Status
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Gene information
General information
TC030908
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015685,TC015684
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4947355..4982407
Sequence information
Transcripts / Proteins (5) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030908
    iBeetle screen iB_05869

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030908 RA
      MLKSLEDYI VDMENRSAM DHSQPPTED VWTQLQQKE 
      NDLLLAAEL GKALLEKNE ELKKQHDAV IEDYSKKLE 
      AVEQDRHLL KRKLANKES EFEGRILEL QNDITELSN 
      KLATKESVL KQWDRDKSN LVAELNAQN ARLTSQLKE 
      AAAVESQLQ QQIECLKQE CAVGKTNLH EHMSSVDTL 
      RDELDLVTE KNKELERRL HMAAAERDS LANALEEAS 
      DRILLLERH AREQDLRYQ HSLKDYSLP QEKLSVEDR 
      LNAGEQRSL LAEMDISEP TLSQECMSV YRQLRSLVQ 
      QLKSHNDDD SGLHSDCST TSFEENAQF SAGLLSEVA 
      QELVGLVLD TDVVRLLER LEQARREIQ ERDAELARR 
      SEKIMELST KVSVCEVEL QAAMEERDR ARNDASNSS 
      LAQEDVVIK AREDRDLAV QRKTKAEVE LAKTRVELM 
      QANSQLLEA IQQKVELSQ QLEQWQMDM QELLDEQLR 
      TKLTSQEVS MKQIVHTPV APPRRKLLS FFLR
    • >TC030908 RB
      MLKSLEDYI VDMENRSAM DHSQPPTED VWTQLQQKE 
      NDLLLAAEL GKALLEKNE ELKKQHDAV IEDYSKKLE 
      VCYVSVKGD KLFSYGRLI LL
    • >TC030908 RC
      MNAVEQDRH LLKRKLANK ESEFEGRIL ELQNDITEL 
      SNKLATKES VLKQWDRDK SNLVAELNA QNARLTSQL 
      KEAAAVESQ LQQQIECLK QECAVGKTN LHEHMSSVD 
      TLRDELDLV TEKNKELER RLHMAAAER DSLANALEE 
      ASDRILLLE RHAREQDLR YQHSLKDYS LPQEKLSVE 
      DRLNAGEQR SLLAEMDIS EPTLSQECM SVYRQLRSL 
      VQQLKSHND DDSGLHSDC STTSFEENA QFSAGLLSE 
      VAQELVGLV LDTDVVRLL ERLEQARRE IQERDAELA 
      RRSEKIMEL STKVSVCEV ELQAAMEER DRARNDASN 
      SSLAQEDVV IKAREDRDL AVQRKTKAE VELAKTRVE 
      LMQANSQLL EAIQQKVEL SQQLEQWQM DMQELLDEQ 
      LRTKLTSQE VSMKQIVHT PVAPPRRKL LSFFLR
    • >TC030908 RD
      MNAVEQDRH LLKRKLANK ESEFEGRIL ELQNDITEL 
      SNKLATKES VLKQWDRDK SNLVAELNA QNARLTSQL 
      KEAAAVESQ LQQQIECLK QECAVGKTN LHEHMSSVD 
      TLRDELDLV TEKNKELER RLHMAAAER DSLANALEE 
      ASDRILLLE RHAREQDLR YQHSLKDYS LPQEKLSVE 
      DRLNAEMDI SEPTLSQEC MSVYRQLRS LVQQLKSHN 
      DDDSGLHSD CSTTSFEEN AQFSAGLLS EVAQELVGL 
      VLDTDVVRL LERLEQARR EIQERDAEL ARRSEKIME 
      LSTKVSVCE VELQAAMEE RDRARNDAS NSSLAQEDV 
      VIKAREDRD LAVQRKTKA EVELAKTRV ELMQANSQL 
      LEAIQQKVE LSQQLEQWQ MDMQELLDE QLRTKLTSQ 
      EVSMKQIVH TPVAPPRRK LLSFFLR
    • >TC030908 RE
      MNAVEQDRH LLKRKLANK ESEFEGRIL ELQNDITEL 
      SNKLATKES VLKQWDRDK SNLVAELNA QNARLTSQL 
      KEAAAVESQ LQQQIECLK QECAVGKTN LHEHMSSVD 
      TLRDELDLV TEKNKELER RLHMAAAER DSLANALEE 
