| General information | |||
| TC015652 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 4144714..4176412 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015652 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05861 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015652 RA
MIIMDSVVI PRPPALPPV PLAPPTPKP PSLTSTTSG FRLMEDQGD TGSVYSIYK QKIDAMFES DSSTNTKNS VQARIEKMF SDVAQDNGI ILSDGTTHR FSVDYLGSV GLTDKVTSL AGLQGPLKD LYFAYKRTT RAKTLTGKL EISGQGLKV QYQGEKGDL EQMNSFPTI AVWSAVKFV IHKTSYAFL PLITDPDNI DKKALFREL SDGEKKFIN GETHSPLFA VVMRKIGLQ KQLECHGFV CQTSEDAIV IAATLYKSL MAHMKAKEK RPKNRNGIT CISTTSSTF NEKSVPVRP PRKKRSTTS SIISENEQL DVSDTQPLI QKKNVRKSI KSRPAPKTP DFDAILPYE EPVKPEEIK PKTFSDQIN TFMSREQKQ ITAEIKQMM NEKVIKPAE SGEGNSLRK KDNSGDILT KVTIPRSGS FLNAGGLTR YKSKVSRSN GQATGGSPL GFNELFNEF RLQEGLHSM DEILGVIID PEGMSFNDL KPIYKEFLL KLSVTLTKD ELYQRSKSI MRRQKKKLL RRNSLQTKP THHILVGSK FKRLKHIFR KNLKLKLGR NKSESDPGP GKSPESSIS TSSYDTRQF SPRHEPKPK KRDRVSTSE ESDFFSLRR RKMAGNHNH HQNRNSSSG YVSCSECSY DSDTCTCVS ADKCYCSLG NKQNARKNQ CDCNLDGPV TFCGCDTDS CTESNKCYC QNPARSRTI QELKRRSLK KDRKLCRRA SNSRSTKSL EYIGPYYEK LGSKRKNYN GVAVDYELF SVRSGRGRN DRTLQSTAI SGACTEALS VKKSAEIAA LFADIKLSQ TTDIAHLTP RTCDMDTII SSRNAYNKL KSQHEAKKI IQNGLYVRN NPKMYSARN GLYTIQSQS DESRRSSLS EEKNYFDLT KKNSSNLEN SLGYLP
- >TC015652 RA
atgataatc atggacagc gtcgtcatc ccccgtccc cccgccctc cctccagtc cccctcgcc cccccaacc cccaaaccc cccagtcta acgagcaca acatccggt tttcgccta atggaggac caaggcgac accggaagc gtctattcc atctacaag caaaaaatc gacgcaatg ttcgagtcc gattcgagc acgaacaca aaaaacagc gtccaagcg cgcatcgag aaaatgttc agcgacgtc gcccaagac aatggtatt attctgagc gatgggaca acccatcgc ttctctgtg gactacctg ggctcggtc ggtctaacc gacaaagtc acaagtctc gcaggcctc caagggccg ctcaaagac ctctatttc gcctacaaa aggacgaca agggccaag acactcacc gggaagttg gagatttcc ggccaaggg ctcaaagtg caataccaa ggcgaaaag ggcgacttg gagcaaatg aattcgttt ccgaccatt gctgtgtgg tcggcggtc aagtttgtg atacataag acgagttat gcttttttg ccactgatt accgatcct gataatatt gataagaaa gcattgttt cgggagttg agcgatggg gagaagaag tttatcaat ggggagacg cattcgcca ctttttgca gtcgtgatg agaaaaatc gggcttcag aagcaacta gagtgccac ggtttcgtc tgtcaaaca tccgaagac gcgatcgtc atcgctgca actctctac aagtcatta atggcccac atgaaagca aaagagaaa cggccgaaa aaccgcaac ggaatcact tgtatttca acaacttcg agcactttt aacgaaaaa tcggtccca gtccgacca ccgcgcaaa aaacgaagc acgacttcg tccataatt agcgaaaat gaacagctt gatgtgtct gatactcaa cccctgatt cagaaaaag aacgtccgg aagagtata aagtcgcgg ccggcgccc aaaaccccg gactttgat gcaattttg ccgtatgaa gagccggtt aaaccggag gaaatcaaa ccgaagacg ttttccgat cagatcaat acgtttatg agtcgggaa cagaaacaa atcacggcg gaaattaag caaatgatg aacgagaaa gtgatcaag ccggcggaa agtggcgaa gggaatagt ttgaggaag aaggataat tcgggtgat atcctgacg aaggtgacg atacccagg tcggggagt tttctcaat gctgggggt ctgacgcgg tataaatcg aaagtttca agaagcaac ggccaagca accggcggt tcccctctc ggcttcaac gaactcttc aacgaattc cgcctccag gagggcctc cactccatg gacgaaatc ctcggcgta ataatcgac cccgaagga atgtccttc aacgacctc aaacccatc tacaaggag ttcctcctc aagctctcc gtcaccctc acaaaagac gaactctac cagcgctcc aaatcgata atgcgccgc caaaaaaag aaactcctc cgccgaaac agcctccaa acaaagccc acccaccac atcctcgtc ggcagcaaa ttcaagcgt ctcaagcac atcttccgc aaaaacctc aaactcaag ctgggccgc aacaagtcc gagtccgac cccggcccc gggaaatcc cccgagagc agcatctcg acgtcgagc tacgacact cgccagttt tcgccgcgc cacgagccc aagccgaag aagcgcgac cgcgtctcc acctccgaa gagagcgac tttttcagc ctgcggcgc aggaaaatg gcggggaat cacaaccac caccagaat cggaactct tcgagcggc tacgtctcg tgctcggaa tgctcctac gacagcgac acgtgcact tgcgtctcg gccgataag tgctactgc tcgctggga aataaacaa aacgcgagg aaaaatcaa tgtgattgc aatctggac ggtccggtg acgttctgc gggtgtgat accgactca tgcaccgaa agcaataaa tgttactgt cagaacccg gccaggagt cggacgatt caggagttg aagcggcgc agtttgaag aaagacagg aagttgtgc aggagggcg tcgaattcg aggagtacg aagagtttg gagtacatt gggccgtat tatgagaag ttgggctcg aaaaggaag aattataac ggggtggcg gtggattat gagctgttc agtgtgagg agcgggaga gggaggaat gaccggaca ttgcaatcg actgccatt tccggcgcc tgcaccgaa gccctctct gtgaaaaaa tccgccgaa attgccgcc cttttcgcc gacattaaa ctaagtcaa acgacagac atcgcccac ttgacgccc cgaacttgc gacatggac acgattatt tcctcacga aatgcgtac aataaactg aaatcgcaa cacgaggcc aaaaaaatc atacagaac ggcctttat gtgagaaat aatccgaaa atgtacagt gcgaggaac ggactctac actattcag agccaatcg gatgaatcc agacgctcg agtttgagt gaggagaag aattatttt gatttgacg aagaaaaat agcagtaat ttggagaat tccttgggg tatttgccc taa
- >TC015652 RA
catttcaaa aaccccaac taaattgct tcactcact cgctcaaat tgccaaaac ccaatcaat caaacttgc ggtcggcac ccattatca gtcccaacc gcaacagac ccatctcca accatccca aatcaaatc tcatcgaaa gtgaccaaa tcccaacca tttaaaaaa ccacaccag caaacacaa ccgttttcc tccacagca ccgtgactg aaattcact cactccacg ccaaaagtc agtcacttg caagaatcg gcgctgagt gctcccgtg ctataccga gtgagttga acgcgcgat gagacgctg agctgtgct tttgtgaaa ttatgtgac cctttgggc taccagatg cgggattat ctcaaagct gccgatacc cacccacga tgataatca tggacagcg tcgtcatcc cccgtcccc ccgccctcc ctccagtcc ccctcgccc ccccaaccc ccaaacccc ccagtctaa cgagcacaa catccggtt ttcgcctaa tggaggacc aaggcgaca ccggaagcg tctattcca tctacaagc aaaaaatcg acgcaatgt tcgagtccg attcgagca cgaacacaa aaaacagcg tccaagcgc gcatcgaga aaatgttca gcgacgtcg cccaagaca atggtatta ttctgagcg atgggacaa cccatcgct tctctgtgg actacctgg gctcggtcg gtctaaccg acaaagtca caagtctcg caggcctcc aagggccgc tcaaagacc tctatttcg cctacaaaa ggacgacaa gggccaaga cactcaccg ggaagttgg agatttccg gccaagggc tcaaagtgc aataccaag gcgaaaagg gcgacttgg agcaaatga attcgtttc cgaccattg ctgtgtggt cggcggtca agtttgtga tacataaga cgagttatg cttttttgc cactgatta ccgatcctg ataatattg ataagaaag cattgtttc gggagttga gcgatgggg agaagaagt ttatcaatg gggagacgc attcgccac tttttgcag tcgtgatga gaaaaatcg ggcttcaga agcaactag agtgccacg gtttcgtct gtcaaacat ccgaagacg cgatcgtca tcgctgcaa ctctctaca agtcattaa tggcccaca tgaaagcaa aagagaaac ggccgaaaa accgcaacg gaatcactt gtatttcaa caacttcga gcactttta acgaaaaat cggtcccag tccgaccac cgcgcaaaa aacgaagca cgacttcgt ccataatta gcgaaaatg aacagcttg atgtgtctg atactcaac ccctgattc agaaaaaga acgtccgga agagtataa agtcgcggc cggcgccca aaaccccgg actttgatg caattttgc cgtatgaag agccggtta aaccggagg aaatcaaac cgaagacgt tttccgatc agatcaata cgtttatga gtcgggaac agaaacaaa tcacggcgg aaattaagc aaatgatga acgagaaag tgatcaagc cggcggaaa gtggcgaag ggaatagtt tgaggaaga aggataatt cgggtgata tcctgacga aggtgacga tacccaggt cggggagtt ttctcaatg ctgggggtc tgacgcggt ataaatcga aagtttcaa gaagcaacg gccaagcaa ccggcggtt cccctctcg gcttcaacg aactcttca acgaattcc gcctccagg agggcctcc actccatgg acgaaatcc tcggcgtaa taatcgacc ccgaaggaa tgtccttca acgacctca aacccatct acaaggagt tcctcctca agctctccg tcaccctca caaaagacg aactctacc agcgctcca aatcgataa tgcgccgcc aaaaaaaga aactcctcc gccgaaaca gcctccaaa caaagccca cccaccaca tcctcgtcg gcagcaaat tcaagcgtc tcaagcaca tcttccgca aaaacctca aactcaagc tgggccgca acaagtccg agtccgacc ccggccccg ggaaatccc ccgagagca gcatctcga cgtcgagct acgacactc gccagtttt cgccgcgcc acgagccca agccgaaga agcgcgacc gcgtctcca cctccgaag agagcgact ttttcagcc tgcggcgca ggaaaatgg cggggaatc acaaccacc accagaatc ggaactctt cgagcggct acgtctcgt gctcggaat gctcctacg acagcgaca cgtgcactt gcgtctcgg ccgataagt gctactgct cgctgggaa ataaacaaa acgcgagga aaaatcaat gtgattgca atctggacg gtccggtga cgttctgcg ggtgtgata ccgactcat gcaccgaaa gcaataaat gttactgtc agaacccgg ccaggagtc ggacgattc aggagttga agcggcgca gtttgaaga aagacagga agttgtgca ggagggcgt cgaattcga ggagtacga agagtttgg agtacattg ggccgtatt atgagaagt tgggctcga aaaggaaga attataacg gggtggcgg tggattatg agctgttca gtgtgagga gcgggagag ggaggaatg accggacat tgcaatcga ctgccattt ccggcgcct gcaccgaag ccctctctg tgaaaaaat ccgccgaaa ttgccgccc ttttcgccg acattaaac taagtcaaa cgacagaca tcgcccact tgacgcccc gaacttgcg acatggaca cgattattt cctcacgaa atgcgtaca ataaactga aatcgcaac acgaggcca aaaaaatca tacagaacg gcctttatg tgagaaata atccgaaaa tgtacagtg cgaggaacg gactctaca ctattcaga gccaatcgg atgaatcca gacgctcga gtttgagtg aggagaaga attattttg atttgacga agaaaaata gcagtaatt tggagaatt ccttggggt atttgccct aaatgatgt gtacagtga gggataaaa tgccatttc tttcgtttt ggggggttt gagttgagg tagggttgt tagagggga gaaatttta attaagtgc aataatatg tgcaatatt gttatttat gtgcaataa tttatcgtt aaggaataa agattttat ctcaga
