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Gene information
General information
TC015676 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5127059..5145561
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015676
    iBeetle screen iB_05867

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015676 RA
      MGQRRLRHI STRLHWRYP QVQLFSTYC AYLPVIINP 
      VTTMTEKLN PKVQEFQFH SIPKTTKWE SFQKVIYDP 
      STHEIFGRT KKSWYQLLV FYSIFYICL AALFAICMK 
      GLTATLSDK EPKWKLEES LIGTSPGLG FRPISNNTQ 
      EGSLIWYNL KDVQTTQKW VRLVDQFLQ PYKVQQIGK 
      NYQQCDYEI AVKEGHVCA VEVDKFGPC TAENNYGFN 
      STSPCVFLK LNRIFGWVP DYVTQPESE MPDDLKEII 
      TYATSNNKS QVWISCAGE NPFDREHVI GFDYFPSRG 
      FPGYYFPYT NNDNYLSPL IAVQINVKT NVIVNIECR 
      AWARNIAYD GGNNHREGS VHFEILVDS KN
    CDS sequence
    • >TC015676 RA
      atgggtcaa cgtcggctt cgtcacatc agcacgcgc 
      ctccactgg cgttaccca caagttcag cttttcagc 
      acatattgt gcttatctc cctgttatc ataaaccca 
      gtcacaacc atgaccgaa aagttaaac ccaaaagtc 
      caggaattt cagttccat tcgatcccg aaaaccacc 
      aaatgggag agtttccag aaagtaatt tacgaccct 
      tcaacacac gaaattttc ggaagaacg aagaaaagt 
      tggtaccaa ttgctagtt ttctactcg attttctac 
      atatgcctt gccgccctt ttcgcaatt tgcatgaaa 
      ggcttgact gcgacctta agcgacaaa gaaccgaaa 
      tggaagctt gaggaaagc ctaattggg acgagtcca 
      ggactgggt tttcggcca atttccaac aacacgcaa 
      gaaggttcc ctcatttgg tacaactta aaagacgtg 
      caaacaaca caaaagtgg gtacgactt gtggaccag 
      ttcttacaa ccctacaaa gtgcaacaa atcggcaaa 
      aactaccaa caatgcgac tacgaaatt gcggtcaaa 
      gaggggcac gtgtgtgcg gtagaagtg gacaaattc 
      ggaccatgc actgctgaa aacaactac gggttcaat 
      tcaacttcg ccttgtgtt tttctaaaa ttgaaccgg 
      atttttgga tgggttccc gattacgta acgcagcca 
      gaaagtgaa atgcctgat gatttaaaa gagattatt 
      acgtacgcc acttcgaat aataagagc caagtgtgg 
      ataagttgt gcgggagaa aatccgttt gatcgagaa 
      catgtaatt gggttcgat tactttcca agtaggggg 
      tttcccgga tactatttc ccttacact aataacgat 
      aattatctc agtccactt atcgctgta caaataaat 
      gttaaaacg aatgtgatt gtcaatatc gaatgtagg 
      gcttgggct agaaacatc gcctatgat ggcgggaac 
      aaccatcga gagggatcc gttcatttt gagattttg 
      gtggactcc aaaaattga
    mRNA sequence
    • >TC015676 RA
      agggatgaa aacgcgaaa ccctttcag tggcagcga 
      ccgttcgaa cgggggtac gcgttttgc gctacaaaa 
      atcgaacgc gatgagaca tcaataaaa atcacaact 
      aattttttc caaaaattc acactcggg ggtggctgt 
      gagtgaaac gtgtgccaa taaatggat atttctcac 
      ccccaaggc aactcgggt gggcgggcc aacgtcgag 
      aggcggttt ctgccgcca ccagcgagc tagtgggag 
      cattgtgtg agcaaagta tgaagctaa gcgtttcgg 
      cgtatagag cccacgctt ccgcccata agaagatat 
      tggacactg cttatcagc ttaattatg ggtcaacgt 
      cggcttcgt cacatcagc acgcgcctc cactggcgt 
      tacccacaa gttcagctt ttcagcaca tattgtgct 
      tatctccct gttatcata aacccagtc acaaccatg 
      accgaaaag ttaaaccca aaagtccag gaatttcag 
      ttccattcg atcccgaaa accaccaaa tgggagagt 
      ttccagaaa gtaatttac gacccttca acacacgaa 
      attttcgga agaacgaag aaaagttgg taccaattg 
      ctagttttc tactcgatt ttctacata tgccttgcc 
      gcccttttc gcaatttgc atgaaaggc ttgactgcg 
      accttaagc gacaaagaa ccgaaatgg aagcttgag 
      gaaagccta attgggacg agtccagga ctgggtttt 
      cggccaatt tccaacaac acgcaagaa ggttccctc 
      atttggtac aacttaaaa gacgtgcaa acaacacaa 
      aagtgggta cgacttgtg gaccagttc ttacaaccc 
      tacaaagtg caacaaatc ggcaaaaac taccaacaa 
      tgcgactac gaaattgcg gtcaaagag gggcacgtg 
      tgtgcggta gaagtggac aaattcgga ccatgcact 
      gctgaaaac aactacggg ttcaattca acttcgcct 
      tgtgttttt ctaaaattg aaccggatt tttggatgg 
      gttcccgat tacgtaacg cagccagaa agtgaaatg 
      cctgatgat ttaaaagag attattacg tacgccact 
      tcgaataat aagagccaa gtgtggata agttgtgcg 
      ggagaaaat ccgtttgat cgagaacat gtaattggg 
      ttcgattac tttccaagt agggggttt cccggatac 
      tatttccct tacactaat aacgataat tatctcagt 
      ccacttatc gctgtacaa ataaatgtt aaaacgaat 
      gtgattgtc aatatcgaa tgtagggct tgggctaga 
      aacatcgcc tatgatggc gggaacaac catcgagag 
      ggatccgtt cattttgag attttggtg gactccaaa 
      aattgatag aaacgtgta atttatttg ttatttttt 
      gcacaaaat aaagagaca gtaatttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY