Status
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Gene information
General information
TC015667 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4498166..4524150
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015667
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05864

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    posterior terminus present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015667 RA
      MNKHLCVFL LATSTILVF GNDNDYDDY EEDLTPHNA 
      SSNSTLVFI RPLANLSID TATKTIRLT CEVQNLDLN 
      NTNRVTITW RKNYVPIER SDRIIIRKS SKDNKHRST 
      LRINEIEFF DRGFYDCTA SNGVDKIRS QALVDVATD 
      SGRVDSTVA LKFDGEITT IPAGTVFDA KLPELPHEK 
      ITNTFQMQS QRPYNLSEP FCQVYLGET CKDYLDNQY 
      VFVQPPYSQ KDIEQMLSD AFLVISQSN DISSSCNKF 
      ARPSLCYSA FPLCTDPKQ INMYQTTKM DRFKLDNVG 
      FKNLRNKLG SSLRRICKE DCLLLETEL CSKEYAIAK 
      RHPVIGKTL ELEECQFLP NETEVSSRN CLELGVDHE 
      SVNKEDTCY WDNGELYRG VQNMSELGN KCLRWAHQF 
      HIHLSEHPE LTGHNYCRN PGGVESQPF CYVDGDPIR 
      RETCDVTKC TYVVWMYVI GIFIAISVF SVVLFGVFC 
      CRRRNKLAR NLQNMNVPV ADKNIYGSG SPTAPPMEL 
      NSLLPPNIP PQRNSHSTK NGYQNVPQY TYKEVAFVE 
      ELGEGAFGK VYKGELKTK NGKIFVAVK SLKENASAK 
      TQADFQREI ELISELKHP NIICLLGVV VKQEPMCML 
      FEFMSEGDL HEFLISNSP SEGKSLTQN QFLHIAIQI 
      AQGMEYLSD NHYVHRDLA ARNCLVSKD LVVKISDFG 
      LSRDMYSCD YYRVQSKSL LPVRWMPPE SILYGKFTT 
      ESDVWSYGV VLWEIYSYG LQPYYGYNN QEVINMIRS 
      RKLLPCPDA CPSYCYALM VECWAELAV RRPSFGEIS 
      HRLRVWKQN GNLNGAYFK TTDSHKGGH SSKSEEDSA 
      TWERKRLHE SQSSLTSRS SSLGNKTQS TSLSNEQSR 
      NRRSHRRKE DSLDRAMSP KNTIVNSNG DNFEIRITH
    CDS sequence
    • >TC015667 RA
      atgaacaaa cacctctgt gtcttcctg ctcgcaaca 
      tcgacgatt cttgttttt ggcaacgac aatgactac 
      gacgactat gaagaggac ctaaccccc cacaacgcc 
      tcctcaaac tccaccctt gtcttcatc cgccccctt 
      gccaaccta tcaatcgac accgccacc aaaaccatt 
      cgtctaaca tgcgaagta caaaacctg gacctaaac 
      aacacaaac cgtgtcacc atcacgtgg cgcaaaaac 
      tacgtcccg atcgaacgt agcgatcgc ataattatt 
      cgaaaatca agcaaagac aacaaacat cgttccaca 
      ctacgtatc aacgaaatt gagttcttc gatcgtggt 
      ttctacgat tgtacggcg tcgaatggc gtcgataaa 
      atccgatcg caggcgctt gtggacgtc gcgacggac 
      tcgggacgc gtcgacagc accgtggcg ctgaaattc 
      gacggcgaa atcacaaca ataccggca gggactgtt 
      tttgacgcc aagttgccg gaactgcca cacgaaaaa 
      atcaccaac acttttcaa atgcaatcg caacggcct 
      tataatctc agtgagccg ttctgtcaa gtgtatcta 
      ggggagacg tgcaaagac tacttggat aatcagtat 
      gtttttgtg cagcccccg tatagtcaa aaggacatc 
      gaacaaatg ctaagtgat gcttttttg gtcatttcg 
      cagtcaaac gatatttca tcgagttgt aataaattc 
      gcacgtcca agtttgtgt tattcggcg tttcctttg 
      tgtaccgat ccgaaacaa ataaatatg tatcagacg 
      acgaaaatg gatcggttt aagttagat aatgtcggt 
      tttaagaat ttgaggaat aaattgggg agttcgttg 
      aggaggatt tgtaaagag gattgtctt ttgttagag 
      actgagttg tgtagtaag gaatatgcg attgcgaaa 
      cgacatcca gttattgga aagactttg gaattggag 
      gagtgtcag tttttgccg aatgagacc gaagtcagt 
      tcgagaaat tgtctcgag ttgggtgtc gatcatgaa 
      agtgttaat aaagaggat acgtgttat tgggacaac 
      ggcgaacta tacaggggt gttcaaaat atgagcgaa 
      cttggtaac aaatgttta aggtgggcc caccaattc 
      cacatccat ttatccgaa cacccggaa ctgacaggt 
      cataattat tgtcgtaat ccaggtggc gttgaatcg 
      cagccattt tgttacgtc gatggtgac ccgattcgg 
      cgtgaaact tgtgacgtc actaagtgc acctacgtt 
      gtttggatg tatgtcatt ggtattttc atagccatc 
      tctgttttt agtgtggtt ttatttggg gttttttgt 
      tgccgacga agaaacaaa cttgcgcgc aacttacaa 
      aatatgaat gttcctgtc gctgataag aatatttat 
      ggtagtggg agtcccaca gccccgccc atggaacta 
      aacagtctc ctcccacca aatattcct ccacaacgc 
      aactcccat tcgaccaaa aacggttat cagaacgtc 
      ccccaatat acttacaaa gaagttgct tttgtggag 
      gagttgggc gagggtgcg ttcggtaaa gtgtacaaa 
      ggtgaattg aaaacaaaa aatgggaaa attttcgtt 
      gccgttaag tcattgaaa gaaaatgcg agtgccaaa 
      actcaggct gattttcag cgcgagatt gaactgatt 
      tcggaattg aaacatccg aatattatt tgtttgttg 
      ggagttgtg gtaaaacag gaacctatg tgtatgttg 
      tttgaattc atgtcagaa ggtgatttg catgaattt 
      ttgatctca aattcgcca agtgaaggt aaaagtttg 
      acacagaac caattttta catatcgcc atacagatc 
      gcacaagga atggaatat ctgtccgat aatcattat 
      gttcatagg gatttggcg gcgaggaat tgtttagtt 
      tccaaagat ttggttgtt aaaattagc gattttgga 
      ctcagtaga gatatgtac agttgcgac tactaccgg 
      gtccagtca aaatccctg ttgcccgtc cgctggatg 
      ccccccgaa tccatcctc tacggcaaa ttcacgacc 
      gaaagcgac gtctggtcg tacggcgtc gtcctctgg 
      gagatctac agctacggc ctccagccc tactacggc 
      tacaacaac caggaggtg atcaacatg atccgctcc 
      cgcaaactc cttccatgc cccgacgct tgccccagc 
      tactgttac gcgctgatg gtcgaatgc tgggccgaa 
      cttgccgtc cgacggccc agcttcggc gaaatcagc 
      caccgatta cgcgtttgg aagcaaaac ggcaacttg 
      aacggtgcc tatttcaaa acgacggac agccacaaa 
      ggcggccac agctccaag tcggaggag gacagcgcc 
      acctgggag cgcaaacgg ctccacgag agtcagagc 
      agtctcacg agtcgctcc tcgagtctt ggcaacaaa 
      acgcaaagc acgagcttg agcaacgag cagtcgagg 
      aatcggcgc agtcacagg aggaaggag gattcgctg 
      gaccgggcg atgagcccc aaaaacacg attgttaat 
      tcaaacggg gataatttc gagataagg attacacat 
      tga
    mRNA sequence
    • >TC015667 RA
      gtggcgttg ccaacattt ttttggtgc gtttctatt 
      tttagtaac ttttcatct gtcaaacca tcccaaaac 
      attctttgt gtatattta cacaaatcg gcgttattt 
      ccagtgcaa tttatttgt ttatcaacc gtgcatcga 
      ccctttgcc cccttgacc cagacccgg tgccaaaca 
      atgtcgctg acctaatca tgaacaaac acctctgtg 
      tcttcctgc tcgcaacat cgacgattc ttgtttttg 
      gcaacgaca atgactacg acgactatg aagaggacc 
      taacccccc acaacgcct cctcaaact ccacccttg 
      tcttcatcc gcccccttg ccaacctat caatcgaca 
      ccgccacca aaaccattc gtctaacat gcgaagtac 
      aaaacctgg acctaaaca acacaaacc gtgtcacca 
      tcacgtggc gcaaaaact acgtcccga tcgaacgta 
      gcgatcgca taattattc gaaaatcaa gcaaagaca 
      acaaacatc gttccacac tacgtatca acgaaattg 
      agttcttcg atcgtggtt tctacgatt gtacggcgt 
      cgaatggcg tcgataaaa tccgatcgc aggcgcttg 
      tggacgtcg cgacggact cgggacgcg tcgacagca 
      ccgtggcgc tgaaattcg acggcgaaa tcacaacaa 
      taccggcag ggactgttt ttgacgcca agttgccgg 
      aactgccac acgaaaaaa tcaccaaca cttttcaaa 
      tgcaatcgc aacggcctt ataatctca gtgagccgt 
      tctgtcaag tgtatctag gggagacgt gcaaagact 
      acttggata atcagtatg tttttgtgc agcccccgt 
      atagtcaaa aggacatcg aacaaatgc taagtgatg 
      cttttttgg tcatttcgc agtcaaacg atatttcat 
      cgagttgta ataaattcg cacgtccaa gtttgtgtt 
      attcggcgt ttcctttgt gtaccgatc cgaaacaaa 
      taaatatgt atcagacga cgaaaatgg atcggttta 
      agttagata atgtcggtt ttaagaatt tgaggaata 
      aattgggga gttcgttga ggaggattt gtaaagagg 
      attgtcttt tgttagaga ctgagttgt gtagtaagg 
      aatatgcga ttgcgaaac gacatccag ttattggaa 
      agactttgg aattggagg agtgtcagt ttttgccga 
      atgagaccg aagtcagtt cgagaaatt gtctcgagt 
      tgggtgtcg atcatgaaa gtgttaata aagaggata 
      cgtgttatt gggacaacg gcgaactat acaggggtg 
      ttcaaaata tgagcgaac ttggtaaca aatgtttaa 
      ggtgggccc accaattcc acatccatt tatccgaac 
      acccggaac tgacaggtc ataattatt gtcgtaatc 
      caggtggcg ttgaatcgc agccatttt gttacgtcg 
      atggtgacc cgattcggc gtgaaactt gtgacgtca 
      ctaagtgca cctacgttg tttggatgt atgtcattg 
      gtattttca tagccatct ctgttttta gtgtggttt 
      tatttgggg ttttttgtt gccgacgaa gaaacaaac 
      ttgcgcgca acttacaaa atatgaatg ttcctgtcg 
      ctgataaga atatttatg gtagtggga gtcccacag 
      ccccgccca tggaactaa acagtctcc tcccaccaa 
      atattcctc cacaacgca actcccatt cgaccaaaa 
      acggttatc agaacgtcc cccaatata cttacaaag 
      aagttgctt ttgtggagg agttgggcg agggtgcgt 
      tcggtaaag tgtacaaag gtgaattga aaacaaaaa 
      atgggaaaa ttttcgttg ccgttaagt cattgaaag 
      aaaatgcga gtgccaaaa ctcaggctg attttcagc 
      gcgagattg aactgattt cggaattga aacatccga 
      atattattt gtttgttgg gagttgtgg taaaacagg 
      aacctatgt gtatgttgt ttgaattca tgtcagaag 
      gtgatttgc atgaatttt tgatctcaa attcgccaa 
      gtgaaggta aaagtttga cacagaacc aatttttac 
      atatcgcca tacagatcg cacaaggaa tggaatatc 
      tgtccgata atcattatg ttcataggg atttggcgg 
      cgaggaatt gtttagttt ccaaagatt tggttgtta 
      aaattagcg attttggac tcagtagag atatgtaca 
      gttgcgact actaccggg tccagtcaa aatccctgt 
      tgcccgtcc gctggatgc cccccgaat ccatcctct 
      acggcaaat tcacgaccg aaagcgacg tctggtcgt 
      acggcgtcg tcctctggg agatctaca gctacggcc 
      tccagccct actacggct acaacaacc aggaggtga 
      tcaacatga tccgctccc gcaaactcc ttccatgcc 
      ccgacgctt gccccagct actgttacg cgctgatgg 
      tcgaatgct gggccgaac ttgccgtcc gacggccca 
      gcttcggcg aaatcagcc accgattac gcgtttgga 
      agcaaaacg gcaacttga acggtgcct atttcaaaa 
      cgacggaca gccacaaag gcggccaca gctccaagt 
      cggaggagg acagcgcca cctgggagc gcaaacggc 
      tccacgaga gtcagagca gtctcacga gtcgctcct 
      cgagtcttg gcaacaaaa cgcaaagca cgagcttga 
      gcaacgagc agtcgagga atcggcgca gtcacagga 
      ggaaggagg attcgctgg accgggcga tgagcccca 
      aaaacacga ttgttaatt caaacgggg ataatttcg 
      agataagga ttacacatt gattgattg atttttttg 
      ttcctggtc aatttatac caaattttt acgttgagt 
      atttatccg ttttttatg tctttttct atggtgtgt 
      cgtgtacgt cagtgtgtg tttataaaa tatatccaa 
      gttttttta attaaatct ttattaaca attttagta 
      tttacacag atgagacgg ccaactcca tttgaacaa 
      ttaattacc catgctatc cctttcact ttttcccga 
      ttgattgta tccttgttc agatggcgc cacggtgaa 
      tttgttgat aagttagga aactgtaag tggttacgc 
      gcttctttt ccgtcaact cattattgt ttcatacag 
      gatgattgt aaatgaact actatcaag ctggctttg 
      aacaagtgg tctaagcac acttttcaa gggagctac 
      gtaagtact ttttatttt atttcgttt acacagaag 
      cggtatttt tacaaacta ttcggtttt tgtactgac 
      agtgatgtc agtgtgttg actatttaa aatatgata 
      aaatttgtt ctgacaggt tgtcttaac ttttaataa 
      ttctttttt gatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY