| General information | |||
| TC015686 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 4913595..4928682 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015686 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05870 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015686 RA
MAPNNENEF KGRLIAAVK KEVKQVMEE SVTRKFVHE ESGSVTSLC AAVEACLSQ GLRRRALGL FKTSSTTAL LHKIAKHCK EAAIISKRV QEIENADPT RRSSSSSES MNKPTLKKN ASLPSNPLP KYLWIRLAL FEKQLAKII DYLVSNANK YYDKDALVA DPDYGSILS SLLVGPCAL DYSRTKTPD HFWTDPPAD ELVQRHRIS SGHSTPPSV RRPILNFRR SLNASSDDS ITNRTNTQH LAKDYVESL HQNSRATLL FGKNNVLVL PVNADVSQP MPGYLSLHQ TASGLTIKW TPNQLMNGF TDEVQDKSV YWDYALQVR LDEIVYVHC HQDSETGGT VILVGQDGV QRPPIHFPK GGHMLAFLS CIETGLLPR GRLDPPLWS QRSGTNSNK RRRPLPALS ETEETTKDY VFRIADNST HKDMLKRNE LMDTHISLP PKARVQLPS TSTSSESST KSFSIDQNA PDSPPSICI QAVCDSMKR QIISRAFYG WLAYCRHLS TVRTHLSGL VNSKIVNGE GASDGITVE KWNELCIDG VVSDYEEVY RLTYFGGVA HDLRKELWP YLLGHYKFG STAQQRLEL SEETKQAYE NTMSEWLAV EAIVRQRDK ETQANAIAK LSSESMSGE QVPAQIQRD LSNEVFEDM LSDEDSEEK PPQTRRNTI RYESDGEVA EDEVQVNTH EELKDFIHN VIVTNASVD MGNLGNDND VSHKNNLDA VAEENSSLD ACIEAHGLA SPARSACVS PASSNGGVY SQELLETFG LNLHRIEKD VQRCDRNYW YFTVDNLEK LRNVMCTYV WEHLDIGYM QGMCDLVAP LLVIFNDES LTYACFCHL MERMVENFP NGNAMDCHF ANMRSLIQI LDSEMYELM HSHGDYTHF YFCYRWFLL DFKRELVYS DVYATWEVI WAAQHVASS HFVLFLALA LLETYRDII LSNCMDFTD IIKFFNEMA ERHNASAVL SLARDLVLQ LQLLIENK
- >TC015686 RA
atggctcca aacaatgaa aacgaattc aaagggcgt ctcattgcc gccgttaaa aaggaagtc aaacaagtg atggaggag tcggtgact cgaaaattc gtccacgaa gaaagtggc tctgtgacg tcgctgtgt gcggccgtg gaggcgtgt ttgtcccag gggttgaga cgccgcgct ttggggctt tttaaaacc tcgtcaacg acggccctc ttgcacaaa atcgcaaag cattgcaag gaagcggcg attatttcc aaaagggtg caggaaatt gaaaatgcg gatccgact agaaggtcg tcgagtagt agcgagagt atgaataag ccaactttg aagaaaaat gcttctttg ccctcgaac ccgctgccg aagtatttg tggattcgg ctggctttg ttcgagaaa cagttggcg aaaattata gattatttg gtttcgaat gctaataag tattacgat aaggatgcg ctcgtggct gatccagat tatgggagt atattaagc tcgctgcta gtggggccc tgtgctctg gattattcc aggactaag acaccggac catttctgg acggatcca ccagctgac gagttggtt caaagacat cgaatttca agtgggcac tcaaccccg ccatcagtc cgtcgccca atcctcaat ttccggcgt tccctcaac gccagttcc gacgattcg ataacaaat cgaaccaac acccagcac ttggctaaa gactacgtc gaaagtctg caccaaaac tctcgagcc actttacta ttcggcaaa aacaacgtt ctagtattg cctgtaaac gccgatgtt tcccaacct atgcccggc tacctcagt ctgcaccaa accgcaagc ggtttaacg atcaaatgg acgcctaat caattaatg aacggcttc actgacgaa gtccaggat aaaagtgtg tactgggat tatgctttg caagtgcgc ctcgatgag attgtctac gtgcactgt caccaggac agtgaaact ggaggcact gttattcta gtgggccag gatggggtg caaagacca ccgattcat ttcccgaaa ggggggcac atgttggcc tttctcagt tgtattgag acggggttg ttgccaagg gggcgtctg gatccgccc ttgtggtcg cagaggtcg gggactaat tccaataaa cgccgaagg cctcttccg gctctttcc gagacggag gagaccacc aaagattac gtttttcgg attgctgat aacagcacc cataaagac atgcttaaa cgaaacgaa ctaatggac acgcacatt tccttgccg cctaaagcc cgcgtccaa ctccccagc acaagtaca agttccgaa agctcaacc aagagcttt agtatagac caaaacgcg cccgacagt ccgccaagt atttgtatt caagcagtc tgtgattcc atgaaacgc caaatcatc tctagggct ttctacggc tggctggct tactgccgc catttatcc accgtcagg acccacctt agcggatta gttaattcg aaaatcgtc aacggagag ggtgccagt gacggcatc accgtagag aaatggaac gaactgtgc atcgacggt gtagtgagt gactatgag gaagtttac cgtctcact tacttcggg ggcgtagcc cacgacctc cgcaaagag ctctggccc tacctcctg ggccactac aaattcggc agtacagcc cagcaacga ctagaacta agcgaagag accaaacaa gcgtacgaa aacaccatg tccgagtgg ctggcagtg gaggcaatc gtgcgacaa cgggataaa gaaacgcaa gccaatgct atcgcaaaa ctgagcagt gaaagtatg agtggagag caagtgcca gctcagatc cagcgcgac ctaagcaat gaagttttc gaagatatg ttaagtgat gaggattcg gaggaaaaa ccgccacaa acacgacgg aacactatt aggtatgag agcgatggg gaagtggca gaagatgaa gtacaagtt aatacacat gaggagttg aaagatttt atacataat gttattgtg actaacgct agcgtggat atggggaat ttgggtaat gataacgat gtaagtcat aaaaataac ttggatgcg gttgctgaa gaaaattcg tcacttgat gcctgtatt gaagcgcat gggttggca tcaccggct aggtcggcc tgtgtttcc ccggctagc tccaatgga ggggtgtac agtcaagaa ctgctcgag actttcggc ctgaacctg caccgaatc gaaaaagac gtgcagcga tgcgaccgc aactattgg tatttcaca gtcgacaat ctcgaaaaa ctacgcaac gtcatgtgc acgtacgtc tgggaacac ttggacatt ggctacatg caaggaatg tgtgacttg gtcgcccct ttactagta attttcaac gacgaatca ttaacttac gcctgcttt tgccatttg atggaacga atggttgaa aatttccca aacggcaat gccatggac tgccacttt gccaacatg cgctctctg atccaaatc ctcgactca gaaatgtac gagctgatg cactcacac ggcgattac acacacttt tatttctgc taccgctgg tttttgctc gactttaaa agagagctg gtttattcg gatgtttac gcgacttgg gaggtgatt tgggccgca caacacgtt gcctcaagt cattttgtc ctgtttttg gccttggcc cttttggaa acgtaccga gatattatc ctgtccaac tgtatggac ttcacagac ataatcaag ttttttaac gaaatggct gaacgacac aacgcttca gccgttctg tctctggcc agagatttg gtgctccaa ctgcaactg ttgattgaa aacaagtag
- >TC015686 RA
caaaagtgg caatttccg catcgattt tccttcaag gtagtcaaa aaatttgat gggttcgca cggacgtgg cgcatttag ccacaataa aaccctaat cggttatcg cacaatttc ctttcggtt taatcagtg agctgcaat cagtgaaat ggctccaaa caatgaaaa cgaattcaa agggcgtct cattgccgc cgttaaaaa ggaagtcaa acaagtgat ggaggagtc ggtgactcg aaaattcgt ccacgaaga aagtggctc tgtgacgtc gctgtgtgc ggccgtgga ggcgtgttt gtcccaggg gttgagacg ccgcgcttt ggggctttt taaaacctc gtcaacgac ggccctctt gcacaaaat cgcaaagca ttgcaagga agcggcgat tatttccaa aagggtgca ggaaattga aaatgcgga tccgactag aaggtcgtc gagtagtag cgagagtat gaataagcc aactttgaa gaaaaatgc ttctttgcc ctcgaaccc gctgccgaa gtatttgtg gattcggct ggctttgtt cgagaaaca gttggcgaa aattataga ttatttggt ttcgaatgc taataagta ttacgataa ggatgcgct cgtggctga tccagatta tgggagtat attaagctc gctgctagt ggggccctg tgctctgga ttattccag gactaagac accggacca tttctggac ggatccacc agctgacga gttggttca aagacatcg aatttcaag tgggcactc aaccccgcc atcagtccg tcgcccaat cctcaattt ccggcgttc cctcaacgc cagttccga cgattcgat aacaaatcg aaccaacac ccagcactt ggctaaaga ctacgtcga aagtctgca ccaaaactc tcgagccac tttactatt cggcaaaaa caacgttct agtattgcc tgtaaacgc cgatgtttc ccaacctat gcccggcta cctcagtct gcaccaaac cgcaagcgg tttaacgat caaatggac gcctaatca attaatgaa cggcttcac tgacgaagt ccaggataa aagtgtgta ctgggatta tgctttgca agtgcgcct cgatgagat tgtctacgt gcactgtca ccaggacag tgaaactgg aggcactgt tattctagt gggccagga tggggtgca aagaccacc gattcattt cccgaaagg ggggcacat gttggcctt tctcagttg tattgagac ggggttgtt gccaagggg gcgtctgga tccgccctt gtggtcgca gaggtcggg gactaattc caataaacg ccgaaggcc tcttccggc tctttccga gacggagga gaccaccaa agattacgt ttttcggat tgctgataa cagcaccca taaagacat gcttaaacg aaacgaact aatggacac gcacatttc cttgccgcc taaagcccg cgtccaact ccccagcac aagtacaag ttccgaaag ctcaaccaa gagctttag tatagacca aaacgcgcc cgacagtcc gccaagtat ttgtattca agcagtctg tgattccat gaaacgcca aatcatctc tagggcttt ctacggctg gctggctta ctgccgcca tttatccac cgtcaggac ccaccttag cggattagt taattcgaa aatcgtcaa cggagaggg tgccagtga cggcatcac cgtagagaa atggaacga actgtgcat cgacggtgt agtgagtga ctatgagga agtttaccg tctcactta cttcggggg cgtagccca cgacctccg caaagagct ctggcccta cctcctggg ccactacaa attcggcag tacagccca gcaacgact agaactaag cgaagagac caaacaagc gtacgaaaa caccatgtc cgagtggct ggcagtgga ggcaatcgt gcgacaacg ggataaaga aacgcaagc caatgctat cgcaaaact gagcagtga aagtatgag tggagagca agtgccagc tcagatcca gcgcgacct aagcaatga agttttcga agatatgtt aagtgatga ggattcgga ggaaaaacc gccacaaac acgacggaa cactattag gtatgagag cgatgggga agtggcaga agatgaagt acaagttaa tacacatga ggagttgaa agattttat acataatgt tattgtgac taacgctag cgtggatat ggggaattt gggtaatga taacgatgt aagtcataa aaataactt ggatgcggt tgctgaaga aaattcgtc acttgatgc ctgtattga agcgcatgg gttggcatc accggctag gtcggcctg tgtttcccc ggctagctc caatggagg ggtgtacag tcaagaact gctcgagac tttcggcct gaacctgca ccgaatcga aaaagacgt gcagcgatg cgaccgcaa ctattggta tttcacagt cgacaatct cgaaaaact acgcaacgt catgtgcac gtacgtctg ggaacactt ggacattgg ctacatgca aggaatgtg tgacttggt cgccccttt actagtaat tttcaacga cgaatcatt aacttacgc ctgcttttg ccatttgat ggaacgaat ggttgaaaa tttcccaaa cggcaatgc catggactg ccactttgc caacatgcg ctctctgat ccaaatcct cgactcaga aatgtacga gctgatgca ctcacacgg cgattacac acactttta tttctgcta ccgctggtt tttgctcga ctttaaaag agagctggt ttattcgga tgtttacgc gacttggga ggtgatttg ggccgcaca acacgttgc ctcaagtca ttttgtcct gtttttggc cttggccct tttggaaac gtaccgaga tattatcct gtccaactg tatggactt cacagacat aatcaagtt ttttaacga aatggctga acgacacaa cgcttcagc cgttctgtc tctggccag agatttggt gctccaact gcaactgtt gattgaaaa caagtagaa ctcgatgta gtgtctcta ttttacgta ctagatgga ggagaaatc aaaaatacc tcactattt ttaaaatgt taggtctcg ggcgaggtg attgtttca ttaacccaa caaagtgtg ccaaatgtt tcagttttt ggaaataat tggttaaat gtgcttcat atttttata ttattgttg ttgcattgg cgttttttt cgcaacgtt tggattcta aagtgttaa cggtttgta agatacgat atttatttt gttaaaaaa gatagtttg tgtaaaata ttaatatac atatgaata tatatttta taaaaagtt agggaaggt ggaattcaa ttctatatt ctgtagttt atacactta acaacaaac aactattac caaatacaa atattacaa cgtcttaac ttaccacta ttttcatgt atgaatgtt taaatgtgc cttttttaa gcatctttc acacagtat tttatccgt atcaagacg taacaaagt tcaagcggg actttattg caaacagtg ccaaagcag gcccgctta gctttctta tctgatgat ttaacgaaa tgtaaatag aacatatca atcttctta tctcatgtt gtaccaaat atagtaatt atttaatat actactgtg tgtttcact cggaatcgc tattggcgg ccaggactt cccctaaca ccgtttcgt gaaacgtcg ggtcgtagc aacgaccga atactgttt tcgctttct tacttcggg gagattcgg gtgattttg gtatgacgt
