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Gene information
General information
TC033405
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015603,TC015602
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3346656..3350890
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033405
    iBeetle screen iB_05847

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033405 RA
      MRKEDEITK AFKWVEKNL GQVHILINN AGVAKEGLL 
      SNGDTEAWR NTFDVNVMG LCIATREAV KSMTANNIN 
      GHIIHINSV AGHKVVYFP GINVYTASK HAVTALAET 
      LRHEFLARG SKIKITSVS PGFVISEMT TLNTGYSPE 
      RQKMLKSQP ILKPEDIAD AVCYVLSTP ETVQSVKMV 
      LSLSRWMGK VAIVTGASS GIGAAIADA LVANGVIVA 
      GLARRSERI QERAKKLQG KLHAIRTDM RKEDEIIKA 
      FKWVEKNLG QVHILINNA GVSTESFLA NGDTEAWRN 
      IFDVNVMGL CIATREAVK SMTTNDING HIVHISSIA 
      GHKIVNVPG INAYTASKH AVTALAGTL RNEFLTLGS 
      KIKITSVSP GLVISEMTT LGTGYSAER QEMMKSQPI 
      LKPEDVADA VCYALSTPE NVEINELTI TPVGERI
    CDS sequence
    • >TC033405 RA
      atgagaaaa gaagacgaa attaccaaa gccttcaaa 
      tgggttgag aaaaatctc ggacaagtt cacatccta 
      atcaacaat gctggtgtt gctaaagaa ggtttgcta 
      tcaaacgga gacacggaa gcttggagg aatactttc 
      gatgtaaac gtcatggga ctgtgtatt gccacaaga 
      gaagcagtc aaatcaatg acagcaaac aacataaac 
      ggacatatc attcacatt aatagcgtt gcgggccat 
      aaagttgtg tacttcccc gggattaat gtttacaca 
      gccagcaaa catgcagtc acagctttg gccgagact 
      ttaaggcac gagtttctc gctcgtggg tcgaaaatc 
      aaaattaca agtgtcagt cctggattt gttataagc 
      gaaatgacg actctcaat accgggtat agtcctgaa 
      cgacagaaa atgctcaaa agtcaaccg attctgaag 
      cctgaagat attgcagat gccgtttgt tacgtatta 
      tctacacct gagaccgtc caatcagtt aaaatggtt 
      ctttcacta agcagatgg atgggcaaa gtagctata 
      gttacgggc gcaagttca ggaattggt gctgcaatt 
      gccgacgct ttggtagca aacggtgtt attgttgct 
      ggactcgct cgtcgctct gaacggatc caagagcgc 
      gctaaaaag ctccaaggc aaacttcac gctattaga 
      actgacatg agaaaagaa gacgaaatt attaaagcc 
      ttcaaatgg gttgaaaaa aatctcgga caagtccac 
      atcctaatc aacaatgct ggtgtttct acagaaagt 
      ttcctagca aacggagac accgaggct tggaggaac 
      attttcgat gtgaacgtc atgggactg tgcattgcc 
      acgagagaa gcggtgaaa tcaatgaca acaaacgac 
      ataaacggg catattgtc cacattagt agcattgcc 
      gggcataaa attgtgaat gttcccgga atcaatgcc 
      tacacagcc agcaaacat gcagtcacg gcattggcc 
      gggacttta cgaaacgag tttctcact cttgggtcg 
      aaaatcaaa attacaagc gtcagtccg ggattggtt 
      ataagcgaa atgacgact ctcggtact ggatatagc 
      gccgaacgg caggaaatg atgaaaagt caaccaatt 
      ttaaagcct gaagacgtt gcagatgca gtgtgctat 
      gcattgtcc acacccgaa aatgtcgag ataaacgaa 
      ctgacaatc acaccagtc ggcgaaaga atttaa
    mRNA sequence
    • >TC033405 RA
      gaaggcaag gaaggaaag ctccacgct gtcaaaact 
      gacatgaga aaagaagac gaaattacc aaagccttc 
      aaatgggtt gagaaaaat ctcggacaa gttcacatc 
      ctaatcaac aatgctggt gttgctaaa gaaggtttg 
      ctatcaaac ggagacacg gaagcttgg aggaatact 
      ttcgatgta aacgtcatg ggactgtgt attgccaca 
      agagaagca gtcaaatca atgacagca aacaacata 
      aacggacat atcattcac attaatagc gttgcgggc 
      cataaagtt gtgtacttc cccgggatt aatgtttac 
      acagccagc aaacatgca gtcacagct ttggccgag 
      actttaagg cacgagttt ctcgctcgt gggtcgaaa 
      atcaaaatt acaagtgtc agtcctgga tttgttata 
      agcgaaatg acgactctc aataccggg tatagtcct 
      gaacgacag aaaatgctc aaaagtcaa ccgattctg 
      aagcctgaa gatattgca gatgccgtt tgttacgta 
      ttatctaca cctgagacc gtccaatca gttaaaatg 
      gttctttca ctaagcaga tggatgggc aaagtagct 
      atagttacg ggcgcaagt tcaggaatt ggtgctgca 
      attgccgac gctttggta gcaaacggt gttattgtt 
      gctggactc gctcgtcgc tctgaacgg atccaagag 
      cgcgctaaa aagctccaa ggcaaactt cacgctatt 
      agaactgac atgagaaaa gaagacgaa attattaaa 
      gccttcaaa tgggttgaa aaaaatctc ggacaagtc 
      cacatccta atcaacaat gctggtgtt tctacagaa 
      agtttccta gcaaacgga gacaccgag gcttggagg 
      aacattttc gatgtgaac gtcatggga ctgtgcatt 
      gccacgaga gaagcggtg aaatcaatg acaacaaac 
      gacataaac gggcatatt gtccacatt agtagcatt 
      gccgggcat aaaattgtg aatgttccc ggaatcaat 
      gcctacaca gccagcaaa catgcagtc acggcattg 
      gccgggact ttacgaaac gagtttctc actcttggg 
      tcgaaaatc aaaattaca agcgtcagt ccgggattg 
      gttataagc gaaatgacg actctcggt actggatat 
      agcgccgaa cggcaggaa atgatgaaa agtcaacca 
      attttaaag cctgaagac gttgcagat gcagtgtgc 
      tatgcattg tccacaccc gaaaatgtc gagataaac 
      gaactgaca atcacacca gtcggcgaa agaatttaa 
      agtgtttta tttctctac agggtgatt ctcataaca 
      cttccagcc cagccaaca ccaaaataa acttcattc 
      aaaagtcat agtctttga agtttgatg ttttcactt 
      cacttgagc gtctttgaa caactttgg tttataggg 
      atatacatt ttttcataa tatttttct tgtatctgt 
      aaccgtact cgcaaggct ttaataaat ttcacgaaa 
      atgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY