Status
Please wait ...
Gene information
General information
TC030912
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015758,TC015759
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7495099..7503452
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030912
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05890

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_05891

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030912 RA
      MDDLKHKIL GLINKQGPK IPSMGFSDA NYHFPQVEN 
      LSINSKTVA ETNRPSDQE ILESIEAAY FSIRDDFDI 
      CRFELGKLP QTLDCDQIQ RDFKTLKQQ HQVVSKKVL 
      QLILEHQSG CNTEFDKVL EILEQVKDT LQECKTSRR 
      HLKVTADQF RASLELLAN YRKRKIAQN LLNNLKMIK 
      YLRCYDQRL QGLLRDEDY AGAIIELKK CHKIATKYR 
      HFSCVAALT YKLQETLES TDGQLDRVL AQTCYHFDG 
      ERYQKLLLA YHLLDKSEI SMIDNLHVH YITAIYNTS 
      LNVVHSYVT SAESIESSD TSGKNPYKT LCQSVGSDV 
      FIPCLVSLC KVLFKIVLS YHQLINWHN NQEQETNLN 
      KEKLDHNLT KIWDDVQRK VSGLLLNAD LASYKFDQF 
      VQVLGVVHR LIQVGEEFC LSKSDDLQE SIRKQSVNY 
      FKNYHIQRL EELKIFLEN EMWEICPVK PTFTLLQLQ 
      EFKSLRGVL RNFKLRPPA TGGAYSTAS TDCCSSNHS 
      QDGSSIVGN YFVRYAESG TPFDSGLDE TVIEEDILA 
      GENDGSGYF SEESDEEPE ELRRDFVDE ENASPKKRE 
      KQINCLVLT NTTLSVLRQ MGKYLQMST R
    • >TC030912 RB
      MSVGAQAFD LRQILVSMA KINWEVKEV MSQHNTYID 
      RILREVQIF TLRLEEVAH KVPVSVEVS TSLWESIAH 
      IITHTLVQG FSEAKKCSN GGRALMQLD FIQFLSKFE 
      KMAGLRPVP HREYVENYI KAFYLPESE LEKWIKEHG 
      EYSAKHLFG LVSCACQSN KKTKQRLLQ VIDEGDRGD 
      R
    • >TC030912 RC
      MDDLKHKIL GLINKQGPK IPSMGFSDA NYHFPQVEN 
      LSINSKTVA ETNRPSDQE ILESIEAAY FSIRDDFDI 
      CRFELGKLP QTLDCDQIQ RDFKTLKQQ HQVVSKKVL 
      QLILEHQSG CNTEFDKVL EILEQVKDT LQECKTSRR 
      HLKVTADQF RASLELLAN YRKRKIAQN LLNNLKMIK 
      YLRCYDQRL QGLLRDEDY AGAIIELKK CHKIATKYR 
      HFSCVAALT YKLQETLES TDGQLDRVL AQTCYHFDG 
      ERYQKLLLA YHLLDKSEI SMIDNLHVH YITAIYNTS 
      LNVVHSYVT SAESIESSD TSGKNPYKT LCQSVGSDV 
      FIPCLVSLC KVLFKIVLS YHQLINWHN NQEQETNLN 
      KEKLDHNLT KIWDDVQRK VSGLLLNAD LASYKFDQF 
      VQVLGVVHR LIQVGEEFC LSKSDDLQE SIRKQSVNY 
      FKNYHIQRL EELKIFLEN EMWEICPVK PTFTLLQLQ 
      EFKSLRGVL RNFKLRPPA TGGAYSTAS TDCCSSNHS 
      QDGSSIVGN YFVRYAESG TPFDSGLDE TVIEEDILA 
      GENDGSGYF SEESDEEPE ELRRDFVDE ENASPKKRE 
      KQINCLVLT NTTLSVLRQ MGNCMNQLF DFYLYSVHA 
      FFAADLTVA SNSLYTPEL TQTLKKISD NLVTEFEEP 
      PTDETPPKI LKPALSPMV DLTKPEHLH GLAERIVGV 
      ESVIFLAKQ YEYLQEFLE YLIPPTNKI MLQQFFTQN 
      ISSAKDLRK PVYMSVGAQ AFDLRQILV SMAKINWEV 
      KEVMSQHNT YIDRILREV QIFTLRLEE VAHKVPVSV 
      EVSTSLWES IAHIITHTL VQGFSEAKK CSNGGRALM 
      QLDFIQFLS KFEKMAGLR PVPHREYVE NYIKAFYLP 
      ESELEKWIK EHGEYSAKH LFGLVSCAC QSNKKTKQR 
      LLQVIDEGD RGDR
    CDS sequence
    • >TC030912 RA
      atggacgac ttgaagcac aaaattctg ggcctgatc 
      aacaaacag gggcctaaa atcccgtcg atggggttc 
      tccgacgcc aattatcac ttcccccaa gtggagaac 
      ctatcaatt aatagtaag acagttgct gagacaaat 
      aggccctca gaccaggag attttggaa agtattgaa 
      gcggcctat ttttccatc cgggacgac tttgacatt 
      tgccgattt gaactgggg aagttgccc caaacgctc 
      gactgtgac cagatccag cgcgacttc aaaaccctc 
      aaacagcaa caccaagtg gtctcgaag aaagtcctc 
      caattaata ttggagcac caaagtggc tgtaacacc 
      gagttcgat aaagtctta gagattttg gagcaagtt 
      aaggacaca cttcaggag tgtaaaact agtcggaga 
      cacttaaaa gtgaccgcg gaccagttc cgcgcctcc 
      cttgagctc ctcgccaac taccgtaaa cgcaaaatt 
      gcgcaaaac ctgctaaac aacctcaaa atgatcaaa 
      tatctccgg tgttatgac caacgattg caagggctt 
      ttacgcgat gaggactac gcgggggcg atcattgaa 
      ctcaaaaag tgccacaaa atcgccacc aagtatcga 
      catttcagc tgcgtggcc gccctaact tataaattg 
      caagaaact ttggagagt acggatggg caacttgac 
      cgggttttg gcacagacg tgttatcat tttgatggt 
      gagcgctat cagaaatta ctgcttgct tatcattta 
      ttggataag tctgaaatt tcaatgatt gataatttg 
      cacgtccat tatatcact gcgatctac aatacgtcg 
      ttaaatgtg gtgcattcg tacgtcacg tcggccgaa 
      agtatcgaa agttcggac acgagtggg aaaaatccg 
      tacaaaacg ctttgccaa agtgttggg agcgacgtt 
      tttattcca tgtctggtg agtttgtgc aaagtcttg 
      ttcaagatc gtgctaagt taccaccaa ttgatcaat 
      tggcataac aaccaagag caagagaca aatttaaat 
      aaggaaaaa ctggaccac aatttgact aaaatctgg 
      gatgacgtg caacgcaaa gtctcaggg cttctcctc 
      aacgctgat ttggcctct tataagttc gaccagttc 
      gtccaagtt ttgggtgtt gtgcaccgc ttgatccaa 
      gttggcgag gaattttgt ctgagcaaa tcggacgat 
      ttgcaagaa tcaattcgt aaacaaagc gtcaactat 
      ttcaaaaat taccacatc cagcgtttg gaggaatta 
      aaaatcttt ctcgagaat gaaatgtgg gagatttgt 
      cccgttaag cccaccttt actctcctc caattgcaa 
      gagtttaag tctttaagg ggggttctt aggaatttt 
      aagttgcgt ccgccggcc acaggtggc gcttattcg 
      acggcttcg actgattgt tgttcgtcg aaccattcg 
      caagatggg agtagtatt gttggcaat tattttgtg 
      agatatgca gaaagtgga acgccgttt gattcagga 
      cttgatgaa actgtaatc gaagaggat attcttgca 
      ggggaaaat gacgggtct ggttatttt tcggaagaa 
      agtgatgaa gaaccggaa gagttgagg agggatttt 
      gttgatgag gaaaatgca agtcccaag aaacgggaa 
      aaacagatc aattgtttg gttttgacc aatacaacg 
      ttgtcggtt ttgagacaa atggggaag tatttgcaa 
      atgtcgacc cgctga
    • >TC030912 RB
      atgtccgtg ggggcgcaa gcgttcgat ttgcgtcaa 
      attttggtc tcaatggcg aaaattaac tgggaggtg 
      aaggaagtc atgtcgcaa cacaacacc tacattgac 
      cgaatcctc cgagaggtg caaattttc acattgcgg 
      ttggaggaa gtcgctcac aaagtcccc gtttctgtt 
      gaagtttcc acttcgttg tgggaaagt atcgcccat 
      ataatcacc cacacgttg gttcaaggg ttcagcgag 
      gcgaagaag tgttcaaac ggggggcgg gctctaatg 
      caacttgat tttatccaa tttttgtcc aagtttgaa 
      aaaatggct ggtttaagg cccgttcct catcgcgaa 
      tatgtggaa aactatata aaggcgttt tatttgccc 
      gaatctgag ttggaaaag tggattaag gaacatggg 
      gaatattcg gctaaacat ttgttcggg ttggtttcc 
      tgtgcgtgt cagagtaat aagaagacg aaacagagg 
      cttttgcaa gtgattgat gagggggac aggggggat 
      aggtag
    • >TC030912 RC
      atggacgac ttgaagcac aaaattctg ggcctgatc 
      aacaaacag gggcctaaa atcccgtcg atggggttc 
      tccgacgcc aattatcac ttcccccaa gtggagaac 
      ctatcaatt aatagtaag acagttgct gagacaaat 
      aggccctca gaccaggag attttggaa agtattgaa 
      gcggcctat ttttccatc cgggacgac tttgacatt 
      tgccgattt gaactgggg aagttgccc caaacgctc 
      gactgtgac cagatccag cgcgacttc aaaaccctc 
      aaacagcaa caccaagtg gtctcgaag aaagtcctc 
      caattaata ttggagcac caaagtggc tgtaacacc 
      gagttcgat aaagtctta gagattttg gagcaagtt 
      aaggacaca cttcaggag tgtaaaact agtcggaga 
      cacttaaaa gtgaccgcg gaccagttc cgcgcctcc 
      cttgagctc ctcgccaac taccgtaaa cgcaaaatt 
      gcgcaaaac ctgctaaac aacctcaaa atgatcaaa 
      tatctccgg tgttatgac caacgattg caagggctt 
      ttacgcgat gaggactac gcgggggcg atcattgaa 
      ctcaaaaag tgccacaaa atcgccacc aagtatcga 
      catttcagc tgcgtggcc gccctaact tataaattg 
      caagaaact ttggagagt acggatggg caacttgac 
      cgggttttg gcacagacg tgttatcat tttgatggt 
      gagcgctat cagaaatta ctgcttgct tatcattta 
      ttggataag tctgaaatt tcaatgatt gataatttg 
      cacgtccat tatatcact gcgatctac aatacgtcg 
      ttaaatgtg gtgcattcg tacgtcacg tcggccgaa 
      agtatcgaa agttcggac acgagtggg aaaaatccg 
      tacaaaacg ctttgccaa agtgttggg agcgacgtt 
      tttattcca tgtctggtg agtttgtgc aaagtcttg 
      ttcaagatc gtgctaagt taccaccaa ttgatcaat 
      tggcataac aaccaagag caagagaca aatttaaat 
      aaggaaaaa ctggaccac aatttgact aaaatctgg 
      gatgacgtg caacgcaaa gtctcaggg cttctcctc 
      aacgctgat ttggcctct tataagttc gaccagttc 
      gtccaagtt ttgggtgtt gtgcaccgc ttgatccaa 
      gttggcgag gaattttgt ctgagcaaa tcggacgat 
      ttgcaagaa tcaattcgt aaacaaagc gtcaactat 
      ttcaaaaat taccacatc cagcgtttg gaggaatta 
      aaaatcttt ctcgagaat gaaatgtgg gagatttgt 
      cccgttaag cccaccttt actctcctc caattgcaa 
      gagtttaag tctttaagg ggggttctt aggaatttt 
      aagttgcgt ccgccggcc acaggtggc gcttattcg 
      acggcttcg actgattgt tgttcgtcg aaccattcg 
      caagatggg agtagtatt gttggcaat tattttgtg 
      agatatgca gaaagtgga acgccgttt gattcagga 
      cttgatgaa actgtaatc gaagaggat attcttgca 
      ggggaaaat gacgggtct ggttatttt tcggaagaa 
      agtgatgaa gaaccggaa gagttgagg agggatttt 
      gttgatgag gaaaatgca agtcccaag aaacgggaa 
      aaacagatc aattgtttg gttttgacc aatacaacg 
      ttgtcggtt ttgagacaa atggggaat tgcatgaac 
      cagttgttt gatttttat ctctattcg gttcatgcc 
      ttcttcgct gctgatttg accgtcgct agcaactct 
      ctctacacg cccgaactc acccaaacg ttgaaaaaa 
      atctccgac aatctagtc accgaattc gaggaaccg 
      cccactgac gaaaccccg cccaaaatc ctaaaaccc 
      gccctctca ccaatggtg gacctcacc aaacctgaa 
      cacttgcac ggattggct gaacgtatc gtgggcgtg 
      gaaagtgtc atctttctc gccaaacaa tacgaatat 
      ttacaggaa ttcctcgag tatttaatc ccgcccacg 
      aataaaata atgttgcaa cagtttttc acacagaac 
      atttcgagc gctaaggac ctacgaaag cccgtttac 
      atgtccgtg ggggcgcaa gcgttcgat ttgcgtcaa 
      attttggtc tcaatggcg aaaattaac tgggaggtg 
      aaggaagtc atgtcgcaa cacaacacc tacattgac 
      cgaatcctc cgagaggtg caaattttc acattgcgg 
      ttggaggaa gtcgctcac aaagtcccc gtttctgtt 
      gaagtttcc acttcgttg tgggaaagt atcgcccat 
      ataatcacc cacacgttg gttcaaggg ttcagcgag 
      gcgaagaag tgttcaaac ggggggcgg gctctaatg 
      caacttgat tttatccaa tttttgtcc aagtttgaa 
      aaaatggct ggtttaagg cccgttcct catcgcgaa 
      tatgtggaa aactatata aaggcgttt tatttgccc 
      gaatctgag ttggaaaag tggattaag gaacatggg 
      gaatattcg gctaaacat ttgttcggg ttggtttcc 
      tgtgcgtgt cagagtaat aagaagacg aaacagagg 
      cttttgcaa gtgattgat gagggggac aggggggat 
      aggtag
    mRNA sequence
    • >TC030912 RA
      ttgtcaaaa tgacaagtc agtagtatg tgtaaataa 
      ataaataaa taattaata tttcgttcg tcacaatat 
      cggttaata gtgttagga tggacgact tgaagcaca 
      aaattctgg gcctgatca acaaacagg ggcctaaaa 
      tcccgtcga tggggttct ccgacgcca attatcact 
      tcccccaag tggagaacc tatcaatta atagtaaga 
      cagttgctg agacaaata ggccctcag accaggaga 
      ttttggaaa gtattgaag cggcctatt tttccatcc 
      gggacgact ttgacattt gccgatttg aactgggga 
      agttgcccc aaacgctcg actgtgacc agatccagc 
      gcgacttca aaaccctca aacagcaac accaagtgg 
      tctcgaaga aagtcctcc aattaatat tggagcacc 
      aaagtggct gtaacaccg agttcgata aagtcttag 
      agattttgg agcaagtta aggacacac ttcaggagt 
      gtaaaacta gtcggagac acttaaaag tgaccgcgg 
      accagttcc gcgcctccc ttgagctcc tcgccaact 
      accgtaaac gcaaaattg cgcaaaacc tgctaaaca 
      acctcaaaa tgatcaaat atctccggt gttatgacc 
      aacgattgc aagggcttt tacgcgatg aggactacg 
      cgggggcga tcattgaac tcaaaaagt gccacaaaa 
      tcgccacca agtatcgac atttcagct gcgtggccg 
      ccctaactt ataaattgc aagaaactt tggagagta 
      cggatgggc aacttgacc gggttttgg cacagacgt 
      gttatcatt ttgatggtg agcgctatc agaaattac 
      tgcttgctt atcatttat tggataagt ctgaaattt 
      caatgattg ataatttgc acgtccatt atatcactg 
      cgatctaca atacgtcgt taaatgtgg tgcattcgt 
      acgtcacgt cggccgaaa gtatcgaaa gttcggaca 
      cgagtggga aaaatccgt acaaaacgc tttgccaaa 
      gtgttggga gcgacgttt ttattccat gtctggtga 
      gtttgtgca aagtcttgt tcaagatcg tgctaagtt 
      accaccaat tgatcaatt ggcataaca accaagagc 
      aagagacaa atttaaata aggaaaaac tggaccaca 
      atttgacta aaatctggg atgacgtgc aacgcaaag 
      tctcagggc ttctcctca acgctgatt tggcctctt 
      ataagttcg accagttcg tccaagttt tgggtgttg 
      tgcaccgct tgatccaag ttggcgagg aattttgtc 
      tgagcaaat cggacgatt tgcaagaat caattcgta 
      aacaaagcg tcaactatt tcaaaaatt accacatcc 
      agcgtttgg aggaattaa aaatctttc tcgagaatg 
      aaatgtggg agatttgtc ccgttaagc ccaccttta 
      ctctcctcc aattgcaag agtttaagt ctttaaggg 
      gggttctta ggaatttta agttgcgtc cgccggcca 
      caggtggcg cttattcga cggcttcga ctgattgtt 
      gttcgtcga accattcgc aagatggga gtagtattg 
      ttggcaatt attttgtga gatatgcag aaagtggaa 
      cgccgtttg attcaggac ttgatgaaa ctgtaatcg 
      aagaggata ttcttgcag gggaaaatg acgggtctg 
      gttattttt cggaagaaa gtgatgaag aaccggaag 
      agttgagga gggattttg ttgatgagg aaaatgcaa 
      gtcccaaga aacgggaaa aacagatca attgtttgg 
      ttttgacca atacaacgt tgtcggttt tgagacaaa 
      tggggaagt atttgcaaa tgtcgaccc gctgaaacc 
      gatagcgtt tcagattat cagttgcat gaaccagtt 
      gtttgattt ttatctcta ttcggttca tgccttctt 
      cgctgctga tttggtgag tttaagtat ttttgttgt 
      tggtaaaac agtttcctg tagtcgaaa aagaaacaa 
      ttgttcttg tt
    • >TC030912 RB
      tgaccgata aaatcccct agataattt aaaattaat 
      taaaaattt tagaacatt tcgagcgct aaggaccta 
      cgaaagccc gtttacatg tccgtgggg gcgcaagcg 
      ttcgatttg cgtcaaatt ttggtctca atggcgaaa 
      attaactgg gaggtgaag gaagtcatg tcgcaacac 
      aacacctac attgaccga atcctccga gaggtgcaa 
      attttcaca ttgcggttg gaggaagtc gctcacaaa 
      gtccccgtt tctgttgaa gtttccact tcgttgtgg 
      gaaagtatc gcccatata atcacccac acgttggtt 
      caagggttc agcgaggcg aagaagtgt tcaaacggg 
      gggcgggct ctaatgcaa cttgatttt atccaattt 
      ttgtccaag tttgaaaaa atggctggt ttaaggccc 
      gttcctcat cgcgaatat gtggaaaac tatataaag 
      gcgttttat ttgcccgaa tctgagttg gaaaagtgg 
      attaaggaa catggggaa tattcggct aaacatttg 
      ttcgggttg gtttcctgt gcgtgtcag agtaataag 
      aagacgaaa cagaggctt ttgcaagtg attgatgag 
      ggggacagg ggggatagg tagcctttg cttgattgt 
      gatatttga tagggcaca aagtgacat aaagtttca 
      attatttta tttagtttt ttacatgag caggcacta 
      tttatgtta acattttag gtttaatta ataattata 
      cacaaaatg aatttttta attacattg aattttgta 
      attcaataa tcaataaaa tttattaaa atcc
    • >TC030912 RC
      ttgtcaaaa tgacaagtc agtagtatg tgtaaataa 
      ataaataaa taattaata tttcgttcg tcacaatat 
      cggttaata gtgttagga tggacgact tgaagcaca 
      aaattctgg gcctgatca acaaacagg ggcctaaaa 
      tcccgtcga tggggttct ccgacgcca attatcact 
      tcccccaag tggagaacc tatcaatta atagtaaga 
      cagttgctg agacaaata ggccctcag accaggaga 
      ttttggaaa gtattgaag cggcctatt tttccatcc 
      gggacgact ttgacattt gccgatttg aactgggga 
      agttgcccc aaacgctcg actgtgacc agatccagc 
      gcgacttca aaaccctca aacagcaac accaagtgg 
      tctcgaaga aagtcctcc aattaatat tggagcacc 
      aaagtggct gtaacaccg agttcgata aagtcttag 
      agattttgg agcaagtta aggacacac ttcaggagt 
      gtaaaacta gtcggagac acttaaaag tgaccgcgg 
      accagttcc gcgcctccc ttgagctcc tcgccaact 
      accgtaaac gcaaaattg cgcaaaacc tgctaaaca 
      acctcaaaa tgatcaaat atctccggt gttatgacc 
      aacgattgc aagggcttt tacgcgatg aggactacg 
      cgggggcga tcattgaac tcaaaaagt gccacaaaa 
      tcgccacca agtatcgac atttcagct gcgtggccg 
      ccctaactt ataaattgc aagaaactt tggagagta 
      cggatgggc aacttgacc gggttttgg cacagacgt 
      gttatcatt ttgatggtg agcgctatc agaaattac 
      tgcttgctt atcatttat tggataagt ctgaaattt 
      caatgattg ataatttgc acgtccatt atatcactg 
      cgatctaca atacgtcgt taaatgtgg tgcattcgt 
      acgtcacgt cggccgaaa gtatcgaaa gttcggaca 
      cgagtggga aaaatccgt acaaaacgc tttgccaaa 
      gtgttggga gcgacgttt ttattccat gtctggtga 
      gtttgtgca aagtcttgt tcaagatcg tgctaagtt 
      accaccaat tgatcaatt ggcataaca accaagagc 
      aagagacaa atttaaata aggaaaaac tggaccaca 
      atttgacta aaatctggg atgacgtgc aacgcaaag 
      tctcagggc ttctcctca acgctgatt tggcctctt 
      ataagttcg accagttcg tccaagttt tgggtgttg 
      tgcaccgct tgatccaag ttggcgagg aattttgtc 
      tgagcaaat cggacgatt tgcaagaat caattcgta 
      aacaaagcg tcaactatt tcaaaaatt accacatcc 
      agcgtttgg aggaattaa aaatctttc tcgagaatg 
      aaatgtggg agatttgtc ccgttaagc ccaccttta 
      ctctcctcc aattgcaag agtttaagt ctttaaggg 
      gggttctta ggaatttta agttgcgtc cgccggcca 
      caggtggcg cttattcga cggcttcga ctgattgtt 
      gttcgtcga accattcgc aagatggga gtagtattg 
      ttggcaatt attttgtga gatatgcag aaagtggaa 
      cgccgtttg attcaggac ttgatgaaa ctgtaatcg 
      aagaggata ttcttgcag gggaaaatg acgggtctg 
      gttattttt cggaagaaa gtgatgaag aaccggaag 
      agttgagga gggattttg ttgatgagg aaaatgcaa 
      gtcccaaga aacgggaaa aacagatca attgtttgg 
      ttttgacca atacaacgt tgtcggttt tgagacaaa 
      tggggaatt gcatgaacc agttgtttg atttttatc 
      tctattcgg ttcatgcct tcttcgctg ctgatttga 
      ccgtcgcta gcaactctc tctacacgc ccgaactca 
      cccaaacgt tgaaaaaaa tctccgaca atctagtca 
      ccgaattcg aggaaccgc ccactgacg aaaccccgc 
      ccaaaatcc taaaacccg ccctctcac caatggtgg 
      acctcacca aacctgaac acttgcacg gattggctg 
      aacgtatcg tgggcgtgg aaagtgtca tctttctcg 
      ccaaacaat acgaatatt tacaggaat tcctcgagt 
      atttaatcc cgcccacga ataaaataa tgttgcaac 
      agtttttca cacagaaca tttcgagcg ctaaggacc 
      tacgaaagc ccgtttaca tgtccgtgg gggcgcaag 
      cgttcgatt tgcgtcaaa ttttggtct caatggcga 
      aaattaact gggaggtga aggaagtca tgtcgcaac 
      acaacacct acattgacc gaatcctcc gagaggtgc 
      aaattttca cattgcggt tggaggaag tcgctcaca 
      aagtccccg tttctgttg aagtttcca cttcgttgt 
      gggaaagta tcgcccata taatcaccc acacgttgg 
      ttcaagggt tcagcgagg cgaagaagt gttcaaacg 
      gggggcggg ctctaatgc aacttgatt ttatccaat 
      ttttgtcca agtttgaaa aaatggctg gtttaaggc 
      ccgttcctc atcgcgaat atgtggaaa actatataa 
      aggcgtttt atttgcccg aatctgagt tggaaaagt 
      ggattaagg aacatgggg aatattcgg ctaaacatt 
      tgttcgggt tggtttcct gtgcgtgtc agagtaata 
      agaagacga aacagaggc ttttgcaag tgattgatg 
      agggggaca ggggggata ggtagcctt tgcttgatt 
      gtgatattt gatagggca caaagtgac ataaagttt 
      caattattt tatttagtt ttttacatg agcaggcac 
      tatttatgt taacatttt aggtttaat taataatta 
      tacacaaaa tgaattttt taattacat tgaattttg 
      taattcaat aatcaataa aatttatta aaatcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY