Status
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Gene information
General information
TC015742 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7273763..7275611
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015742
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05886

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015742 RA
      MPVKRIANR FVHSCELSQ IDEKSKIHS PYDTCFQSF 
CHGLRKCLT IAKNVDSST YFRSLAAFN REKNRHLLS 
RKYRRVIHP FSNFARRED LVVSFLWFT IFVVQSYVG 
SFFNRELLD EKHAHVYRH VLAFANLLL LCDVVLRFF 
VGYLIDATR TVILDHKSI VKNYLKTYF IFDFGAGFS 
FLVFYIFNL DDRAQLILY ELHTLRIVR VRTILKNLD 
NLLCDFNVR ESIRIVVLI SFIGFLSLH TLTCAFGLI 
PVYRKVFQI QYEFENSWL SQTDNHSPQ TQFESGWEH 
THSSLQKYL HHLSMVLCH FVGCAIPKY KTADYLEML 
ILAWITIFG LIFYVYVVA KVLQLFGVV NISETKFEE 
LKIQSDKYM RRNDFPGDL RKRVNDYYD YKFDKKFFS 
EQKILDSLS EHLRMEVLL YSCRNLIEE VQIFKGLSR 
SAIGSVLAL LKQEVYVAN DVILEPDER TGDIYFILY 
GTCAIELIS GKEVMHIED GDYFGIVDM ETATDPSSI 
VSLEVSEVY KLERKDIKY CCKCFPEIK QKLEKMALH 
RMRKFNQMM SLRGVEDET TDDIIDNLR KGRILHRGI 
HRLAFL
    CDS sequence
    • >TC015742 RA
      atgccagta aaacgaatc gcaaatcgt ttcgtacac 
tcgtgtgag ctttcacaa atcgacgaa aagtccaaa 
atccacagc ccctacgac acctgcttc cagagcttc 
tgccacggt ctacgcaag tgcttgaca atagcgaaa 
aacgtcgac agttcaacc tacttccga agtcttgcc 
gccttcaat cgcgagaaa aaccgccac cttttatcc 
cgaaagtac cgacgcgtc atccatcct ttcagcaat 
ttcgcaaga cgagaagat ttagtcgtt tcattcctc 
tggtttacg attttcgtt gtgcaatcg tacgttggc 
agcttcttc aaccgagaa ctcctagac gaaaaacat 
gcccatgtg tatcgacat gtgctagct ttcgcaaac 
ttattgcta ctctgtgac gtggttttg cgctttttc 
gtgggctat ttgatcgac gccaccaga acggttatc 
ctggaccac aaatcgatc gtcaagaac tacctcaag 
acgtacttt atttttgat tttggtgcc ggttttagt 
tttttagtg ttctatatt tttaacctc gacgatcgc 
gcccaattg attctgtac gaactgcac actttgcgc 
atagtgaga gtgagaacg attctgaaa aacttggac 
aatcttttg tgcgatttc aacgtccgg gagtcgatc 
cgcattgtg gtcctgatc tccttcatc ggcttcctc 
agcttgcac actctgacc tgcgctttt ggcttgatc 
ccagtctac cggaaggtg ttccagatt caatacgag 
ttcgagaac tcctggtta tctcagacc gacaaccac 
tccccgcag acgcagttc gagtccggc tgggagcac 
actcacagt tccctccag aagtacctg caccacctc 
tccatggtt ctgtgccac ttcgtcggt tgtgcgatc 
ccaaagtac aagactgca gactacttg gaaatgctc 
atcctggcc tggatcact atcttcggt ctgattttc 
tacgtctat gtcgtcgcc aaagtgctc caactcttc 
ggcgtggtc aacatctcc gaaaccaag ttcgaagaa 
ttgaagatc cagtcggac aagtatatg cgaaggaac 
gactttccg ggggattta cgcaaacgc gtcaacgac 
tactatgac tacaagttc gacaagaag tttttctcc 
gaacagaaa atccttgat tcgctcagt gaacatctc 
cggatggaa gttcttctt tacagttgc cggaatcta 
atcgaagaa gtgcagatt ttcaaggga ttgtccagg 
tcagccatt ggctcggtt ctggcgctt ttgaagcag 
gaggtttac gtggcgaat gacgtgatt ttggaaccg 
