Status
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Gene information
General information
TC015696 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5315043..5326621
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015696
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05876

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015696 RA
      MEHIRADNS LNQINQFIK SIKDKGLLV ERDLGSFHK 
      SLYFIKVHG PHKVLVRYA KIYNITLTC QNPYYKYQE 
      TTWFNFLTT EVTKPNPES KVYQRAEKT LSGNRPTEI 
      TTAERSLIV LEIMKRVSF VVADDEFTF KVMLEKQIF 
      VGAFPIHDG DCEWTEEGF LTDRQLLFQ YWTNVKYLF 
      KDQPVHLIE KYYGPKIAF YFALVNFYT RMLVVPTVI 
      GVITLVTGI ALLFKERFI EDEVCNSKV TICSDQSLQ 
      TACNVMKLA YIIDNPVTI IYVILCSFW GTIFWNMWQ 
      RRQAMLRLR WNLTGLKLD RTMRRQFQE SAKTRKSSI 
      TGEIEYYVP PHKKVLSAV FSYVIIFLM ITVFFLIVF 
      LMVQYRFFL NKSLDYDET NSANEIELF TSSVITTVI 
      IMGIDQCTP FLMKTYSKI VHFLTFLRQ PRTQTQYNQ 
      MYLHMTFIL IFANHFSPL FYLAYIKGF LHAYPGDLS 
      KFSMIRRIQ MDFCKPSGC LVDLGMQLI TLMVVKTAM 
      KCFQDFVAH TFSSSDTVI KDPAVGQWE RDYGKKQLT 
      DSVINQLYT ELVLQYSFI TFFNASLPI IGLVALTHN 
      LFKTQYDSY KFVKFYRRP SHERVADIQ GWNHILKAV 
      TIFGTISNA FVVAFRTDF IIRLLYRKA HDGSLKGYV 
      NSTLSKFAV KDYQRGELL IDHKNESFC FYRGFRHSP 
      EHENKYEPT LDHWYYLAV KSLYFIVLE NVTLILSLI 
      LGYLIPEIP RKVKVKLEY DAKIINEAR EARMELEKW
    CDS sequence
    • >TC015696 RA
      atggaacac attcgagct gacaactcc ctaaaccaa 
      atcaaccag ttcattaaa agcatcaaa gataaagga 
      ttgttggtt gaaagagac ctgggaagt tttcacaag 
      tctttgtac ttcatcaaa gttcacgga ccgcacaaa 
      gtcttggta cgatatgcc aaaatttac aatatcacc 
      ctcacatgc caaaacccc tactacaag taccaagaa 
      accacttgg ttcaacttt ctcacaact gaagtgacg 
      aaaccaaac cctgaaagc aaggtttac cagagagca 
      gaaaaaacc ctctctggt aacagaccg actgaaata 
      acgaccgca gaacgcagt cttatagtt ttggaaata 
      atgaaacgt gtttcattc gtggttgcc gacgacgag 
      ttcactttc aaagtaatg ctggaaaag caaattttc 
      gtgggagcg tttccgatc catgatggt gattgcgaa 
      tggacggag gaagggttt ttgacagat cgacagctc 
      ctattccag tattggaca aacgttaag tacttgttt 
      aaggaccaa cctgtacat ttgattgag aagtactac 
      gggcctaag attgctttc tacttcgca ctggttaat 
      ttttataca cgaatgttg gtcgtgccg acggttata 
      ggggttata actttggtt acagggatt gcgttgctt 
      tttaaggaa agattcatt gaggacgaa gtttgtaac 
      agcaaggtc acaatttgt tcggaccaa agcctccaa 
      actgcttgc aatgtaatg aaattagcc tacattata 
      gacaaccct gtgacgata atctacgtt attttgtgt 
      tcgttctgg ggcacaatt ttctggaac atgtggcag 
      aggcgtcaa gcaatgctt cgcttaaga tggaatttg 
      actggactt aaacttgac cgcactatg agacgccag 
      tttcaggaa agtgcaaaa acgcgaaaa tcgtcaatt 
      accggggaa attgaatat tatgttccg ccgcataag 
      aaagtactc tcagctgtg ttttcctac gttattatt 
      tttttaatg ataactgta ttttttctc atcgtgttt 
      ttgatggtt cagtatagg tttttcctg aataagtcg 
      ctagattac gatgagaca aacagtgcc aacgagatt 
      gaattgttc acttcgtcg gtcataaca acagtcata 
      attatgggt attgatcag tgcactcct ttcctgatg 
      aaaacctat tccaaaata gtccatttt ttgaccttc 
      cttcgccag cctcgcact caaacccaa tacaaccaa 
      atgtacttg cacatgact ttcatttta atttttgca 
      aatcatttc tccccactt ttctatctc gcatacatc 
      aaaggattc cttcacgca taccctgga gacttatcg 
      aaattttca atgattaga agaatccag atggacttt 
      tgtaaacca tccggttgt cttgtagat ctaggaatg 
      cagttgatc acacttatg gtcgtcaaa acagcaatg 
      aagtgcttc caagacttt gtggcacac acattttct 
      tcctctgat acagtgatt aaagaccca gctgtggga 
      cagtgggag agggattat ggcaagaaa caattaact 
      gattctgtt attaaccaa ttgtataca gaattggtc 
      ttgcagtac agttttata acgtttttt aacgcctct 
      ttgcccatc atcggactt gtggcttta acccacaat 
      ttgttcaaa actcaatac gattcgtac aaatttgtg 
      aaattttat cggcgaccg agtcacgag agagttgcc 
      gatatacaa ggatggaat cacatactt aaagccgtc 
      acaattttt ggaacaata tcaaatgcg tttgtggtg 
      gctttcagg accgatttt atcattcgg cttttgtac 
      agaaaggca cacgatggt agtttaaaa ggctacgtc 
      aatagtaca ctttctaaa tttgcagtt aaagactat 
      cagcgtgga gagttgtta atagaccat aaaaatgag 
      tcgttttgt ttttatcgt ggctttaga cactcgcct 
      gagcatgaa aataaatac gagccgact ttggaccat 
      tggtattat ttggcagta aagtcgctc tattttatt 
      gttttagaa aatgttacc ttaattttg tcactaatt 
      ttgggttat ctcataccg gaaatacca cggaaagtt 
      aaagtgaag ctggaatat gacgcaaaa attataaat 
      gaagcaagg gaagcccgg atggaattg gaaaagtgg 
      tga
    mRNA sequence
    • >TC015696 RA
      gtgtacgca aaaatggaa cacattcga gctgacaac 
      tccctaaac caaatcaac cagttcatt aaaagcatc 
      aaagataaa ggattgttg gttgaaaga gacctggga 
      agttttcac aagtctttg tacttcatc aaagttcac 
      ggaccgcac aaagtcttg gtacgatat gccaaaatt 
      tacaatatc accctcaca tgccaaaac ccctactac 
      aagtaccaa gaaaccact tggttcaac tttctcaca 
      actgaagtg acgaaacca aaccctgaa agcaaggtt 
      taccagaga gcagaaaaa accctctct ggtaacaga 
      ccgactgaa ataacgacc gcagaacgc agtcttata 
      gttttggaa ataatgaaa cgtgtttca ttcgtggtt 
      gccgacgac gagttcact ttcaaagta atgctggaa 
      aagcaaatt ttcgtggga gcgtttccg atccatgat 
      ggtgattgc gaatggacg gaggaaggg tttttgaca 
      gatcgacag ctcctattc cagtattgg acaaacgtt 
      aagtacttg tttaaggac caacctgta catttgatt 
      gagaagtac tacgggcct aagattgct ttctacttc 
      gcactggtt aatttttat acacgaatg ttggtcgtg 
      ccgacggtt ataggggtt ataactttg gttacaggg 
      attgcgttg ctttttaag gaaagattc attgaggac 
      gaagtttgt aacagcaag gtcacaatt tgttcggac 
      caaagcctc caaactgct tgcaatgta atgaaatta 
      gcctacatt atagacaac cctgtgacg ataatctac 
      gttattttg tgttcgttc tggggcaca attttctgg 
      aacatgtgg cagaggcgt caagcaatg cttcgctta 
      agatggaat ttgactgga cttaaactt gaccgcact 
      atgagacgc cagtttcag gaaagtgca aaaacgcga 
      aaatcgtca attaccggg gaaattgaa tattatgtt 
      ccgccgcat aagaaagta ctctcagct gtgttttcc 
      tacgttatt attttttta atgataact gtatttttt 
      ctcatcgtg tttttgatg gttcagtat aggtttttc 
      ctgaataag tcgctagat tacgatgag acaaacagt 
      gccaacgag attgaattg ttcacttcg tcggtcata 
      acaacagtc ataattatg ggtattgat cagtgcact 
      cctttcctg atgaaaacc tattccaaa atagtccat 
      tttttgacc ttccttcgc cagcctcgc actcaaacc 
      caatacaac caaatgtac ttgcacatg actttcatt 
      ttaattttt gcaaatcat ttctcccca cttttctat 
      ctcgcatac atcaaagga ttccttcac gcataccct 
      ggagactta tcgaaattt tcaatgatt agaagaatc 
      cagatggac ttttgtaaa ccatccggt tgtcttgta 
      gatctagga atgcagttg atcacactt atggtcgtc 
      aaaacagca atgaagtgc ttccaagac tttgtggca 
      cacacattt tcttcctct gatacagtg attaaagac 
      ccagctgtg ggacagtgg gagagggat tatggcaag 
      aaacaatta actgattct gttattaac caattgtat 
      acagaattg gtcttgcag tacagtttt ataacgttt 
      tttaacgcc tctttgccc atcatcgga cttgtggct 
      ttaacccac aatttgttc aaaactcaa tacgattcg 
      tacaaattt gtgaaattt tatcggcga ccgagtcac 
      gagagagtt gccgatata caaggatgg aatcacata 
      cttaaagcc gtcacaatt tttggaaca atatcaaat 
      gcgtttgtg gtggctttc aggaccgat tttatcatt 
      cggcttttg tacagaaag gcacacgat ggtagttta 
      aaaggctac gtcaatagt acactttct aaatttgca 
      gttaaagac tatcagcgt ggagagttg ttaatagac 
      cataaaaat gagtcgttt tgtttttat cgtggcttt 
      agacactcg cctgagcat gaaaataaa tacgagccg 
      actttggac cattggtat tatttggca gtaaagtcg 
      ctctatttt attgtttta gaaaatgtt accttaatt 
      ttgtcacta attttgggt tatctcata ccggaaata 
      ccacggaaa gttaaagtg aagctggaa tatgacgca 
      aaaattata aatgaagca agggaagcc cggatggaa 
      ttggaaaag tggtgacta ctttataaa ggtgaagtg 
      taaaaatgg tggatgcgc cgggcgaga caggtgttt 
      ttttttctt tcctagtaa attaacata gaaactgca 
      aaatatatg atggtacct gtc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY