagttg atcaaacgg atcaggaaa aatttggca 
      aaaggtggc gagagctac aatttccac tgtttcgag 
      atcatgacg attaataag gcccccaag agcgtgatt 
      gtgagcaac agggtgaag aaatataaa attccaaaa 
      ggaaagaaa ccggaaatt gtcggtaaa ccattctat 
      gaggaaatt tcctggttg gaagttgac ccgattccg 
      tggtcttgg cgcttcaat aagagtggg ttaccgttg 
      tggaaccac tggtttatc gactttccg aacgatttg 
      ttctcgttg tttggctgt ttgagtccg ttggacgag 
      aggttcccg caggaattg cccaaaaag caaacccta 
      gggaacttg gttgctgca atagggatg tgccatatt 
      tatgatctg gaggattgg aaccccact atcatggac 
      tcgattcta gttttcggc gataattac tttcaagag 
      tgtgttaaa gatattaca gatgagaac tacgaagtc 
      acttttgag gatttgaag aaggagtgt acgattttc 
      ccttacagt tttcaagtg gtgttcact ccagctgtc 
      gaaggaacg ctttttctt gtgcgcttg aaacagttc 
      aacttgttt aaggccttg agggcgttt tttgacgat 
      tatatcaaa cggtttgga atcatttgt gtttcgaag 
      ggtgataat gatagaagg ttttacgct tttgggaaa 
      attcaagaa tcagagtac tttttgtac gattgtgaa 
      actttaggg cctccaatg ttcattgag aaggactca 
      ggggtgccc tatattttg cgagcgact tctttcaac 
      agacttttg cacgtcatg actttgact ttgagaggc 
      ggcgatttt tacatcttt gaagtgact ttatctgga 
      tttaaggca gttgcttaa ggcactttt catttggtg 
      ttgtttggt gtaagaaat aaaacactt tcgattta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY