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Gene information
General information
TC015717 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5728752..5731857
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015717
    iBeetle screen iB_05882

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015717 RA
      MSMLATIGQ VAHFAGSLT GNPLAIIGL VPIFAAGLA 
      FMRYVSIDP EAHPVNVRH VRPEYDFIV VGGGSAGAV 
      VASRLSEIA NWTVLLLEA GGDENEISD IPALSGYTQ 
      MSQFDWMYQ TSPPGDSPY CLAMIGDRC NWPRGKVLG 
      GSSVLNAMI YIRGNRHDY DQWAAMGNT GWSYPEVLP 
      YFLKSEDNR NPYLARTKY HNTGGYLTV QESPWRTPL 
      SIAFLQAGR ELGYEVRDL NGEKQTGFM LSQGTIRRG 
      SRCSTSKAF LRPVKSRQN LHIAMYSQV TKVMIDPKT 
      KTAYGVKFT RNNRPQTVR ARREVILSA GAIGTPHIL 
      MLSGVGEKS HLESFKIPV MSDLKVGYN LQDHIGLGG 
      LTFVIDDPI TFTKTRYQT FAVAMEYIV NERGPMTSL 
      GGVEGLAFV NTKYAPKSG SWPDIQFHF APSSINSDG 
      EQVKKITGL RDSVYNTVY KPLKNAETW TILPLLLRP 
      RSTGWVRLK SKDPNIYPD INPNYFTHK EDILTLTEG 
      IRIALNVSN TQSFQRFNS RPHKIPFPK CRQYDWDSD 
      EYWECSLRH FTFTIYHPT STAKMGPAS DPDAVVDPR 
      LRVYGIKGL RVIDASIMP TIVSGNTNA PTIMIGEKG 
      SDMIKQDWG IRV
    CDS sequence
    • >TC015717 RA
      atgtctatg cttgcgacc ataggacaa gtggcccat 
      tttgccgga agtttgacc ggaaatccg ttggccata 
      atcggatta gtgcccatt tttgccgcc ggacttgct 
      ttcatgcga tatgtctcc atagatccg gaggcgcat 
      ccggtcaat gtccggcat gtcagacct gagtatgac 
      tttatcgtg gttgggggc ggctctgcg ggggcggtt 
      gtggcctcc cgactgtcc gaaatcgcc aactggacc 
      gtcctcctc ttggaggcg ggaggagac gagaacgaa 
      atttccgat attccggct ttgagtggc tacacacaa 
      atgagccaa tttgattgg atgtatcaa acgtcccct 
      cccggggat tccccgtat tgcttggcc atgattgga 
      gaccggtgt aactggcca agggggaag gttcttgga 
      ggatctagt gttctgaac gccatgatc tacatcaga 
      ggaaaccgc catgattat gaccaatgg gcggctatg 
      ggcaataca ggatggtcg taccccgaa gtcctcccc 
      tacttcctc aaatccgag gataacagg aacccatat 
      ctggcccgc accaagtac cacaacacc ggagggtac 
      ctcacggtg caagaatcc ccctggagg accccttta 
      tccattgcg ttcctccaa gccggccgg gagttgggg 
      tacgaagtc cgggacctc aatggcgaa aaacaaacc 
      ggatttatg ctctcccaa ggtaccatc cgacgtggt 
      tcccgctgt agtacctcc aaagccttc ctacgccca 
      gtcaaatcc cgtcaaaac ctccacatc gccatgtat 
      tcccaagta accaaagtg atgatcgac cccaaaacc 
      aaaaccgca tatggggtc aaattcacg cgaaataac 
      cgaccccaa accgtgcgg gcgcgccgc gaggtcata 
      ctttcagcg ggggctatc ggtacccca cacatcctc 
      atgttgtca ggagttggg gaaaaatcc cacttggaa 
      tcgttcaaa atcccggta atgtccgac ctcaaagtc 
      ggttataac ctccaggat cacatcggg ttagggggt 
      ctcacgttc gtgatcgac gaccccatc acattcacc 
      aaaactcgc taccagact tttgccgtg gcaatggaa 
      tacatagtg aatgaaagg ggtccgatg acctcactg 
      ggaggtgtg gaggggcta gcgttcgtc aacacgaaa 
      tatgcccca aaatcgggg tcctggccc gacatacaa 
      ttccacttc gccccctcc agtataaat tccgatggg 
      gaacaagtg aaaaaaatc acaggattg cgagacagt 
      gtgtacaac actgtgtac aaacccctt aaaaacgcc 
      gagacttgg accattcta cccctttta ctcagaccc 
      aggagcacg ggttgggtg cgtctcaag tccaaagac 
      cccaatatc taccccgac attaacccc aattacttc 
      acgcataaa gaggacatt ttgactctc actgaaggg 
      atcaggatt gccctaaac gtctcaaac acccaatcc 
      ttccaacgg ttcaattcc cgaccccat aaaatcccg 
      ttcccgaag tgtcgccaa tacgactgg gattcagac 
      gaatattgg gaatgttcc ctgcgacat ttcaccttc 
      acaatatac catcccact tcgacagcc aaaatgggg 
      ccggccagc gaccccgat gctgtggtc gacccccga 
      ttaagggtt tatgggatc aagggatta cgagtgatc 
      gatgcgtca atcatgccc acgattgtc agcgggaat 
      acaaacgcc ccgacgatc atgatcggc gaaaagggg 
      tcggacatg atcaagcag gattggggt atcagagtt 
      tga
    mRNA sequence
    • >TC015717 RA
      cttgtattg tttcgtgtg cagtgggtg cagtgacta 
      cgtttttat taattaagt tagataatt tggggcaag 
      atggcgacg attttgata tttcttgaa aatcaagtt 
      gcgccgtcc gccagctaa ggaaatggg aaaacctga 
      cccatttcc aaagaaaca caaaagtgc caaaaccaa 
      agaaaagta ccacaccga gtaccacaa tcgcgagaa 
      gaaaactcg cgaaaactt ctacaacac gacccccaa 
      tgagaaaca cgcccagaa cgactattt gaaaaacaa 
      cctccatat caagacttg cgtttgcgg aagtcgcga 
      aatactcaa attccaatc gaagaaaac caacttccg 
      gaattacac gccgaaaat tttgaacca ggaggtcaa 
      catgggcat gtggatgtg acaagatgt ctatgcttg 
      cgaccatag gacaagtgg cccattttg ccggaagtt 
      tgaccggaa atccgttgg ccataatcg gattagtgc 
      ccatttttg ccgccggac ttgctttca tgcgatatg 
      tctccatag atccggagg cgcatccgg tcaatgtcc 
      ggcatgtca gacctgagt atgacttta tcgtggttg 
      ggggcggct ctgcggggg cggttgtgg cctcccgac 
      tgtccgaaa tcgccaact ggaccgtcc tcctcttgg 
      aggcgggag gagacgaga acgaaattt ccgatattc 
      cggctttga gtggctaca cacaaatga gccaatttg 
      attggatgt atcaaacgt cccctcccg gggattccc 
      cgtattgct tggccatga ttggagacc ggtgtaact 
      ggccaaggg ggaaggttc ttggaggat ctagtgttc 
      tgaacgcca tgatctaca tcagaggaa accgccatg 
      attatgacc aatgggcgg ctatgggca atacaggat 
      ggtcgtacc ccgaagtcc tcccctact tcctcaaat 
      ccgaggata acaggaacc catatctgg cccgcacca 
      agtaccaca acaccggag ggtacctca cggtgcaag 
      aatccccct ggaggaccc ctttatcca ttgcgttcc 
      tccaagccg gccgggagt tggggtacg aagtccggg 
      acctcaatg gcgaaaaac aaaccggat ttatgctct 
      cccaaggta ccatccgac gtggttccc gctgtagta 
      cctccaaag ccttcctac gcccagtca aatcccgtc 
      aaaacctcc acatcgcca tgtattccc aagtaacca 
      aagtgatga tcgacccca aaaccaaaa ccgcatatg 
      gggtcaaat tcacgcgaa ataaccgac cccaaaccg 
      tgcgggcgc gccgcgagg tcatacttt cagcggggg 
      ctatcggta ccccacaca tcctcatgt tgtcaggag 
      ttggggaaa aatcccact tggaatcgt tcaaaatcc 
      cggtaatgt ccgacctca aagtcggtt ataacctcc 
      aggatcaca tcgggttag ggggtctca cgttcgtga 
      tcgacgacc ccatcacat tcaccaaaa ctcgctacc 
      agacttttg ccgtggcaa tggaataca tagtgaatg 
      aaaggggtc cgatgacct cactgggag gtgtggagg 
      ggctagcgt tcgtcaaca cgaaatatg ccccaaaat 
      cggggtcct ggcccgaca tacaattcc acttcgccc 
      cctccagta taaattccg atggggaac aagtgaaaa 
      aaatcacag gattgcgag acagtgtgt acaacactg 
      tgtacaaac cccttaaaa acgccgaga cttggacca 
      ttctacccc ttttactca gacccagga gcacgggtt 
      gggtgcgtc tcaagtcca aagacccca atatctacc 
      ccgacatta accccaatt acttcacgc ataaagagg 
      acattttga ctctcactg aagggatca ggattgccc 
      taaacgtct caaacaccc aatccttcc aacggttca 
      attcccgac cccataaaa tcccgttcc cgaagtgtc 
      gccaatacg actgggatt cagacgaat attgggaat 
      gttccctgc gacatttca ccttcacaa tataccatc 
      ccacttcga cagccaaaa tggggccgg ccagcgacc 
      ccgatgctg tggtcgacc cccgattaa gggtttatg 
      ggatcaagg gattacgag tgatcgatg cgtcaatca 
      tgcccacga ttgtcagcg ggaatacaa acgccccga 
      cgatcatga tcggcgaaa aggggtcgg acatgatca 
      agcaggatt ggggtatca gagtttgat cgtcaaaaa 
      ctactagtc aaaaatttg taaatattt tattttgta 
      ctgtatttt gtaattatt tttttatta aaattttat 
      acactttaa acagttatt tttttattc acagatttg 
      ggaattgct taaaaaacc cattctgta aggttttgc 
      aattcgaaa cctctccac taattggca tcaattgtg 
      ctaaaacct cttgcaatt tcggtaatc tgttaacat 
      tgtgtaata taagataat aaaatttca gctgcaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY