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Gene information
General information
TC033427
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015695
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5266858..5278052
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033427
    iBeetle screen iB_05875

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033427 RA
      LLLKYWANF SLWYKEQPL NLIEKYYGT EVAFYFAWL 
      GFYNMMLIP ASIVGVICF IISIINVKT IRYDTIEDI 
      CSSELKMCP RCSFGHSCV SQPLKLECE AASVFAVFD 
      NYVTIFFAV FMSFWATLF MNLWTRFEN VLKIRWNVT 
      TSTIVAKTQ TRLPYKEKA THKRISSVT GKLEHYTPT 
      RIQIAYYFL SFGACLLLV GVVLLAVLG VIFYRIVMA 
      ALIVMSNSL FLRSYTALF EACTSSLLQ VIFIKIYGK 
      FYAPLSEWL TNMENPRTQ VEFDNSVVH KRYILGFAN 
      NYASLFYMA FFKSRFYSP SHTGVISLK TDLCPSSGC 
      VMPLCVQLF VLMLLKSMF GNILTLVVP IVTRRFRKT 
      VVTNAAAPQ WEKEFGLYP AGRYLLTTE FMEMIIQYG 
      FVTFFVAAF PLAPLCALI NNFMELRLD AFKLVTRYR 
      RPVPKKMSG IGPWKKLLG TITHLSVAT NAFVLSFTT 
      DVVPREIYR FRHKTLKGY VESTLSIYD MKDNKRLEA 
      SDLEGMFME SNSTWCYYR ASRYPPDHP KKYKLTNAF 
      WYEIGLRLF CVIVFEHLI MLTNGILAY FIPPVPKKI 
      KRSIYYENR KHRNQVLDA MHEKVNAMR RKDVKPKTK 
      IIREE
    CDS sequence
    • >TC033427 RA
      ttgttgctg aaatactgg gcgaacttc tccctgtgg 
      tacaaagag caaccccta aatctcatc gaaaaatac 
      tacggaacg gaagttgca ttctacttt gcttggctt 
      ggattttac aacatgatg ctaatccct gcatcaatt 
      gtcggcgta atttgtttc atcatcagt ataatcaac 
      gtgaaaact attcgttac gatacaata gaagatatc 
      tgctcaagc gaactcaaa atgtgtcca agatgttca 
      tttggccac agttgtgtt tcacaaccg ttgaaactc 
      gaatgtgaa gctgcttca gtgtttgcc gtttttgat 
      aattacgtc acgatcttt tttgccgta tttatgtct 
      ttttgggca acattgttt atgaatttg tggacgcga 
      ttcgaaaac gttttaaaa atcagatgg aacgttacc 
      acgtcaact attgttgcc aaaacacaa acaagactg 
      ccatataaa gaaaaggcc actcacaaa agaatctct 
      tcagtcacc ggaaaattg gagcattac acacccacc 
      cgaattcaa attgcatat tactttctg tcttttggc 
      gcttgttta cttttggtc ggtgttgtg cttttggca 
      gtgcttggt gtaattttc tacagaata gtgatggca 
      gctttgatc gtaatgagc aattcctta ttcctgcga 
      agttatact gcattgttt gaagcttgc acaagctcg 
      ttgctacaa gtgattttt atcaaaatt tatggaaag 
      ttttatgca cctctttcc gaatggttg acgaacatg 
      gaaaatcca agaacgcag gttgagttt gataattca 
      gtcgtgcac aagcgatac attttgggt tttgcgaat 
      aattacgcc tcgttgttc tatatggcg ttttttaaa 
      agtcggttt tattcaccg tctcatact ggagttatt 
      agtttgaaa acggatctt tgtccttcg agtggttgt 
      gtgatgcct ctctgtgtc caactcttt gttttgatg 
      cttctgaaa tcaatgttt ggaaatatt ttaacactt 
      gttgttccg atagtcact cgacgcttt agaaaaact 
      gtggtaaca aacgcagct gcgccccag tgggagaaa 
      gaatttggt ttatatcca gctggtcgt tacttactc 
      actaccgaa tttatggaa atgatcatt caatacggt 
      tttgtcacg ttttttgtg gcagctttt ccccttgca 
      ccactttgc gctttgatt aataatttt atggaattg 
      cgtttggat gcatttaag ttggtaaca agatacaga 
      agaccggtg cctaaaaaa atgtcgggg atcggtccc 
      tggaagaaa cttttggga acgatcact cacttgagt 
      gttgcgact aatgccttt gtcttgtcg ttcactacc 
      gatgttgtg ccacgggag atttacaga tttcggcac 
      aaaacactg aaaggttac gttgaatcg accttgtcc 
      atttacgat atgaaagac aataaaaga ctggaggcc 
      agtgatctc gaaggcatg ttcatggaa agcaattcc 
      acctggtgc tactacagg gcgtctcgt tatccgcca 
      gatcacccg aaaaaatac aaactaacc aatgcgttt 
      tggtacgaa attgggctt cgactcttc tgtgtcata 
      gtttttgag catctgatt atgttgact aatggtatt 
      ttggcatat tttataccg ccagttccc aaaaaaatc 
      aaacgctcg atttattac gaaaatagg aaacatcga 
      aatcaagtt ttggacgca atgcacgaa aaggtgaat 
      gcgatgcga cggaaggac gtaaaaccg aagaccaaa 
      attattaga gaagagtaa
    mRNA sequence
    • >TC033427 RA
      ttgttgctg aaatactgg gcgaacttc tccctgtgg 
      tacaaagag caaccccta aatctcatc gaaaaatac 
      tacggaacg gaagttgca ttctacttt gcttggctt 
      ggattttac aacatgatg ctaatccct gcatcaatt 
      gtcggcgta atttgtttc atcatcagt ataatcaac 
      gtgaaaact attcgttac gatacaata gaagatatc 
      tgctcaagc gaactcaaa atgtgtcca agatgttca 
      tttggccac agttgtgtt tcacaaccg ttgaaactc 
      gaatgtgaa gctgcttca gtgtttgcc gtttttgat 
      aattacgtc acgatcttt tttgccgta tttatgtct 
      ttttgggca acattgttt atgaatttg tggacgcga 
      ttcgaaaac gttttaaaa atcagatgg aacgttacc 
      acgtcaact attgttgcc aaaacacaa acaagactg 
      ccatataaa gaaaaggcc actcacaaa agaatctct 
      tcagtcacc ggaaaattg gagcattac acacccacc 
      cgaattcaa attgcatat tactttctg tcttttggc 
      gcttgttta cttttggtc ggtgttgtg cttttggca 
      gtgcttggt gtaattttc tacagaata gtgatggca 
      gctttgatc gtaatgagc aattcctta ttcctgcga 
      agttatact gcattgttt gaagcttgc acaagctcg 
      ttgctacaa gtgattttt atcaaaatt tatggaaag 
      ttttatgca cctctttcc gaatggttg acgaacatg 
      gaaaatcca agaacgcag gttgagttt gataattca 
      gtcgtgcac aagcgatac attttgggt tttgcgaat 
      aattacgcc tcgttgttc tatatggcg ttttttaaa 
      agtcggttt tattcaccg tctcatact ggagttatt 
      agtttgaaa acggatctt tgtccttcg agtggttgt 
      gtgatgcct ctctgtgtc caactcttt gttttgatg 
      cttctgaaa tcaatgttt ggaaatatt ttaacactt 
      gttgttccg atagtcact cgacgcttt agaaaaact 
      gtggtaaca aacgcagct gcgccccag tgggagaaa 
      gaatttggt ttatatcca gctggtcgt tacttactc 
      actaccgaa tttatggaa atgatcatt caatacggt 
      tttgtcacg ttttttgtg gcagctttt ccccttgca 
      ccactttgc gctttgatt aataatttt atggaattg 
      cgtttggat gcatttaag ttggtaaca agatacaga 
      agaccggtg cctaaaaaa atgtcgggg atcggtccc 
      tggaagaaa cttttggga acgatcact cacttgagt 
      gttgcgact aatgccttt gtcttgtcg ttcactacc 
      gatgttgtg ccacgggag atttacaga tttcggcac 
      aaaacactg aaaggttac gttgaatcg accttgtcc 
      atttacgat atgaaagac aataaaaga ctggaggcc 
      agtgatctc gaaggcatg ttcatggaa agcaattcc 
      acctggtgc tactacagg gcgtctcgt tatccgcca 
      gatcacccg aaaaaatac aaactaacc aatgcgttt 
      tggtacgaa attgggctt cgactcttc tgtgtcata 
      gtttttgag catctgatt atgttgact aatggtatt 
      ttggcatat tttataccg ccagttccc aaaaaaatc 
      aaacgctcg atttattac gaaaatagg aaacatcga 
      aatcaagtt ttggacgca atgcacgaa aaggtgaat 
      gcgatgcga cggaaggac gtaaaaccg aagaccaaa 
      attattaga gaagagtaa attagtttg tgttaatgt 
      tgttacttt gtgtttcat aataaaagt tatgggttc 
      ag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY