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Gene information
General information
TC015746 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7362409..7364400
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015746
    iBeetle screen iB_05887

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015746 RA
      MCAILTILL ILSPNFVHC YQQWTTIYD KITQEEEFS 
      GYKTMIRND KASSLNLLY SGVTVFVPT NDAIRTYTG 
      FFRDAVVLY HMGTQPYTL KQLRLNNTV QSAHGDNPP 
      LWVSVTPDG YYINNAKIL EKRSNYMAK CKRNENLNQ 
      VLHVIDRVL DPMTPMANP SAFDFLQHY DQWDLGPFK 
      IFNFWTRVQ YHDKSEWYQ KPGGHTFFI PIDDKMDDY 
      RLQSIDSYV IDGHVVPNM VLFTRPTTK NFIFESASN 
      GDYIYIVLS MTSRNDRFY VKSFTVLGD SDHKRGEVF 
      AEIIKSDIP VTNGVVHLI SKPLAVFDR QLQFFPYLP 
      VIHKVTMDP NLDFTYNLA KNRINKILK RERGMYTYF 
      APKNQAWGN YTGYSRSQL VDILARHLI VSEVRYTIP 
      KLVALAKSG NGTTFLETL GGTLRLQID HFDGYYHLK 
      WGTKWVRVS RPDYRCSDG VVHVVDDVF ATPKFAKPP 
      QTPKSGFWR TLKDAFT
    CDS sequence
    • >TC015746 RA
      atgtgtgca atcctcacc attttgttg attctttcc 
      ccgaatttc gtccattgc taccaacaa tggacgacc 
      atctacgat aaaatcaca caagaggaa gagttctcc 
      ggatacaaa accatgata agaaacgat aaagcatcg 
      tctttgaat ttgttatac agcggtgtc actgttttc 
      gtgccaacg aacgacgca attcgaacc tatactggg 
      ttttttcgc gatgcggtc gttctgtac catatgggc 
      acccagcca tacactttg aaacaacta cggttgaat 
      aatacggta caaagcgcc catggggac aacccaccc 
      ctatgggtg agtgtcacc cctgatggg tactacatc 
      aacaacgcc aaaattttg gaaaaacgc tccaactac 
      atggcgaaa tgcaaaaga aacgagaac ttgaatcag 
      gtactacat gtgattgat cgggtactc gatcccatg 
      acacccatg gccaaccca tcagccttc gattttttg 
      caacactac gaccagtgg gatttaggg ccgttcaaa 
      atttttaac ttctggaca cgagtgcag taccatgac 
      aagtcagag tggtaccag aagccaggc ggtcacacg 
      tttttcatc ccaattgat gataaaatg gacgattac 
      cgcctccaa agcatcgat agttacgta attgacggg 
      catgtggta cccaacatg gtacttttc acacgccca 
      acaaccaaa aactttatt tttgaaagt gcctcaaat 
      ggggactac atttacatc gtactgtca atgacgtca 
      cgaaacgac cgtttctac gtcaaaagt ttcacagtt 
      ctgggcgac agtgaccat aaaagaggc gaagtcttt 
      gccgaaata atcaaaagt gatattccc gtaaccaac 
      ggagttgtc cacctaatt tccaaacct ttggctgtt 
      ttcgaccgc caactccag ttcttcccc tacttgcca 
      gtcatccac aaagtcaca atggacccg aacttggat 
      ttcacgtac aatttagcc aaaaacagg attaacaaa 
      atcctgaaa cgcgaacgg gggatgtac acgtacttc 
      gcccccaaa aaccaggct tgggggaac tacacgggg 
      tactcccgc agccaactt gtcgatatc cttgcccga 
      catttaatc gtgtctgaa gtacgctac acaatccct 
      aaactggtg gctttggct aaaagtggc aacggaact 
      acgtttctg gaaactttg ggtggtacc ctcagacta 
      caaatcgac cattttgat gggtactac catctcaaa 
      tggggcaca aaatgggtc agagtctcc cggcctgac 
      tacaggtgc agcgatggg gttgtccac gttgtcgac 
      gatgttttc gccaccccc aagtttgca aaaccgccc 
      caaacacct aaaagtggg ttttggagg acgttgaaa 
      gacgcgttc acgtga
    mRNA sequence
    • >TC015746 RA
      accttgccc caaaaataa tcaaaccga gatttcata 
      acgaaccaa gtgtgtgtc atgtgtgca atcctcacc 
      attttgttg attctttcc ccgaatttc gtccattgc 
      taccaacaa tggacgacc atctacgat aaaatcaca 
      caagaggaa gagttctcc ggatacaaa accatgata 
      agaaacgat aaagcatcg tctttgaat ttgttatac 
      agcggtgtc actgttttc gtgccaacg aacgacgca 
      attcgaacc tatactggg ttttttcgc gatgcggtc 
      gttctgtac catatgggc acccagcca tacactttg 
      aaacaacta cggttgaat aatacggta caaagcgcc 
      catggggac aacccaccc ctatgggtg agtgtcacc 
      cctgatggg tactacatc aacaacgcc aaaattttg 
      gaaaaacgc tccaactac atggcgaaa tgcaaaaga 
      aacgagaac ttgaatcag gtactacat gtgattgat 
      cgggtactc gatcccatg acacccatg gccaaccca 
      tcagccttc gattttttg caacactac gaccagtgg 
      gatttaggg ccgttcaaa atttttaac ttctggaca 
      cgagtgcag taccatgac aagtcagag tggtaccag 
      aagccaggc ggtcacacg tttttcatc ccaattgat 
      gataaaatg gacgattac cgcctccaa agcatcgat 
      agttacgta attgacggg catgtggta cccaacatg 
      gtacttttc acacgccca acaaccaaa aactttatt 
      tttgaaagt gcctcaaat ggggactac atttacatc 
      gtactgtca atgacgtca cgaaacgac cgtttctac 
      gtcaaaagt ttcacagtt ctgggcgac agtgaccat 
      aaaagaggc gaagtcttt gccgaaata atcaaaagt 
      gatattccc gtaaccaac ggagttgtc cacctaatt 
      tccaaacct ttggctgtt ttcgaccgc caactccag 
      ttcttcccc tacttgcca gtcatccac aaagtcaca 
      atggacccg aacttggat ttcacgtac aatttagcc 
      aaaaacagg attaacaaa atcctgaaa cgcgaacgg 
      gggatgtac acgtacttc gcccccaaa aaccaggct 
      tgggggaac tacacgggg tactcccgc agccaactt 
      gtcgatatc cttgcccga catttaatc gtgtctgaa 
      gtacgctac acaatccct aaactggtg gctttggct 
      aaaagtggc aacggaact acgtttctg gaaactttg 
      ggtggtacc ctcagacta caaatcgac cattttgat 
      gggtactac catctcaaa tggggcaca aaatgggtc 
      agagtctcc cggcctgac tacaggtgc agcgatggg 
      gttgtccac gttgtcgac gatgttttc gccaccccc 
      aagtttgca aaaccgccc caaacacct aaaagtggg 
      ttttggagg acgttgaaa gacgcgttc acgtgaccg 
      ccctttgat ttgttgcca gacatttgc ccgacttag 
      tgcccggtc atatttagt ggtaaaagt ttgttgttt 
      tatctacaa ttttcctag aaatacagg gtgattcaa 
      gtaagttgg ccaaaatcg cgctgaaat gtttaaact 
      aatattata ttcattttt gcataagta ggtttttat 
      aaaaaacta aatacctaa aaaattggt taaacttga 
      tcgaaaata attaaagta aaggcagtt ataaatctt 
      aatctgcgc tgactttat ttgagcttt ctgtttgaa 
      tctgatttg atttgaata actctgcat ctaatagta 
      cagcctagc cttaatttg gctccattt tattcgtct 
      aataaaact taaatctgg cataacaca gacaagtcg 
      tgaatttat ctaaataga taaactaaa atcttttga 
      tgaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY