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Gene information
General information
TC015652 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4144714..4176412
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015652
    iBeetle screen iB_05861

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015652 RA
      MIIMDSVVI PRPPALPPV PLAPPTPKP PSLTSTTSG 
      FRLMEDQGD TGSVYSIYK QKIDAMFES DSSTNTKNS 
      VQARIEKMF SDVAQDNGI ILSDGTTHR FSVDYLGSV 
      GLTDKVTSL AGLQGPLKD LYFAYKRTT RAKTLTGKL 
      EISGQGLKV QYQGEKGDL EQMNSFPTI AVWSAVKFV 
      IHKTSYAFL PLITDPDNI DKKALFREL SDGEKKFIN 
      GETHSPLFA VVMRKIGLQ KQLECHGFV CQTSEDAIV 
      IAATLYKSL MAHMKAKEK RPKNRNGIT CISTTSSTF 
      NEKSVPVRP PRKKRSTTS SIISENEQL DVSDTQPLI 
      QKKNVRKSI KSRPAPKTP DFDAILPYE EPVKPEEIK 
      PKTFSDQIN TFMSREQKQ ITAEIKQMM NEKVIKPAE 
      SGEGNSLRK KDNSGDILT KVTIPRSGS FLNAGGLTR 
      YKSKVSRSN GQATGGSPL GFNELFNEF RLQEGLHSM 
      DEILGVIID PEGMSFNDL KPIYKEFLL KLSVTLTKD 
      ELYQRSKSI MRRQKKKLL RRNSLQTKP THHILVGSK 
      FKRLKHIFR KNLKLKLGR NKSESDPGP GKSPESSIS 
      TSSYDTRQF SPRHEPKPK KRDRVSTSE ESDFFSLRR 
      RKMAGNHNH HQNRNSSSG YVSCSECSY DSDTCTCVS 
      ADKCYCSLG NKQNARKNQ CDCNLDGPV TFCGCDTDS 
      CTESNKCYC QNPARSRTI QELKRRSLK KDRKLCRRA 
      SNSRSTKSL EYIGPYYEK LGSKRKNYN GVAVDYELF 
      SVRSGRGRN DRTLQSTAI SGACTEALS VKKSAEIAA 
      LFADIKLSQ TTDIAHLTP RTCDMDTII SSRNAYNKL 
      KSQHEAKKI IQNGLYVRN NPKMYSARN GLYTIQSQS 
      DESRRSSLS EEKNYFDLT KKNSSNLEN SLGYLP
    CDS sequence
    • >TC015652 RA
      atgataatc atggacagc gtcgtcatc ccccgtccc 
      cccgccctc cctccagtc cccctcgcc cccccaacc 
      cccaaaccc cccagtcta acgagcaca acatccggt 
      tttcgccta atggaggac caaggcgac accggaagc 
      gtctattcc atctacaag caaaaaatc gacgcaatg 
      ttcgagtcc gattcgagc acgaacaca aaaaacagc 
      gtccaagcg cgcatcgag aaaatgttc agcgacgtc 
      gcccaagac aatggtatt attctgagc gatgggaca 
      acccatcgc ttctctgtg gactacctg ggctcggtc 
      ggtctaacc gacaaagtc acaagtctc gcaggcctc 
      caagggccg ctcaaagac ctctatttc gcctacaaa 
      aggacgaca agggccaag acactcacc gggaagttg 
      gagatttcc ggccaaggg ctcaaagtg caataccaa 
      ggcgaaaag ggcgacttg gagcaaatg aattcgttt 
      ccgaccatt gctgtgtgg tcggcggtc aagtttgtg 
      atacataag acgagttat gcttttttg ccactgatt 
      accgatcct gataatatt gataagaaa gcattgttt 
      cgggagttg agcgatggg gagaagaag tttatcaat 
      ggggagacg cattcgcca ctttttgca gtcgtgatg 
      agaaaaatc gggcttcag aagcaacta gagtgccac 
      ggtttcgtc tgtcaaaca tccgaagac gcgatcgtc 
      atcgctgca actctctac aagtcatta atggcccac 
      atgaaagca aaagagaaa cggccgaaa aaccgcaac 
      ggaatcact tgtatttca acaacttcg agcactttt 
      aacgaaaaa tcggtccca gtccgacca ccgcgcaaa 
      aaacgaagc acgacttcg tccataatt agcgaaaat 
      gaacagctt gatgtgtct gatactcaa cccctgatt 
      cagaaaaag aacgtccgg aagagtata aagtcgcgg 
      ccggcgccc aaaaccccg gactttgat gcaattttg 
      ccgtatgaa gagccggtt aaaccggag gaaatcaaa 
      ccgaagacg ttttccgat cagatcaat acgtttatg 
      agtcgggaa cagaaacaa atcacggcg gaaattaag 
      caaatgatg aacgagaaa gtgatcaag ccggcggaa 
      agtggcgaa gggaatagt ttgaggaag aaggataat 
      tcgggtgat atcctgacg aaggtgacg atacccagg 
      tcggggagt tttctcaat gctgggggt ctgacgcgg 
      tataaatcg aaagtttca agaagcaac ggccaagca 
      accggcggt tcccctctc ggcttcaac gaactcttc 
      aacgaattc cgcctccag gagggcctc cactccatg 
      gacgaaatc ctcggcgta ataatcgac cccgaagga 
      atgtccttc aacgacctc aaacccatc tacaaggag 
      ttcctcctc aagctctcc gtcaccctc acaaaagac 
      gaactctac cagcgctcc aaatcgata atgcgccgc 
      caaaaaaag aaactcctc cgccgaaac agcctccaa 
      acaaagccc acccaccac atcctcgtc ggcagcaaa 
      ttcaagcgt ctcaagcac atcttccgc aaaaacctc 
      aaactcaag ctgggccgc aacaagtcc gagtccgac 
      cccggcccc gggaaatcc cccgagagc agcatctcg 
      acgtcgagc tacgacact cgccagttt tcgccgcgc 
      cacgagccc aagccgaag aagcgcgac cgcgtctcc 
      acctccgaa gagagcgac tttttcagc ctgcggcgc 
      aggaaaatg gcggggaat cacaaccac caccagaat 
      cggaactct tcgagcggc tacgtctcg tgctcggaa 
      tgctcctac gacagcgac acgtgcact tgcgtctcg 
      gccgataag tgctactgc tcgctggga aataaacaa 
      aacgcgagg aaaaatcaa tgtgattgc aatctggac 
      ggtccggtg acgttctgc gggtgtgat accgactca 
      tgcaccgaa agcaataaa tgttactgt cagaacccg 
      gccaggagt cggacgatt caggagttg aagcggcgc 
      agtttgaag aaagacagg aagttgtgc aggagggcg 
      tcgaattcg aggagtacg aagagtttg gagtacatt 
      gggccgtat tatgagaag ttgggctcg aaaaggaag 
      aattataac ggggtggcg gtggattat gagctgttc 
      agtgtgagg agcgggaga gggaggaat gaccggaca 
      ttgcaatcg actgccatt tccggcgcc tgcaccgaa 
      gccctctct gtgaaaaaa tccgccgaa attgccgcc 
      cttttcgcc gacattaaa ctaagtcaa acgacagac 
      atcgcccac ttgacgccc cgaacttgc gacatggac 
      acgattatt tcctcacga aatgcgtac aataaactg 
      aaatcgcaa cacgaggcc aaaaaaatc atacagaac 
      ggcctttat gtgagaaat aatccgaaa atgtacagt 
      gcgaggaac ggactctac actattcag agccaatcg 
      gatgaatcc agacgctcg agtttgagt gaggagaag 
      aattatttt gatttgacg aagaaaaat agcagtaat 
      ttggagaat tccttgggg tatttgccc taa
    mRNA sequence
    • >TC015652 RA
      catttcaaa aaccccaac taaattgct tcactcact 
      cgctcaaat tgccaaaac ccaatcaat caaacttgc 
      ggtcggcac ccattatca gtcccaacc gcaacagac 
      ccatctcca accatccca aatcaaatc tcatcgaaa 
      gtgaccaaa tcccaacca tttaaaaaa ccacaccag 
      caaacacaa ccgttttcc tccacagca ccgtgactg 
      aaattcact cactccacg ccaaaagtc agtcacttg 
      caagaatcg gcgctgagt gctcccgtg ctataccga 
      gtgagttga acgcgcgat gagacgctg agctgtgct 
      tttgtgaaa ttatgtgac cctttgggc taccagatg 
      cgggattat ctcaaagct gccgatacc cacccacga 
      tgataatca tggacagcg tcgtcatcc cccgtcccc 
      ccgccctcc ctccagtcc ccctcgccc ccccaaccc 
      ccaaacccc ccagtctaa cgagcacaa catccggtt 
      ttcgcctaa tggaggacc aaggcgaca ccggaagcg 
      tctattcca tctacaagc aaaaaatcg acgcaatgt 
      tcgagtccg attcgagca cgaacacaa aaaacagcg 
      tccaagcgc gcatcgaga aaatgttca gcgacgtcg 
      cccaagaca atggtatta ttctgagcg atgggacaa 
      cccatcgct tctctgtgg actacctgg gctcggtcg 
      gtctaaccg acaaagtca caagtctcg caggcctcc 
      aagggccgc tcaaagacc tctatttcg cctacaaaa 
      ggacgacaa gggccaaga cactcaccg ggaagttgg 
      agatttccg gccaagggc tcaaagtgc aataccaag 
      gcgaaaagg gcgacttgg agcaaatga attcgtttc 
      cgaccattg ctgtgtggt cggcggtca agtttgtga 
      tacataaga cgagttatg cttttttgc cactgatta 
      ccgatcctg ataatattg ataagaaag cattgtttc 
      gggagttga gcgatgggg agaagaagt ttatcaatg 
      gggagacgc attcgccac tttttgcag tcgtgatga 
      gaaaaatcg ggcttcaga agcaactag agtgccacg 
      gtttcgtct gtcaaacat ccgaagacg cgatcgtca 
      tcgctgcaa ctctctaca agtcattaa tggcccaca 
      tgaaagcaa aagagaaac ggccgaaaa accgcaacg 
      gaatcactt gtatttcaa caacttcga gcactttta 
      acgaaaaat cggtcccag tccgaccac cgcgcaaaa 
      aacgaagca cgacttcgt ccataatta gcgaaaatg 
      aacagcttg atgtgtctg atactcaac ccctgattc 
      agaaaaaga acgtccgga agagtataa agtcgcggc 
      cggcgccca aaaccccgg actttgatg caattttgc 
      cgtatgaag agccggtta aaccggagg aaatcaaac 
      cgaagacgt tttccgatc agatcaata cgtttatga 
      gtcgggaac agaaacaaa tcacggcgg aaattaagc 
      aaatgatga acgagaaag tgatcaagc cggcggaaa 
      gtggcgaag ggaatagtt tgaggaaga aggataatt 
      cgggtgata tcctgacga aggtgacga tacccaggt 
      cggggagtt ttctcaatg ctgggggtc tgacgcggt 
      ataaatcga aagtttcaa gaagcaacg gccaagcaa 
      ccggcggtt cccctctcg gcttcaacg aactcttca 
      acgaattcc gcctccagg agggcctcc actccatgg 
      acgaaatcc tcggcgtaa taatcgacc ccgaaggaa 
      tgtccttca acgacctca aacccatct acaaggagt 
      tcctcctca agctctccg tcaccctca caaaagacg 
      aactctacc agcgctcca aatcgataa tgcgccgcc 
      aaaaaaaga aactcctcc gccgaaaca gcctccaaa 
      caaagccca cccaccaca tcctcgtcg gcagcaaat 
      tcaagcgtc tcaagcaca tcttccgca aaaacctca 
      aactcaagc tgggccgca acaagtccg agtccgacc 
      ccggccccg ggaaatccc ccgagagca gcatctcga 
      cgtcgagct acgacactc gccagtttt cgccgcgcc 
      acgagccca agccgaaga agcgcgacc gcgtctcca 
      cctccgaag agagcgact ttttcagcc tgcggcgca 
      ggaaaatgg cggggaatc acaaccacc accagaatc 
      ggaactctt cgagcggct acgtctcgt gctcggaat 
      gctcctacg acagcgaca cgtgcactt gcgtctcgg 
      ccgataagt gctactgct cgctgggaa ataaacaaa 
      acgcgagga aaaatcaat gtgattgca atctggacg 
      gtccggtga cgttctgcg ggtgtgata ccgactcat 
      gcaccgaaa gcaataaat gttactgtc agaacccgg 
      ccaggagtc ggacgattc aggagttga agcggcgca 
      gtttgaaga aagacagga agttgtgca ggagggcgt 
      cgaattcga ggagtacga agagtttgg agtacattg 
      ggccgtatt atgagaagt tgggctcga aaaggaaga 
      attataacg gggtggcgg tggattatg agctgttca 
      gtgtgagga gcgggagag ggaggaatg accggacat 
      tgcaatcga ctgccattt ccggcgcct gcaccgaag 
      ccctctctg tgaaaaaat ccgccgaaa ttgccgccc 
      ttttcgccg acattaaac taagtcaaa cgacagaca 
      tcgcccact tgacgcccc gaacttgcg acatggaca 
      cgattattt cctcacgaa atgcgtaca ataaactga 
      aatcgcaac acgaggcca aaaaaatca tacagaacg 
      gcctttatg tgagaaata atccgaaaa tgtacagtg 
      cgaggaacg gactctaca ctattcaga gccaatcgg 
      atgaatcca gacgctcga gtttgagtg aggagaaga 
      attattttg atttgacga agaaaaata gcagtaatt 
      tggagaatt ccttggggt atttgccct aaatgatgt 
      gtacagtga gggataaaa tgccatttc tttcgtttt 
      ggggggttt gagttgagg tagggttgt tagagggga 
      gaaatttta attaagtgc aataatatg tgcaatatt 
      gttatttat gtgcaataa tttatcgtt aaggaataa 
      agattttat ctcaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY