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Gene information
General information
TC015721 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5751914..5759768
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015721
    iBeetle screen iB_05885

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015721 RA
      MLKKFLILT ALLSAISPT SAENVDEFL AKVKKNYDN 
      AKRSKRFID PYEYPGAEQ PLDEMSKYD FIVVGSGSS 
      GSVIANRLT ETNWTVLLL EVGEEATPL TDIPVIAPL 
      FQFTSLNWN YLMEKQDNM CLGLEDQRM AWPRGRGLG 
      GSTLINYMI HVRGNRRDY NRWAKMGNP GWSYHDIFQ 
      YFLKSEDFL VRKQDPGYH TTGGYLGVQ DVPYRTQSA 
      HAFVQAAQE AGHKFVDYN GKRQMGVSY VHATTRNGK 
      RSSAEEAFL RPIKHRQNL KISTKSRVT KVLIDPQTR 
      QAYGVQYIK NGKYHTVLA SKEVILSAG AFNSPQILM 
      LSGIGPQKH LQELGIPVL EDLPVGQKM YDHITFLGL 
      VFQVNESIV SDQKLLESP ESFLQLVLK NNGPLTTLG 
      GVEALLYFK TNVSKGPAP YPDMELIFI SGSMNTDLG 
      KYYRKTFRI TDEVYNTVW KPLENKYTF SVLPMLVHP 
      ESYGHLELK STNPFHWPR FYGNYFTDR DNTDIKTFI 
      AAIREVQRI AKMPTWQKY GVRQVTTKI PGCQNFVFD 
      SDDYWECAL RHVTTTLHH QVATCKMGP KTDPEAVVD 
      PELRVYGVR GLRVADTSV IPIPLTAHT NVPAFMVGE 
      KAADLIKET WRGATVLSS PFKLTEQYI EEFKTGIES 
      LKKLAHEHK FAEKNFDFN LHNGSEPVP DDIEYDFII 
      IGAGASGSV IANRLTERP EWKVLLLEA GGPETPYTR 
      IPRLGHLLQ NSDYNWAYT TTPQKNWCK GMIDGSCAI 
      AGGKALGGG TAINGMMFT RGHPKDYDK WADLGNPGW 
      CYNDVLPYF KKLEDADLK EFDHKYHNR GGPFHIEHP 
      QHQTHLTHD VLQAGKELG LETIDYNGK EQMGLGVLQ 
      MNSKHGVRQ STATAYLEP AEKRQNLFV KPLSHVTKI 
      LIAPHTKEA TGVEYLHND KLHIAKATK EIILSAGAL 
      NTPQILMLS GIGPKEQLE KFEIPVVHE LPVGKHLKD 
      HIGFYGLDL LYNGTESTP DPHYDEVID YLKNGKGPL 
      TTTGCEVVG YLQTEASKD QIKYPDIEL FFSSRKVNA 
      KPSTNPFRL KPEILDSLY KPIDGKKIV NIGVMLTHP 
      KSTGTVTLK DKDPLHHPL VDPNQLSDP EDHDLNTLL 
      HGIRKALAF AGTETFKKL HLEVNEHPV AGCEEHKWG 
      TDEYWKCAI KHLSISLRH VSGTAKMGP GNDKEAVVD 
      HELRVHGVQ KLRVADASV IPVSVTGHT MAPAIMVGE 
      KAAD
    CDS sequence
    • >TC015721 RA
      atgttaaag aaatttctc attttaaca gcgctcttg 
      agtgccata tctcccaca tccgccgaa aacgtggac 
      gaattcctc gccaaagtc aagaaaaat tacgacaat 
      gccaaaagg tcaaaacgc tttattgat ccatacgag 
      taccctggc gctgagcaa cccttagac gaaatgtct 
      aaatacgac ttcatcgtg gtcggttct ggctcatcc 
      ggttcggtt atagccaac cgtttaacc gaaaccaac 
      tggaccgtc cttttgctc gaagtcggc gaagaagcg 
      accccactt accgacatt ccggtcata gcaccctta 
      ttccaattc actagtctc aactggaac tatctcatg 
      gaaaaacaa gacaatatg tgtctaggt ttggaagac 
      caacgcatg gcatggccc cgtggtcgt ggcctgggt 
      ggctcaacc cttatcaat tacatgatc cacgtgcgt 
      ggtaaccgc cgcgattac aaccgatgg gcaaaaatg 
      ggcaacccg ggatggtcg taccacgac atcttccag 
      tacttcctc aaatcagag gatttcctg gtcaggaag 
      caagaccca ggttaccac acaactggt gggtacttg 
      ggggtccag gatgtaccg tatcggacc caatccgcc 
      catgcgttt gtccaagcg gcccaagaa gccggtcat 
      aagttcgtc gattacaat ggtaaacgc caaatggga 
      gtatcatac gtccatgcc accaccaga aatggtaaa 
      agatcaagt gcagaagaa gctttcctc agaccaatc 
      aaacaccgc caaaacctc aaaatctca accaaaagc 
      cgcgtcact aaagtacta atcgatcct caaacccgg 
      caagcatat ggtgtccaa tacatcaaa aatggaaag 
      taccacacg gtactagcc tccaaagag gtgatttta 
      tcggctggt gccttcaat tcgccccaa attttgatg 
      ctatcgggg atcggaccc caaaaacac ctccaggag 
      ttgggaatc cctgtcctg gaggaccta ccagttggg 
      caaaaaatg tacgaccat atcaccttc ctgggcttg 
      gttttccaa gtcaatgag tcgattgtc agtgaccag 
      aaactactc gaaagccca gaatcgttt ctacagcta 
      gtactcaaa aataatgga cctttgaca acccttggt 
      ggggttgaa gccttactg tacttcaaa accaacgtt 
      tccaaaggc cctgcccct taccccgac atggaacta 
      atcttcatt tcgggaagt atgaacaca gatcttgga 
      aaatactac cggaagact ttccgtatt accgacgaa 
      gtttacaat acagtgtgg aaaccccta gagaacaaa 
      tacacattt tcggtacta cctatgctc gtgcacccc 
      gaatcttac ggccacttg gagctaaaa tcaaccaat 
      ccgttccat tggccccgt ttttacggt aattacttt 
      acagatcgc gacaatacc gatattaaa accttcatt 
      gctgcgatc agggaggta caaagaatt gccaaaatg 
      ccaacatgg caaaaatac ggtgtcaga caagttact 
      acaaaaatc cccggttgc caaaatttc gtttttgac 
      agtgatgac tactgggaa tgtgctcta agacacgtt 
      actacgact ttgcaccac caggtggcc acgtgtaag 
      atgggtccg aagactgat cctgaggca gttgttgat 
      ccggaattg agggtgtac ggggtgagg gggttgagg 
      gttgctgat acgagcgtg atcccgata cctttgact 
      gcgcatacg aatgttccc gcgtttatg gtgggggaa 
      aaggcggct gatttgatt aaggagact tggaggggg 
      gctaccgtc ctctcctcc cccttcaaa ctaaccgaa 
      caatacatt gaagaattc aaaaccggc atcgaatcc 
      ctgaaaaag ctcgcccac gagcacaaa ttcgccgaa 
      aaaaacttt gatttcaat ctccacaat ggctcagaa 
      ccagtccca gacgacatc gagtacgac tttatcata 
      atcggcgct ggtgcctcc ggttcggtt atcgcaaac 
      cgattaacc gaacgtccc gaatggaaa gtcctcctc 
      ttggaggct ggaggaccc gaaaccccc tacacccgc 
      atcccccgc ctaggccac ctcctccaa aacagcgac 
      tacaactgg gcctacacc accacccct cagaaaaac 
      tggtgcaag ggtatgatc gatggtagc tgcgccata 
      gctgggggc aaggccctt gggggtggt accgccatc 
      aatgggatg atgtttaca agggggcac cccaaggac 
      tacgacaaa tgggccgat ttgggcaac cctgggtgg 
      tgctacaac gacgtacta ccatatttc aagaaactg 
      gaagacgcg gatttgaag gagtttgac cacaagtac 
      cataatcgt ggaggaccg ttccatatc gagcaccca 
      caacaccag acacacttg acccatgat gtgctacaa 
      gcgggcaaa gagctagga cttgagacc attgattac 
      aatgggaag gaacagatg ggacttggg gtactacaa 
      atgaactcc aaacatggc gttcggcaa agtaccgcc 
      acagcctac ttggaacca gccgagaag agacaaaac 
      ttatttgtc aagcctttg agtcatgtg accaaaatt 
      ttgatagcc ccccataca aaggaagca accggggtt 
      gagtacctc cacaatgac aaactccac attgccaaa 
      gccaccaaa gaaattatt ctatcagct ggtgccctc 
      aacaccccc caaatcctc atgttatca ggaataggc 
      ccaaaagaa caattagaa aagtttgaa atcccagta 
      gttcacgaa ctaccagtc ggcaagcac ctcaaagac 
      cacataggt ttctatggc ctggacttg ctctacaat 
      ggtaccgaa tcaactcca gacccccac tatgacgaa 
      gtcatcgac tatttgaaa aacggcaaa ggcccattg 
      accacaacc ggctgcgaa gtcgtaggc tacctccaa 
      accgaagct tccaaagac caaatcaag taccccgac 
      atcgaacta ttcttctcc tcccgcaaa gtcaacgcc 
      aaaccctcc acaaacccc ttccgcctc aaacccgaa 
      atcctcgac tccctctac aaacccatc gatggtaaa 
      aaaatcgtc aacatcggc gtcatgcta acccacccc 
      aaatccacc ggtactgtc accctcaaa gacaaagac 
      cccctccac cacccccta gtcgacccc aaccagctc 
      tccgacccc gaagaccac gacctcaac accctcctg 
      cacggcatt cgcaaagct ctggctttt gcaggtact 
      gaaacgttc aaaaagttg catttggag gtgaatgaa 
      catccagtg gcgggctgt gaggagcat aaatggggg 
      acggacgag tactggaag tgcgcaatc aagcatttg 
      agtatcagt ttgaggcat gtgagtggt accgccaag 
      atggggcca ggcaatgat aaggaggcg gtggttgac 
      cacgaattg agggtgcac ggggtgcag aaattgagg 
      gttgcggat gcgagtgtc atccctgtg agtgtgact 
      ggacatacc atggccccg gccattatg gtgggggag 
      aaggcggcg gattaa
    mRNA sequence
    • >TC015721 RA
      gttcgtaaa aattgtcgc gttgcaaaa attgccaga 
      acgttttga aaaatgtta aagaaattt ctcatttta 
      acagcgctc ttgagtgcc atatctccc acatccgcc 
      gaaaacgtg gacgaattc ctcgccaaa gtcaagaaa 
      aattacgac aatgccaaa aggtcaaaa cgctttatt 
      gatccatac gagtaccct ggcgctgag caaccctta 
      gacgaaatg tctaaatac gacttcatc gtggtcggt 
      tctggctca tccggttcg gttatagcc aaccgttta 
      accgaaacc aactggacc gtccttttg ctcgaagtc 
      ggcgaagaa gcgacccca cttaccgac attccggtc 
      atagcaccc ttattccaa ttcactagt ctcaactgg 
      aactatctc atggaaaaa caagacaat atgtgtcta 
      ggtttggaa gaccaacgc atggcatgg ccccgtggt 
      cgtggcctg ggtggctca acccttatc aattacatg 
      atccacgtg cgtggtaac cgccgcgat tacaaccga 
      tgggcaaaa atgggcaac ccgggatgg tcgtaccac 
      gacatcttc cagtacttc ctcaaatca gaggatttc 
      ctggtcagg aagcaagac ccaggttac cacacaact 
      ggtgggtac ttgggggtc caggatgta ccgtatcgg 
      acccaatcc gcccatgcg tttgtccaa gcggcccaa 
      gaagccggt cataagttc gtcgattac aatggtaaa 
      cgccaaatg ggagtatca tacgtccat gccaccacc 
      agaaatggt aaaagatca agtgcagaa gaagctttc 
      ctcagacca atcaaacac cgccaaaac ctcaaaatc 
      tcaaccaaa agccgcgtc actaaagta ctaatcgat 
      cctcaaacc cggcaagca tatggtgtc caatacatc 
      aaaaatgga aagtaccac acggtacta gcctccaaa 
      gaggtgatt ttatcggct ggtgccttc aattcgccc 
      caaattttg atgctatcg gggatcgga ccccaaaaa 
      cacctccag gagttggga atccctgtc ctggaggac 
      ctaccagtt gggcaaaaa atgtacgac catatcacc 
      ttcctgggc ttggttttc caagtcaat gagtcgatt 
      gtcagtgac cagaaacta ctcgaaagc ccagaatcg 
      tttctacag ctagtactc aaaaataat ggacctttg 
      acaaccctt ggtggggtt gaagcctta ctgtacttc 
      aaaaccaac gtttccaaa ggccctgcc ccttacccc 
      gacatggaa ctaatcttc atttcggga agtatgaac 
      acagatctt ggaaaatac taccggaag actttccgt 
      attaccgac gaagtttac aatacagtg tggaaaccc 
      ctagagaac aaatacaca ttttcggta ctacctatg 
      ctcgtgcac cccgaatct tacggccac ttggagcta 
      aaatcaacc aatccgttc cattggccc cgtttttac 
      ggtaattac tttacagat cgcgacaat accgatatt 
      aaaaccttc attgctgcg atcagggag gtacaaaga 
      attgccaaa atgccaaca tggcaaaaa tacggtgtc 
      agacaagtt actacaaaa atccccggt tgccaaaat 
      ttcgttttt gacagtgat gactactgg gaatgtgct 
      ctaagacac gttactacg actttgcac caccaggtg 
      gccacgtgt aagatgggt ccgaagact gatcctgag 
      gcagttgtt gatccggaa ttgagggtg tacggggtg 
      agggggttg agggttgct gatacgagc gtgatcccg 
      atacctttg actgcgcat acgaatgtt cccgcgttt 
      atggtgggg gaaaaggcg gctgatttg attaaggag 
      acttggagg ggggctacc gtcctctcc tcccccttc 
      aaactaacc gaacaatac attgaagaa ttcaaaacc 
      ggcatcgaa tccctgaaa aagctcgcc cacgagcac 
      aaattcgcc gaaaaaaac tttgatttc aatctccac 
      aatggctca gaaccagtc ccagacgac atcgagtac 
      gactttatc ataatcggc gctggtgcc tccggttcg 
      gttatcgca aaccgatta accgaacgt cccgaatgg 
      aaagtcctc ctcttggag gctggagga cccgaaacc 
      ccctacacc cgcatcccc cgcctaggc cacctcctc 
      caaaacagc gactacaac tgggcctac accaccacc 
      cctcagaaa aactggtgc aagggtatg atcgatggt 
      agctgcgcc atagctggg ggcaaggcc cttgggggt 
      ggtaccgcc atcaatggg atgatgttt acaaggggg 
      caccccaag gactacgac aaatgggcc gatttgggc 
      aaccctggg tggtgctac aacgacgta ctaccatat 
      ttcaagaaa ctggaagac gcggatttg aaggagttt 
      gaccacaag taccataat cgtggagga ccgttccat 
      atcgagcac ccacaacac cagacacac ttgacccat 
      gatgtgcta caagcgggc aaagagcta ggacttgag 
      accattgat tacaatggg aaggaacag atgggactt 
      ggggtacta caaatgaac tccaaacat ggcgttcgg 
      caaagtacc gccacagcc tacttggaa ccagccgag 
      aagagacaa aacttattt gtcaagcct ttgagtcat 
      gtgaccaaa attttgata gccccccat acaaaggaa 
      gcaaccggg gttgagtac ctccacaat gacaaactc 
      cacattgcc aaagccacc aaagaaatt attctatca 
      gctggtgcc ctcaacacc ccccaaatc ctcatgtta 
      tcaggaata ggcccaaaa gaacaatta gaaaagttt 
      gaaatccca gtagttcac gaactacca gtcggcaag 
      cacctcaaa gaccacata ggtttctat ggcctggac 
      ttgctctac aatggtacc gaatcaact ccagacccc 
      cactatgac gaagtcatc gactatttg aaaaacggc 
      aaaggccca ttgaccaca accggctgc gaagtcgta 
      ggctacctc caaaccgaa gcttccaaa gaccaaatc 
      aagtacccc gacatcgaa ctattcttc tcctcccgc 
      aaagtcaac gccaaaccc tccacaaac cccttccgc 
      ctcaaaccc gaaatcctc gactccctc tacaaaccc 
      atcgatggt aaaaaaatc gtcaacatc ggcgtcatg 
      ctaacccac cccaaatcc accggtact gtcaccctc 
      aaagacaaa gaccccctc caccacccc ctagtcgac 
      cccaaccag ctctccgac cccgaagac cacgacctc 
      aacaccctc ctgcacggc attcgcaaa gctctggct 
      tttgcaggt actgaaacg ttcaaaaag ttgcatttg 
      gaggtgaat gaacatcca gtggcgggc tgtgaggag 
      cataaatgg gggacggac gagtactgg aagtgcgca 
      atcaagcat ttgagtatc agtttgagg catgtgagt 
      ggtaccgcc aagatgggg ccaggcaat gataaggag 
      gcggtggtt gaccacgaa ttgagggtg cacggggtg 
      cagaaattg agggttgcg gatgcgagt gtcatccct 
      gtgagtgtg actggacat accatggcc ccggccatt 
      atggtgggg gagaaggcg gcggattaa tcaagaagt 
      tggaaataa tttttgtgt attataaat tcgtaataa 
      aaactagat tagtta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY