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Gene information
General information
TC015719 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5746584..5749199
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015719
    iBeetle screen iB_05884

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015719 RA
      MLKTVILLT IIANTYQQS VLEGIINIL EEGEAQFNL 
      EPEDVRNLL PEYDFIVVG AGSAGCVVA NRLSENPNW 
      KVLLIEAGR TENYLMDMP ILANYLQFT DSNWKYKTT 
      PSGRFCMGM DNQQCKWPR GKVVGGSSV LKYMIYTRE 
      NHRDYDHWA DLGNTGWSF KEVLPYFKK VENFSVPDS 
      PYPEYHSKE GYLSVSYAP FKTKIADAI IEASNQNGI 
      KSVDYNGPI QVGVSRLQV SMRDGVRES ASRAYLHPI 
      RNRPNLHVK KLAMVSKVL IDPKTKQTI GVEFFRDGT 
      RYQIRASKE VIVSAGAIN SPQLLMLSG IGPRKHLTQ 
      KGIPVLSNL KVGYNLMDH IALGGLTFI INKPYSLNT 
      EKMITTENM RQYLNYHKG PLSVPGGCE VLVFHDLKN 
      PTDPDGYPD IELLFQGGS IVSDPLLRK DFGITNELY 
      DAVYKPIED LDTFMVFPM LMRPKSKGR IMLKNNNYR 
      AKPYIYPNY FAYDEDMDT IMGGVHLIL NITQQPALQ 
      ALGARLHDI PIPQCAKYG FASDDYFKC MARHFTFTI 
      YHQSGTCKM GPPSDKKAV VDPRLRVYG IKGLRVIDA 
      SIMPEVPAA HTNSPTFMI AEKGADLIK EDWANRS
    CDS sequence
    • >TC015719 RA
      atgctaaaa acagtgatt ctcctgacc atcattgcc 
      aacacctac caacaaagt gtcttggag ggaatcatc 
      aacatcctc gaggagggc gaagcccaa tttaacctc 
      gagcctgaa gatgtgcga aacctcctc ccagagtac 
      gattttatc gtagtaggc gccgggagc gccggctgc 
      gtcgttgcc aaccgtctc tccgaaaac cccaactgg 
      aaagtgcta ctaatcgaa gcaggtcgt acggagaac 
      tacctaatg gatatgccc atcctagcc aactacctg 
      caattcacc gattcgaac tggaagtac aaaaccacc 
      cccagtggg cgtttctgc atggggatg gacaaccag 
      cagtgcaaa tggccgaga gggaaagtc gtgggcggg 
      tcaagtgtc ctcaaatac atgatctac acccgggag 
      aaccaccgg gattacgac cactgggcc gatttgggc 
      aacacaggg tggtccttc aaagaggtc ctcccttat 
      ttcaaaaaa gtggaaaat ttctccgtc cccgactcc 
      ccctacccc gagtaccac tcaaaagag gggtacctc 
      tcggtcagt tatgcccct tttaaaacc aagatcgcc 
      gatgccatt atagaagcc agtaaccaa aacggtatt 
      aaatccgtc gattacaac ggccccatc caagtaggc 
      gtgtcccga ctccaagtc tcgatgcga gatggggtc 
      cgggagagt gccagtagg gcctacctc caccccatt 
      cgaaaccgc cccaacctc cacgtcaaa aaactcgcc 
      atggtcagc aaggtacta atcgacccc aagaccaaa 
      caaacaata ggcgttgaa ttcttccgg gatggtacc 
      aggtaccag atccgggcc tcgaaagag gtcatcgtc 
      tcagcaggt gccataaac tcgccacaa ctcctcatg 
      ttgagcgga atcgggccc cggaaacac ctcacccag 
      aaaggtatt ccggtactg tccaacctt aaggttggc 
      tacaacctc atggaccac atcgccttg ggcggtctc 
      actttcatc atcaacaaa ccgtactcg ctcaacacc 
      gaaaaaatg ataaccacg gaaaacatg cgacagtac 
      ttgaactac cacaaaggc ccgttatcg gtacccgga 
      ggctgcgag gtcctggtc ttccatgac ctgaagaac 
      cccacggac ccagacggg taccccgac attgaactg 
      ctgttccaa ggcggttcc atcgtttcc gacccctta 
      ctgcgcaaa gattttggc ataacaaac gaactctat 
      gacgcggtt tacaaaccc attgaggac ctagacacc 
      ttcatggtt ttccccatg ttgatgaga cccaaaagt 
      aaaggtcgc atcatgctc aagaacaac aactaccgg 
      gcaaaaccc tacatctac cccaattac ttcgcctat 
      gacgaggac atggacacc atcatgggt ggcgtccat 
      ctaatccta aacatcacc caacagccc gccctccag 
      gcactcggt gcccgcctc catgacatc cccatcccc 
      caatgtgcc aaatacggc ttcgcctcc gacgattac 
      ttcaaatgt atggcacgc catttcacc ttcaccatc 
      taccatcag agtggtact tgcaaaatg gggccaccc 
      agcgataaa aaagcagtg gtggaccca cgattaagg 
      gtttatggt attaaaggg ttgcgggtt attgatgcc 
      tccatcatg cctgaagta ccggcggct catacgaat 
      tcgcccact tttatgatt gcggaaaaa ggggccgat 
      ttgatcaaa gaagactgg gcgaataga agctga
    mRNA sequence
    • >TC015719 RA
      tggtggtga aatgatgag ttttgcgtg acaagggcc 
      tgagaagtt catttaatt ttgttcatt tgacgtcaa 
      attaagcaa aaatgctaa aaacagtga ttctcctga 
      ccatcattg ccaacacct accaacaaa gtgtcttgg 
      agggaatca tcaacatcc tcgaggagg gcgaagccc 
      aatttaacc tcgagcctg aagatgtgc gaaacctcc 
      tcccagagt acgatttta tcgtagtag gcgccggga 
      gcgccggct gcgtcgttg ccaaccgtc tctccgaaa 
      accccaact ggaaagtgc tactaatcg aagcaggtc 
      gtacggaga actacctaa tggatatgc ccatcctag 
      ccaactacc tgcaattca ccgattcga actggaagt 
      acaaaacca cccccagtg ggcgtttct gcatgggga 
      tggacaacc agcagtgca aatggccga gagggaaag 
      tcgtgggcg ggtcaagtg tcctcaaat acatgatct 
      acacccggg agaaccacc gggattacg accactggg 
      ccgatttgg gcaacacag ggtggtcct tcaaagagg 
      tcctccctt atttcaaaa aagtggaaa atttctccg 
      tccccgact ccccctacc ccgagtacc actcaaaag 
      aggggtacc tctcggtca gttatgccc cttttaaaa 
      ccaagatcg ccgatgcca ttatagaag ccagtaacc 
      aaaacggta ttaaatccg tcgattaca acggcccca 
      tccaagtag gcgtgtccc gactccaag tctcgatgc 
      gagatgggg tccgggaga gtgccagta gggcctacc 
      tccacccca ttcgaaacc gccccaacc tccacgtca 
      aaaaactcg ccatggtca gcaaggtac taatcgacc 
      ccaagacca aacaaacaa taggcgttg aattcttcc 
      gggatggta ccaggtacc agatccggg cctcgaaag 
      aggtcatcg tctcagcag gtgccataa actcgccac 
      aactcctca tgttgagcg gaatcgggc cccggaaac 
      acctcaccc agaaaggta ttccggtac tgtccaacc 
      ttaaggttg gctacaacc tcatggacc acatcgcct 
      tgggcggtc tcactttca tcatcaaca aaccgtact 
      cgctcaaca ccgaaaaaa tgataacca cggaaaaca 
      tgcgacagt acttgaact accacaaag gcccgttat 
      cggtacccg gaggctgcg aggtcctgg tcttccatg 
      acctgaaga accccacgg acccagacg ggtaccccg 
      acattgaac tgctgttcc aaggcggtt ccatcgttt 
      ccgacccct tactgcgca aagattttg gcataacaa 
      acgaactct atgacgcgg tttacaaac ccattgagg 
      acctagaca ccttcatgg ttttcccca tgttgatga 
      gacccaaaa gtaaaggtc gcatcatgc tcaagaaca 
      acaactacc gggcaaaac cctacatct accccaatt 
      acttcgcct atgacgagg acatggaca ccatcatgg 
      gtggcgtcc atctaatcc taaacatca cccaacagc 
      ccgccctcc aggcactcg gtgcccgcc tccatgaca 
      tccccatcc cccaatgtg ccaaatacg gcttcgcct 
      ccgacgatt acttcaaat gtatggcac gccatttca 
      ccttcacca tctaccatc agagtggta cttgcaaaa 
      tggggccac ccagcgata aaaaagcag tggtggacc 
      cacgattaa gggtttatg gtattaaag ggttgcggg 
      ttattgatg cctccatca tgcctgaag taccggcgg 
      ctcatacga attcgccca cttttatga ttgcggaaa 
      aaggggccg atttgatca aagaagact gggcgaata 
      gaagctgac atttactgt gattcgtta ttttaattg 
      taattatat taattaaat aatttaaat aatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY