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Gene information
General information
TC015652 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4144714..4176412
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015652
    iBeetle screen iB_05861

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015652 RA
      MIIMDSVVI PRPPALPPV PLAPPTPKP PSLTSTTSG 
FRLMEDQGD TGSVYSIYK QKIDAMFES DSSTNTKNS 
VQARIEKMF SDVAQDNGI ILSDGTTHR FSVDYLGSV 
GLTDKVTSL AGLQGPLKD LYFAYKRTT RAKTLTGKL 
EISGQGLKV QYQGEKGDL EQMNSFPTI AVWSAVKFV 
IHKTSYAFL PLITDPDNI DKKALFREL SDGEKKFIN 
GETHSPLFA VVMRKIGLQ KQLECHGFV CQTSEDAIV 
IAATLYKSL MAHMKAKEK RPKNRNGIT CISTTSSTF 
NEKSVPVRP PRKKRSTTS SIISENEQL DVSDTQPLI 
QKKNVRKSI KSRPAPKTP DFDAILPYE EPVKPEEIK 
PKTFSDQIN TFMSREQKQ ITAEIKQMM NEKVIKPAE 
SGEGNSLRK KDNSGDILT KVTIPRSGS FLNAGGLTR 
YKSKVSRSN GQATGGSPL GFNELFNEF RLQEGLHSM 
DEILGVIID PEGMSFNDL KPIYKEFLL KLSVTLTKD 
ELYQRSKSI MRRQKKKLL RRNSLQTKP THHILVGSK 
FKRLKHIFR KNLKLKLGR NKSESDPGP GKSPESSIS 
TSSYDTRQF SPRHEPKPK KRDRVSTSE ESDFFSLRR 
RKMAGNHNH HQNRNSSSG YVSCSECSY DSDTCTCVS 
ADKCYCSLG NKQNARKNQ CDCNLDGPV TFCGCDTDS 
CTESNKCYC QNPARSRTI QELKRRSLK KDRKLCRRA 
SNSRSTKSL EYIGPYYEK LGSKRKNYN GVAVDYELF 
SVRSGRGRN DRTLQSTAI SGACTEALS VKKSAEIAA 
LFADIKLSQ TTDIAHLTP RTCDMDTII SSRNAYNKL 
KSQHEAKKI IQNGLYVRN NPKMYSARN GLYTIQSQS 
DESRRSSLS EEKNYFDLT KKNSSNLEN SLGYLP
    CDS sequence
    • >TC015652 RA
      atgataatc atggacagc gtcgtcatc ccccgtccc 
cccgccctc cctccagtc cccctcgcc cccccaacc 
cccaaaccc cccagtcta acgagcaca acatccggt 
tttcgccta atggaggac caaggcgac accggaagc 
gtctattcc atctacaag caaaaaatc gacgcaatg 
ttcgagtcc gattcgagc acgaacaca aaaaacagc 
gtccaagcg cgcatcgag aaaatgttc agcgacgtc 
gcccaagac aatggtatt attctgagc gatgggaca 
acccatcgc ttctctgtg gactacctg ggctcggtc 
ggtctaacc gacaaagtc acaagtctc gcaggcctc 
caagggccg ctcaaagac ctctatttc gcctacaaa 
aggacgaca agggccaag acactcacc gggaagttg 
gagatttcc ggccaaggg ctcaaagtg caataccaa 
ggcgaaaag ggcgacttg gagcaaatg aattcgttt 
ccgaccatt gctgtgtgg tcggcggtc aagtttgtg 
atacataag acgagttat gcttttttg ccactgatt 
accgatcct gataatatt gataagaaa gcattgttt 
cgggagttg agcgatggg gagaagaag tttatcaat 
ggggagacg cattcgcca ctttttgca gtcgtgatg 
agaaaaatc gggcttcag aagcaacta gagtgccac 
ggtttcgtc tgtcaaaca tccgaagac gcgatcgtc 
atcgctgca actctctac aagtcatta atggcccac 
atgaaagca aaagagaaa cggccgaaa aaccgcaac 
ggaatcact tgtatttca acaacttcg agcactttt 
aacgaaaaa tcggtccca gtccgacca ccgcgcaaa 
aaacgaagc acgacttcg tccataatt agcgaaaat 
gaacagctt gatgtgtct gatactcaa cccctgatt 
cagaaaaag aacgtccgg aagagtata aagtcgcgg 
ccggcgccc aaaaccccg gactttgat gcaattttg 
ccgtatgaa gagccggtt aaaccggag gaaatcaaa 
ccgaagacg ttttccgat cagatcaat acgtttatg 
agtcgggaa cagaaacaa atcacggcg gaaattaag 
caaatgatg aacgagaaa gtgatcaag ccggcggaa 
agtggcgaa gggaatagt ttgaggaag aaggataat 
tcgggtgat atcctgacg aaggtgacg atacccagg 
tcggggagt tttctcaat gctgggggt ctgacgcgg 
tataaatcg aaagtttca agaagcaac ggccaagca 
accggcggt tcccctctc ggcttcaac gaactcttc 
aacgaattc cgcctccag gagggcctc cactccatg 
gacgaaatc ctcggcgta ataatcgac cccgaagga 
atgtccttc aacgacctc aaacccatc tacaaggag 
ttcctcctc aagctctcc gtcaccctc acaaaagac 
gaactctac cagcgctcc aaatcgata atgcgccgc 
caaaaaaag aaactcctc cgccgaaac agcctccaa 
acaaagccc acccaccac atcctcgtc ggcagcaaa 
ttcaagcgt ctcaagcac atcttccgc aaaaacctc 
aaactcaag ctgggccgc aacaagtcc gagtccgac 
cccggcccc gggaaatcc cccgagagc agcatctcg 
acgtcgagc tacgacact cgccagttt tcgccgcgc 
cacgagccc aagccgaag aagcgcgac cgcgtctcc 
acctccgaa gagagcgac tttttcagc ctgcggcgc 
aggaaaatg gcggggaat cacaaccac caccagaat 
cggaactct tcgagcggc tacgtctcg tgctcggaa 
tgctcctac gacagcgac acgtgcact tgcgtctcg 
gccgataag tgctactgc tcgctggga aataaacaa 
aacgcgagg aaaaatcaa tgtgattgc aatctggac 
ggtccggtg acgttctgc gggtgtgat accgactca 
tgcaccgaa agcaataaa tgttactgt cagaacccg 
gccaggagt cggacgatt caggagttg aagcggcgc 
agtttgaag aaagacagg aagttgtgc aggagggcg 
tcgaattcg aggagtacg aagagtttg gagtacatt 
gggccgtat tatgagaag ttgggctcg aaaaggaag 
aattataac ggggtggcg gtggattat gagctgttc 
agtgtgagg agcgggaga gggaggaat gaccggaca 
ttgcaatcg actgccatt tccggcgcc tgcaccgaa 
gccctctct gtgaaaaaa tccgccgaa attgccgcc 
cttttcgcc gacattaaa ctaagtcaa acgacagac 
atcgcccac ttgacgccc cgaacttgc gacatggac 
acgattatt tcctcacga aatgcgtac aataaactg 
aaatcgcaa cacgaggcc aaaaaaatc atacagaac 
ggcctttat gtgagaaat aatccgaaa atgtacagt 
gcgaggaac ggactctac actattcag agccaatcg 
gatgaatcc agacgctcg agtttgagt gaggagaag 
aattatttt gatttgacg aagaaaaat agcagtaat 
ttggagaat tccttgggg tatttgccc taa
    mRNA sequence
    • >TC015652 RA
      catttcaaa aaccccaac taaattgct tcactcact 
cgctcaaat tgccaaaac ccaatcaat caaacttgc 
ggtcggcac ccattatca gtcccaacc gcaacagac 
ccatctcca accatccca aatcaaatc tcatcgaaa 
gtgaccaaa tcccaacca tttaaaaaa ccacaccag 
caaacacaa ccgttttcc tccacagca ccgtgactg 
aaattcact cactccacg ccaaaagtc agtcacttg 
caagaatcg gcgctgagt gctcccgtg ctataccga 
gtgagttga acgcgcgat gagacgctg agctgtgct 
tttgtgaaa ttatgtgac cctttgggc taccagatg 
cgggattat ctcaaagct gccgatacc cacccacga 
tgataatca tggacagcg tcgtcatcc cccgtcccc 
ccgccctcc ctccagtcc ccctcgccc ccccaaccc 
ccaaacccc ccagtctaa cgagcacaa catccggtt 
ttcgcctaa tggaggacc aaggcgaca ccggaagcg 
tctattcca tctacaagc aaaaaatcg acgcaatgt 
tcgagtccg attcgagca cgaacacaa aaaacagcg 
tccaagcgc gcatcgaga aaatgttca gcgacgtcg 
cccaagaca atggtatta ttctgagcg atgggacaa 
cccatcgct tctctgtgg actacctgg gctcggtcg 
gtctaaccg acaaagtca caagtctcg caggcctcc 
aagggccgc tcaaagacc tctatttcg cctacaaaa 
ggacgacaa gggccaaga cactcaccg ggaagttgg 
agatttccg gccaagggc tcaaagtgc aataccaag 
gcgaaaagg gcgacttgg agcaaatga attcgtttc 
cgaccattg ctgtgtggt cggcggtca agtttgtga 
tacataaga cgagttatg cttttttgc cactgatta 
ccgatcctg ataatattg ataagaaag cattgtttc 
gggagttga gcgatgggg agaagaagt ttatcaatg 
gggagacgc attcgccac tttttgcag tcgtgatga 
gaaaaatcg ggcttcaga agcaactag agtgccacg 
gtttcgtct gtcaaacat ccgaagacg cgatcgtca 
tcgctgcaa ctctctaca agtcattaa tggcccaca 
tgaaagcaa aagagaaac ggccgaaaa accgcaacg 
gaatcactt gtatttcaa caacttcga gcactttta 
acgaaaaat cggtcccag tccgaccac cgcgcaaaa 
aacgaagca cgacttcgt ccataatta gcgaaaatg 
aacagcttg atgtgtctg atactcaac ccctgattc 
agaaaaaga acgtccgga agagtataa agtcgcggc 
cggcgccca aaaccccgg actttgatg caattttgc 
cgtatgaag agccggtta aaccggagg aaatcaaac 
cgaagacgt tttccgatc agatcaata cgtttatga 
gtcgggaac agaaacaaa tcacggcgg aaattaagc 
aaatgatga acgagaaag tgatcaagc cggcggaaa 
gtggcgaag ggaatagtt tgaggaaga aggataatt 
cgggtgata tcctgacga aggtgacga tacccaggt 
cggggagtt ttctcaatg ctgggggtc tgacgcggt 
ataaatcga aagtttcaa gaagcaacg gccaagcaa 
ccggcggtt cccctctcg gcttcaacg aactcttca 
acgaattcc gcctccagg agggcctcc actccatgg 
acgaaatcc tcggcgtaa taatcgacc ccgaaggaa 
tgtccttca acgacctca aacccatct acaaggagt 
tcctcctca agctctccg tcaccctca caaaagacg 
aactctacc agcgctcca aatcgataa tgcgccgcc 
aaaaaaaga aactcctcc gccgaaaca gcctccaaa 
caaagccca cccaccaca tcctcgtcg gcagcaaat 
tcaagcgtc tcaagcaca tcttccgca aaaacctca 
aactcaagc tgggccgca acaagtccg agtccgacc 
ccggccccg ggaaatccc ccgagagca gcatctcga 
cgtcgagct acgacactc gccagtttt cgccgcgcc 
acgagccca agccgaaga agcgcgacc gcgtctcca 
cctccgaag agagcgact ttttcagcc tgcggcgca 
ggaaaatgg cggggaatc acaaccacc accagaatc 
ggaactctt cgagcggct acgtctcgt gctcggaat 
gctcctacg acagcgaca cgtgcactt gcgtctcgg 
ccgataagt gctactgct cgctgggaa ataaacaaa 
acgcgagga aaaatcaat gtgattgca atctggacg 
gtccggtga cgttctgcg ggtgtgata ccgactcat 
gcaccgaaa gcaataaat gttactgtc agaacccgg 
ccaggagtc ggacgattc aggagttga agcggcgca 
gtttgaaga aagacagga agttgtgca ggagggcgt 
cgaattcga ggagtacga agagtttgg agtacattg 
ggccgtatt atgagaagt tgggctcga aaaggaaga 
attataacg gggtggcgg tggattatg agctgttca 
gtgtgagga gcgggagag ggaggaatg accggacat 
tgcaatcga ctgccattt ccggcgcct gcaccgaag 
ccctctctg tgaaaaaat ccgccgaaa ttgccgccc 
ttttcgccg acattaaac taagtcaaa cgacagaca 
tcgcccact tgacgcccc gaacttgcg acatggaca 
cgattattt cctcacgaa atgcgtaca ataaactga 
aatcgcaac acgaggcca aaaaaatca tacagaacg 
gcctttatg tgagaaata atccgaaaa tgtacagtg 
cgaggaacg gactctaca ctattcaga gccaatcgg 
atgaatcca gacgctcga gtttgagtg aggagaaga 
attattttg atttgacga agaaaaata gcagtaatt 
tggagaatt ccttggggt atttgccct aaatgatgt 
gtacagtga gggataaaa tgccatttc tttcgtttt 
ggggggttt gagttgagg tagggttgt tagagggga 
gaaatttta attaagtgc aataatatg tgcaatatt 
gttatttat gtgcaataa tttatcgtt aaggaataa 
agattttat ctcaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY