Status
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Gene information
General information
TC015667 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4498166..4524150
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015667
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05864

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    posterior terminus present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015667 RA
      MNKHLCVFL LATSTILVF GNDNDYDDY EEDLTPHNA 
SSNSTLVFI RPLANLSID TATKTIRLT CEVQNLDLN 
NTNRVTITW RKNYVPIER SDRIIIRKS SKDNKHRST 
LRINEIEFF DRGFYDCTA SNGVDKIRS QALVDVATD 
SGRVDSTVA LKFDGEITT IPAGTVFDA KLPELPHEK 
ITNTFQMQS QRPYNLSEP FCQVYLGET CKDYLDNQY 
VFVQPPYSQ KDIEQMLSD AFLVISQSN DISSSCNKF 
ARPSLCYSA FPLCTDPKQ INMYQTTKM DRFKLDNVG 
FKNLRNKLG SSLRRICKE DCLLLETEL CSKEYAIAK 
RHPVIGKTL ELEECQFLP NETEVSSRN CLELGVDHE 
SVNKEDTCY WDNGELYRG VQNMSELGN KCLRWAHQF 
HIHLSEHPE LTGHNYCRN PGGVESQPF CYVDGDPIR 
RETCDVTKC TYVVWMYVI GIFIAISVF SVVLFGVFC 
CRRRNKLAR NLQNMNVPV ADKNIYGSG SPTAPPMEL 
NSLLPPNIP PQRNSHSTK NGYQNVPQY TYKEVAFVE 
ELGEGAFGK VYKGELKTK NGKIFVAVK SLKENASAK 
TQADFQREI ELISELKHP NIICLLGVV VKQEPMCML 
FEFMSEGDL HEFLISNSP SEGKSLTQN QFLHIAIQI 
AQGMEYLSD NHYVHRDLA ARNCLVSKD LVVKISDFG 
LSRDMYSCD YYRVQSKSL LPVRWMPPE SILYGKFTT 
ESDVWSYGV VLWEIYSYG LQPYYGYNN QEVINMIRS 
RKLLPCPDA CPSYCYALM VECWAELAV RRPSFGEIS 
HRLRVWKQN GNLNGAYFK TTDSHKGGH SSKSEEDSA 
TWERKRLHE SQSSLTSRS SSLGNKTQS TSLSNEQSR 
NRRSHRRKE DSLDRAMSP KNTIVNSNG DNFEIRITH
    CDS sequence
    • >TC015667 RA
      atgaacaaa cacctctgt gtcttcctg ctcgcaaca 
tcgacgatt cttgttttt ggcaacgac aatgactac 
gacgactat gaagaggac ctaaccccc cacaacgcc 
tcctcaaac tccaccctt gtcttcatc cgccccctt 
gccaaccta tcaatcgac accgccacc aaaaccatt 
cgtctaaca tgcgaagta caaaacctg gacctaaac 
aacacaaac cgtgtcacc atcacgtgg cgcaaaaac 
tacgtcccg atcgaacgt agcgatcgc ataattatt 
cgaaaatca agcaaagac aacaaacat cgttccaca 
ctacgtatc aacgaaatt gagttcttc gatcgtggt 
ttctacgat tgtacggcg tcgaatggc gtcgataaa 
atccgatcg caggcgctt gtggacgtc gcgacggac 
tcgggacgc gtcgacagc accgtggcg ctgaaattc 
gacggcgaa atcacaaca ataccggca gggactgtt 
tttgacgcc aagttgccg gaactgcca cacgaaaaa 
atcaccaac acttttcaa atgcaatcg caacggcct 
tataatctc agtgagccg ttctgtcaa gtgtatcta 
ggggagacg tgcaaagac tacttggat aatcagtat 
gtttttgtg cagcccccg tatagtcaa aaggacatc 
gaacaaatg ctaagtgat gcttttttg gtcatttcg 
cagtcaaac gatatttca tcgagttgt aataaattc 
gcacgtcca agtttgtgt tattcggcg tttcctttg 
tgtaccgat ccgaaacaa ataaatatg tatcagacg 
acgaaaatg gatcggttt aagttagat aatgtcggt 
tttaagaat ttgaggaat aaattgggg agttcgttg 
aggaggatt tgtaaagag gattgtctt ttgttagag 
actgagttg tgtagtaag gaatatgcg attgcgaaa 
cgacatcca gttattgga aagactttg gaattggag 
gagtgtcag tttttgccg aatgagacc gaagtcagt 
tcgagaaat tgtctcgag ttgggtgtc gatcatgaa 
agtgttaat aaagaggat acgtgttat tgggacaac 
ggcgaacta tacaggggt gttcaaaat atgagcgaa 
cttggtaac aaatgttta aggtgggcc caccaattc 
cacatccat ttatccgaa cacccggaa ctgacaggt 
cataattat tgtcgtaat ccaggtggc gttgaatcg 
cagccattt tgttacgtc gatggtgac ccgattcgg 
cgtgaaact tgtgacgtc actaagtgc acctacgtt 
gtttggatg tatgtcatt ggtattttc atagccatc 
tctgttttt agtgtggtt ttatttggg gttttttgt 
tgccgacga agaaacaaa cttgcgcgc aacttacaa 
aatatgaat gttcctgtc gctgataag aatatttat 
ggtagtggg agtcccaca gccccgccc atggaacta 
aacagtctc ctcccacca aatattcct ccacaacgc 
aactcccat tcgaccaaa aacggttat cagaacgtc 
ccccaatat acttacaaa gaagttgct tttgtggag 
gagttgggc gagggtgcg ttcggtaaa gtgtacaaa 
ggtgaattg aaaacaaaa aatgggaaa attttcgtt 
gccgttaag tcattgaaa gaaaatgcg agtgccaaa 
actcaggct gattttcag cgcgagatt gaactgatt 
tcggaattg aaacatccg aatattatt tgtttgttg 
ggagttgtg gtaaaacag gaacctatg tgtatgttg 
tttgaattc atgtcagaa ggtgatttg catgaattt 
ttgatctca aattcgcca agtgaaggt aaaagtttg 
acacagaac caattttta catatcgcc atacagatc 
gcacaagga atggaatat ctgtccgat aatcattat 
gttcatagg gatttggcg gcgaggaat tgtttagtt 
tccaaagat ttggttgtt aaaattagc gattttgga 
ctcagtaga gatatgtac agttgcgac tactaccgg 
gtccagtca aaatccctg ttgcccgtc cgctggatg 
ccccccgaa tccatcctc tacggcaaa ttcacgacc 
gaaagcgac gtctggtcg tacggcgtc gtcctctgg 
gagatctac agctacggc ctccagccc tactacggc 
tacaacaac caggaggtg atcaacatg atccgctcc 
cgcaaactc cttccatgc cccgacgct tgccccagc 
tactgttac gcgctgatg gtcgaatgc tgggccgaa 
cttgccgtc cgacggccc agcttcggc gaaatcagc 
caccgatta cgcgtttgg aagcaaaac ggcaacttg 
aacggtgcc tatttcaaa acgacggac agccacaaa 
ggcggccac agctccaag tcggaggag gacagcgcc 
acctgggag cgcaaacgg ctccacgag agtcagagc 
agtctcacg agtcgctcc tcgagtctt ggcaacaaa 
acgcaaagc acgagcttg agcaacgag cagtcgagg 
aatcggcgc agtcacagg aggaaggag gattcgctg 
gaccgggcg atgagcccc aaaaacacg attgttaat 
tcaaacggg gataatttc gagataagg attacacat 
tga
    mRNA sequence
    • >TC015667 RA
      gtggcgttg ccaacattt ttttggtgc gtttctatt 
tttagtaac ttttcatct gtcaaacca tcccaaaac 
attctttgt gtatattta cacaaatcg gcgttattt 
ccagtgcaa tttatttgt ttatcaacc gtgcatcga 
ccctttgcc cccttgacc cagacccgg tgccaaaca 
atgtcgctg acctaatca tgaacaaac acctctgtg 
tcttcctgc tcgcaacat cgacgattc ttgtttttg 
gcaacgaca atgactacg acgactatg aagaggacc 
taacccccc acaacgcct cctcaaact ccacccttg 
tcttcatcc gcccccttg ccaacctat caatcgaca 
ccgccacca aaaccattc gtctaacat gcgaagtac 
aaaacctgg acctaaaca acacaaacc gtgtcacca 
tcacgtggc gcaaaaact acgtcccga tcgaacgta 
gcgatcgca taattattc gaaaatcaa gcaaagaca 
acaaacatc gttccacac tacgtatca acgaaattg 
agttcttcg atcgtggtt tctacgatt gtacggcgt 
cgaatggcg tcgataaaa tccgatcgc aggcgcttg 
tggacgtcg cgacggact cgggacgcg tcgacagca 
ccgtggcgc tgaaattcg acggcgaaa tcacaacaa 
taccggcag ggactgttt ttgacgcca agttgccgg 
aactgccac acgaaaaaa tcaccaaca cttttcaaa 
tgcaatcgc aacggcctt ataatctca gtgagccgt 
tctgtcaag tgtatctag gggagacgt gcaaagact 
acttggata atcagtatg tttttgtgc agcccccgt 
atagtcaaa aggacatcg aacaaatgc taagtgatg 
cttttttgg tcatttcgc agtcaaacg atatttcat 
cgagttgta ataaattcg cacgtccaa gtttgtgtt 
attcggcgt ttcctttgt gtaccgatc cgaaacaaa 
taaatatgt atcagacga cgaaaatgg atcggttta 
agttagata atgtcggtt ttaagaatt tgaggaata 
aattgggga gttcgttga ggaggattt gtaaagagg 
attgtcttt tgttagaga ctgagttgt gtagtaagg 
aatatgcga ttgcgaaac gacatccag ttattggaa 
agactttgg aattggagg agtgtcagt ttttgccga 
atgagaccg aagtcagtt cgagaaatt gtctcgagt 
tgggtgtcg atcatgaaa gtgttaata aagaggata 
cgtgttatt gggacaacg gcgaactat acaggggtg 
ttcaaaata tgagcgaac ttggtaaca aatgtttaa 
ggtgggccc accaattcc acatccatt tatccgaac 
acccggaac tgacaggtc ataattatt gtcgtaatc 
caggtggcg ttgaatcgc agccatttt gttacgtcg 
atggtgacc cgattcggc gtgaaactt gtgacgtca 
ctaagtgca cctacgttg tttggatgt atgtcattg 
gtattttca tagccatct ctgttttta gtgtggttt 
tatttgggg ttttttgtt gccgacgaa gaaacaaac 
ttgcgcgca acttacaaa atatgaatg ttcctgtcg 
ctgataaga atatttatg gtagtggga gtcccacag 
ccccgccca tggaactaa acagtctcc tcccaccaa 
atattcctc cacaacgca actcccatt cgaccaaaa 
acggttatc agaacgtcc cccaatata cttacaaag 
aagttgctt ttgtggagg agttgggcg agggtgcgt 
tcggtaaag tgtacaaag gtgaattga aaacaaaaa 
atgggaaaa ttttcgttg ccgttaagt cattgaaag 
aaaatgcga gtgccaaaa ctcaggctg attttcagc 
gcgagattg aactgattt cggaattga aacatccga 
atattattt gtttgttgg gagttgtgg taaaacagg 
aacctatgt gtatgttgt ttgaattca tgtcagaag 
gtgatttgc atgaatttt tgatctcaa attcgccaa 
gtgaaggta aaagtttga cacagaacc aatttttac 
atatcgcca tacagatcg cacaaggaa tggaatatc 
tgtccgata atcattatg ttcataggg atttggcgg 
cgaggaatt gtttagttt ccaaagatt tggttgtta 
aaattagcg attttggac tcagtagag atatgtaca 
gttgcgact actaccggg tccagtcaa aatccctgt 
tgcccgtcc gctggatgc cccccgaat ccatcctct 
acggcaaat tcacgaccg aaagcgacg tctggtcgt 
acggcgtcg tcctctggg agatctaca gctacggcc 
tccagccct actacggct acaacaacc aggaggtga 
tcaacatga tccgctccc gcaaactcc ttccatgcc 
ccgacgctt gccccagct actgttacg cgctgatgg 
tcgaatgct gggccgaac ttgccgtcc gacggccca 
gcttcggcg aaatcagcc accgattac gcgtttgga 
agcaaaacg gcaacttga acggtgcct atttcaaaa 
cgacggaca gccacaaag gcggccaca gctccaagt 
cggaggagg acagcgcca cctgggagc gcaaacggc 
tccacgaga gtcagagca gtctcacga gtcgctcct 
cgagtcttg gcaacaaaa cgcaaagca cgagcttga 
gcaacgagc agtcgagga atcggcgca gtcacagga 
ggaaggagg attcgctgg accgggcga tgagcccca 
aaaacacga ttgttaatt caaacgggg ataatttcg 
agataagga ttacacatt gattgattg atttttttg 
ttcctggtc aatttatac caaattttt acgttgagt 
atttatccg ttttttatg tctttttct atggtgtgt 
cgtgtacgt cagtgtgtg tttataaaa tatatccaa 
gttttttta attaaatct ttattaaca attttagta 
tttacacag atgagacgg ccaactcca tttgaacaa 
ttaattacc catgctatc cctttcact ttttcccga 
ttgattgta tccttgttc agatggcgc cacggtgaa 
tttgttgat aagttagga aactgtaag tggttacgc 
gcttctttt ccgtcaact cattattgt ttcatacag 
gatgattgt aaatgaact actatcaag ctggctttg 
aacaagtgg tctaagcac acttttcaa gggagctac 
gtaagtact ttttatttt atttcgttt acacagaag 
cggtatttt tacaaacta ttcggtttt tgtactgac 
agtgatgtc agtgtgttg actatttaa aatatgata 
aaatttgtt ctgacaggt tgtcttaac ttttaataa 
ttctttttt gatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY