| General information | |||
| TC015742 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 7273763..7275611 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC015742 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05886 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC015742 RA
MPVKRIANR FVHSCELSQ IDEKSKIHS PYDTCFQSF CHGLRKCLT IAKNVDSST YFRSLAAFN REKNRHLLS RKYRRVIHP FSNFARRED LVVSFLWFT IFVVQSYVG SFFNRELLD EKHAHVYRH VLAFANLLL LCDVVLRFF VGYLIDATR TVILDHKSI VKNYLKTYF IFDFGAGFS FLVFYIFNL DDRAQLILY ELHTLRIVR VRTILKNLD NLLCDFNVR ESIRIVVLI SFIGFLSLH TLTCAFGLI PVYRKVFQI QYEFENSWL SQTDNHSPQ TQFESGWEH THSSLQKYL HHLSMVLCH FVGCAIPKY KTADYLEML ILAWITIFG LIFYVYVVA KVLQLFGVV NISETKFEE LKIQSDKYM RRNDFPGDL RKRVNDYYD YKFDKKFFS EQKILDSLS EHLRMEVLL YSCRNLIEE VQIFKGLSR SAIGSVLAL LKQEVYVAN DVILEPDER TGDIYFILY GTCAIELIS GKEVMHIED GDYFGIVDM ETATDPSSI VSLEVSEVY KLERKDIKY CCKCFPEIK QKLEKMALH RMRKFNQMM SLRGVEDET TDDIIDNLR KGRILHRGI HRLAFL
- >TC015742 RA
atgccagta aaacgaatc gcaaatcgt ttcgtacac tcgtgtgag ctttcacaa atcgacgaa aagtccaaa atccacagc ccctacgac acctgcttc cagagcttc tgccacggt ctacgcaag tgcttgaca atagcgaaa aacgtcgac agttcaacc tacttccga agtcttgcc gccttcaat cgcgagaaa aaccgccac cttttatcc cgaaagtac cgacgcgtc atccatcct ttcagcaat ttcgcaaga cgagaagat ttagtcgtt tcattcctc tggtttacg attttcgtt gtgcaatcg tacgttggc agcttcttc aaccgagaa ctcctagac gaaaaacat gcccatgtg tatcgacat gtgctagct ttcgcaaac ttattgcta ctctgtgac gtggttttg cgctttttc gtgggctat ttgatcgac gccaccaga acggttatc ctggaccac aaatcgatc gtcaagaac tacctcaag acgtacttt atttttgat tttggtgcc ggttttagt tttttagtg ttctatatt tttaacctc gacgatcgc gcccaattg attctgtac gaactgcac actttgcgc atagtgaga gtgagaacg attctgaaa aacttggac aatcttttg tgcgatttc aacgtccgg gagtcgatc cgcattgtg gtcctgatc tccttcatc ggcttcctc agcttgcac actctgacc tgcgctttt ggcttgatc ccagtctac cggaaggtg ttccagatt caatacgag ttcgagaac tcctggtta tctcagacc gacaaccac tccccgcag acgcagttc gagtccggc tgggagcac actcacagt tccctccag aagtacctg caccacctc tccatggtt ctgtgccac ttcgtcggt tgtgcgatc ccaaagtac aagactgca gactacttg gaaatgctc atcctggcc tggatcact atcttcggt ctgattttc tacgtctat gtcgtcgcc aaagtgctc caactcttc ggcgtggtc aacatctcc gaaaccaag ttcgaagaa ttgaagatc cagtcggac aagtatatg cgaaggaac gactttccg ggggattta cgcaaacgc gtcaacgac tactatgac tacaagttc gacaagaag tttttctcc gaacagaaa atccttgat tcgctcagt gaacatctc cggatggaa gttcttctt tacagttgc cggaatcta atcgaagaa gtgcagatt ttcaaggga ttgtccagg tcagccatt ggctcggtt ctggcgctt ttgaagcag gaggtttac gtggcgaat gacgtgatt ttggaaccg gatgaacgg actggtgac atttatttt atactttac ggcacgtgt gcgattgaa ctaatctcg gggaaggaa gtgatgcat attgaagat ggggattat ttcgggatt gtcgatatg gaaacggca actgatcct tcgtctatt gtctcgctt gaagtgtcc gaagtttac aaactggag aggaaagat atcaagtat tgctgtaag tgctttcca gaaatcaag cagaaactg gagaagatg gctcttcac aggatgagg aagtttaac caaatgatg agtctgcga ggagttgag gacgagaca accgatgac attattgat aatttgagg aaagggaga atcctacat cggggaata catagacta gctttccta taa
- >TC015742 RA
acggctgtc aaaatgcca gtaaaacga atcgcaaat cgtttcgta cactcgtgt gagctttca caaatcgac gaaaagtcc aaaatccac agcccctac gacacctgc ttccagagc ttctgccac ggtctacgc aagtgcttg acaatagcg aaaaacgtc gacagttca acctacttc cgaagtctt gccgccttc aatcgcgag aaaaaccgc cacctttta tcccgaaag taccgacgc gtcatccat cctttcagc aatttcgca agacgagaa gatttagtc gtttcattc ctctggttt acgattttc gttgtgcaa tcgtacgtt ggcagcttc ttcaaccga gaactccta gacgaaaaa catgcccat gtgtatcga catgtgcta gctttcgca aacttattg ctactctgt gacgtggtt ttgcgcttt ttcgtgggc tatttgatc gacgccacc agaacggtt atcctggac cacaaatcg atcgtcaag aactacctc aagacgtac tttattttt gattttggt gccggtttt agtttttta gtgttctat atttttaac ctcgacgat cgcgcccaa ttgattctg tacgaactg cacactttg cgcatagtg agagtgaga acgattctg aaaaacttg gacaatctt ttgtgcgat ttcaacgtc cgggagtcg atccgcatt gtggtcctg atctccttc atcggcttc ctcagcttg cacactctg acctgcgct tttggcttg atcccagtc taccggaag gtgttccag attcaatac gagttcgag aactcctgg ttatctcag accgacaac cactccccg cagacgcag ttcgagtcc ggctgggag cacactcac agttccctc cagaagtac ctgcaccac ctctccatg gttctgtgc cacttcgtc ggttgtgcg atcccaaag tacaagact gcagactac ttggaaatg ctcatcctg gcctggatc actatcttc ggtctgatt ttctacgtc tatgtcgtc gccaaagtg ctccaactc ttcggcgtg gtcaacatc tccgaaacc aagttcgaa gaattgaag atccagtcg gacaagtat atgcgaagg aacgacttt ccgggggat ttacgcaaa cgcgtcaac gactactat gactacaag ttcgacaag aagtttttc tccgaacag aaaatcctt gattcgctc agtgaacat ctccggatg gaagttctt ctttacagt tgccggaat ctaatcgaa gaagtgcag attttcaag ggattgtcc aggtcagcc attggctcg gttctggcg cttttgaag caggaggtt tacgtggcg aatgacgtg attttggaa ccggatgaa cggactggt gacatttat tttatactt tacggcacg tgtgcgatt gaactaatc tcggggaag gaagtgatg catattgaa gatggggat tatttcggg attgtcgat atggaaacg gcaactgat ccttcgtct attgtctcg cttgaagtg tccgaagtt tacaaactg gagaggaaa gatatcaag tattgctgt aagtgcttt ccagaaatc aagcagaaa ctggagaag atggctctt cacaggatg aggaagttt aaccaaatg atgagtctg cgaggagtt gaggacgag acaaccgat gacattatt gataatttg aggaaaggg agaatccta catcgggga atacataga ctagctttc ctataagtt tgtagattt ttaagcaat aaaaatgac caagttcca acttttttt tctacaagg aggcataaa ccaataaaa acttcccta aaac