gatgaacgg actggtgac atttatttt atactttac 
ggcacgtgt gcgattgaa ctaatctcg gggaaggaa 
gtgatgcat attgaagat ggggattat ttcgggatt 
gtcgatatg gaaacggca actgatcct tcgtctatt 
gtctcgctt gaagtgtcc gaagtttac aaactggag 
aggaaagat atcaagtat tgctgtaag tgctttcca 
gaaatcaag cagaaactg gagaagatg gctcttcac 
aggatgagg aagtttaac caaatgatg agtctgcga 
ggagttgag gacgagaca accgatgac attattgat 
aatttgagg aaagggaga atcctacat cggggaata 
catagacta gctttccta taa
    mRNA sequence
    • >TC015742 RA
      acggctgtc aaaatgcca gtaaaacga atcgcaaat 
cgtttcgta cactcgtgt gagctttca caaatcgac 
gaaaagtcc aaaatccac agcccctac gacacctgc 
ttccagagc ttctgccac ggtctacgc aagtgcttg 
acaatagcg aaaaacgtc gacagttca acctacttc 
cgaagtctt gccgccttc aatcgcgag aaaaaccgc 
cacctttta tcccgaaag taccgacgc gtcatccat 
cctttcagc aatttcgca agacgagaa gatttagtc 
gtttcattc ctctggttt acgattttc gttgtgcaa 
tcgtacgtt ggcagcttc ttcaaccga gaactccta 
gacgaaaaa catgcccat gtgtatcga catgtgcta 
gctttcgca aacttattg ctactctgt gacgtggtt 
ttgcgcttt ttcgtgggc tatttgatc gacgccacc 
agaacggtt atcctggac cacaaatcg atcgtcaag 
aactacctc aagacgtac tttattttt gattttggt 
gccggtttt agtttttta gtgttctat atttttaac 
ctcgacgat cgcgcccaa ttgattctg tacgaactg 
cacactttg cgcatagtg agagtgaga acgattctg 
aaaaacttg gacaatctt ttgtgcgat ttcaacgtc 
cgggagtcg atccgcatt gtggtcctg atctccttc 
atcggcttc ctcagcttg cacactctg acctgcgct 
tttggcttg atcccagtc taccggaag gtgttccag 
attcaatac gagttcgag aactcctgg ttatctcag 
accgacaac cactccccg cagacgcag ttcgagtcc 
ggctgggag cacactcac agttccctc cagaagtac 
ctgcaccac ctctccatg gttctgtgc cacttcgtc 
ggttgtgcg atcccaaag tacaagact gcagactac 
ttggaaatg ctcatcctg gcctggatc actatcttc 
ggtctgatt ttctacgtc tatgtcgtc gccaaagtg 
ctccaactc ttcggcgtg gtcaacatc tccgaaacc 
aagttcgaa gaattgaag atccagtcg gacaagtat 
atgcgaagg aacgacttt ccgggggat ttacgcaaa 
cgcgtcaac gactactat gactacaag ttcgacaag 
aagtttttc tccgaacag aaaatcctt gattcgctc 
agtgaacat ctccggatg gaagttctt ctttacagt 
tgccggaat ctaatcgaa gaagtgcag attttcaag 
ggattgtcc aggtcagcc attggctcg gttctggcg 
cttttgaag caggaggtt tacgtggcg aatgacgtg 
attttggaa ccggatgaa cggactggt gacatttat 
tttatactt tacggcacg tgtgcgatt gaactaatc 
tcggggaag gaagtgatg catattgaa gatggggat 
tatttcggg attgtcgat atggaaacg gcaactgat 
ccttcgtct attgtctcg cttgaagtg tccgaagtt 
tacaaactg gagaggaaa gatatcaag tattgctgt 
aagtgcttt ccagaaatc aagcagaaa ctggagaag 
atggctctt cacaggatg aggaagttt aaccaaatg 
atgagtctg cgaggagtt gaggacgag acaaccgat 
gacattatt gataatttg aggaaaggg agaatccta 
catcgggga atacataga ctagctttc ctataagtt 
tgtagattt ttaagcaat aaaaatgac caagttcca 
acttttttt tctacaagg aggcataaa ccaataaaa 
acttcccta aaac

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY