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Gene information
General information
TC033427
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015695 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5266858..5278052
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033427
    iBeetle screen iB_05875

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033427 RA
      LLLKYWANF SLWYKEQPL NLIEKYYGT EVAFYFAWL 
GFYNMMLIP ASIVGVICF IISIINVKT IRYDTIEDI 
CSSELKMCP RCSFGHSCV SQPLKLECE AASVFAVFD 
NYVTIFFAV FMSFWATLF MNLWTRFEN VLKIRWNVT 
TSTIVAKTQ TRLPYKEKA THKRISSVT GKLEHYTPT 
RIQIAYYFL SFGACLLLV GVVLLAVLG VIFYRIVMA 
ALIVMSNSL FLRSYTALF EACTSSLLQ VIFIKIYGK 
FYAPLSEWL TNMENPRTQ VEFDNSVVH KRYILGFAN 
NYASLFYMA FFKSRFYSP SHTGVISLK TDLCPSSGC 
VMPLCVQLF VLMLLKSMF GNILTLVVP IVTRRFRKT 
VVTNAAAPQ WEKEFGLYP AGRYLLTTE FMEMIIQYG 
FVTFFVAAF PLAPLCALI NNFMELRLD AFKLVTRYR 
RPVPKKMSG IGPWKKLLG TITHLSVAT NAFVLSFTT 
DVVPREIYR FRHKTLKGY VESTLSIYD MKDNKRLEA 
SDLEGMFME SNSTWCYYR ASRYPPDHP KKYKLTNAF 
WYEIGLRLF CVIVFEHLI MLTNGILAY FIPPVPKKI 
KRSIYYENR KHRNQVLDA MHEKVNAMR RKDVKPKTK 
IIREE
    CDS sequence
    • >TC033427 RA
      ttgttgctg aaatactgg gcgaacttc tccctgtgg 
tacaaagag caaccccta aatctcatc gaaaaatac 
tacggaacg gaagttgca ttctacttt gcttggctt 
ggattttac aacatgatg ctaatccct gcatcaatt 
gtcggcgta atttgtttc atcatcagt ataatcaac 
gtgaaaact attcgttac gatacaata gaagatatc 
tgctcaagc gaactcaaa atgtgtcca agatgttca 
tttggccac agttgtgtt tcacaaccg ttgaaactc 
gaatgtgaa gctgcttca gtgtttgcc gtttttgat 
aattacgtc acgatcttt tttgccgta tttatgtct 
ttttgggca acattgttt atgaatttg tggacgcga 
ttcgaaaac gttttaaaa atcagatgg aacgttacc 
acgtcaact attgttgcc aaaacacaa acaagactg 
ccatataaa gaaaaggcc actcacaaa agaatctct 
tcagtcacc ggaaaattg gagcattac acacccacc 
cgaattcaa attgcatat tactttctg tcttttggc 
gcttgttta cttttggtc ggtgttgtg cttttggca 
gtgcttggt gtaattttc tacagaata gtgatggca 
gctttgatc gtaatgagc aattcctta ttcctgcga 
agttatact gcattgttt gaagcttgc acaagctcg 
ttgctacaa gtgattttt atcaaaatt tatggaaag 
ttttatgca cctctttcc gaatggttg acgaacatg 
gaaaatcca agaacgcag gttgagttt gataattca 
gtcgtgcac aagcgatac attttgggt tttgcgaat 
aattacgcc tcgttgttc tatatggcg ttttttaaa 
agtcggttt tattcaccg tctcatact ggagttatt 
agtttgaaa acggatctt tgtccttcg agtggttgt 
gtgatgcct ctctgtgtc caactcttt gttttgatg 
cttctgaaa tcaatgttt ggaaatatt ttaacactt 
gttgttccg atagtcact cgacgcttt agaaaaact 
gtggtaaca aacgcagct gcgccccag tgggagaaa 
gaatttggt ttatatcca gctggtcgt tacttactc 
actaccgaa tttatggaa atgatcatt caatacggt 
tttgtcacg ttttttgtg gcagctttt ccccttgca 
ccactttgc gctttgatt aataatttt atggaattg 
cgtttggat gcatttaag ttggtaaca agatacaga 
agaccggtg cctaaaaaa atgtcgggg atcggtccc 
tggaagaaa cttttggga acgatcact cacttgagt 
gttgcgact aatgccttt gtcttgtcg ttcactacc 
gatgttgtg ccacgggag atttacaga tttcggcac 
aaaacactg aaaggttac gttgaatcg accttgtcc 
atttacgat atgaaagac aataaaaga ctggaggcc 
agtgatctc gaaggcatg ttcatggaa agcaattcc 
acctggtgc tactacagg gcgtctcgt tatccgcca 
gatcacccg aaaaaatac aaactaacc aatgcgttt 
tggtacgaa attgggctt cgactcttc tgtgtcata 
gtttttgag catctgatt atgttgact aatggtatt 
ttggcatat tttataccg ccagttccc aaaaaaatc 
aaacgctcg atttattac gaaaatagg aaacatcga 
aatcaagtt ttggacgca atgcacgaa aaggtgaat 
gcgatgcga cggaaggac gtaaaaccg aagaccaaa 
attattaga gaagagtaa
    mRNA sequence
    • >TC033427 RA
      ttgttgctg aaatactgg gcgaacttc tccctgtgg 
tacaaagag caaccccta aatctcatc gaaaaatac 
tacggaacg gaagttgca ttctacttt gcttggctt 
ggattttac aacatgatg ctaatccct gcatcaatt 
gtcggcgta atttgtttc atcatcagt ataatcaac 
gtgaaaact attcgttac gatacaata gaagatatc 
tgctcaagc gaactcaaa atgtgtcca agatgttca 
tttggccac agttgtgtt tcacaaccg ttgaaactc 
gaatgtgaa gctgcttca gtgtttgcc gtttttgat 
aattacgtc acgatcttt tttgccgta tttatgtct 
ttttgggca acattgttt atgaatttg tggacgcga 
ttcgaaaac gttttaaaa atcagatgg aacgttacc 
acgtcaact attgttgcc aaaacacaa acaagactg 
ccatataaa gaaaaggcc actcacaaa agaatctct 
tcagtcacc ggaaaattg gagcattac acacccacc 
cgaattcaa attgcatat tactttctg tcttttggc 
gcttgttta cttttggtc ggtgttgtg cttttggca 
gtgcttggt gtaattttc tacagaata gtgatggca 
gctttgatc gtaatgagc aattcctta ttcctgcga 
agttatact gcattgttt gaagcttgc acaagctcg 
ttgctacaa gtgattttt atcaaaatt tatggaaag 
ttttatgca cctctttcc gaatggttg acgaacatg 
gaaaatcca agaacgcag gttgagttt gataattca 
gtcgtgcac aagcgatac attttgggt tttgcgaat 
aattacgcc tcgttgttc tatatggcg ttttttaaa 
agtcggttt tattcaccg tctcatact ggagttatt 
agtttgaaa acggatctt tgtccttcg agtggttgt 
gtgatgcct ctctgtgtc caactcttt gttttgatg 
cttctgaaa tcaatgttt ggaaatatt ttaacactt 
gttgttccg atagtcact cgacgcttt agaaaaact 
gtggtaaca aacgcagct gcgccccag tgggagaaa 
gaatttggt ttatatcca gctggtcgt tacttactc 
actaccgaa tttatggaa atgatcatt caatacggt 
tttgtcacg ttttttgtg gcagctttt ccccttgca 
ccactttgc gctttgatt aataatttt atggaattg 
cgtttggat gcatttaag ttggtaaca agatacaga 
agaccggtg cctaaaaaa atgtcgggg atcggtccc 
tggaagaaa cttttggga acgatcact cacttgagt 
gttgcgact aatgccttt gtcttgtcg ttcactacc 
gatgttgtg ccacgggag atttacaga tttcggcac 
aaaacactg aaaggttac gttgaatcg accttgtcc 
atttacgat atgaaagac aataaaaga ctggaggcc 
agtgatctc gaaggcatg ttcatggaa agcaattcc 
acctggtgc tactacagg gcgtctcgt tatccgcca 
gatcacccg aaaaaatac aaactaacc aatgcgttt 
tggtacgaa attgggctt cgactcttc tgtgtcata 
gtttttgag catctgatt atgttgact aatggtatt 
ttggcatat tttataccg ccagttccc aaaaaaatc 
aaacgctcg atttattac gaaaatagg aaacatcga 
aatcaagtt ttggacgca atgcacgaa aaggtgaat 
gcgatgcga cggaaggac gtaaaaccg aagaccaaa 
attattaga gaagagtaa attagtttg tgttaatgt 
tgttacttt gtgtttcat aataaaagt tatgggttc 
ag
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