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Gene information
General information
TC015629 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3731346..3732850
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015629
    iBeetle screen iB_05852

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    antenna everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen posterior everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 33.33% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Adult morphology
    mesothorax coxa orientation irregular - Number of animals showing the phenotype: 4

    Comment: the coxae and the posterior mesobasisternum are slightly extruding to the ventral side, making the posterior process of the mesobasisternum being disconnected from the T3-intercoxal process

    mesobasisternum shape irregular - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015629 RA
      MLDDAHYIC AETPETYRC ELCNFTTDF KLILHQHAQ 
NFHHNSPGN ETSKKIQKY KCYKCDFKT FSVLLWFKH 
NVRKNLEIG EFTSPQATR FECTYCPFK ATQKGSLKS 
HILRKHTPS DEIQWSQCT QCAFKTKLK TSLKRHIKR 
QHTNSDEVQ IKCDLCNYT TNRQDNLRR HKSYNHLPP 
SVIHECREC PFKTKHGGD LKRHHIRHH VASEAISWH 
HCHLCPHRS KTQSDLKSH IRLKHEGVK QETKFSCDF 
CPYKTSQQI SLKRHIIRR HTAPEEVEW FECDQCNYK 
GKCKEYLRD HKKMQHSDS SVQKLACNE CPFKTISED 
DLKKHVAKR HSSKKLHEC PHCFFTTHL PFLLKRHIV 
VEHTEPGDI KWFQCEQCD YKGKLKYNL TRHVKSVHK 
SGEAKMIHC DLCEYKSKK RENVKRHKI SIHLTKQKI 
Q
    CDS sequence
    • >TC015629 RA
      atgcttgat gatgcccac tacatatgt gccgaaacg 
cctgaaact tacagatgc gaactttgc aatttcaca 
accgacttc aaactcatt ttgcaccaa cacgcccaa 
aacttccat cacaattca ccggggaac gaaaccagc 
aaaaaaatt caaaaatat aaatgttac aaatgcgat 
tttaaaacg ttctcagta ctgttgtgg ttcaagcac 
aatgttcgc aaaaatcta gaaattggc gaatttaca 
tcacctcaa gcaacgcgt tttgaatgc acttattgc 
ccatttaaa gccacccaa aaagggtcg ttaaaatcg 
cacattctc cgcaaacac accccatct gacgaaatc 
caatggtcc caatgcaca caatgcgct ttcaaaacc 
aaactaaaa acatcgcta aaaagacac atcaagcgc 
caacacacc aacagtgac gaagttcaa atcaagtgt 
gacctgtgc aattataca acaaaccgc caagataat 
ttaaggaga cacaaaagt tacaatcat ttgccacct 
agtgttatc cacgagtgt agggaatgt ccgttcaag 
acgaaacat gggggcgac ttgaagcgc caccacatc 
agacaccac gtagcttcc gaggccata agttggcac 
cactgtcac ctttgcccc caccggtct aaaacccag 
tcagacttg aaatcacac attcggtta aaacacgag 
ggcgttaaa caggagacc aagttctct tgcgatttt 
tgtccgtac aagacctcg caacaaatt tctctcaaa 
cgccacatc atcagacgc cacacagcg cctgaagaa 
gtcgagtgg tttgagtgt gaccaatgc aactacaag 
ggcaaatgt aaagaatat ctgcgtgat cacaaaaaa 
atgcaacac tctgatagc agtgtccag aagttggct 
tgcaatgag tgtccgttt aagacaatc tctgaggac 
gatttgaaa aaacatgtc gctaaaaga cactcttcg 
aagaagttg cacgagtgt ccgcattgt tttttcaca 
acgcatctg ccgttttta ctgaaacgg catatagtt 
gttgaacac actgagcct ggggatatc aagtggttc 
cagtgcgag cagtgtgac tataaaggc aaacttaag 
tataatttg acgagacat gtcaagtcg gtgcataag 
agtggcgag gcgaagatg attcactgt gatttgtgc 
gagtacaag tcgaagaag agggagaat gtgaagagg 
cataagatc agtatacac ctaactaaa caaaaaatt 
cagtag
    mRNA sequence
    • >TC015629 RA
      gtcaaacaa aaattatat tttatctca aaaaacgtt 
tcaaaaatg tggtgaaaa acctagaat tattataca 
aataaagaa aaatgcttg atgatgccc actacatat 
gtgccgaaa cgcctgaaa cttacagat gcgaacttt 
gcaatttca caaccgact tcaaactca ttttgcacc 
aacacgccc aaaacttcc atcacaatt caccgggga 
acgaaacca gcaaaaaaa ttcaaaaat ataaatgtt 
acaaatgcg attttaaaa cgttctcag tactgttgt 
ggttcaagc acaatgttc gcaaaaatc tagaaattg 
gcgaattta catcacctc aagcaacgc gttttgaat 
gcacttatt gcccattta aagccaccc aaaaagggt 
cgttaaaat cgcacattc tccgcaaac acaccccat 
ctgacgaaa tccaatggt cccaatgca cacaatgcg 
ctttcaaaa ccaaactaa aaacatcgc taaaaagac 
acatcaagc gccaacaca ccaacagtg acgaagttc 
aaatcaagt gtgacctgt gcaattata caacaaacc 
gccaagata atttaagga gacacaaaa gttacaatc 
atttgccac ctagtgtta tccacgagt gtagggaat 
gtccgttca agacgaaac atgggggcg acttgaagc 
gccaccaca tcagacacc acgtagctt ccgaggcca 
taagttggc accactgtc acctttgcc cccaccggt 
ctaaaaccc agtcagact tgaaatcac acattcggt 
taaaacacg agggcgtta aacaggaga ccaagttct 
cttgcgatt tttgtccgt acaagacct cgcaacaaa 
tttctctca aacgccaca tcatcagac gccacacag 
cgcctgaag aagtcgagt ggtttgagt gtgaccaat 
gcaactaca agggcaaat gtaaagaat atctgcgtg 
atcacaaaa aaatgcaac actctgata gcagtgtcc 
agaagttgg cttgcaatg agtgtccgt ttaagacaa 
tctctgagg acgatttga aaaaacatg tcgctaaaa 
gacactctt cgaagaagt tgcacgagt gtccgcatt 
gttttttca caacgcatc tgccgtttt tactgaaac 
ggcatatag ttgttgaac acactgagc ctggggata 
tcaagtggt tccagtgcg agcagtgtg actataaag 
gcaaactta agtataatt tgacgagac atgtcaagt 
cggtgcata agagtggcg aggcgaaga tgattcact 
gtgatttgt gcgagtaca agtcgaaga agagggaga 
atgtgaaga ggcataaga tcagtatac acctaacta 
aacaaaaaa ttcagtagg tggtttttt ttgtttaca 
gttgttatt tattacttg tatttacta tttgttaat 
aaaaattgg aaacctgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY