General information | |||
TC015667 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 4498166..4524150 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC015667 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05864 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
antenna not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
posterior terminus present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC015667 RA
MNKHLCVFL LATSTILVF GNDNDYDDY EEDLTPHNA SSNSTLVFI RPLANLSID TATKTIRLT CEVQNLDLN NTNRVTITW RKNYVPIER SDRIIIRKS SKDNKHRST LRINEIEFF DRGFYDCTA SNGVDKIRS QALVDVATD SGRVDSTVA LKFDGEITT IPAGTVFDA KLPELPHEK ITNTFQMQS QRPYNLSEP FCQVYLGET CKDYLDNQY VFVQPPYSQ KDIEQMLSD AFLVISQSN DISSSCNKF ARPSLCYSA FPLCTDPKQ INMYQTTKM DRFKLDNVG FKNLRNKLG SSLRRICKE DCLLLETEL CSKEYAIAK RHPVIGKTL ELEECQFLP NETEVSSRN CLELGVDHE SVNKEDTCY WDNGELYRG VQNMSELGN KCLRWAHQF HIHLSEHPE LTGHNYCRN PGGVESQPF CYVDGDPIR RETCDVTKC TYVVWMYVI GIFIAISVF SVVLFGVFC CRRRNKLAR NLQNMNVPV ADKNIYGSG SPTAPPMEL NSLLPPNIP PQRNSHSTK NGYQNVPQY TYKEVAFVE ELGEGAFGK VYKGELKTK NGKIFVAVK SLKENASAK TQADFQREI ELISELKHP NIICLLGVV VKQEPMCML FEFMSEGDL HEFLISNSP SEGKSLTQN QFLHIAIQI AQGMEYLSD NHYVHRDLA ARNCLVSKD LVVKISDFG LSRDMYSCD YYRVQSKSL LPVRWMPPE SILYGKFTT ESDVWSYGV VLWEIYSYG LQPYYGYNN QEVINMIRS RKLLPCPDA CPSYCYALM VECWAELAV RRPSFGEIS HRLRVWKQN GNLNGAYFK TTDSHKGGH SSKSEEDSA TWERKRLHE SQSSLTSRS SSLGNKTQS TSLSNEQSR NRRSHRRKE DSLDRAMSP KNTIVNSNG DNFEIRITH
- >TC015667 RA
atgaacaaa cacctctgt gtcttcctg ctcgcaaca tcgacgatt cttgttttt ggcaacgac aatgactac gacgactat gaagaggac ctaaccccc cacaacgcc tcctcaaac tccaccctt gtcttcatc cgccccctt gccaaccta tcaatcgac accgccacc aaaaccatt cgtctaaca tgcgaagta caaaacctg gacctaaac aacacaaac cgtgtcacc atcacgtgg cgcaaaaac tacgtcccg atcgaacgt agcgatcgc ataattatt cgaaaatca agcaaagac aacaaacat cgttccaca ctacgtatc aacgaaatt gagttcttc gatcgtggt ttctacgat tgtacggcg tcgaatggc gtcgataaa atccgatcg caggcgctt gtggacgtc gcgacggac tcgggacgc gtcgacagc accgtggcg ctgaaattc gacggcgaa atcacaaca ataccggca gggactgtt tttgacgcc aagttgccg gaactgcca cacgaaaaa atcaccaac acttttcaa atgcaatcg caacggcct tataatctc agtgagccg ttctgtcaa gtgtatcta ggggagacg tgcaaagac tacttggat aatcagtat gtttttgtg cagcccccg tatagtcaa aaggacatc gaacaaatg ctaagtgat gcttttttg gtcatttcg cagtcaaac gatatttca tcgagttgt aataaattc gcacgtcca agtttgtgt tattcggcg tttcctttg tgtaccgat ccgaaacaa ataaatatg tatcagacg acgaaaatg gatcggttt aagttagat aatgtcggt tttaagaat ttgaggaat aaattgggg agttcgttg aggaggatt tgtaaagag gattgtctt ttgttagag actgagttg tgtagtaag gaatatgcg attgcgaaa cgacatcca gttattgga aagactttg gaattggag gagtgtcag tttttgccg aatgagacc gaagtcagt tcgagaaat tgtctcgag ttgggtgtc gatcatgaa agtgttaat aaagaggat acgtgttat tgggacaac ggcgaacta tacaggggt gttcaaaat atgagcgaa cttggtaac aaatgttta aggtgggcc caccaattc cacatccat ttatccgaa cacccggaa ctgacaggt cataattat tgtcgtaat ccaggtggc gttgaatcg cagccattt tgttacgtc gatggtgac ccgattcgg cgtgaaact tgtgacgtc actaagtgc acctacgtt gtttggatg tatgtcatt ggtattttc atagccatc tctgttttt agtgtggtt ttatttggg gttttttgt tgccgacga agaaacaaa cttgcgcgc aacttacaa aatatgaat gttcctgtc gctgataag aatatttat ggtagtggg agtcccaca gccccgccc atggaacta aacagtctc ctcccacca aatattcct ccacaacgc aactcccat tcgaccaaa aacggttat cagaacgtc ccccaatat acttacaaa gaagttgct tttgtggag gagttgggc gagggtgcg ttcggtaaa gtgtacaaa ggtgaattg aaaacaaaa aatgggaaa attttcgtt gccgttaag tcattgaaa gaaaatgcg agtgccaaa actcaggct gattttcag cgcgagatt gaactgatt tcggaattg aaacatccg aatattatt tgtttgttg ggagttgtg gtaaaacag gaacctatg tgtatgttg tttgaattc atgtcagaa ggtgatttg catgaattt ttgatctca aattcgcca agtgaaggt aaaagtttg acacagaac caattttta catatcgcc atacagatc gcacaagga atggaatat ctgtccgat aatcattat gttcatagg gatttggcg gcgaggaat tgtttagtt tccaaagat ttggttgtt aaaattagc gattttgga ctcagtaga gatatgtac agttgcgac tactaccgg gtccagtca aaatccctg ttgcccgtc cgctggatg ccccccgaa tccatcctc tacggcaaa ttcacgacc gaaagcgac gtctggtcg tacggcgtc gtcctctgg gagatctac agctacggc ctccagccc tactacggc tacaacaac caggaggtg atcaacatg atccgctcc cgcaaactc cttccatgc cccgacgct tgccccagc tactgttac gcgctgatg gtcgaatgc tgggccgaa cttgccgtc cgacggccc agcttcggc gaaatcagc caccgatta cgcgtttgg aagcaaaac ggcaacttg aacggtgcc tatttcaaa acgacggac agccacaaa ggcggccac agctccaag tcggaggag gacagcgcc acctgggag cgcaaacgg ctccacgag agtcagagc agtctcacg agtcgctcc tcgagtctt ggcaacaaa acgcaaagc acgagcttg agcaacgag cagtcgagg aatcggcgc agtcacagg aggaaggag gattcgctg gaccgggcg atgagcccc aaaaacacg attgttaat tcaaacggg gataatttc gagataagg attacacat tga
- >TC015667 RA
gtggcgttg ccaacattt ttttggtgc gtttctatt tttagtaac ttttcatct gtcaaacca tcccaaaac attctttgt gtatattta cacaaatcg gcgttattt ccagtgcaa tttatttgt ttatcaacc gtgcatcga ccctttgcc cccttgacc cagacccgg tgccaaaca atgtcgctg acctaatca tgaacaaac acctctgtg tcttcctgc tcgcaacat cgacgattc ttgtttttg gcaacgaca atgactacg acgactatg aagaggacc taacccccc acaacgcct cctcaaact ccacccttg tcttcatcc gcccccttg ccaacctat caatcgaca ccgccacca aaaccattc gtctaacat gcgaagtac aaaacctgg acctaaaca acacaaacc gtgtcacca tcacgtggc gcaaaaact acgtcccga tcgaacgta gcgatcgca taattattc gaaaatcaa gcaaagaca acaaacatc gttccacac tacgtatca acgaaattg agttcttcg atcgtggtt tctacgatt gtacggcgt cgaatggcg tcgataaaa tccgatcgc aggcgcttg tggacgtcg cgacggact cgggacgcg tcgacagca ccgtggcgc tgaaattcg acggcgaaa tcacaacaa taccggcag ggactgttt ttgacgcca agttgccgg aactgccac acgaaaaaa tcaccaaca cttttcaaa tgcaatcgc aacggcctt ataatctca gtgagccgt tctgtcaag tgtatctag gggagacgt gcaaagact acttggata atcagtatg tttttgtgc agcccccgt atagtcaaa aggacatcg aacaaatgc taagtgatg cttttttgg tcatttcgc agtcaaacg atatttcat cgagttgta ataaattcg cacgtccaa gtttgtgtt attcggcgt ttcctttgt gtaccgatc cgaaacaaa taaatatgt atcagacga cgaaaatgg atcggttta agttagata atgtcggtt ttaagaatt tgaggaata aattgggga gttcgttga ggaggattt gtaaagagg attgtcttt tgttagaga ctgagttgt gtagtaagg aatatgcga ttgcgaaac gacatccag ttattggaa agactttgg aattggagg agtgtcagt ttttgccga atgagaccg aagtcagtt cgagaaatt gtctcgagt tgggtgtcg atcatgaaa gtgttaata aagaggata cgtgttatt gggacaacg gcgaactat acaggggtg ttcaaaata tgagcgaac ttggtaaca aatgtttaa ggtgggccc accaattcc acatccatt tatccgaac acccggaac tgacaggtc ataattatt gtcgtaatc caggtggcg ttgaatcgc agccatttt gttacgtcg atggtgacc cgattcggc gtgaaactt gtgacgtca ctaagtgca cctacgttg tttggatgt atgtcattg gtattttca tagccatct ctgttttta gtgtggttt tatttgggg ttttttgtt gccgacgaa gaaacaaac ttgcgcgca acttacaaa atatgaatg ttcctgtcg ctgataaga atatttatg gtagtggga gtcccacag ccccgccca tggaactaa acagtctcc tcccaccaa atattcctc cacaacgca actcccatt cgaccaaaa acggttatc agaacgtcc cccaatata cttacaaag aagttgctt ttgtggagg agttgggcg agggtgcgt tcggtaaag tgtacaaag gtgaattga aaacaaaaa atgggaaaa ttttcgttg ccgttaagt cattgaaag aaaatgcga gtgccaaaa ctcaggctg attttcagc gcgagattg aactgattt cggaattga aacatccga atattattt gtttgttgg gagttgtgg taaaacagg aacctatgt gtatgttgt ttgaattca tgtcagaag gtgatttgc atgaatttt tgatctcaa attcgccaa gtgaaggta aaagtttga cacagaacc aatttttac atatcgcca tacagatcg cacaaggaa tggaatatc tgtccgata atcattatg ttcataggg atttggcgg cgaggaatt gtttagttt ccaaagatt tggttgtta aaattagcg attttggac tcagtagag atatgtaca gttgcgact actaccggg tccagtcaa aatccctgt tgcccgtcc gctggatgc cccccgaat ccatcctct acggcaaat tcacgaccg aaagcgacg tctggtcgt acggcgtcg tcctctggg agatctaca gctacggcc tccagccct actacggct acaacaacc aggaggtga tcaacatga tccgctccc gcaaactcc ttccatgcc ccgacgctt gccccagct actgttacg cgctgatgg tcgaatgct gggccgaac ttgccgtcc gacggccca gcttcggcg aaatcagcc accgattac gcgtttgga agcaaaacg gcaacttga acggtgcct atttcaaaa cgacggaca gccacaaag gcggccaca gctccaagt cggaggagg acagcgcca cctgggagc gcaaacggc tccacgaga gtcagagca gtctcacga gtcgctcct cgagtcttg gcaacaaaa cgcaaagca cgagcttga gcaacgagc agtcgagga atcggcgca gtcacagga ggaaggagg attcgctgg accgggcga tgagcccca aaaacacga ttgttaatt caaacgggg ataatttcg agataagga ttacacatt gattgattg atttttttg ttcctggtc aatttatac caaattttt acgttgagt atttatccg ttttttatg tctttttct atggtgtgt cgtgtacgt cagtgtgtg tttataaaa tatatccaa gttttttta attaaatct ttattaaca attttagta tttacacag atgagacgg ccaactcca tttgaacaa ttaattacc catgctatc cctttcact ttttcccga ttgattgta tccttgttc agatggcgc cacggtgaa tttgttgat aagttagga aactgtaag tggttacgc gcttctttt ccgtcaact cattattgt ttcatacag gatgattgt aaatgaact actatcaag ctggctttg aacaagtgg tctaagcac acttttcaa gggagctac gtaagtact ttttatttt atttcgttt acacagaag cggtatttt tacaaacta ttcggtttt tgtactgac agtgatgtc agtgtgttg actatttaa aatatgata aaatttgtt ctgacaggt tgtcttaac ttttaataa ttctttttt gatttt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
