Status
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Gene information
General information
TC015718 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5737092..5740400
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015718
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05883

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015718 RA
      MVVETLFIS TALKTIGIV GSTLWIIPL IFAGISYYH 
      YDKLDPESP IINRRTLYK EYDFVVVGG GSAGAVVAS 
      RLSEIPSWN VLLLEAGPD ENEISDVPS LAAYLQLSK 
      LDWTYKTEP TGRACLGMN NGRCNWPRG KVLGGSSVL 
      NYMLYVRGN RHDYDQWEA MGNHGWNYE NVLHYFKKS 
      EDNRNPYLA RTKYHNQGG LLTVQESPW RTPLVLAFV 
      QAGTELGYP NRDINGAEQ AGFMVAQGT IRRGSRCST 
      AKAFLRPIR LRKNIHIAL NSHVTRVLI NPSTMRAFG 
      VEFVRNGHK QIVLARKEV IMSAGAINT PQILMLSGI 
      GPQPQLSKF GIPVLRDLP VGENLQDHV GMGGFTFLV 
      DKPVSIVQD RFQAFPMTM QYVMNAKGP MTTLGGVEG 
      LAFVNTKYG NRSWPDVQF HMAPASINS DAGVRVRKV 
      LGLTDHLYN TVYRPIANK DVFTLMPLL LRPKSRGWI 
      RLQSKNPFV PPVINANYF DDPIDIKVL VEGAKMAIK 
      IGEAQAFKQ FGARVHRIK FPNCRDFEF GSDEYLECH 
      IRTISMTIY HPVGTCKMG PSWDKEAVV DPRLKVYGV 
      EGLRVIDAS IMPTIPSGN TNAPAIMVG EKGADLVKE 
      DWLQR
    CDS sequence
    • >TC015718 RA
      atggtagtc gaaaccctg ttcatatca accgccctt 
      aaaaccatc ggaatcgtc ggttcaacc ctctggatc 
      atccctctg atcttcgct ggaatttcc tactaccac 
      tacgacaaa ctcgacccg gaatcgccc ataatcaac 
      cgaagaacg ctctacaaa gagtacgac ttcgtagtt 
      gtagggggc ggttcagcg ggggcggtt gtagcctcg 
      cgcctatcc gaaatcccc tcttggaat gtgctcctc 
      ctggaggcc ggaccggac gaaaatgag atctcggac 
      gtcccctca ttggccgct tatctccag ctgtcgaaa 
      ctcgattgg acgtataag acggaaccg acggggagg 
      gcgtgtctt gggatgaac aatgggcgg tgcaattgg 
      cccagaggc aaggttttg ggcgggtcc agtgtactg 
      aactatatg ctgtacgtg cgggggaat cggcacgat 
      tacgaccaa tgggaggcc atggggaac catggttgg 
      aactacgaa aatgttctc cactacttc aaaaaatcg 
      gaagataac aggaacccg tacttggcg cgtaccaaa 
      tatcacaac caagggggt ttattgaca gttcaggag 
      tccccatgg aggaccccc ttagtcctg gccttcgtc 
      caggcgggt actgagcta gggtacccc aaccgggac 
      ataaatggt gccgaacag gccggtttc atggtcgcc 
      cagggtacc ataagacgt ggttcccgt tgttccaca 
      gccaaagcc ttcctccgc cccatcaga ctccgtaaa 
      aacatccac atcgcctta aactcccac gtgactcga 
      gtcctaatc aacccatca accatgcgg gctttcggg 
      gtcgaattc gtccgaaac ggccataaa cagatcgtt 
      ttggcgcga aaagaagtc ataatgagc gccggcgcc 
      atcaacact cctcaaatc ctcatgcta tcaggcatc 
      ggtccccag ccccagctc tccaagttc gggatccca 
      gtcctgagg gacctcccc gtcggggag aacctccag 
      gaccatgtg ggcatgggc ggtttcacg tttctagtc 
      gacaaaccc gtctccata gtccaggac cgcttccag 
      gccttcccc atgaccatg cagtacgtc atgaacgcc 
      aaaggcccc atgaccacc ctcggaggg gtcgaaggc 
      ctggccttc gtcaacacg aaatacggt aaccgttcc 
      tggcccgac gtccaattc cacatggca cctgccagt 
      ataaattcg gacgccggc gtccgagtc cgtaaagtt 
      ttaggactt acggaccac ctctacaac acggtgtac 
      cgccctatc gccaataag gacgtcttc acgcttatg 
      cccctcctc ctgaggccc aaatcgcgc ggttggatc 
      cgcctccaa agcaaaaat cccttcgtg ccgcctgtg 
      atcaacgcc aactatttt gacgatccc attgatatt 
      aaagtacta gtggagggg gcaaagatg gcgattaaa 
      atcggcgaa gcccaagct tttaagcag tttggggca 
      agagtgcat cgaatcaag tttccgaat tgtcgggat 
      tttgagttt gggagtgat gagtatttg gagtgccat 
      ataaggacg atttcgatg acgatttac catccggtg 
      gggacgtgc aagatgggg ccgagttgg gataaggag 
      gctgtggtt gatcccagg ttgaaggtc tatggagtg 
      gaggggttg agggtgatt gacgcctct atcatgccg 
      accattccg agtgggaat acgaacgca ccggcgatt 
      atggtgggg gaaaaaggg gcggatctg gtcaaagag 
      gattggcta caacgatag
    mRNA sequence
    • >TC015718 RA
      ggactaaaa tgaaaatcc cgaaaaatg gtagtcgaa 
      accctgttc atatcaacc gcccttaaa accatcgga 
      atcgtcggt tcaaccctc tggatcatc cctctgatc 
      ttcgctgga atttcctac taccactac gacaaactc 
      gacccggaa tcgcccata atcaaccga agaacgctc 
      tacaaagag tacgacttc gtagttgta gggggcggt 
      tcagcgggg gcggttgta gcctcgcgc ctatccgaa 
      atcccctct tggaatgtg ctcctcctg gaggccgga 
      ccggacgaa aatgagatc tcggacgtc ccctcattg 
      gccgcttat ctccagctg tcgaaactc gattggacg 
      tataagacg gaaccgacg gggagggcg tgtcttggg 
      atgaacaat gggcggtgc aattggccc agaggcaag 
      gttttgggc gggtccagt gtactgaac tatatgctg 
      tacgtgcgg gggaatcgg cacgattac gaccaatgg 
      gaggccatg gggaaccat ggttggaac tacgaaaat 
      gttctccac tacttcaaa aaatcggaa gataacagg 
      aacccgtac ttggcgcgt accaaatat cacaaccaa 
      gggggttta ttgacagtt caggagtcc ccatggagg 
      accccctta gtcctggcc ttcgtccag gcgggtact 
      gagctaggg taccccaac cgggacata aatggtgcc 
      gaacaggcc ggtttcatg gtcgcccag ggtaccata 
      agacgtggt tcccgttgt tccacagcc aaagccttc 
      ctccgcccc atcagactc cgtaaaaac atccacatc 
      gccttaaac tcccacgtg actcgagtc ctaatcaac 
      ccatcaacc atgcgggct ttcggggtc gaattcgtc 
      cgaaacggc cataaacag atcgttttg gcgcgaaaa 
      gaagtcata atgagcgcc ggcgccatc aacactcct 
      caaatcctc atgctatca ggcatcggt ccccagccc 
      cagctctcc aagttcggg atcccagtc ctgagggac 
      ctccccgtc ggggagaac ctccaggac catgtgggc 
      atgggcggt ttcacgttt ctagtcgac aaacccgtc 
      tccatagtc caggaccgc ttccaggcc ttccccatg 
      accatgcag tacgtcatg aacgccaaa ggccccatg 
      accaccctc ggaggggtc gaaggcctg gccttcgtc 
      aacacgaaa tacggtaac cgttcctgg cccgacgtc 
      caattccac atggcacct gccagtata aattcggac 
      gccggcgtc cgagtccgt aaagtttta ggacttacg 
      gaccacctc tacaacacg gtgtaccgc cctatcgcc 
      aataaggac gtcttcacg cttatgccc ctcctcctg 
      aggcccaaa tcgcgcggt tggatccgc ctccaaagc 
      aaaaatccc ttcgtgccg cctgtgatc aacgccaac 
      tattttgac gatcccatt gatattaaa gtactagtg 
      gagggggca aagatggcg attaaaatc ggcgaagcc 
      caagctttt aagcagttt ggggcaaga gtgcatcga 
      atcaagttt ccgaattgt cgggatttt gagtttggg 
      agtgatgag tatttggag tgccatata aggacgatt 
      tcgatgacg atttaccat ccggtgggg acgtgcaag 
      atggggccg agttgggat aaggaggct gtggttgat 
      cccaggttg aaggtctat ggagtggag gggttgagg 
      gtgattgac gcctctatc atgccgacc attccgagt 
      gggaatacg aacgcaccg gcgattatg gtgggggaa 
      aaaggggcg gatctggtc aaagaggat tggctacaa 
      cgatagact ttcgaagtg ataatctaa tttaattct 
      aatacaatt atttatgac gt
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