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Gene information
General information
TC015686 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4913595..4928682
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015686
    iBeetle screen iB_05870

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015686 RA
      MAPNNENEF KGRLIAAVK KEVKQVMEE SVTRKFVHE 
ESGSVTSLC AAVEACLSQ GLRRRALGL FKTSSTTAL 
LHKIAKHCK EAAIISKRV QEIENADPT RRSSSSSES 
MNKPTLKKN ASLPSNPLP KYLWIRLAL FEKQLAKII 
DYLVSNANK YYDKDALVA DPDYGSILS SLLVGPCAL 
DYSRTKTPD HFWTDPPAD ELVQRHRIS SGHSTPPSV 
RRPILNFRR SLNASSDDS ITNRTNTQH LAKDYVESL 
HQNSRATLL FGKNNVLVL PVNADVSQP MPGYLSLHQ 
TASGLTIKW TPNQLMNGF TDEVQDKSV YWDYALQVR 
LDEIVYVHC HQDSETGGT VILVGQDGV QRPPIHFPK 
GGHMLAFLS CIETGLLPR GRLDPPLWS QRSGTNSNK 
RRRPLPALS ETEETTKDY VFRIADNST HKDMLKRNE 
LMDTHISLP PKARVQLPS TSTSSESST KSFSIDQNA 
PDSPPSICI QAVCDSMKR QIISRAFYG WLAYCRHLS 
TVRTHLSGL VNSKIVNGE GASDGITVE KWNELCIDG 
VVSDYEEVY RLTYFGGVA HDLRKELWP YLLGHYKFG 
STAQQRLEL SEETKQAYE NTMSEWLAV EAIVRQRDK 
ETQANAIAK LSSESMSGE QVPAQIQRD LSNEVFEDM 
LSDEDSEEK PPQTRRNTI RYESDGEVA EDEVQVNTH 
EELKDFIHN VIVTNASVD MGNLGNDND VSHKNNLDA 
VAEENSSLD ACIEAHGLA SPARSACVS PASSNGGVY 
SQELLETFG LNLHRIEKD VQRCDRNYW YFTVDNLEK 
LRNVMCTYV WEHLDIGYM QGMCDLVAP LLVIFNDES 
LTYACFCHL MERMVENFP NGNAMDCHF ANMRSLIQI 
LDSEMYELM HSHGDYTHF YFCYRWFLL DFKRELVYS 
DVYATWEVI WAAQHVASS HFVLFLALA LLETYRDII 
LSNCMDFTD IIKFFNEMA ERHNASAVL SLARDLVLQ 
LQLLIENK
    CDS sequence
    • >TC015686 RA
      atggctcca aacaatgaa aacgaattc aaagggcgt 
ctcattgcc gccgttaaa aaggaagtc aaacaagtg 
atggaggag tcggtgact cgaaaattc gtccacgaa 
gaaagtggc tctgtgacg tcgctgtgt gcggccgtg 
gaggcgtgt ttgtcccag gggttgaga cgccgcgct 
ttggggctt tttaaaacc tcgtcaacg acggccctc 
ttgcacaaa atcgcaaag cattgcaag gaagcggcg 
attatttcc aaaagggtg caggaaatt gaaaatgcg 
gatccgact agaaggtcg tcgagtagt agcgagagt 
atgaataag ccaactttg aagaaaaat gcttctttg 
ccctcgaac ccgctgccg aagtatttg tggattcgg 
ctggctttg ttcgagaaa cagttggcg aaaattata 
gattatttg gtttcgaat gctaataag tattacgat 
aaggatgcg ctcgtggct gatccagat tatgggagt 
atattaagc tcgctgcta gtggggccc tgtgctctg 
gattattcc aggactaag acaccggac catttctgg 
acggatcca ccagctgac gagttggtt caaagacat 
cgaatttca agtgggcac tcaaccccg ccatcagtc 
cgtcgccca atcctcaat ttccggcgt tccctcaac 
gccagttcc gacgattcg ataacaaat cgaaccaac 
acccagcac ttggctaaa gactacgtc gaaagtctg 
caccaaaac tctcgagcc actttacta ttcggcaaa 
aacaacgtt ctagtattg cctgtaaac gccgatgtt 
tcccaacct atgcccggc tacctcagt ctgcaccaa 
accgcaagc ggtttaacg atcaaatgg acgcctaat 
caattaatg aacggcttc actgacgaa gtccaggat 
aaaagtgtg tactgggat tatgctttg caagtgcgc 
ctcgatgag attgtctac gtgcactgt caccaggac 
agtgaaact ggaggcact gttattcta gtgggccag 
gatggggtg caaagacca ccgattcat ttcccgaaa 
ggggggcac atgttggcc tttctcagt tgtattgag 
acggggttg ttgccaagg gggcgtctg gatccgccc 
ttgtggtcg cagaggtcg gggactaat tccaataaa 
cgccgaagg cctcttccg gctctttcc gagacggag 
gagaccacc aaagattac gtttttcgg attgctgat 
aacagcacc cataaagac atgcttaaa cgaaacgaa 
ctaatggac acgcacatt tccttgccg cctaaagcc 
cgcgtccaa ctccccagc acaagtaca agttccgaa 
agctcaacc aagagcttt agtatagac caaaacgcg 
cccgacagt ccgccaagt atttgtatt caagcagtc 
tgtgattcc atgaaacgc caaatcatc tctagggct 
ttctacggc tggctggct tactgccgc catttatcc 
accgtcagg acccacctt agcggatta gttaattcg 
aaaatcgtc aacggagag ggtgccagt gacggcatc 
accgtagag aaatggaac gaactgtgc atcgacggt 
gtagtgagt gactatgag gaagtttac cgtctcact 
tacttcggg ggcgtagcc cacgacctc cgcaaagag 
ctctggccc tacctcctg ggccactac aaattcggc 
agtacagcc cagcaacga ctagaacta agcgaagag 
accaaacaa gcgtacgaa aacaccatg tccgagtgg 
ctggcagtg gaggcaatc gtgcgacaa cgggataaa 
gaaacgcaa gccaatgct atcgcaaaa ctgagcagt 
gaaagtatg agtggagag caagtgcca gctcagatc 
cagcgcgac ctaagcaat gaagttttc gaagatatg 
ttaagtgat gaggattcg gaggaaaaa ccgccacaa 
acacgacgg aacactatt aggtatgag agcgatggg 
gaagtggca gaagatgaa gtacaagtt aatacacat 
gaggagttg aaagatttt atacataat gttattgtg 
actaacgct agcgtggat atggggaat ttgggtaat 
gataacgat gtaagtcat aaaaataac ttggatgcg 
gttgctgaa gaaaattcg tcacttgat gcctgtatt 
gaagcgcat gggttggca tcaccggct aggtcggcc 
tgtgtttcc ccggctagc tccaatgga ggggtgtac 
agtcaagaa ctgctcgag actttcggc ctgaacctg 
caccgaatc gaaaaagac gtgcagcga tgcgaccgc 
aactattgg tatttcaca gtcgacaat ctcgaaaaa 
ctacgcaac gtcatgtgc acgtacgtc tgggaacac 
ttggacatt ggctacatg caaggaatg tgtgacttg 
gtcgcccct ttactagta attttcaac gacgaatca 
ttaacttac gcctgcttt tgccatttg atggaacga 
atggttgaa aatttccca aacggcaat gccatggac 
tgccacttt gccaacatg cgctctctg atccaaatc 
ctcgactca gaaatgtac gagctgatg cactcacac 
ggcgattac acacacttt tatttctgc taccgctgg 
tttttgctc gactttaaa agagagctg gtttattcg 
gatgtttac gcgacttgg gaggtgatt tgggccgca 
caacacgtt gcctcaagt cattttgtc ctgtttttg 
gccttggcc cttttggaa acgtaccga gatattatc 
ctgtccaac tgtatggac ttcacagac ataatcaag 
ttttttaac gaaatggct gaacgacac aacgcttca 
gccgttctg tctctggcc agagatttg gtgctccaa 
ctgcaactg ttgattgaa aacaagtag
    mRNA sequence
    • >TC015686 RA
      caaaagtgg caatttccg catcgattt tccttcaag 
gtagtcaaa aaatttgat gggttcgca cggacgtgg 
cgcatttag ccacaataa aaccctaat cggttatcg 
cacaatttc ctttcggtt taatcagtg agctgcaat 
cagtgaaat ggctccaaa caatgaaaa cgaattcaa 
agggcgtct cattgccgc cgttaaaaa ggaagtcaa 
acaagtgat ggaggagtc ggtgactcg aaaattcgt 
ccacgaaga aagtggctc tgtgacgtc gctgtgtgc 
ggccgtgga ggcgtgttt gtcccaggg gttgagacg 
ccgcgcttt ggggctttt taaaacctc gtcaacgac 
ggccctctt gcacaaaat cgcaaagca ttgcaagga 
agcggcgat tatttccaa aagggtgca ggaaattga 
aaatgcgga tccgactag aaggtcgtc gagtagtag 
cgagagtat gaataagcc aactttgaa gaaaaatgc 
ttctttgcc ctcgaaccc gctgccgaa gtatttgtg 
gattcggct ggctttgtt cgagaaaca gttggcgaa 
aattataga ttatttggt ttcgaatgc taataagta 
ttacgataa ggatgcgct cgtggctga tccagatta 
tgggagtat attaagctc gctgctagt ggggccctg 
tgctctgga ttattccag gactaagac accggacca 
tttctggac ggatccacc agctgacga gttggttca 
aagacatcg aatttcaag tgggcactc aaccccgcc 
atcagtccg tcgcccaat cctcaattt ccggcgttc 
cctcaacgc cagttccga cgattcgat aacaaatcg 
aaccaacac ccagcactt ggctaaaga ctacgtcga 
aagtctgca ccaaaactc tcgagccac tttactatt 
cggcaaaaa caacgttct agtattgcc tgtaaacgc 
cgatgtttc ccaacctat gcccggcta cctcagtct 
gcaccaaac cgcaagcgg tttaacgat caaatggac 
gcctaatca attaatgaa cggcttcac tgacgaagt 
ccaggataa aagtgtgta ctgggatta tgctttgca 
agtgcgcct cgatgagat tgtctacgt gcactgtca 
ccaggacag tgaaactgg aggcactgt tattctagt 
gggccagga tggggtgca aagaccacc gattcattt 
cccgaaagg ggggcacat gttggcctt tctcagttg 
tattgagac ggggttgtt gccaagggg gcgtctgga 
tccgccctt gtggtcgca gaggtcggg gactaattc 
caataaacg ccgaaggcc tcttccggc tctttccga 
gacggagga gaccaccaa agattacgt ttttcggat 
tgctgataa cagcaccca taaagacat gcttaaacg 
aaacgaact aatggacac gcacatttc cttgccgcc 
taaagcccg cgtccaact ccccagcac aagtacaag 
ttccgaaag ctcaaccaa gagctttag tatagacca 
aaacgcgcc cgacagtcc gccaagtat ttgtattca 
agcagtctg tgattccat gaaacgcca aatcatctc 
tagggcttt ctacggctg gctggctta ctgccgcca 
tttatccac cgtcaggac ccaccttag cggattagt 
taattcgaa aatcgtcaa cggagaggg tgccagtga 
cggcatcac cgtagagaa atggaacga actgtgcat 
cgacggtgt agtgagtga ctatgagga agtttaccg 
tctcactta cttcggggg cgtagccca cgacctccg 
caaagagct ctggcccta cctcctggg ccactacaa 
attcggcag tacagccca gcaacgact agaactaag 
cgaagagac caaacaagc gtacgaaaa caccatgtc 
cgagtggct ggcagtgga ggcaatcgt gcgacaacg 
ggataaaga aacgcaagc caatgctat cgcaaaact 
gagcagtga aagtatgag tggagagca agtgccagc 
tcagatcca gcgcgacct aagcaatga agttttcga 
agatatgtt aagtgatga ggattcgga ggaaaaacc 
gccacaaac acgacggaa cactattag gtatgagag 
cgatgggga agtggcaga agatgaagt acaagttaa 
tacacatga ggagttgaa agattttat acataatgt 
tattgtgac taacgctag cgtggatat ggggaattt 
gggtaatga taacgatgt aagtcataa aaataactt 
ggatgcggt tgctgaaga aaattcgtc acttgatgc 
ctgtattga agcgcatgg gttggcatc accggctag 
gtcggcctg tgtttcccc ggctagctc caatggagg 
ggtgtacag tcaagaact gctcgagac tttcggcct 
gaacctgca ccgaatcga aaaagacgt gcagcgatg 
cgaccgcaa ctattggta tttcacagt cgacaatct 
cgaaaaact acgcaacgt catgtgcac gtacgtctg 
ggaacactt ggacattgg ctacatgca aggaatgtg 
tgacttggt cgccccttt actagtaat tttcaacga 
cgaatcatt aacttacgc ctgcttttg ccatttgat 
ggaacgaat ggttgaaaa tttcccaaa cggcaatgc 
catggactg ccactttgc caacatgcg ctctctgat 
ccaaatcct cgactcaga aatgtacga gctgatgca 
ctcacacgg cgattacac acactttta tttctgcta 
ccgctggtt tttgctcga ctttaaaag agagctggt 
ttattcgga tgtttacgc gacttggga ggtgatttg 
ggccgcaca acacgttgc ctcaagtca ttttgtcct 
gtttttggc cttggccct tttggaaac gtaccgaga 
tattatcct gtccaactg tatggactt cacagacat 
aatcaagtt ttttaacga aatggctga acgacacaa 
cgcttcagc cgttctgtc tctggccag agatttggt 
gctccaact gcaactgtt gattgaaaa caagtagaa 
ctcgatgta gtgtctcta ttttacgta ctagatgga 
ggagaaatc aaaaatacc tcactattt ttaaaatgt 
taggtctcg ggcgaggtg attgtttca ttaacccaa 
caaagtgtg ccaaatgtt tcagttttt ggaaataat 
tggttaaat gtgcttcat atttttata ttattgttg 
ttgcattgg cgttttttt cgcaacgtt tggattcta 
aagtgttaa cggtttgta agatacgat atttatttt 
gttaaaaaa gatagtttg tgtaaaata ttaatatac 
atatgaata tatatttta taaaaagtt agggaaggt 
ggaattcaa ttctatatt ctgtagttt atacactta 
acaacaaac aactattac caaatacaa atattacaa 
cgtcttaac ttaccacta ttttcatgt atgaatgtt 
taaatgtgc cttttttaa gcatctttc acacagtat 
tttatccgt atcaagacg taacaaagt tcaagcggg 
actttattg caaacagtg ccaaagcag gcccgctta 
gctttctta tctgatgat ttaacgaaa tgtaaatag 
aacatatca atcttctta tctcatgtt gtaccaaat 
atagtaatt atttaatat actactgtg tgtttcact 
cggaatcgc tattggcgg ccaggactt cccctaaca 
ccgtttcgt gaaacgtcg ggtcgtagc aacgaccga 
atactgttt tcgctttct tacttcggg gagattcgg 
gtgattttg gtatgacgt
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