      ASDRILLLE RHAREQDLR YQHSLKDYS LPQEKLSVE 
      DRLNEMDIS EPTLSQECM SVYRQLRSL VQQLKSHND 
      DDSGLHSDC STTSFEENA QFSAGLLSE VAQELVGLV 
      LDTDVVRLL ERLEQARRE IQERDAELA RRSEKIMEL 
      STKVSVCEV ELQAAMEER DRARNDASN SSLAQEDVV 
      IKAREDRDL AVQRKTKAE VELAKTRVE LMQANSQLL 
      EAIQQKVEL SQQLEQWQM DMQELLDEQ LRTKLTSQE 
      VSMKQIVHT PVAPPRRKL LSFFLR
    CDS sequence
    • >TC030908 RA
      atgctgaaa tccctagaa gattacata gtagatatg 
      gaaaatcgt agtgccatg gaccactcc caacccccc 
      acagaagac gtctggacc caattacag caaaaggaa 
      aacgatctc ttactagcc gccgaactc ggcaaagcc 
      cttttggag aaaaacgaa gaactgaaa aagcagcac 
      gacgccgtc attgaggac tattcgaaa aagttggag 
      gctgtcgag caagaccgt catctccta aaacgtaaa 
      ctagccaat aaagaatcg gaattcgaa gggcgcatt 
      ctcgaatta caaaacgac ataaccgaa ctatcgaat 
      aaattagca acgaaagaa tctgtactt aaacaatgg 
      gacagagac aaaagcaat ctcgtggcg gaactgaac 
      gctcaaaac gcccgtctt acatcccaa ctgaaagaa 
      gcagcagct gttgaaagc caattgcaa caacaaatc 
      gaatgttta aaacaagag tgtgccgtt ggcaaaacg 
      aatctccac gaacatatg agcagtgtt gacactcta 
      agagatgag ttagatttg gttacggaa aaaaataaa 
      gaactggag aggagactg cacatggct gcggcggaa 
      cgagatagt ttagccaat gccttagaa gaagcttct 
      gatagaatt ttgctgttg gagagacat gccagggag 
      caagactta aggtaccag cactcgctg aaagactat 
      tccttgcca caggagaaa ttatccgtt gaagataga 
      ctgaatgcc ggtgaacaa cgatctctc ttagcagaa 
      atggatata agcgaaccg actttatcg caagaatgt 
      atgtccgtc taccgccag ttgagatct ttggtccaa 
      caactcaaa tcgcacaac gacgatgac agcggatta 
      cactccgac tgttccacc acatcgttt gaagaaaac 
      gcccaattt tcagcggga cttttgagc gaagttgcc 
      caagaacta gtcggctta gttttagat actgatgtt 
      gtgcggttg cttgagagg cttgaacaa gcgagaagg 
      gaaatacaa gaaagggat gcggagctg gcaaggagg 
      agcgagaaa attatggag ctgagcact aaagtttca 
      gtctgtgaa gtggaattg caagcagcg atggaggaa 
      cgagatagg gcgagaaac gacgcttca aattcttcg 
      ctggctcaa gaagatgtc gtgattaaa gcgagagaa 
      gaccgggat ttggccgtc caacgaaaa acaaaggct 
      gaagtcgag ttggctaag accagagtc gagttgatg 
      caagccaac agccagtta ctggaagcc atccagcaa 
      aaagtggaa ttaagtcaa caactcgag cagtggcag 
      atggacatg caggagcta ctggacgaa cagttgcgc 
      accaaactc acttcgcag gaagtcagc atgaagcaa 
      atcgtgcac acgccagtg gcgccccct cgaagaaaa 
      ctcctgagt ttcttcctt cgatag
    • >TC030908 RB
      atgctgaaa tccctagaa gattacata gtagatatg 
      gaaaatcgt agtgccatg gaccactcc caacccccc 
      acagaagac gtctggacc caattacag caaaaggaa 
      aacgatctc ttactagcc gccgaactc ggcaaagcc 
      cttttggag aaaaacgaa gaactgaaa aagcagcac 
      gacgccgtc attgaggac tattcgaaa aagttggag 
      gtatgttat gtttcagtg aaaggtgac aaattattc 
      agttacgga cgattaatt ctcctgtaa
    • >TC030908 RC
      atgaacgct gtcgagcaa gaccgtcat ctcctaaaa 
      cgtaaacta gccaataaa gaatcggaa ttcgaaggg 
      cgcattctc gaattacaa aacgacata accgaacta 
      tcgaataaa ttagcaacg aaagaatct gtacttaaa 
      caatgggac agagacaaa agcaatctc gtggcggaa 
      ctgaacgct caaaacgcc cgtcttaca tcccaactg 
      aaagaagca gcagctgtt gaaagccaa ttgcaacaa 
      caaatcgaa tgtttaaaa caagagtgt gccgttggc 
      aaaacgaat ctccacgaa catatgagc agtgttgac 
      actctaaga gatgagtta gatttggtt acggaaaaa 
      aataaagaa ctggagagg agactgcac atggctgcg 
      gcggaacga gatagttta gccaatgcc ttagaagaa 
      gcttctgat agaattttg ctgttggag agacatgcc 
      agggagcaa gacttaagg taccagcac tcgctgaaa 
      gactattcc ttgccacag gagaaatta tccgttgaa 
      gatagactg aatgccggt gaacaacga tctctctta 
      gcagaaatg gatataagc gaaccgact ttatcgcaa 
      gaatgtatg tccgtctac cgccagttg agatctttg 
      gtccaacaa ctcaaatcg cacaacgac gatgacagc 
      ggattacac tccgactgt tccaccaca tcgtttgaa 
      gaaaacgcc caattttca gcgggactt ttgagcgaa 
      gttgcccaa gaactagtc ggcttagtt ttagatact 
      gatgttgtg cggttgctt gagaggctt gaacaagcg 
      agaagggaa atacaagaa agggatgcg gagctggca 
      aggaggagc gagaaaatt atggagctg agcactaaa 
      gtttcagtc tgtgaagtg gaattgcaa gcagcgatg 
      gaggaacga gatagggcg agaaacgac gcttcaaat 
      tcttcgctg gctcaagaa gatgtcgtg attaaagcg 
      agagaagac cgggatttg gccgtccaa cgaaaaaca 
      aaggctgaa gtcgagttg gctaagacc agagtcgag 
      ttgatgcaa gccaacagc cagttactg gaagccatc 
      cagcaaaaa gtggaatta agtcaacaa ctcgagcag 
      tggcagatg gacatgcag gagctactg gacgaacag 
      ttgcgcacc aaactcact tcgcaggaa gtcagcatg 
      aagcaaatc gtgcacacg ccagtggcg ccccctcga 
      agaaaactc ctgagtttc ttccttcga tag
    • >TC030908 RD
      atgaacgct gtcgagcaa gaccgtcat ctcctaaaa 
      cgtaaacta gccaataaa gaatcggaa ttcgaaggg 
      cgcattctc gaattacaa aacgacata accgaacta 
      tcgaataaa ttagcaacg aaagaatct gtacttaaa 
      caatgggac agagacaaa agcaatctc gtggcggaa 
      ctgaacgct caaaacgcc cgtcttaca tcccaactg 
      aaagaagca gcagctgtt gaaagccaa ttgcaacaa 
      caaatcgaa tgtttaaaa caagagtgt gccgttggc 
      aaaacgaat ctccacgaa catatgagc agtgttgac 
      actctaaga gatgagtta gatttggtt acggaaaaa 
      aataaagaa ctggagagg agactgcac atggctgcg 
      gcggaacga gatagttta gccaatgcc ttagaagaa 
      gcttctgat agaattttg ctgttggag agacatgcc 
      agggagcaa gacttaagg taccagcac tcgctgaaa 
      gactattcc ttgccacag gagaaatta tccgttgaa 
      gatagactg aatgcagaa atggatata agcgaaccg 
      actttatcg caagaatgt atgtccgtc taccgccag 
      ttgagatct ttggtccaa caactcaaa tcgcacaac 
      gacgatgac agcggatta cactccgac tgttccacc 
      acatcgttt gaagaaaac gcccaattt tcagcggga 
      cttttgagc gaagttgcc caagaacta gtcggctta 
      gttttagat actgatgtt gtgcggttg cttgagagg 
      cttgaacaa gcgagaagg gaaatacaa gaaagggat 
      gcggagctg gcaaggagg agcgagaaa attatggag 
      ctgagcact aaagtttca gtctgtgaa gtggaattg 
      caagcagcg atggaggaa cgagatagg gcgagaaac 
      gacgcttca aattcttcg ctggctcaa gaagatgtc 
      gtgattaaa gcgagagaa gaccgggat ttggccgtc 
      caacgaaaa acaaaggct gaagtcgag ttggctaag 
      accagagtc gagttgatg caagccaac agccagtta 
      ctggaagcc atccagcaa aaagtggaa ttaagtcaa 
      caactcgag cagtggcag atggacatg caggagcta 
      ctggacgaa cagttgcgc accaaactc acttcgcag 
      gaagtcagc atgaagcaa atcgtgcac acgccagtg 
      gcgccccct cgaagaaaa ctcctgagt ttcttcctt 
      cgatag
    • >TC030908 RE
      atgaacgct gtcgagcaa gaccgtcat ctcctaaaa 
      cgtaaacta gccaataaa gaatcggaa ttcgaaggg 
      cgcattctc gaattacaa aacgacata accgaacta 
      tcgaataaa ttagcaacg aaagaatct gtacttaaa 
      caatgggac agagacaaa agcaatctc gtggcggaa 
      ctgaacgct caaaacgcc cgtcttaca tcccaactg 
      aaagaagca gcagctgtt gaaagccaa ttgcaacaa 
      caaatcgaa tgtttaaaa caagagtgt gccgttggc 
      aaaacgaat ctccacgaa catatgagc agtgttgac 
      actctaaga gatgagtta gatttggtt acggaaaaa 
      aataaagaa ctggagagg agactgcac atggctgcg 
      gcggaacga gatagttta gccaatgcc ttagaagaa 
      gcttctgat agaattttg ctgttggag agacatgcc 
      agggagcaa gacttaagg taccagcac tcgctgaaa 
      gactattcc ttgccacag gagaaatta tccgttgaa 
      gatagactg aatgaaatg gatataagc gaaccgact 
      ttatcgcaa gaatgtatg tccgtctac cgccagttg 
      agatctttg gtccaacaa ctcaaatcg cacaacgac 
      gatgacagc ggattacac tccgactgt tccaccaca 
      tcgtttgaa gaaaacgcc caattttca gcgggactt 
      ttgagcgaa gttgcccaa gaactagtc ggcttagtt 
      ttagatact gatgttgtg cggttgctt gagaggctt 
      gaacaagcg agaagggaa atacaagaa agggatgcg 
      gagctggca aggaggagc gagaaaatt atggagctg 
      agcactaaa gtttcagtc tgtgaagtg gaattgcaa 
      gcagcgatg gaggaacga gatagggcg agaaacgac 
      gcttcaaat tcttcgctg gctcaagaa gatgtcgtg 
      attaaagcg agagaagac cgggatttg gccgtccaa 
      cgaaaaaca aaggctgaa gtcgagttg gctaagacc 
      agagtcgag ttgatgcaa gccaacagc cagttactg 
      gaagccatc cagcaaaaa gtggaatta agtcaacaa 
      ctcgagcag tggcagatg gacatgcag gagctactg 
      gacgaacag ttgcgcacc aaactcact tcgcaggaa 
      gtcagcatg aagcaaatc gtgcacacg ccagtggcg 
      ccccctcga agaaaactc ctgagtttc ttccttcga 
      tag
    mRNA sequence
    • >TC030908 RA
      agtgtagta cctccgttc atttgtttt atttttacg 
      ttcgttgtt tggtgttgt gttaaaaat tcttgtgcg 
      cttgtgttg tcaaaattt tggaaattt ttggatttt 
      gcaaaagat gtgtcctga aaaatgagt atcaaaatg 
      gtgccttat ccctaccgt cccaggatg atcgaccgg 
      cttcgaacc ctgtaattt cagcaatgc tgaaatccc 
      tagaagatt acatagtag atatggaaa atcgtagtg 
      ccatggacc actcccaac cccccacag aagacgtct 
      ggacccaat tacagcaaa aggaaaacg atctcttac 
      tagccgccg aactcggca aagcccttt tggagaaaa 
      acgaagaac tgaaaaagc agcacgacg ccgtcattg 
      aggactatt cgaaaaagt tggaggctg tcgagcaag 
      accgtcatc tcctaaaac gtaaactag ccaataaag 
      aatcggaat tcgaagggc gcattctcg aattacaaa 
      acgacataa ccgaactat cgaataaat tagcaacga 
      aagaatctg tacttaaac aatgggaca gagacaaaa 
      gcaatctcg tggcggaac tgaacgctc aaaacgccc 
      gtcttacat cccaactga aagaagcag cagctgttg 
      aaagccaat tgcaacaac aaatcgaat gtttaaaac 
      aagagtgtg ccgttggca aaacgaatc tccacgaac 
      atatgagca gtgttgaca ctctaagag atgagttag 
      atttggtta cggaaaaaa ataaagaac tggagagga 
      gactgcaca tggctgcgg cggaacgag atagtttag 
      ccaatgcct tagaagaag cttctgata gaattttgc 
      tgttggaga gacatgcca gggagcaag acttaaggt 
      accagcact cgctgaaag actattcct tgccacagg 
      agaaattat ccgttgaag atagactga atgccggtg 
      aacaacgat ctctcttag cagaaatgg atataagcg 
      aaccgactt tatcgcaag aatgtatgt ccgtctacc 
      gccagttga gatctttgg tccaacaac tcaaatcgc 
      acaacgacg atgacagcg gattacact ccgactgtt 
      ccaccacat cgtttgaag aaaacgccc aattttcag 
      cgggacttt tgagcgaag ttgcccaag aactagtcg 
      gcttagttt tagatactg atgttgtgc ggttgcttg 
      agaggcttg aacaagcga gaagggaaa tacaagaaa 
      gggatgcgg agctggcaa ggaggagcg agaaaatta 
      tggagctga gcactaaag tttcagtct gtgaagtgg 
      aattgcaag cagcgatgg aggaacgag atagggcga 
      gaaacgacg cttcaaatt cttcgctgg ctcaagaag 
      atgtcgtga ttaaagcga gagaagacc gggatttgg 
      ccgtccaac gaaaaacaa aggctgaag tcgagttgg 
      ctaagacca gagtcgagt tgatgcaag ccaacagcc 
      agttactgg aagccatcc agcaaaaag tggaattaa 
      gtcaacaac tcgagcagt ggcagatgg acatgcagg 
      agctactgg acgaacagt tgcgcacca aactcactt 
      cgcaggaag tcagcatga agcaaatcg tgcacacgc 
      cagtggcgc cccctcgaa gaaaactcc tgagtttct 
      tccttcgat agcattcca acaagtggg agaattttg 
      ccgaattca ctttgcacg gatctgagt ttttttgca 
      acgtggtgc agttctaca ttttcgttt tattttgga 
      ttgcgtttg tgatttttc tttgtagtt ttaaaatgt 
      tgctttttt tcgttgtga tctgtcgaa tcgcacttt 
      gatttctgc aaattctcc accgaataa ttttaagca 
      agatcctgt gatttaaag agtttttta agtattgta 
      gttatttac atagagatt tttaattat ttttagatc 
      taacttagt taccaaaac acgttttgg tctttaaaa 
      gtcaatctc aagtgcatt taatccgtc cgatgctaa 
      ttaatgctg ttaccttga tttaataaa ttgtaaata 
      gacta
    • >TC030908 RB
      agtgtagta cctccgttc atttgtttt atttttacg 
      ttcgttgtt tggtgttgt gttaaaaat tcttgtgcg 
      cttgtgttg tcaaaattt tggaaattt ttggatttt 
      gcaaaagat gtgtcctga aaaatgagt atcaaaatg 
      gtgccttat ccctaccgt cccaggatg atcgaccgg 
      cttcgaacc ctgtaattt cagcaatgc tgaaatccc 
      tagaagatt acatagtag atatggaaa atcgtagtg 
      ccatggacc actcccaac cccccacag aagacgtct 
      ggacccaat tacagcaaa aggaaaacg atctcttac 
      tagccgccg aactcggca aagcccttt tggagaaaa 
      acgaagaac tgaaaaagc agcacgacg ccgtcattg 
      aggactatt cgaaaaagt tggaggtat gttatgttt 
      cagtgaaag gtgacaaat tattcagtt acggacgat 
      taattctcc tgtaataat aatatcaag tggtgctag 
      attaaatcg cgtattgcg ga
    • >TC030908 RC
      tgtggtttt ctctttcta gcatgaacg ctgtcgagc 
      aagaccgtc atctcctaa aacgtaaac tagccaata 
      aagaatcgg aattcgaag ggcgcattc tcgaattac 
      aaaacgaca taaccgaac tatcgaata aattagcaa 
      cgaaagaat ctgtactta aacaatggg acagagaca 
      aaagcaatc tcgtggcgg aactgaacg ctcaaaacg 
      cccgtctta catcccaac tgaaagaag cagcagctg 
      ttgaaagcc aattgcaac aacaaatcg aatgtttaa 
      aacaagagt gtgccgttg gcaaaacga atctccacg 
      aacatatga gcagtgttg acactctaa gagatgagt 
      tagatttgg ttacggaaa aaaataaag aactggaga 
      ggagactgc acatggctg cggcggaac gagatagtt 
      tagccaatg ccttagaag aagcttctg atagaattt 
      tgctgttgg agagacatg ccagggagc aagacttaa 
      ggtaccagc actcgctga aagactatt ccttgccac 
      aggagaaat tatccgttg aagatagac tgaatgccg 
      gtgaacaac gatctctct tagcagaaa tggatataa 
      gcgaaccga ctttatcgc aagaatgta tgtccgtct 
      accgccagt tgagatctt tggtccaac aactcaaat 
      cgcacaacg acgatgaca gcggattac actccgact 
      gttccacca catcgtttg aagaaaacg cccaatttt 
      cagcgggac ttttgagcg aagttgccc aagaactag 
      tcggcttag ttttagata ctgatgttg tgcggttgc 
      ttgagaggc ttgaacaag cgagaaggg aaatacaag 
      aaagggatg cggagctgg caaggagga gcgagaaaa 
      ttatggagc tgagcacta aagtttcag tctgtgaag 
      tggaattgc aagcagcga tggaggaac gagataggg 
      cgagaaacg acgcttcaa attcttcgc tggctcaag 
      aagatgtcg tgattaaag cgagagaag accgggatt 
      tggccgtcc aacgaaaaa caaaggctg aagtcgagt 
      tggctaaga ccagagtcg agttgatgc aagccaaca 
      gccagttac tggaagcca tccagcaaa aagtggaat 
      taagtcaac aactcgagc agtggcaga tggacatgc 
      aggagctac tggacgaac agttgcgca ccaaactca 
      cttcgcagg aagtcagca tgaagcaaa tcgtgcaca 
      cgccagtgg cgccccctc gaagaaaac tcctgagtt 
      tcttccttc gatagcatt ccaacaagt gggagaatt 
      ttgccgaat tcactttgc acggatctg agttttttt 
      gcaacgtgg tgcagttct acattttcg ttttatttt 
      ggattgcgt ttgtgattt ttctttgta gttttaaaa 
      tgttgcttt ttttcgttg tgatctgtc gaatcgcac 
      tttgatttc tgcaaattc tccaccgaa taattttaa 
      gcaagatcc tgtgattta aagagtttt ttaagtatt 
      gtagttatt tacatagag atttttaat tatttttag 
      atctaactt agttaccaa aacacgttt tggtcttta 
      aaagtcaat ctcaagtgc atttaatcc gtccgatgc 
      taattaatg ctgttacct tgatttaat aaattgtaa 
      atagacta
    • >TC030908 RD
      tgtggtttt ctctttcta gcatgaacg ctgtcgagc 
      aagaccgtc atctcctaa aacgtaaac tagccaata 
      aagaatcgg aattcgaag ggcgcattc tcgaattac 
      aaaacgaca taaccgaac tatcgaata aattagcaa 
      cgaaagaat ctgtactta aacaatggg acagagaca 
      aaagcaatc tcgtggcgg aactgaacg ctcaaaacg 
      cccgtctta catcccaac tgaaagaag cagcagctg 
      ttgaaagcc aattgcaac aacaaatcg aatgtttaa 
      aacaagagt gtgccgttg gcaaaacga atctccacg 
      aacatatga gcagtgttg acactctaa gagatgagt 
      tagatttgg ttacggaaa aaaataaag aactggaga 
      ggagactgc acatggctg cggcggaac gagatagtt 
      tagccaatg ccttagaag aagcttctg atagaattt 
      tgctgttgg agagacatg ccagggagc aagacttaa 
      ggtaccagc actcgctga aagactatt ccttgccac 
      aggagaaat tatccgttg aagatagac tgaatgcag 
      aaatggata taagcgaac cgactttat cgcaagaat 
      gtatgtccg tctaccgcc agttgagat ctttggtcc 
      aacaactca aatcgcaca acgacgatg acagcggat 
      tacactccg actgttcca ccacatcgt ttgaagaaa 
      acgcccaat tttcagcgg gacttttga gcgaagttg 
      cccaagaac tagtcggct tagttttag atactgatg 
      ttgtgcggt tgcttgaga ggcttgaac aagcgagaa 
      gggaaatac aagaaaggg atgcggagc tggcaagga 
      ggagcgaga aaattatgg agctgagca ctaaagttt 
      cagtctgtg aagtggaat tgcaagcag cgatggagg 
      aacgagata gggcgagaa acgacgctt caaattctt 
      cgctggctc aagaagatg tcgtgatta aagcgagag 
      aagaccggg atttggccg tccaacgaa aaacaaagg 
      ctgaagtcg agttggcta agaccagag tcgagttga 
      tgcaagcca acagccagt tactggaag ccatccagc 
      aaaaagtgg aattaagtc aacaactcg agcagtggc 
      agatggaca tgcaggagc tactggacg aacagttgc 
      gcaccaaac tcacttcgc aggaagtca gcatgaagc 
      aaatcgtgc acacgccag tggcgcccc ctcgaagaa 
      aactcctga gtttcttcc ttcgatagc attccaaca 
      agtgggaga attttgccg aattcactt tgcacggat 
      ctgagtttt tttgcaacg tggtgcagt tctacattt 
      tcgttttat tttggattg cgtttgtga tttttcttt 
      gtagtttta aaatgttgc tttttttcg ttgtgatct 
      gtcgaatcg cactttgat ttctgcaaa ttctccacc 
      gaataattt taagcaaga tcctgtgat ttaaagagt 
      tttttaagt attgtagtt atttacata gagattttt 
      aattatttt tagatctaa cttagttac caaaacacg 
      ttttggtct ttaaaagtc aatctcaag tgcatttaa 
      tccgtccga tgctaatta atgctgtta ccttgattt 
      aataaattg taaatagac ta
    • >TC030908 RE
      tgtggtttt ctctttcta gcatgaacg ctgtcgagc 
      aagaccgtc atctcctaa aacgtaaac tagccaata 
      aagaatcgg aattcgaag ggcgcattc tcgaattac 
      aaaacgaca taaccgaac tatcgaata aattagcaa 
      cgaaagaat ctgtactta aacaatggg acagagaca 
      aaagcaatc tcgtggcgg aactgaacg ctcaaaacg 
      cccgtctta catcccaac tgaaagaag cagcagctg 
      ttgaaagcc aattgcaac aacaaatcg aatgtttaa 
      aacaagagt gtgccgttg gcaaaacga atctccacg 
      aacatatga gcagtgttg acactctaa gagatgagt 
      tagatttgg ttacggaaa aaaataaag aactggaga 
      ggagactgc acatggctg cggcggaac gagatagtt 
      tagccaatg ccttagaag aagcttctg atagaattt 
      tgctgttgg agagacatg ccagggagc aagacttaa 
      ggtaccagc actcgctga aagactatt ccttgccac 
      aggagaaat tatccgttg aagatagac tgaatgaaa 
      tggatataa gcgaaccga ctttatcgc aagaatgta 
      tgtccgtct accgccagt tgagatctt tggtccaac 
      aactcaaat cgcacaacg acgatgaca gcggattac 
      actccgact gttccacca catcgtttg aagaaaacg 
      cccaatttt cagcgggac ttttgagcg aagttgccc 
      aagaactag tcggcttag ttttagata ctgatgttg 
      tgcggttgc ttgagaggc ttgaacaag cgagaaggg 
      aaatacaag aaagggatg cggagctgg caaggagga 
      gcgagaaaa ttatggagc tgagcacta aagtttcag 
      tctgtgaag tggaattgc aagcagcga tggaggaac 
      gagataggg cgagaaacg acgcttcaa attcttcgc 
      tggctcaag aagatgtcg tgattaaag cgagagaag 
      accgggatt tggccgtcc aacgaaaaa caaaggctg 
      aagtcgagt tggctaaga ccagagtcg agttgatgc 
      aagccaaca gccagttac tggaagcca tccagcaaa 
      aagtggaat taagtcaac aactcgagc agtggcaga 
      tggacatgc aggagctac tggacgaac agttgcgca 
      ccaaactca cttcgcagg aagtcagca tgaagcaaa 
      tcgtgcaca cgccagtgg cgccccctc gaagaaaac 
      tcctgagtt tcttccttc gatagcatt ccaacaagt 
      gggagaatt ttgccgaat tcactttgc acggatctg 
      agttttttt gcaacgtgg tgcagttct acattttcg 
      ttttatttt ggattgcgt ttgtgattt ttctttgta 
      gttttaaaa tgttgcttt ttttcgttg tgatctgtc 
      gaatcgcac tttgatttc tgcaaattc tccaccgaa 
      taattttaa gcaagatcc tgtgattta aagagtttt 
      ttaagtatt gtagttatt tacatagag atttttaat 
      tatttttag atctaactt agttaccaa aacacgttt 
      tggtcttta aaagtcaat ctcaagtgc atttaatcc 
      gtccgatgc taattaatg ctgttacct tgatttaat 
      aaattgtaa atagacta
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