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Gene information
General information
TC015598 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3305153..3306437
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015598
    iBeetle screen iB_05846

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015598 RA
      MLPFFEKLS FLYQPYVLA IVSIGYVLG ELGHYLIGV 
      TSKAIAMDL HFGDIACQF NSTEFSLAD LPATCDTAN 
      SSEICLSFN INGTPYCEW NYNGLGIDY QILAGPTFM 
      VVFTVVGVI LGIAADKYN RVRILAICT IIFSIAIIL 
      CGGVTEFWQ LVLLRMIMA AGESGCNPL ATGLLSDIF 
      PEKQRALAM SIFNWGIYG GYGIAFPVG RYVPPLNAW 
      GLGWRVTYY GTGIVALIV GLLTWFTLK EPPRQSIGE 
      DAEHDPNAK KITIWTIIL DPRIIMLCL AASLRHCGG 
      MCFAYNCDL YYQQYFPEY DLGWWLFAV TIIVGSIGV 
      VVGGIVSDV FVAKMGIRS RVMVLAISQ IIATPGAFG 
      SVYFNPTWA MIFLGLSYF FGEFRTK
    CDS sequence
    • >TC015598 RA
      atgttgccc ttcttcgag aagctcagc ttcctgtac 
      cagccctac gtcctggcc attgtctcc atcggctat 
      gtcctgggc gagctgggc cactaccta atcggcgtc 
      acctccaaa gccatcgcc atggacctc cacttcggc 
      gacatcgcc tgccagttc aacagcacc gagttctcc 
      ctcgccgac ctcccagcc acctgcgac accgccaac 
      agttccgag atctgcctc tccttcaac atcaacgga 
      actccttac tgcgaatgg aactacaac ggtctcggc 
      atcgactac caaatccta gccgggccc accttcatg 
      gtcgtcttc accgtcgtg ggcgtcatc ctcggcata 
      gctgccgac aagtacaac cgggtgcgc attctggcc 
      atttgtacc atcattttc tccatcgcc attatcctc 
      tgtgggggc gtgacggag ttctggcag ctggtgctg 
      ctgaggatg atcatggcg gctggggag tcggggtgc 
      aaccccctg gccacgggc ctgctctcg gacatcttc 
      cccgagaag cagcgcgcg ctggccatg tccatcttc 
      aactggggg atttatggg ggctacggg atcgccttc 
      cccgtgggg cgctatgtg cccccgctg aacgcctgg 
      ggcttgggc tggagagtc acctattac gggactggg 
      attgtggcg ctgatcgtg gggctgctc acctggttc 
      acgctgaag gaaccccca agacagtcg atcggggaa 
      gacgcagag catgatccc aatgccaag aaaatcacc 
      atttggacg attattctc gacccgagg atcattatg 
      ctgtgtttg gcggcgagt ctgaggcac tgtgggggc 
      atgtgtttc gcttacaac tgcgatctg tattaccag 
      cagtacttc cccgagtat gatctgggg tggtggctg 
      tttgctgtc actatcatt gttgggagt attggagtg 
      gtggttggg ggaatcgtc tcagatgtt ttcgttgcc 
      aaaatgggg ataaggtcg agagttatg gtacttgcc 
      atcagtcag attattgcc acaccggga gccttcggc 
      agtgtttat ttcaatccc acgtgggcg atgattttc 
      ttaggactg tcgtacttt ttcggtgag tttcgaaca 
      aaataa
    mRNA sequence
    • >TC015598 RA
      ccgttgtgg agagatgtc cgttgttcg gaataaaat 
      ttcggagaa aaaatttag attcgtgtt ttttcagtt 
      ctagcccaa gatgttgcc cttcttcga gaagctcag 
      cttcctgta ccagcccta cgtcctggc cattgtctc 
      catcggcta tgtcctggg cgagctggg ccactacct 
      aatcggcgt cacctccaa agccatcgc catggacct 
      ccacttcgg cgacatcgc ctgccagtt caacagcac 
      cgagttctc cctcgccga cctcccagc cacctgcga 
      caccgccaa cagttccga gatctgcct ctccttcaa 
      catcaacgg aactcctta ctgcgaatg gaactacaa 
      cggtctcgg catcgacta ccaaatcct agccgggcc 
      caccttcat ggtcgtctt caccgtcgt gggcgtcat 
      cctcggcat agctgccga caagtacaa ccgggtgcg 
      cattctggc catttgtac catcatttt ctccatcgc 
      cattatcct ctgtggggg cgtgacgga gttctggca 
      gctggtgct gctgaggat gatcatggc ggctgggga 
      gtcggggtg caaccccct ggccacggg cctgctctc 
      ggacatctt ccccgagaa gcagcgcgc gctggccat 
      gtccatctt caactgggg gatttatgg gggctacgg 
      gatcgcctt ccccgtggg gcgctatgt gcccccgct 
      gaacgcctg gggcttggg ctggagagt cacctatta 
      cgggactgg gattgtggc gctgatcgt ggggctgct 
      cacctggtt cacgctgaa ggaaccccc aagacagtc 
      gatcgggga agacgcaga gcatgatcc caatgccaa 
      gaaaatcac catttggac gattattct cgacccgag 
      gatcattat gctgtgttt ggcggcgag tctgaggca 
      ctgtggggg catgtgttt cgcttacaa ctgcgatct 
      gtattacca gcagtactt ccccgagta tgatctggg 
      gtggtggct gtttgctgt cactatcat tgttgggag 
      tattggagt ggtggttgg gggaatcgt ctcagatgt 
      tttcgttgc caaaatggg gataaggtc gagagttat 
      ggtacttgc catcagtca gattattgc cacaccggg 
      agccttcgg cagtgttta tttcaatcc cacgtgggc 
      gatgatttt cttaggact gtcgtactt tttcggtga 
      gtttcgaac aaaataata acactagtt tatattttt 
      ttaccaaga aataattta tattgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY