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Gene information
General information
TC015705 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5673012..5674474
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015705
    iBeetle screen iB_05878

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015705 RA
      MSHYLQPIS EAQKIEILK TFGRTPETV KSDIEAIEE 
      WLKKQPHLQ KIPTDIPIE NFLHLAKFS VEGTKRLID 
      DFYTIRSQL PRIFNTKEV NPNSEILAK YADISYFIP 
      LPKLTSDLS RVIVCKFIE DSELFNPIY SYIWSVNVN 
      VIRLLDDFA VNNIVIYDL EHCTLRHLA KITPTMLKI 
      FPEIRKKIL NRSVKSVNL VNCSSLTDY FLRFFKPLA 
      SQKVVERIC VHNDLQSLH QVVPKEVLP KDYGGEEKS 
      LKELNDLLK CKVRESRDV FDNLDNFTV DETLRPAKL 
      VNDDILGVY GNFKRLAID
    CDS sequence
    • >TC015705 RA
      atgtcgcac tacttacaa ccaatttca gaagcccaa 
      aaaatcgaa attttgaaa acctttggc cgaacccct 
      gaaacggtc aagagtgac attgaagcg atcgaagaa 
      tggctcaaa aaacagcca cacttgcaa aaaatcccc 
      actgatatc ccgatcgaa aactttcta catctggcc 
      aaattcagt gtcgaagga acgaagaga ctaatcgat 
      gacttttac acaattcgc tcgcaactg ccgcgaatt 
      ttcaacacg aaagaagtg aatccgaat tcggaaatt 
      ctggccaaa tatgccgac atcagttat tttatcccg 
      ttaccgaaa ctaacaagt gacttgagc cgagtcatt 
      gtgtgtaaa ttcatcgaa gactcggag ctttttaac 
      ccgatttat tcctacatt tggagtgtc aatgtaaat 
      gtaattcga ttattggac gactttgct gttaacaac 
      atcgtgatt tatgatctc gagcattgc actttgaga 
      cacttggcc aaaatcacg ccaacaatg cttaagatt 
      ttccccgag attcgcaaa aaaatttta aacaggtcg 
      gtcaaatcg gtcaatttg gtcaattgt tcctcactc 
      actgactac ttcctccgt ttttttaaa ccactagca 
      agtcagaaa gtcgtggaa cggatttgt gtgcataac 
      gacttgcaa agtttgcac caagtcgtg cctaaggaa 
      gtcctgccg aaggattac ggcggcgag gagaaatct 
      ttgaaggaa ttgaacgac ttgcttaaa tgcaaagtt 
      agggagagt cgggacgtt ttcgacaac ttggacaac 
      ttcacagtt gacgaaacg ctacgcccg gccaagcta 
      gtcaacgac gacatcctg ggtgtttat ggcaatttt 
      aaacgactt gctatcgat tga
    mRNA sequence
    • >TC015705 RA
      cataaaaaa acagccatg tcgcactac ttacaacca 
      atttcagaa gcccaaaaa atcgaaatt ttgaaaacc 
      tttggccga acccctgaa acggtcaag agtgacatt 
      gaagcgatc gaagaatgg ctcaaaaaa cagccacac 
      ttgcaaaaa atccccact gatatcccg atcgaaaac 
      tttctacat ctggccaaa ttcagtgtc gaaggaacg 
      aagagacta atcgatgac ttttacaca attcgctcg 
      caactgccg cgaattttc aacacgaaa gaagtgaat 
      ccgaattcg gaaattctg gccaaatat gccgacatc 
      agttatttt atcccgtta ccgaaacta acaagtgac 
      ttgagccga gtcattgtg tgtaaattc atcgaagac 
      tcggagctt tttaacccg atttattcc tacatttgg 
      agtgtcaat gtaaatgta attcgatta ttggacgac 
      tttgctgtt aacaacatc gtgatttat gatctcgag 
      cattgcact ttgagacac ttggccaaa atcacgcca 
      acaatgctt aagattttc cccgagatt cgcaaaaaa 
      attttaaac aggtcggtc aaatcggtc aatttggtc 
      aattgttcc tcactcact gactacttc ctccgtttt 
      tttaaacca ctagcaagt cagaaagtc gtggaacgg 
      atttgtgtg cataacgac ttgcaaagt ttgcaccaa 
      gtcgtgcct aaggaagtc ctgccgaag gattacggc 
      ggcgaggag aaatctttg aaggaattg aacgacttg 
      cttaaatgc aaagttagg gagagtcgg gacgttttc 
      gacaacttg gacaacttc acagttgac gaaacgcta 
      cgcccggcc aagctagtc aacgacgac atcctgggt 
      gtttatggc aattttaaa cgacttgct atcgattga 
      ataaaaatt ttggtttat tttattcat gttttacgc 
      cccactaga cgtttcctc gtccttttt ccccaattt 
      tccccgagt caggccata gatcagggc cgtatcgac 
      tcataatca aatcttaca caaaataag ttacttaga 
      taccgtaaa atacatcgt aaaatcgag a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY