Status
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Gene information
General information
TC015712 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5711732..5713919
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015712
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05880

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    remarks: later than schedule, hours 48
    Pupal morphology
    remarks: later than schedule, hours 48
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence broader - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: dead larva
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna antennal flagellum size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    urogomphi orientation irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015712 RA
      MTLIGIMST ITAPLLGLG ATAASNLAV FIPALAAAI 
      AYFQYDLLD PESRPIDVS TDELLERYD FIVVGAGSA 
      GAVVANRLS EIEQWNVLL LEAGGDEME ISDVPLMAA 
      YLQLSQIDW KYKSEPQGQ ACLAMKNGR CNWPRGKVI 
      GGSSVLNYM LYLRGNKKD YDIWESLGN PGWGSQDAL 
      YYFKKSEDN QNPYLSRTP YHATGGYLT VSEAPYHTP 
      LVAAFVEGG RQLGYANRD INGEHQSGF MMAQGTTRR 
      GSRCSTGKA FLRPVRLRK NLHVAMHAH VTKVMVDPT 
      SKVAFGVEF VRDKKLYRI RATKEVVLS AGAVNSPQL 
      LMLSGIGPK EDLERLKIP LVQDLKVGH NLQDHVGLG 
      GLTFLINRP HSILLNRLY SVSSLMQYA IFGGGPLTI 
      MGGVEGLAF VNTKYVNAS DDFPDIELH FISGSTHSD 
      GGTQLRKAH GLTDAFYER VFGPIADKD AWSVIPMLL 
      RPKSRGFIK LRSKNPLDY PLIYPNYFK DDFDMKTLI 
      EGAKLSVAL SQTPAFKYY KSTLHKFPD CAGFKDYSD 
      EFYECMIRL YTVTIYHPV GTCKMGPYW DQEAVVDPQ 
      LRVYGIKGL RVIDASIMP NLVSGNTNA PAIMIGEKG 
      SDLIKEFWI KTARYGRFV
    CDS sequence
    • >TC015712 RA
      atgacactt atcggaatt atgagcacc attacggcc 
      cctttattg ggcctggga gccacggca gcttccaat 
      ttagccgtt tttatccca gctttagca gcagctatc 
      gcctatttc cagtacgac ctgcttgac cccgagtca 
      agacctatc gatgtttcg actgacgaa ttattggaa 
      cggtacgac tttatagtg gttggggcg ggatcagca 
      ggggcggtt gtggcaaac cgacttagt gagatagaa 
      caatggaat gtgctcctg ctggaagca ggaggcgac 
      gagatggaa atttcggac gtcccgctc atggccgcc 
      tacctccag ctatcgcaa atcgactgg aagtacaaa 
      agcgagccc caaggccaa gcctgtctc gccatgaaa 
      aacggccgt tgtaactgg ccccggggc aaagtcatc 
      gggggctcc tccgtcctc aactacatg ttgtacctc 
      cgcggcaat aaaaaagac tacgacatt tgggaatcc 
      ctgggtaac ccgggctgg ggcagccag gacgccctc 
      tactacttc aaaaaatcg gaagacaac caaaatccc 
      tacttgtcc cgcaccccg taccacgcc actgggggc 
      tacttgacg gtgtccgaa gccccctac cacaccccc 
      ctggtggcg gcgttcgtg gaaggcggc cgccaactg 
      ggctacgcc aaccgggac atcaacggc gagcaccaa 
      tccgggttc atgatggcg caaggtacc acccggagg 
      gggtcgcgc tgctcgacc ggaaaagca ttcctgcgt 
      ccggtacgc ctccggaag aacctccac gtggccatg 
      cacgcccac gtgaccaag gtcatggtc gaccccacc 
      agtaaagtg gccttcggg gtcgagttc gtgagggat 
      aagaagttg taccggatt cgggccacc aaagaggtg 
      gtactttcg gcaggcgcg gtcaattcg ccccaactg 
      ctcatgttg tcagggatc gggcctaag gaggatctg 
      gaacggctc aaaatccca cttgtgcag gatttaaag 
      gttgggcac aacctacag gaccatgtg gggttaggg 
      ggattgacg tttctgatc aatcggcca cactcgatc 
      ctcctcaat cggctgtat tcggttagt tcgctaatg 
      cagtacgcg attttcggg ggcggcccc ttgaccatc 
      atgggtggg gtggagggg ttggcgttc gttaacacg 
      aaatatgtc aacgcttcg gacgatttc cccgatatt 
      gagctacat ttcatctcg ggttcgacg cattctgat 
      ggtggtacc cagcttagg aaagcccat gggctcact 
      gacgcgttc tatgagcgg gtttttggg cccattgcc 
      gataaggac gcctggagt gttatccca atgctgcta 
      aggcccaaa tcacgcggt ttcattaaa ttgcggagc 
      aaaaaccca ctggactac cctttaatc taccccaac 
      tacttcaaa gatgatttc gatatgaaa actttgatt 
      gaaggagcg aaactaagc gtggccttg agccaaacc 
      ccggcgttc aagtactac aaaagtacc ctccacaaa 
      ttcccggac tgtgccggt ttcaaagac tactcggat 
      gagttttac gagtgtatg atccgcctc tacactgtc 
      acaatttac catccggtc ggaacgtgc aaaatgggc 
      ccttattgg gaccaggag gccgtcgtg gacccacaa 
      ttgcgcgtt tacggcatc aagggccta agggtgatc 
      gatgcttca ataatgcca aacctagtg agtggcaac 
      acgaacgcc cctgccata atgatcggg gaaaaaggg 
      tcggattta atcaaagaa ttctggata aaaacggcc 
      cgttacggc cgatttgtg tga
    mRNA sequence
    • >TC015712 RA
      acatttaaa ttcaacaaa cgaatttat tgagtaagt 
      tactttctc gttcactat ttcgtattt tcattttcg 
      gacttttca atcagtaga cgcgccacc gacaaaaat 
      gacacttat cggaattat gagcaccat tacggcccc 
      tttattggg cctgggagc cacggcagc ttccaattt 
      agccgtttt tatcccagc tttagcagc agctatcgc 
      ctatttcca gtacgacct gcttgaccc cgagtcaag 
      acctatcga tgtttcgac tgacgaatt attggaacg 
      gtacgactt tatagtggt tggggcggg atcagcagg 
      ggcggttgt ggcaaaccg acttagtga gatagaaca 
      atggaatgt gctcctgct ggaagcagg aggcgacga 
      gatggaaat ttcggacgt cccgctcat ggccgccta 
      cctccagct atcgcaaat cgactggaa gtacaaaag 
      cgagcccca aggccaagc ctgtctcgc catgaaaaa 
      cggccgttg taactggcc ccggggcaa agtcatcgg 
      gggctcctc cgtcctcaa ctacatgtt gtacctccg 
      cggcaataa aaaagacta cgacatttg ggaatccct 
      gggtaaccc gggctgggg cagccagga cgccctcta 
      ctacttcaa aaaatcgga agacaacca aaatcccta 
      cttgtcccg caccccgta ccacgccac tgggggcta 
      cttgacggt gtccgaagc cccctacca cacccccct 
      ggtggcggc gttcgtgga aggcggccg ccaactggg 
      ctacgccaa ccgggacat caacggcga gcaccaatc 
      cgggttcat gatggcgca aggtaccac ccggagggg 
      gtcgcgctg ctcgaccgg aaaagcatt cctgcgtcc 
      ggtacgcct ccggaagaa cctccacgt ggccatgca 
      cgcccacgt gaccaaggt catggtcga ccccaccag 
      taaagtggc cttcggggt cgagttcgt gagggataa 
      gaagttgta ccggattcg ggccaccaa agaggtggt 
      actttcggc aggcgcggt caattcgcc ccaactgct 
      catgttgtc agggatcgg gcctaagga ggatctgga 
      acggctcaa aatcccact tgtgcagga tttaaaggt 
      tgggcacaa cctacagga ccatgtggg gttaggggg 
      attgacgtt tctgatcaa tcggccaca ctcgatcct 
      cctcaatcg gctgtattc ggttagttc gctaatgca 
      gtacgcgat tttcggggg cggcccctt gaccatcat 
      gggtggggt ggaggggtt ggcgttcgt taacacgaa 
      atatgtcaa cgcttcgga cgatttccc cgatattga 
      gctacattt catctcggg ttcgacgca ttctgatgg 
      tggtaccca gcttaggaa agcccatgg gctcactga 
      cgcgttcta tgagcgggt ttttgggcc cattgccga 
      taaggacgc ctggagtgt tatcccaat gctgctaag 
      gcccaaatc acgcggttt cattaaatt gcggagcaa 
      aaacccact ggactaccc tttaatcta ccccaacta 
      cttcaaaga tgatttcga tatgaaaac tttgattga 
      aggagcgaa actaagcgt ggccttgag ccaaacccc 
      ggcgttcaa gtactacaa aagtaccct ccacaaatt 
      cccggactg tgccggttt caaagacta ctcggatga 
      gttttacga gtgtatgat ccgcctcta cactgtcac 
      aatttacca tccggtcgg aacgtgcaa aatgggccc 
      ttattggga ccaggaggc cgtcgtgga cccacaatt 
      gcgcgttta cggcatcaa gggcctaag ggtgatcga 
      tgcttcaat aatgccaaa cctagtgag tggcaacac 
      gaacgcccc tgccataat gatcgggga aaaagggtc 
      ggatttaat caaagaatt ctggataaa aacggcccg 
      ttacggccg atttgtgtg atactatct ataataata 
      aattataaa agtacca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY