Status
Please wait ...
Gene information
General information
TC015658 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4291356..4292502
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015658
    iBeetle screen iB_05862

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015658 RA
      MTTSVQKFL DVLTDSPQI SDVRMNRFL MKSPLQLIT 
      TLGLYLATV LLIGPRLMR NRKPFSLKG TIVAYNVIQ 
      ICWNLYLFL GFVGLSNNL SIVCTPYEK SNRQEVLQM 
      IELHYRYIL LKHFDLIET MFFVLRKKE RQVSFLHVY 
      HHVGILLAA WVSGKYFPG GQATFVGLY NTFVHLVMY 
      CYYLLSSYK SSETLWWKK YVTLLQIVQ HCLVFLCVF 
      PSVVNPNCS YPKFWMGLF SINVLFIIY LFGKFYKNT 
      YLDKKRQ
    CDS sequence
    • >TC015658 RA
      atgactacg tccgtgcaa aaattccta gatgtgtta 
      acggattcg ccacaaatt tcggatgta cgcatgaac 
      cgatttttg atgaaatca ccgcttcaa ctaatcaca 
      actttggga ctttatctc gccactgtt ctacttatc 
      ggcccgaga cttatgcga aatcggaaa cccttttca 
      ctaaaaggc acaatagtt gcgtacaat gtcatacaa 
      atttgttgg aacctttac ctattttta gggtttgta 
      ggactttcc aataattta agcatcgtt tgcacacct 
      tatgaaaaa tccaaccga caagaagtg ttacaaatg 
      atagaattg cactaccgg tacattctt ctaaaacac 
      ttcgattta attgaaact atgtttttc gttttgagg 
      aaaaaagag cgccaagtg tccttcttg cacgtttac 
      catcacgtt ggcatcctg ctggcggcc tgggtttcg 
      ggcaagtac ttccccgga ggacaggcc acctttgtg 
      gggctttac aacactttt gtgcacctt gttatgtac 
      tgttactat ttgttaagt tcgtacaaa tcgagtgaa 
      actctgtgg tggaaaaaa tacgtcacg ctgttgcaa 
      atcgtgcaa cactgtctc gtgtttttg tgcgttttt 
      ccctccgtg gtgaacccg aactgttcg tacccgaaa 
      ttttggatg ggtctgttc tcgatcaac gtcctcttc 
      attatttat ctgttcgga aagttttac aaaaacacg 
      tatttggac aaaaagagg caatag
    mRNA sequence
    • >TC015658 RA
      tatggtaaa ttccataag gtggctgac catgattgt 
      tagttttca caacacttc agtatttcc tctgctaga 
      aaagacaca acaaacaac atgactacg tccgtgcaa 
      aaattccta gatgtgtta acggattcg ccacaaatt 
      tcggatgta cgcatgaac cgatttttg atgaaatca 
      ccgcttcaa ctaatcaca actttggga ctttatctc 
      gccactgtt ctacttatc ggcccgaga cttatgcga 
      aatcggaaa cccttttca ctaaaaggc acaatagtt 
      gcgtacaat gtcatacaa atttgttgg aacctttac 
      ctattttta gggtttgta ggactttcc aataattta 
      agcatcgtt tgcacacct tatgaaaaa tccaaccga 
      caagaagtg ttacaaatg atagaattg cactaccgg 
      tacattctt ctaaaacac ttcgattta attgaaact 
      atgtttttc gttttgagg aaaaaagag cgccaagtg 
      tccttcttg cacgtttac catcacgtt ggcatcctg 
      ctggcggcc tgggtttcg ggcaagtac ttccccgga 
      ggacaggcc acctttgtg gggctttac aacactttt 
      gtgcacctt gttatgtac tgttactat ttgttaagt 
      tcgtacaaa tcgagtgaa actctgtgg tggaaaaaa 
      tacgtcacg ctgttgcaa atcgtgcaa cactgtctc 
      gtgtttttg tgcgttttt ccctccgtg gtgaacccg 
      aactgttcg tacccgaaa ttttggatg ggtctgttc 
      tcgatcaac gtcctcttc attatttat ctgttcgga 
      aagttttac aaaaacacg tatttggac aaaaagagg 
      caatagtgt ggttgagag tcgtgtgtg tttatattt 
      ttgtaaatt gtacaaatc tgggttaat aaattatta 
      tatggttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY