Status
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Gene information
General information
TC015712 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5711732..5713919
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015712
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05880

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    remarks: later than schedule, hours 48
    Pupal morphology
    remarks: later than schedule, hours 48
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence broader - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: dead larva
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     antenna antennal flagellum size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    urogomphi orientation irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015712 RA
      MTLIGIMST ITAPLLGLG ATAASNLAV FIPALAAAI 
AYFQYDLLD PESRPIDVS TDELLERYD FIVVGAGSA 
GAVVANRLS EIEQWNVLL LEAGGDEME ISDVPLMAA 
YLQLSQIDW KYKSEPQGQ ACLAMKNGR CNWPRGKVI 
GGSSVLNYM LYLRGNKKD YDIWESLGN PGWGSQDAL 
YYFKKSEDN QNPYLSRTP YHATGGYLT VSEAPYHTP 
LVAAFVEGG RQLGYANRD INGEHQSGF MMAQGTTRR 
GSRCSTGKA FLRPVRLRK NLHVAMHAH VTKVMVDPT 
SKVAFGVEF VRDKKLYRI RATKEVVLS AGAVNSPQL 
LMLSGIGPK EDLERLKIP LVQDLKVGH NLQDHVGLG 
GLTFLINRP HSILLNRLY SVSSLMQYA IFGGGPLTI 
MGGVEGLAF VNTKYVNAS DDFPDIELH FISGSTHSD 
GGTQLRKAH GLTDAFYER VFGPIADKD AWSVIPMLL 
RPKSRGFIK LRSKNPLDY PLIYPNYFK DDFDMKTLI 
EGAKLSVAL SQTPAFKYY KSTLHKFPD CAGFKDYSD 
EFYECMIRL YTVTIYHPV GTCKMGPYW DQEAVVDPQ 
LRVYGIKGL RVIDASIMP NLVSGNTNA PAIMIGEKG 
SDLIKEFWI KTARYGRFV
    CDS sequence
    • >TC015712 RA
      atgacactt atcggaatt atgagcacc attacggcc 
cctttattg ggcctggga gccacggca gcttccaat 
ttagccgtt tttatccca gctttagca gcagctatc 
gcctatttc cagtacgac ctgcttgac cccgagtca 
agacctatc gatgtttcg actgacgaa ttattggaa 
cggtacgac tttatagtg gttggggcg ggatcagca 
ggggcggtt gtggcaaac cgacttagt gagatagaa 
caatggaat gtgctcctg ctggaagca ggaggcgac 
gagatggaa atttcggac gtcccgctc atggccgcc 
tacctccag ctatcgcaa atcgactgg aagtacaaa 
agcgagccc caaggccaa gcctgtctc gccatgaaa 
aacggccgt tgtaactgg ccccggggc aaagtcatc 
gggggctcc tccgtcctc aactacatg ttgtacctc 
cgcggcaat aaaaaagac tacgacatt tgggaatcc 
ctgggtaac ccgggctgg ggcagccag gacgccctc 
tactacttc aaaaaatcg gaagacaac caaaatccc 
tacttgtcc cgcaccccg taccacgcc actgggggc 
tacttgacg gtgtccgaa gccccctac cacaccccc 
ctggtggcg gcgttcgtg gaaggcggc cgccaactg 
ggctacgcc aaccgggac atcaacggc gagcaccaa 
tccgggttc atgatggcg caaggtacc acccggagg 
gggtcgcgc tgctcgacc ggaaaagca ttcctgcgt 
ccggtacgc ctccggaag aacctccac gtggccatg 
cacgcccac gtgaccaag gtcatggtc gaccccacc 
agtaaagtg gccttcggg gtcgagttc gtgagggat 
aagaagttg taccggatt cgggccacc aaagaggtg 
gtactttcg gcaggcgcg gtcaattcg ccccaactg 
ctcatgttg tcagggatc gggcctaag gaggatctg 
gaacggctc aaaatccca cttgtgcag gatttaaag 
gttgggcac aacctacag gaccatgtg gggttaggg 
ggattgacg tttctgatc aatcggcca cactcgatc 
ctcctcaat cggctgtat tcggttagt tcgctaatg 
cagtacgcg attttcggg ggcggcccc ttgaccatc 
atgggtggg gtggagggg ttggcgttc gttaacacg 
aaatatgtc aacgcttcg gacgatttc cccgatatt 
gagctacat ttcatctcg ggttcgacg cattctgat 
ggtggtacc cagcttagg aaagcccat gggctcact 
gacgcgttc tatgagcgg gtttttggg cccattgcc 
gataaggac gcctggagt gttatccca atgctgcta 
aggcccaaa tcacgcggt ttcattaaa ttgcggagc 
aaaaaccca ctggactac cctttaatc taccccaac 
tacttcaaa gatgatttc gatatgaaa actttgatt 
gaaggagcg aaactaagc gtggccttg agccaaacc 
ccggcgttc aagtactac aaaagtacc ctccacaaa 
ttcccggac tgtgccggt ttcaaagac tactcggat 
gagttttac gagtgtatg atccgcctc tacactgtc 
acaatttac catccggtc ggaacgtgc aaaatgggc 
ccttattgg gaccaggag gccgtcgtg gacccacaa 
ttgcgcgtt tacggcatc aagggccta agggtgatc 
gatgcttca ataatgcca aacctagtg agtggcaac 
acgaacgcc cctgccata atgatcggg gaaaaaggg 
tcggattta atcaaagaa ttctggata aaaacggcc 
cgttacggc cgatttgtg tga
    mRNA sequence
    • >TC015712 RA
      acatttaaa ttcaacaaa cgaatttat tgagtaagt 
tactttctc gttcactat ttcgtattt tcattttcg 
gacttttca atcagtaga cgcgccacc gacaaaaat 
gacacttat cggaattat gagcaccat tacggcccc 
tttattggg cctgggagc cacggcagc ttccaattt 
agccgtttt tatcccagc tttagcagc agctatcgc 
ctatttcca gtacgacct gcttgaccc cgagtcaag 
acctatcga tgtttcgac tgacgaatt attggaacg 
gtacgactt tatagtggt tggggcggg atcagcagg 
ggcggttgt ggcaaaccg acttagtga gatagaaca 
atggaatgt gctcctgct ggaagcagg aggcgacga 
gatggaaat ttcggacgt cccgctcat ggccgccta 
cctccagct atcgcaaat cgactggaa gtacaaaag 
cgagcccca aggccaagc ctgtctcgc catgaaaaa 
cggccgttg taactggcc ccggggcaa agtcatcgg 
gggctcctc cgtcctcaa ctacatgtt gtacctccg 
cggcaataa aaaagacta cgacatttg ggaatccct 
gggtaaccc gggctgggg cagccagga cgccctcta 
ctacttcaa aaaatcgga agacaacca aaatcccta 
cttgtcccg caccccgta ccacgccac tgggggcta 
cttgacggt gtccgaagc cccctacca cacccccct 
ggtggcggc gttcgtgga aggcggccg ccaactggg 
ctacgccaa ccgggacat caacggcga gcaccaatc 
cgggttcat gatggcgca aggtaccac ccggagggg 
gtcgcgctg ctcgaccgg aaaagcatt cctgcgtcc 
ggtacgcct ccggaagaa cctccacgt ggccatgca 
cgcccacgt gaccaaggt catggtcga ccccaccag 
taaagtggc cttcggggt cgagttcgt gagggataa 
gaagttgta ccggattcg ggccaccaa agaggtggt 
actttcggc aggcgcggt caattcgcc ccaactgct 
catgttgtc agggatcgg gcctaagga ggatctgga 
acggctcaa aatcccact tgtgcagga tttaaaggt 
tgggcacaa cctacagga ccatgtggg gttaggggg 
attgacgtt tctgatcaa tcggccaca ctcgatcct 
cctcaatcg gctgtattc ggttagttc gctaatgca 
gtacgcgat tttcggggg cggcccctt gaccatcat 
gggtggggt ggaggggtt ggcgttcgt taacacgaa 
atatgtcaa cgcttcgga cgatttccc cgatattga 
gctacattt catctcggg ttcgacgca ttctgatgg 
tggtaccca gcttaggaa agcccatgg gctcactga 
cgcgttcta tgagcgggt ttttgggcc cattgccga 
taaggacgc ctggagtgt tatcccaat gctgctaag 
gcccaaatc acgcggttt cattaaatt gcggagcaa 
aaacccact ggactaccc tttaatcta ccccaacta 
cttcaaaga tgatttcga tatgaaaac tttgattga 
aggagcgaa actaagcgt ggccttgag ccaaacccc 
ggcgttcaa gtactacaa aagtaccct ccacaaatt 
cccggactg tgccggttt caaagacta ctcggatga 
gttttacga gtgtatgat ccgcctcta cactgtcac 
aatttacca tccggtcgg aacgtgcaa aatgggccc 
ttattggga ccaggaggc cgtcgtgga cccacaatt 
gcgcgttta cggcatcaa gggcctaag ggtgatcga 
tgcttcaat aatgccaaa cctagtgag tggcaacac 
gaacgcccc tgccataat gatcgggga aaaagggtc 
ggatttaat caaagaatt ctggataaa aacggcccg 
ttacggccg atttgtgtg atactatct ataataata 
aattataaa agtacca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY