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Gene information
General information
TC015681 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4992812..4994957
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015681
    iBeetle screen iB_05868

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015681 RA
      MDQLKGKVV NVVLTIQEG KGMDSIKNL TLISANFNG 
      RILESDPVN AEDCPAFNT NLIWEIEKK DLRKIRSSN 
      QPLRVECLS VDNQNRREK FGHVLLDLR SAQIVEEPD 
      RQVSFRWHK LLGVRNKKR NCVPELYVS LTIRNHLEV 
      QPQESSDDI WEPEPRAEG SIPIKYLED GYIVVGDPL 
      KCTEDYSLN IHIKSASSL DLLLPEVLV FHQNNNKYC 
      MCFKIFGIP IKTKQFSKD LHDKIIFEE RIVVRLLSN 
      ITVLRQFFS EEKFEVGFN CGNDLLGLT TVSFDDLSN 
      LNEKLLLPA CFFRLPSPN GIVPVGNDG RKPFLSLEI 
      YLVKNREEV PALVSNLEP DVSKACGDY TEEAKQQIS 
      QQLLLITEE HMKELEEWK ERQKVEFEE QLQNLKESE 
      LEKDREEIE RKKAELNEN IIKCKELQE ELQEKLDEL 
      KLSKTLKKK RKSSTSLVS VIAENQKKF ADCDKELLI 
      DYISTLQSD NENLKQIIS EQKQELENI EKTALTKEQ 
      TTNLLQELK GLEEKFEVA QQTKTYFKE QWQRACQEI 
      HNLKTEDYK QLQNQLRER REELDHLCI LDEPTELVS 
      F
    CDS sequence
    • >TC015681 RA
      atggatcaa cttaaagga aaagtagta aatgtggtc 
      ctcacaatt caggaggga aaagggatg gattccatt 
      aaaaattta acgttgatt tctgcaaac tttaacgga 
      agaatccta gaatcggat ccagtcaac gctgaagac 
      tgtcctgct ttcaacaca aacttaatt tgggaaatt 
      gagaagaaa gacttgaga aaaatccgc agctcgaat 
      cagcctctc cgagtcgaa tgtttgagc gttgataat 
      caaaatcgc cgagagaaa ttcggccac gttttgctc 
      gatctacgc agcgcccaa attgtcgaa gagccagac 
      cggcaggtg tccttcagg tggcacaaa ctgctagga 
      gttcgcaac aaaaaacga aactgtgtc cccgagctg 
      tacgtgtct cttacaata cgtaaccac ttggaggta 
      caaccacag gagtcgtct gacgatatc tgggaacct 
      gaacccaga gctgaaggt tcaattcct atcaaatat 
      ctggaagat gggtacatt gtggtgggg gacccattg 
      aaatgtacc gaagattac tcgcttaat atccacatt 
      aaatcggcg tcaagtctc gatttgttg ctaccggaa 
      gttttagtt ttccaccaa aataataac aagtactgc 
      atgtgtttt aaaattttt ggcattccg atcaaaacc 
      aaacagttt tcgaaagat ttacacgac aaaatcatt 
      tttgaggag agaattgtt gtgaggttg ctttcaaat 
      atcacggtg ctgagacaa ttctttagt gaggaaaag 
      tttgaggtg ggtttcaac tgcggcaat gatcttctt 
      ggtttgaca actgttagc tttgatgat ttgagtaat 
      ttgaacgaa aagctactt ttgcctgca tgtttcttc 
      agacttccg tcacctaat ggtatcgta cctgttggt 
      aatgacggt cgaaaaccc tttctctcg cttgaaatt 
      tatctggta aaaaaccga gaagaggta cctgctttg 
      gtttctaat ttagagccg gatgtttcg aaggcttgt 
      ggtgattac acggaagaa gccaaacaa caaataagt 
      caacagttg ttgcttatt actgaggag catatgaaa 
      gaactggag gaatggaaa gaaagacag aaagttgag 
      tttgaggaa caactccag aatttgaaa gaaagtgag 
      ttggaaaaa gatcgtgag gagatagaa cgtaaaaag 
      gccgaacta aacgaaaac ataataaaa tgcaaggaa 
      ctacaagag gagcttcag gagaaactc gatgaattg 
      aaactgtca aaaacattg aagaaaaaa cgcaagtcg 
      tcaacaagc ctagtttcg gtgatcgcc gaaaatcaa 
      aagaaattt gccgactgt gataaagaa ttgttgatt 
      gattacatc agcacatta caaagtgat aacgaaaat 
      ttgaagcaa attatcagc gaacagaag caagaattg 
      gagaatatc gagaagact gctttaacc aaagaacaa 
      acaaccaac cttttgcaa gaattgaaa ggtttggaa 
      gaaaaattc gaagtggca caacagaca aaaacctat 
      ttcaaggaa cagtggcag agagcttgc caagaaatt 
      cacaatttg aagacagaa gactacaaa caactccaa 
      aatcagttg cgggaacgc agggaagag ttggatcat 
      ctttgtatt ctagacgaa ccaactgaa ctcgtgtca 
      ttttga
    mRNA sequence
    • >TC015681 RA
      aaagttgtt gtttttgtg tcaaaattt tgacaagat 
      tttaaaaga tggatcaac ttaaaggaa aagtagtaa 
      atgtggtcc tcacaattc aggagggaa aagggatgg 
      attccatta aaaatttaa cgttgattt ctgcaaact 
      ttaacggaa gaatcctag aatcggatc cagtcaacg 
      ctgaagact gtcctgctt tcaacacaa acttaattt 
      gggaaattg agaagaaag acttgagaa aaatccgca 
      gctcgaatc agcctctcc gagtcgaat gtttgagcg 
      ttgataatc aaaatcgcc gagagaaat tcggccacg 
      ttttgctcg atctacgca gcgcccaaa ttgtcgaag 
      agccagacc ggcaggtgt ccttcaggt ggcacaaac 
      tgctaggag ttcgcaaca aaaaacgaa actgtgtcc 
      ccgagctgt acgtgtctc ttacaatac gtaaccact 
      tggaggtac aaccacagg agtcgtctg acgatatct 
      gggaacctg aacccagag ctgaaggtt caattccta 
      tcaaatatc tggaagatg ggtacattg tggtggggg 
      acccattga aatgtaccg aagattact cgcttaata 
      tccacatta aatcggcgt caagtctcg atttgttgc 
      taccggaag ttttagttt tccaccaaa ataataaca 
      agtactgca tgtgtttta aaatttttg gcattccga 
      tcaaaacca aacagtttt cgaaagatt tacacgaca 
      aaatcattt ttgaggaga gaattgttg tgaggttgc 
      tttcaaata tcacggtgc tgagacaat tctttagtg 
      aggaaaagt ttgaggtgg gtttcaact gcggcaatg 
      atcttcttg gtttgacaa ctgttagct ttgatgatt 
      tgagtaatt tgaacgaaa agctacttt tgcctgcat 
      gtttcttca gacttccgt cacctaatg gtatcgtac 
      ctgttggta atgacggtc gaaaaccct ttctctcgc 
      ttgaaattt atctggtaa aaaaccgag aagaggtac 
      ctgctttgg tttctaatt tagagccgg atgtttcga 
      aggcttgtg gtgattaca cggaagaag ccaaacaac 
      aaataagtc aacagttgt tgcttatta ctgaggagc 
      atatgaaag aactggagg aatggaaag aaagacaga 
      aagttgagt ttgaggaac aactccaga atttgaaag 
      aaagtgagt tggaaaaag atcgtgagg agatagaac 
      gtaaaaagg ccgaactaa acgaaaaca taataaaat 
      gcaaggaac tacaagagg agcttcagg agaaactcg 
      atgaattga aactgtcaa aaacattga agaaaaaac 
      gcaagtcgt caacaagcc tagtttcgg tgatcgccg 
      aaaatcaaa agaaatttg ccgactgtg ataaagaat 
      tgttgattg attacatca gcacattac aaagtgata 
      acgaaaatt tgaagcaaa ttatcagcg aacagaagc 
      aagaattgg agaatatcg agaagactg ctttaacca 
      aagaacaaa caaccaacc ttttgcaag aattgaaag 
      gtttggaag aaaaattcg aagtggcac aacagacaa 
      aaacctatt tcaaggaac agtggcaga gagcttgcc 
      aagaaattc acaatttga agacagaag actacaaac 
      aactccaaa atcagttgc gggaacgca gggaagagt 
      tggatcatc tttgtattc tagacgaac caactgaac 
      tcgtgtcat tttgaaaat aaaggggat catgtttaa 
      atttatttt tctttattc ttaaacaac ttgaaatct 
      acaattgtc aatccggct aacagtatt tcttttgct 
      tgatgtgtc gagcttgcg gtaaaaagc acttggaaa 
      ctgtgtgca atttggttg aagcacctg gcaagtcca 
      accgttgtt gaagcattt ctttggctt ctttcctac 
      tccaaggac gttgtacaa catcgataa catccgaaa 
      ccatctcat gctcaatac atctgaaac caaaactga 
      aaatctact aaccaatca gtttccaaa atatacaat 
      gagcaagga tcagctctc acaatcctc tcaattgga 
      taaataaac gtggtggtg tcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY