Status
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Gene information
General information
TC015692 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4822335..4825262
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015692
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05874

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Adult morphology
    prothoracic odoriferous glands darker - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015692 RA
      MLNKCRVCL GFSENMKHV THGDPLATM YTSLTKFEW 
      IRDENIASS LCPECETTL EKCHTFLEK CKKSHAILL 
      KEIEEKSEI KEEENDFDD ESESEGKNK SHLEQISVE 
      EVEKAIFEY QERQKGGTY TCIVCNYVL ISLRALSGH 
      MSMKHKDVR ANWCHICNQ TFDNLAEHN HRLLESCPF 
      CEKTIAKKY FVEHLKAHA GIRTIQCCF CTNKFATYS 
      ALQWHYSTK HSDEMKNDI ATTKRKFCQ ICNKRVRSL 
      PRHNSQLHE KTSAPKTHI CHFCTKELK TESGLKTHL 
      KLHKSDGRH TCSYCGKPH RDRATMIQH ERTHTGEKP 
      FICSVCGKG FAQSSGLNT HMKLHTGRP EACHICEKR 
      FCRPSELKL HLRKHAGEK PFLCTECGK AFKQKSHLT 
      EHMNAAHSD VRPFECHYC HKAFKLKSL LTSHLQRHS 
      GYKPFQCES CSYACFTQY RLQQHMKQH NKEVKREIG 
      YVCPVCEKG FSSQAMFNT HVTSAHNAI L
    CDS sequence
    • >TC015692 RA
      atgttaaac aaatgtagg gtgtgttta ggttttagc 
      gagaacatg aaacatgta acacacgga gatcccttg 
      gcgacaatg tacacatct ctaacgaag tttgagtgg 
      attagggat gaaaacatc gcgtctagt ttgtgcccc 
      gaatgcgaa accactctg gaaaagtgt cacactttt 
      ttggaaaaa tgcaaaaaa agccatgcg attttattg 
      aaagaaatt gaagaaaaa agtgaaatt aaagaagaa 
      gagaacgat tttgatgat gagagtgaa agtgagggc 
      aaaaacaaa tcacacctc gaacaaatt tcggtggaa 
      gaggtcgaa aaagcgatt ttcgagtac caggagaga 
      cagaaaggg ggcacttat acgtgcatt gtttgcaac 
      tatgtctta atctcgctt agggctctt tcaggccac 
      atgtcaatg aaacacaaa gatgtgcgc gctaattgg 
      tgtcatatc tgtaaccaa acatttgac aatttggcg 
      gaacataac catagacta cttgaatcg tgtccattt 
      tgtgagaaa acaattgca aaaaaatat ttcgtagag 
      cacttgaaa gcacacgct gggattaga acgattcag 
      tgttgtttt tgtaccaat aaatttgcc acatattca 
      gccttgcag tggcattat agcacaaaa cactcggat 
      gaaatgaaa aatgacata gccacaact aagcgaaaa 
      ttttgccaa atatgtaat aaaagagtg agaagtctc 
      ccgagacat aacagccaa ttacacgaa aaaaccagt 
      gcccccaaa acccacatt tgtcatttt tgtacaaaa 
      gaattaaag actgaatca gggctgaaa acccacttg 
      aaactgcac aaatcggac ggccgccat acatgtagc 
      tactgtggg aagccccac agagacagg gctactatg 
      atccaacac gagcggaca catacgggc gaaaaaccg 
      ttcatttgt tccgtgtgc ggaaaaggt tttgcccaa 
      agcagtggc ttaaacacc cacatgaag ttgcataca 
      gggcgtcca gaggcgtgc catatttgc gagaagcga 
      ttttgtagg ccgtcagag cttaagtta catttgcga 
      aaacatgca ggcgaaaag ccgtttttg tgcaccgag 
      tgcggtaaa gcgttcaag cagaagagc catctgacg 
      gaacatatg aatgctgcg catagtgac gtcaggcct 
      tttgaatgt cattactgt cacaaggcg tttaagtta 
      aaatcgttg ctaacttcg catctacaa aggcattct 
      ggttataaa ccgtttcag tgtgagtcg tgctcttat 
      gcctgtttt acacaatac agattgcaa caacacatg 
      aagcaacat aacaaggaa gtaaaaaga gaaatagga 
      tatgtgtgt ccagtgtgt gagaagggc tttagcagc 
      caagccatg tttaatacg catgtgacg tcagcacat 
      aacgccata ttgtaa
    mRNA sequence
    • >TC015692 RA
      caattttac tgtttacgt ttggttgta ctttatcaa 
      ggtcatcag ttatataat ttctatttt tgctttttt 
      tatctttta aatatgtta aacaaatgt agggtgtgt 
      ttaggtttt agcgagaac atgaaacat gtaacacac 
      ggagatccc ttggcgaca atgtacaca tctctaacg 
      aagtttgag tggattagg gatgaaaac atcgcgtct 
      agtttgtgc cccgaatgc gaaaccact ctggaaaag 
      tgtcacact tttttggaa aaatgcaaa aaaagccat 
      gcgatttta ttgaaagaa attgaagaa aaaagtgaa 
      attaaagaa gaagagaac gattttgat gatgagagt 
      gaaagtgag ggcaaaaac aaatcacac ctcgaacaa 
      atttcggtg gaagaggtc gaaaaagcg attttcgag 
      taccaggag agacagaaa gggggcact tatacgtgc 
      attgtttgc aactatgtc ttaatctcg cttagggct 
      ctttcaggc cacatgtca atgaaacac aaagatgtg 
      cgcgctaat tggtgtcat atctgtaac caaacattt 
      gacaatttg gcggaacat aaccataga ctacttgaa 
      tcgtgtcca ttttgtgag aaaacaatt gcaaaaaaa 
      tatttcgta gagcacttg aaagcacac gctgggatt 
      agaacgatt cagtgttgt ttttgtacc aataaattt 
      gccacatat tcagccttg cagtggcat tatagcaca 
      aaacactcg gatgaaatg aaaaatgac atagccaca 
      actaagcga aaattttgc caaatatgt aataaaaga 
      gtgagaagt ctcccgaga cataacagc caattacac 
      gaaaaaacc agtgccccc aaaacccac atttgtcat 
      ttttgtaca aaagaatta aagactgaa tcagggctg 
      aaaacccac ttgaaactg cacaaatcg gacggccgc 
      catacatgt agctactgt gggaagccc cacagagac 
      agggctact atgatccaa cacgagcgg acacatacg 
      ggcgaaaaa ccgttcatt tgttccgtg tgcggaaaa 
      ggttttgcc caaagcagt ggcttaaac acccacatg 
      aagttgcat acagggcgt ccagaggcg tgccatatt 
      tgcgagaag cgattttgt aggccgtca gagcttaag 
      ttacatttg cgaaaacat gcaggcgaa aagccgttt 
      ttgtgcacc gagtgcggt aaagcgttc aagcagaag 
      agccatctg acggaacat atgaatgct gcgcatagt 
      gacgtcagg ccttttgaa tgtcattac tgtcacaag 
      gcgtttaag ttaaaatcg ttgctaact tcgcatcta 
      caaaggcat tctggttat aaaccgttt cagtgtgag 
      tcgtgctct tatgcctgt tttacacaa tacagattg 
      caacaacac atgaagcaa cataacaag gaagtaaaa 
      agagaaata ggatatgtg tgtccagtg tgtgagaag 
      ggctttagc agccaagcc atgtttaat acgcatgtg 
      acgtcagca cataacgcc atattgtaa tataataag 
      atatatttt tgtatttaa acaaataaa ccttgaaaa 
      taata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY