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Gene information
General information
TC015649 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4109519..4114537
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015649
    iBeetle screen iB_05859

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015649 RA
      MSDKNVRKE SPDDKPQID GNMEGNSQT KSSKKRNKK 
      KQHQPENEA ELLSNFTKP LNTISIQGL RAAMEALTM 
      NQKPAKTPE EALTKSYQF WNTQPVPKM DEKITVNEA 
      IEPDRQVAE IRAEPYSLP DGFAWDTLN LDEPLVLKE 
      LYTLLNENY VEDDDCMFR FDYQPEFLK WALQPPGWR 
      KDWHCGVRV VKSGRLVGF ISAIPATLK IYEKTQKMV 
      EINFLCVHK RLRSKRVAP VLIREITRR VHLTGLFQA 
      VYTAGIVLP KPVGTCRYW HRSLNPKKL IEVKFSHLS 
      RNMTMQRTL KLYKLPDSP RTPGFRKLT EADYLRKFD 
      LSPLFTEED FVHWFLPRP DIVDSFVVE KGGKVTDFV 
      SYYTLPSTV MHHPVHTSL KAAYSFYNV SGETPWVDL 
      MNDALISAK QSNFDVFNA LDLMDNREF LETLKFGIG 
      DGNLQYYLY NWKCPSMAP HRMGLVLQ
    CDS sequence
    • >TC015649 RA
      atgtctgac aagaacgtt cgcaaagag tcgccggac 
      gacaagccc caaatcgac ggaaacatg gagggaaac 
      tcgcaaacg aaaagttcg aaaaaacgc aacaagaag 
      aaacaacac caaccggaa aacgaagcc gaactgtta 
      tcaaacttc accaagccg ctgaacacc atctcgatc 
      cagggcctg cgcgccgcc atggaggcc ctcaccatg 
      aaccagaag ccggccaag acccccgag gaggccctc 
      accaagtcc taccagttc tggaacacc cagcccgtg 
      cctaagatg gacgagaag atcaccgtc aacgaggcc 
      atcgagccc gaccgccag gtggctgag atccgggcg 
      gagccctac agcctgccc gacggcttc gcctgggac 
      accctcaac ctggacgag cccctcgtg ctcaaggag 
      ctctacacg ctgctgaac gagaactac gtcgaagac 
      gacgactgc atgttccgc ttcgactac cagccggag 
      ttcctcaag tgggcgctg cagcccccc ggctggcgc 
      aaggactgg cactgcgga gtccgagtt gttaaaagc 
      gggcgactt gtcgggttt atctccgct attccggca 
      acgttgaag atttacgaa aaaacgcag aaaatggtc 
      gagattaat tttctctgc gtgcataag agattgcgc 
      tcgaagcga gtcgctccg gttctgatc cgggagatt 
      acgagacgg gttcatctc accggactt ttccaggcg 
      gtttacacg gctggaatt gttttaccg aaaccggtg 
      ggcacgtgt cggtattgg cacagatcg ctcaatccg 
      aagaaacta atcgaagtg aaattctca catttgtcc 
      aggaatatg acgatgcaa cggacactc aaactttac 
      aaattgcct gatagtcca aggacgcca gggtttagg 
      aaattgacc gaagctgac tatttgcgg aagttcgac 
      ttgtccccc cttttcacc gaagaggac ttcgtgcac 
      tggttcctc cctcggccc gatatcgtg gacagtttc 
      gtggtggag aaagggggc aaagtgacc gacttcgtc 
      agctactac acgctcccc tccacagtg atgcaccac 
      ccggttcac acaagttta aaggcggcg tattcgttt 
      tacaacgtt tcgggggaa accccctgg gtggatttg 
      atgaatgac gcgttaatt tcggcgaaa cagtcaaac 
      ttcgacgtt tttaacgcc ttggatctg atggataat 
      cgcgagttt ttggagacg ctcaaattc gggattgga 
      gatggaaat ttgcaatat tatttgtac aattggaag 
      tgtccgagc atggcaccg catcggatg ggtctagtc 
      ttgcaataa
    mRNA sequence
    • >TC015649 RA
      atgtctgac aagaacgtt cgcaaagag tcgccggac 
      gacaagccc caaatcgac ggaaacatg gagggaaac 
      tcgcaaacg aaaagttcg aaaaaacgc aacaagaag 
      aaacaacac caaccggaa aacgaagcc gaactgtta 
      tcaaacttc accaagccg ctgaacacc atctcgatc 
      cagggcctg cgcgccgcc atggaggcc ctcaccatg 
      aaccagaag ccggccaag acccccgag gaggccctc 
      accaagtcc taccagttc tggaacacc cagcccgtg 
      cctaagatg gacgagaag atcaccgtc aacgaggcc 
      atcgagccc gaccgccag gtggctgag atccgggcg 
      gagccctac agcctgccc gacggcttc gcctgggac 
      accctcaac ctggacgag cccctcgtg ctcaaggag 
      ctctacacg ctgctgaac gagaactac gtcgaagac 
      gacgactgc atgttccgc ttcgactac cagccggag 
      ttcctcaag tgggcgctg cagcccccc ggctggcgc 
      aaggactgg cactgcgga gtccgagtt gttaaaagc 
      gggcgactt gtcgggttt atctccgct attccggca 
      acgttgaag atttacgaa aaaacgcag aaaatggtc 
      gagattaat tttctctgc gtgcataag agattgcgc 
      tcgaagcga gtcgctccg gttctgatc cgggagatt 
      acgagacgg gttcatctc accggactt ttccaggcg 
      gtttacacg gctggaatt gttttaccg aaaccggtg 
      ggcacgtgt cggtattgg cacagatcg ctcaatccg 
      aagaaacta atcgaagtg aaattctca catttgtcc 
      aggaatatg acgatgcaa cggacactc aaactttac 
      aaattgcct gatagtcca aggacgcca gggtttagg 
      aaattgacc gaagctgac tatttgcgg aagttcgac 
      ttgtccccc cttttcacc gaagaggac ttcgtgcac 
      tggttcctc cctcggccc gatatcgtg gacagtttc 
      gtggtggag aaagggggc aaagtgacc gacttcgtc 
      agctactac acgctcccc tccacagtg atgcaccac 
      ccggttcac acaagttta aaggcggcg tattcgttt 
      tacaacgtt tcgggggaa accccctgg gtggatttg 
      atgaatgac gcgttaatt tcggcgaaa cagtcaaac 
      ttcgacgtt tttaacgcc ttggatctg atggataat 
      cgcgagttt ttggagacg ctcaaattc gggattgga 
      gatggaaat ttgcaatat tatttgtac aattggaag 
      tgtccgagc atggcaccg catcggatg ggtctagtc 
      ttgcaataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY