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Gene information
General information
TC015686 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4913595..4928682
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015686
    iBeetle screen iB_05870

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015686 RA
      MAPNNENEF KGRLIAAVK KEVKQVMEE SVTRKFVHE 
      ESGSVTSLC AAVEACLSQ GLRRRALGL FKTSSTTAL 
      LHKIAKHCK EAAIISKRV QEIENADPT RRSSSSSES 
      MNKPTLKKN ASLPSNPLP KYLWIRLAL FEKQLAKII 
      DYLVSNANK YYDKDALVA DPDYGSILS SLLVGPCAL 
      DYSRTKTPD HFWTDPPAD ELVQRHRIS SGHSTPPSV 
      RRPILNFRR SLNASSDDS ITNRTNTQH LAKDYVESL 
      HQNSRATLL FGKNNVLVL PVNADVSQP MPGYLSLHQ 
      TASGLTIKW TPNQLMNGF TDEVQDKSV YWDYALQVR 
      LDEIVYVHC HQDSETGGT VILVGQDGV QRPPIHFPK 
      GGHMLAFLS CIETGLLPR GRLDPPLWS QRSGTNSNK 
      RRRPLPALS ETEETTKDY VFRIADNST HKDMLKRNE 
      LMDTHISLP PKARVQLPS TSTSSESST KSFSIDQNA 
      PDSPPSICI QAVCDSMKR QIISRAFYG WLAYCRHLS 
      TVRTHLSGL VNSKIVNGE GASDGITVE KWNELCIDG 
      VVSDYEEVY RLTYFGGVA HDLRKELWP YLLGHYKFG 
      STAQQRLEL SEETKQAYE NTMSEWLAV EAIVRQRDK 
      ETQANAIAK LSSESMSGE QVPAQIQRD LSNEVFEDM 
      LSDEDSEEK PPQTRRNTI RYESDGEVA EDEVQVNTH 
      EELKDFIHN VIVTNASVD MGNLGNDND VSHKNNLDA 
      VAEENSSLD ACIEAHGLA SPARSACVS PASSNGGVY 
      SQELLETFG LNLHRIEKD VQRCDRNYW YFTVDNLEK 
      LRNVMCTYV WEHLDIGYM QGMCDLVAP LLVIFNDES 
      LTYACFCHL MERMVENFP NGNAMDCHF ANMRSLIQI 
      LDSEMYELM HSHGDYTHF YFCYRWFLL DFKRELVYS 
      DVYATWEVI WAAQHVASS HFVLFLALA LLETYRDII 
      LSNCMDFTD IIKFFNEMA ERHNASAVL SLARDLVLQ 
      LQLLIENK
    CDS sequence
    • >TC015686 RA
      atggctcca aacaatgaa aacgaattc aaagggcgt 
      ctcattgcc gccgttaaa aaggaagtc aaacaagtg 
      atggaggag tcggtgact cgaaaattc gtccacgaa 
      gaaagtggc tctgtgacg tcgctgtgt gcggccgtg 
      gaggcgtgt ttgtcccag gggttgaga cgccgcgct 
      ttggggctt tttaaaacc tcgtcaacg acggccctc 
      ttgcacaaa atcgcaaag cattgcaag gaagcggcg 
      attatttcc aaaagggtg caggaaatt gaaaatgcg 
      gatccgact agaaggtcg tcgagtagt agcgagagt 
      atgaataag ccaactttg aagaaaaat gcttctttg 
      ccctcgaac ccgctgccg aagtatttg tggattcgg 
      ctggctttg ttcgagaaa cagttggcg aaaattata 
      gattatttg gtttcgaat gctaataag tattacgat 
      aaggatgcg ctcgtggct gatccagat tatgggagt 
      atattaagc tcgctgcta gtggggccc tgtgctctg 
      gattattcc aggactaag acaccggac catttctgg 
      acggatcca ccagctgac gagttggtt caaagacat 
      cgaatttca agtgggcac tcaaccccg ccatcagtc 
      cgtcgccca atcctcaat ttccggcgt tccctcaac 
      gccagttcc gacgattcg ataacaaat cgaaccaac 
      acccagcac ttggctaaa gactacgtc gaaagtctg 
      caccaaaac tctcgagcc actttacta ttcggcaaa 
      aacaacgtt ctagtattg cctgtaaac gccgatgtt 
      tcccaacct atgcccggc tacctcagt ctgcaccaa 
      accgcaagc ggtttaacg atcaaatgg acgcctaat 
      caattaatg aacggcttc actgacgaa gtccaggat 
      aaaagtgtg tactgggat tatgctttg caagtgcgc 
      ctcgatgag attgtctac gtgcactgt caccaggac 
      agtgaaact ggaggcact gttattcta gtgggccag 
      gatggggtg caaagacca ccgattcat ttcccgaaa 
      ggggggcac atgttggcc tttctcagt tgtattgag 
      acggggttg ttgccaagg gggcgtctg gatccgccc 
      ttgtggtcg cagaggtcg gggactaat tccaataaa 
      cgccgaagg cctcttccg gctctttcc gagacggag 
      gagaccacc aaagattac gtttttcgg attgctgat 
      aacagcacc cataaagac atgcttaaa cgaaacgaa 
      ctaatggac acgcacatt tccttgccg cctaaagcc 
      cgcgtccaa ctccccagc acaagtaca agttccgaa 
      agctcaacc aagagcttt agtatagac caaaacgcg 
      cccgacagt ccgccaagt atttgtatt caagcagtc 
      tgtgattcc atgaaacgc caaatcatc tctagggct 
      ttctacggc tggctggct tactgccgc catttatcc 
      accgtcagg acccacctt agcggatta gttaattcg 
      aaaatcgtc aacggagag ggtgccagt gacggcatc 
      accgtagag aaatggaac gaactgtgc atcgacggt 
      gtagtgagt gactatgag gaagtttac cgtctcact 
      tacttcggg ggcgtagcc cacgacctc cgcaaagag 
      ctctggccc tacctcctg ggccactac aaattcggc 
      agtacagcc cagcaacga ctagaacta agcgaagag 
      accaaacaa gcgtacgaa aacaccatg tccgagtgg 
      ctggcagtg gaggcaatc gtgcgacaa cgggataaa 
      gaaacgcaa gccaatgct atcgcaaaa ctgagcagt 
      gaaagtatg agtggagag caagtgcca gctcagatc 
      cagcgcgac ctaagcaat gaagttttc gaagatatg 
      ttaagtgat gaggattcg gaggaaaaa ccgccacaa 
      acacgacgg aacactatt aggtatgag agcgatggg 
      gaagtggca gaagatgaa gtacaagtt aatacacat 
      gaggagttg aaagatttt atacataat gttattgtg 
      actaacgct agcgtggat atggggaat ttgggtaat 
      gataacgat gtaagtcat aaaaataac ttggatgcg 
      gttgctgaa gaaaattcg tcacttgat gcctgtatt 
      gaagcgcat gggttggca tcaccggct aggtcggcc 
      tgtgtttcc ccggctagc tccaatgga ggggtgtac 
      agtcaagaa ctgctcgag actttcggc ctgaacctg 
      caccgaatc gaaaaagac gtgcagcga tgcgaccgc 
      aactattgg tatttcaca gtcgacaat ctcgaaaaa 
      ctacgcaac gtcatgtgc acgtacgtc tgggaacac 
      ttggacatt ggctacatg caaggaatg tgtgacttg 
      gtcgcccct ttactagta attttcaac gacgaatca 
      ttaacttac gcctgcttt tgccatttg atggaacga 
      atggttgaa aatttccca aacggcaat gccatggac 
      tgccacttt gccaacatg cgctctctg atccaaatc 
      ctcgactca gaaatgtac gagctgatg cactcacac 
      ggcgattac acacacttt tatttctgc taccgctgg 
      tttttgctc gactttaaa agagagctg gtttattcg 
      gatgtttac gcgacttgg gaggtgatt tgggccgca 
      caacacgtt gcctcaagt cattttgtc ctgtttttg 
      gccttggcc cttttggaa acgtaccga gatattatc 
      ctgtccaac tgtatggac ttcacagac ataatcaag 
      ttttttaac gaaatggct gaacgacac aacgcttca 
      gccgttctg tctctggcc agagatttg gtgctccaa 
      ctgcaactg ttgattgaa aacaagtag
    mRNA sequence
    • >TC015686 RA
      caaaagtgg caatttccg catcgattt tccttcaag 
      gtagtcaaa aaatttgat gggttcgca cggacgtgg 
      cgcatttag ccacaataa aaccctaat cggttatcg 
      cacaatttc ctttcggtt taatcagtg agctgcaat 
      cagtgaaat ggctccaaa caatgaaaa cgaattcaa 
      agggcgtct cattgccgc cgttaaaaa ggaagtcaa 
      acaagtgat ggaggagtc ggtgactcg aaaattcgt 
      ccacgaaga aagtggctc tgtgacgtc gctgtgtgc 
      ggccgtgga ggcgtgttt gtcccaggg gttgagacg 
      ccgcgcttt ggggctttt taaaacctc gtcaacgac 
      ggccctctt gcacaaaat cgcaaagca ttgcaagga 
      agcggcgat tatttccaa aagggtgca ggaaattga 
      aaatgcgga tccgactag aaggtcgtc gagtagtag 
      cgagagtat gaataagcc aactttgaa gaaaaatgc 
      ttctttgcc ctcgaaccc gctgccgaa gtatttgtg 
      gattcggct ggctttgtt cgagaaaca gttggcgaa 
      aattataga ttatttggt ttcgaatgc taataagta 
      ttacgataa ggatgcgct cgtggctga tccagatta 
      tgggagtat attaagctc gctgctagt ggggccctg 
      tgctctgga ttattccag gactaagac accggacca 
      tttctggac ggatccacc agctgacga gttggttca 
      aagacatcg aatttcaag tgggcactc aaccccgcc 
      atcagtccg tcgcccaat cctcaattt ccggcgttc 
      cctcaacgc cagttccga cgattcgat aacaaatcg 
      aaccaacac ccagcactt ggctaaaga ctacgtcga 
      aagtctgca ccaaaactc tcgagccac tttactatt 
      cggcaaaaa caacgttct agtattgcc tgtaaacgc 
      cgatgtttc ccaacctat gcccggcta cctcagtct 
      gcaccaaac cgcaagcgg tttaacgat caaatggac 
      gcctaatca attaatgaa cggcttcac tgacgaagt 
      ccaggataa aagtgtgta ctgggatta tgctttgca 
      agtgcgcct cgatgagat tgtctacgt gcactgtca 
      ccaggacag tgaaactgg aggcactgt tattctagt 
      gggccagga tggggtgca aagaccacc gattcattt 
      cccgaaagg ggggcacat gttggcctt tctcagttg 
      tattgagac ggggttgtt gccaagggg gcgtctgga 
      tccgccctt gtggtcgca gaggtcggg gactaattc 
      caataaacg ccgaaggcc tcttccggc tctttccga 
      gacggagga gaccaccaa agattacgt ttttcggat 
      tgctgataa cagcaccca taaagacat gcttaaacg 
      aaacgaact aatggacac gcacatttc cttgccgcc 
      taaagcccg cgtccaact ccccagcac aagtacaag 
      ttccgaaag ctcaaccaa gagctttag tatagacca 
      aaacgcgcc cgacagtcc gccaagtat ttgtattca 
      agcagtctg tgattccat gaaacgcca aatcatctc 
      tagggcttt ctacggctg gctggctta ctgccgcca 
      tttatccac cgtcaggac ccaccttag cggattagt 
      taattcgaa aatcgtcaa cggagaggg tgccagtga 
      cggcatcac cgtagagaa atggaacga actgtgcat 
      cgacggtgt agtgagtga ctatgagga agtttaccg 
      tctcactta cttcggggg cgtagccca cgacctccg 
      caaagagct ctggcccta cctcctggg ccactacaa 
      attcggcag tacagccca gcaacgact agaactaag 
      cgaagagac caaacaagc gtacgaaaa caccatgtc 
      cgagtggct ggcagtgga ggcaatcgt gcgacaacg 
      ggataaaga aacgcaagc caatgctat cgcaaaact 
      gagcagtga aagtatgag tggagagca agtgccagc 
      tcagatcca gcgcgacct aagcaatga agttttcga 
      agatatgtt aagtgatga ggattcgga ggaaaaacc 
      gccacaaac acgacggaa cactattag gtatgagag 
      cgatgggga agtggcaga agatgaagt acaagttaa 
      tacacatga ggagttgaa agattttat acataatgt 
      tattgtgac taacgctag cgtggatat ggggaattt 
      gggtaatga taacgatgt aagtcataa aaataactt 
      ggatgcggt tgctgaaga aaattcgtc acttgatgc 
      ctgtattga agcgcatgg gttggcatc accggctag 
      gtcggcctg tgtttcccc ggctagctc caatggagg 
      ggtgtacag tcaagaact gctcgagac tttcggcct 
      gaacctgca ccgaatcga aaaagacgt gcagcgatg 
      cgaccgcaa ctattggta tttcacagt cgacaatct 
      cgaaaaact acgcaacgt catgtgcac gtacgtctg 
      ggaacactt ggacattgg ctacatgca aggaatgtg 
      tgacttggt cgccccttt actagtaat tttcaacga 
      cgaatcatt aacttacgc ctgcttttg ccatttgat 
      ggaacgaat ggttgaaaa tttcccaaa cggcaatgc 
      catggactg ccactttgc caacatgcg ctctctgat 
      ccaaatcct cgactcaga aatgtacga gctgatgca 
      ctcacacgg cgattacac acactttta tttctgcta 
      ccgctggtt tttgctcga ctttaaaag agagctggt 
      ttattcgga tgtttacgc gacttggga ggtgatttg 
      ggccgcaca acacgttgc ctcaagtca ttttgtcct 
      gtttttggc cttggccct tttggaaac gtaccgaga 
      tattatcct gtccaactg tatggactt cacagacat 
      aatcaagtt ttttaacga aatggctga acgacacaa 
      cgcttcagc cgttctgtc tctggccag agatttggt 
      gctccaact gcaactgtt gattgaaaa caagtagaa 
      ctcgatgta gtgtctcta ttttacgta ctagatgga 
      ggagaaatc aaaaatacc tcactattt ttaaaatgt 
      taggtctcg ggcgaggtg attgtttca ttaacccaa 
      caaagtgtg ccaaatgtt tcagttttt ggaaataat 
      tggttaaat gtgcttcat atttttata ttattgttg 
      ttgcattgg cgttttttt cgcaacgtt tggattcta 
      aagtgttaa cggtttgta agatacgat atttatttt 
      gttaaaaaa gatagtttg tgtaaaata ttaatatac 
      atatgaata tatatttta taaaaagtt agggaaggt 
      ggaattcaa ttctatatt ctgtagttt atacactta 
      acaacaaac aactattac caaatacaa atattacaa 
      cgtcttaac ttaccacta ttttcatgt atgaatgtt 
      taaatgtgc cttttttaa gcatctttc acacagtat 
      tttatccgt atcaagacg taacaaagt tcaagcggg 
      actttattg caaacagtg ccaaagcag gcccgctta 
      gctttctta tctgatgat ttaacgaaa tgtaaatag 
      aacatatca atcttctta tctcatgtt gtaccaaat 
      atagtaatt atttaatat actactgtg tgtttcact 
      cggaatcgc tattggcgg ccaggactt cccctaaca 
      ccgtttcgt gaaacgtcg ggtcgtagc aacgaccga 
      atactgttt tcgctttct tacttcggg gagattcgg 
      gtgattttg gtatgacgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY