Status
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Gene information
General information
TC033401
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015596,TC015597
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3223273..3254511
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033401
    iBeetle screen iB_05845

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033401 RA
      MPGIVVFRR RWSVGSDDL VVPGAFLFT VHLIWTVIL 
      TVFLSLATF ENLVHCELQ LLGLVLGYV VILALSMAI 
      ELAICVIAM KGSILDTAP RASMQYILY FRVGKTISL 
      KVLRAYQDS WQQRCRFLF CCSSASDRN RNSFADIAR 
      LLSDFFRDL DVVPSDVIA GLVLLRKFQ KIERKAIVE 
      QRKNDTYEF LSGVAVTPR TQFLSLQEG ADLELFQTV 
      IHFAHFAVR AYGWPIYVM TSSMGLCRL CTDLRCFCC 
      FPCQKPDAA EIVKDNCCK CNYAALQKL TSLGDIEII 
      YATYHVDVG QTPFFVALD YDRKKVVIS IRGTLSMKW 
      KTITCSIVC CGCSQPTSA VELSTGESD VGEYMRSKR 
      TARGGLHPS DSTIALTSH QPLYPPGRI IHVVRHHPT 
      RGEQKYEKR WRQALSKRE PVYQALWAN NTDFDEVLI 
      SPVMIQDHM PDKVLDALN KVRSHHAHQ PDIYMFSDQ 
      VVTTLGPRK PQRSSSGNP QKCWEEDGL LSNLSPTPP 
      HRVYLETSF TSLQSPSGA SSLTNGFYS TRSNPSPIF 
      PITKSLSLT LKRQTPSPA LQLDLIHDD WFGLAPLAS 
      PESLSEVSS ISSVLVTQT TVCEINTPK IMRRTPKII 
      GSLSTCADD IRNVRNFQK AEVFLRCGN GSPATNSSS 
      NLSYESATN EPELKCCES DEFQSAEDI LDNVLASAG 
      CRDFFNNNE GFLETHFDL FEENAKTSK FVQSPSTPQ 
      DMDPDIISN FAIDLNLDS EHRSLQFVT RSTETSKPN 
      SVDSGSVCR TPSDTKNVT FNPQVINID STLVSTAYS 
      SPRRHWHLF KGRKHKSGA ETRVTSPDT SPPVPKTIL 
      CVKRRNSEE ECFSRTEAL PLLSGLGVN SERTSPNFV 
      RRKKYVYPV EMTIKGESS V
    • >TC033401 RB
      MPGIVVFRR RWSVGSDDL VVPGAFLFT VHLIWTVIL 
      TVFLSLATF ENLVHCELQ LLGLVLGYV VILALSMAI 
      ELAICVIAM KGSILDTAP RASMQYILY FRVGKTISL 
      KVLRAYQDS WQQRCRFLF CCSSASDRN RNSFADIAR 
      LLSDFFRDL DVVPSDVIA GLVLLRKFQ KIERKAIVE 
      QRKNDTYEF LSGVAVTPR TQFLSLQEG ADLELFQTV 
      IHFAHFAVR AYGWPIYVM TSSMGLCRL CTDLRCFCC 
      FPCQKPDAA EIVKDNCCK CNYAALQKL TSLGDIEII 
      YATYHVDVG QTPFFVALD YDRKKVVIS IRGTLSMKW 
      KTITCSIVC CGCSQPTSA VELSTGESD VGEYMRSKR 
      TARGGLHPS DSTIALTSH QPLYPPGRI IHVVRHHPT 
      RGEQKYEKR WRQALSKRE PVYQALWAN NTDFDEVLI 
      SPVMIQDHM PDKVLDALN KVRSHHAHQ PDIYMFSDQ 
      VVTTLGPRK PQRSSSGNP QKCWEEDGL LSNLSPTPP 
      HRVYLETSF TSLQSPSGA SSLTNGFYS TRSNPSPIF 
      PITKSLSLT LKRQTPSPA LQLDLIHDD WFGLAPLAS 
      PESLSEVSS ISSVLVTQT TVCEINTPK IMRRTPKII 
      GSLSTCADD IRNVRNFQK AEVFLRCGN GSPATNSSS 
      NLSYESATN EPELKCCES DEFQSAEDI LDNVLASAG 
      CRDFFNNNE GFLETHFDL FEENAKTSK FVQSPSTPQ 
      DMDPNIISN FAIDLNLDE EHRSFQFDM DPDIISNFA 
      IDLNLDSEH RSLQFVTRS TETSKPNSV DSGSVCRTP 
      SDTKNVTFN PQVINIDST LVSTAYSSP RRHWHLFKG 
      RKHKSGAET RVTSPDTSP PVPKTILCV KRRNSEEEC 
      FSRTEALPL LSGLGVNSE RTSPNFVRR KKYVYPVEM 
      TIKGESSV
    CDS sequence
    • >TC033401 RA
      atgccagga attgttgtt tttcggaga cgatggagt 
      gttggatcg gacgatttg gtcgtgcct ggggctttt 
      ctcttcacc gtccacctg atttggact gtcatcttg 
      acggtattt ctcagtttg gccacattt gaaaatcta 
      gtacactgt gaattgcaa ctgctgggt ttggttctg 
      ggctatgtg gtaattcta gcactgtct atggccatc 
      gaactagcc atctgtgtg atagccatg aaggggagc 
      attttggac acagcacca cgtgcttcc atgcagtat 
      attctttat tttagagta ggtaagaca attagctta 
      aaagtcctg cgcgcctat caggacagc tggcagcaa 
      cggtgccgt ttcctcttc tgttgttct tcagcctcg 
      gaccgcaac cgcaactca ttcgccgac atcgctcgc 
      ctcctttcc gacttcttc cgcgacttg gacgtcgtc 
      ccatccgac gtaatcgcc ggcttagtc cttctccgc 
      aaattccag aaaatcgaa cgcaaggcc atcgtagag 
      cagcgcaaa aacgacacc tatgagttc ctttccggc 
      gtcgctgtg actccccga acacagttt ctctcgctg 
      caggaaggt gccgacctg gaactcttc cagactgtt 
      attcatttt gctcatttt gccgtccgc gcttacggt 
      tggcccatt tatgtcatg acgtcgtcg atgggactt 
      tgtcggctg tgcaccgat ttgcgatgc ttttgctgt 
      tttccgtgc caaaaacca gacgcggcc gaaattgtt 
      aaggataat tgctgtaaa tgtaattat gcggcgttg 
      cagaaactt accagcttg ggggacatt gagattatt 
      tatgcgact tatcatgtg gatgtggga cagacgccg 
      ttctttgtg gcgttggat tatgatagg aaaaaggtt 
      gtgattagt attaggggc actttgagt atgaagtgg 
      aagaccatc acgtgtagt attgtttgc tgtggctgc 
      tcccagccg acgtccgct gtggagttg tccactggt 
      gaaagcgat gtgggggag tatatgagg tcgaaacgc 
      acggccaga ggaggtttg catccttct gatagtacc 
      atagctttg acttcgcat caaccactg tatcctcct 
      ggacgaata atccacgtg gtgagacat catccaaca 
      cgtggcgaa caaaagtat gaaaaaaga tggaggcaa 
      gcattgtca aagcgcgaa ccggtctat caagcactt 
      tgggcaaac aacaccgat ttcgatgaa gtattaata 
      tctccagtg atgatacag gaccacatg cctgataaa 
      gtattagat gccctcaac aaggtacgc tcgcatcac 
      gcgcatcag cccgatatc tacatgttc agtgaccag 
      gttgtaaca accctcggc ccccgaaaa ccgcaacgc 
      tcctcctcc ggcaacccc cagaagtgc tgggaggag 
      gacggtctt ctctccaac ctcagtccc acgcctcca 
      caccgcgtg tacctggag accagcttc acctccttg 
      cagagtccc agtggcgcg tcttcactc accaacggc 
      ttctactcc accaggagc aacccctcc cccatcttc 
      cccatcacc aaaagcctc agtctgact ctcaagcgc 
      caaacacca agtccagcc ctgcagctc gacctcatc 
      cacgacgac tggttcgga ctcgcaccg ctggcaagt 
      cccgagagc ctgtcggag gtgtcgagc atcagcagt 
      gtcctggtc acccagacg accgtctgc gagatcaac 
      acgcccaag atcatgaga cggacgccg aaaatcatc 
      ggcagtctg agcacatgt gctgatgat atccggaat 
      gtgaggaac ttccaaaag gcggaggtt ttcctgagg 
      tgtgggaac ggcagtccg gccacaaac tccagcagt 
      aatttgagc tatgagtcg gcgacgaac gagccggag 
      ttgaaatgc tgtgaaagt gacgagttt caatcagct 
      gaggatatc ctggataat gttctagcc agtgccggg 
      tgcagggat ttttttaac aataatgag gggtttttg 
      gaaacgcat tttgacttg tttgaagaa aatgccaag 
      acgagcaaa tttgtccag tctccgtcc acaccacag 
      gacatggat cccgacata atctccaac ttcgccatc 
      gacctcaac ttggactct gagcaccgt tccctccag 
      ttcgtcacc cgaagcacg gaaacctcc aagccgaac 
      agtgtcgac tctggtagc gtttgtcgc accccatcc 
      gacacgaaa aacgtgaca ttcaacccc caggtcatc 
      aatatcgac agcacgttg gtttcgacc gcctacagc 
      tcgcctcgc cgacactgg cacctattc aagggccgc 
      aagcacaaa agtggcgca gaaacgcgt gtcacttcc 
      cccgacacc tcgcccccg gtccccaaa accatcctg 
      tgcgtgaaa agacgcaac agtgaggaa gagtgcttt 
      tcccggacc gaggctttg ccgctgttg agcggactc 
      ggcgttaac agtgaacgg acgtctccg aatttcgtc 
      agacggaag aagtatgtc tatccggtt gaaatgacg 
      atcaaaggg gagagtagt gtgtag
    • >TC033401 RB
      atgccagga attgttgtt tttcggaga cgatggagt 
      gttggatcg gacgatttg gtcgtgcct ggggctttt 
      ctcttcacc gtccacctg atttggact gtcatcttg 
      acggtattt ctcagtttg gccacattt gaaaatcta 
      gtacactgt gaattgcaa ctgctgggt ttggttctg 
      ggctatgtg gtaattcta gcactgtct atggccatc 
      gaactagcc atctgtgtg atagccatg aaggggagc 
      attttggac acagcacca cgtgcttcc atgcagtat 
      attctttat tttagagta ggtaagaca attagctta 
      aaagtcctg cgcgcctat caggacagc tggcagcaa 
      cggtgccgt ttcctcttc tgttgttct tcagcctcg 
      gaccgcaac cgcaactca ttcgccgac atcgctcgc 
      ctcctttcc gacttcttc cgcgacttg gacgtcgtc 
      ccatccgac gtaatcgcc ggcttagtc cttctccgc 
      aaattccag aaaatcgaa cgcaaggcc atcgtagag 
      cagcgcaaa aacgacacc tatgagttc ctttccggc 
      gtcgctgtg actccccga acacagttt ctctcgctg 
      caggaaggt gccgacctg gaactcttc cagactgtt 
      attcatttt gctcatttt gccgtccgc gcttacggt 
      tggcccatt tatgtcatg acgtcgtcg atgggactt 
      tgtcggctg tgcaccgat ttgcgatgc ttttgctgt 
      tttccgtgc caaaaacca gacgcggcc gaaattgtt 
      aaggataat tgctgtaaa tgtaattat gcggcgttg 
      cagaaactt accagcttg ggggacatt gagattatt 
      tatgcgact tatcatgtg gatgtggga cagacgccg 
      ttctttgtg gcgttggat tatgatagg aaaaaggtt 
      gtgattagt attaggggc actttgagt atgaagtgg 
      aagaccatc acgtgtagt attgtttgc tgtggctgc 
      tcccagccg acgtccgct gtggagttg tccactggt 
      gaaagcgat gtgggggag tatatgagg tcgaaacgc 
      acggccaga ggaggtttg catccttct gatagtacc 
      atagctttg acttcgcat caaccactg tatcctcct 
      ggacgaata atccacgtg gtgagacat catccaaca 
      cgtggcgaa caaaagtat gaaaaaaga tggaggcaa 
      gcattgtca aagcgcgaa ccggtctat caagcactt 
      tgggcaaac aacaccgat ttcgatgaa gtattaata 
      tctccagtg atgatacag gaccacatg cctgataaa 
      gtattagat gccctcaac aaggtacgc tcgcatcac 
      gcgcatcag cccgatatc tacatgttc agtgaccag 
      gttgtaaca accctcggc ccccgaaaa ccgcaacgc 
      tcctcctcc ggcaacccc cagaagtgc tgggaggag 
      gacggtctt ctctccaac ctcagtccc acgcctcca 
      caccgcgtg tacctggag accagcttc acctccttg 
      cagagtccc agtggcgcg tcttcactc accaacggc 
      ttctactcc accaggagc aacccctcc cccatcttc 
      cccatcacc aaaagcctc agtctgact ctcaagcgc 
      caaacacca agtccagcc ctgcagctc gacctcatc 
      cacgacgac tggttcgga ctcgcaccg ctggcaagt 
      cccgagagc ctgtcggag gtgtcgagc atcagcagt 
      gtcctggtc acccagacg accgtctgc gagatcaac 
      acgcccaag atcatgaga cggacgccg aaaatcatc 
      ggcagtctg agcacatgt gctgatgat atccggaat 
      gtgaggaac ttccaaaag gcggaggtt ttcctgagg 
      tgtgggaac ggcagtccg gccacaaac tccagcagt 
      aatttgagc tatgagtcg gcgacgaac gagccggag 
      ttgaaatgc tgtgaaagt gacgagttt caatcagct 
      gaggatatc ctggataat gttctagcc agtgccggg 
      tgcagggat ttttttaac aataatgag gggtttttg 
      gaaacgcat tttgacttg tttgaagaa aatgccaag 
      acgagcaaa tttgtccag tctccgtcc acaccacag 
      gacatggat cccaacata atctccaac ttcgccatc 
      gacctcaac ttggacgaa gagcaccgt tccttccag 
      ttcgacatg gatcccgac ataatctcc aacttcgcc 
      atcgacctc aacttggac tctgagcac cgttccctc 
      cagttcgtc acccgaagc acggaaacc tccaagccg 
      aacagtgtc gactctggt agcgtttgt cgcacccca 
      tccgacacg aaaaacgtg acattcaac ccccaggtc 
      atcaatatc gacagcacg ttggtttcg accgcctac 
      agctcgcct cgccgacac tggcaccta ttcaagggc 
      cgcaagcac aaaagtggc gcagaaacg cgtgtcact 
      tcccccgac acctcgccc ccggtcccc aaaaccatc 
      ctgtgcgtg aaaagacgc aacagtgag gaagagtgc 
      ttttcccgg accgaggct ttgccgctg ttgagcgga 
      ctcggcgtt aacagtgaa cggacgtct ccgaatttc 
      gtcagacgg aagaagtat gtctatccg gttgaaatg 
      acgatcaaa ggggagagt agtgtgtag
    mRNA sequence
    • >TC033401 RA
      cctaataaa cccgtcaga accgaaatg aagagatct 
      ctctaccga tttatccac ttcggtggt aggtatatc 
      tgggccgtg catatcggg ggcgtcgtg gcgcctgcg 
      tcgcgcacc ccgtgaaaa taccgacgc caacaaaga 
      attcccttt ccttgtcct ccccgaaag cgctctaat 
      cccttcttc attcctcgt ccttgtcac actccaagc 
      agacgcctt gctaacacc gacgtctta gtgcgtaaa 
      cgacggccg gaatggtaa cttgatcta aaacccgat 
      cgtggacca tatacaagg tgttggcaa aaaaatcag 
      cgagagtga gagtgctga tgatttttg tggtctaga 
      agtgtgtgg aaggccaac gaaatagcc attcaatgg 
      gtgctgtga ggacctcaa agaggacaa attcaaggt 
      aacaccacg tctggtcac cagtaacaa actttcctc 
      aacccgagc cacgaaact tcctattga taaagaaaa 
      aaatgtgag tgtgatgct gtgttctcg tacaatgcc 
      cgagataat gacagacgc agcggctcc tagaagttg 
      ttcttcccc cagtgttat gtagcagtg aatgacgag 
      ttagtggtt gtggactgt gttttttta tgtgagaat 
      atgccagga attgttgtt tttcggaga cgatggagt 
      gttggatcg gacgatttg gtcgtgcct ggggctttt 
      ctcttcacc gtccacctg atttggact gtcatcttg 
      acggtattt ctcagtttg gccacattt gaaaatcta 
      gtacactgt gaattgcaa ctgctgggt ttggttctg 
      ggctatgtg gtaattcta gcactgtct atggccatc 
      gaactagcc atctgtgtg atagccatg aaggggagc 
      attttggac acagcacca cgtgcttcc atgcagtat 
      attctttat tttagagta ggtaagaca attagctta 
      aaagtcctg cgcgcctat caggacagc tggcagcaa 
      cggtgccgt ttcctcttc tgttgttct tcagcctcg 
      gaccgcaac cgcaactca ttcgccgac atcgctcgc 
      ctcctttcc gacttcttc cgcgacttg gacgtcgtc 
      ccatccgac gtaatcgcc ggcttagtc cttctccgc 
      aaattccag aaaatcgaa cgcaaggcc atcgtagag 
      cagcgcaaa aacgacacc tatgagttc ctttccggc 
      gtcgctgtg actccccga acacagttt ctctcgctg 
      caggaaggt gccgacctg gaactcttc cagactgtt 
      attcatttt gctcatttt gccgtccgc gcttacggt 
      tggcccatt tatgtcatg acgtcgtcg atgggactt 
      tgtcggctg tgcaccgat ttgcgatgc ttttgctgt 
      tttccgtgc caaaaacca gacgcggcc gaaattgtt 
      aaggataat tgctgtaaa tgtaattat gcggcgttg 
      cagaaactt accagcttg ggggacatt gagattatt 
      tatgcgact tatcatgtg gatgtggga cagacgccg 
      ttctttgtg gcgttggat tatgatagg aaaaaggtt 
      gtgattagt attaggggc actttgagt atgaagtgg 
      aagaccatc acgtgtagt attgtttgc tgtggctgc 
      tcccagccg acgtccgct gtggagttg tccactggt 
      gaaagcgat gtgggggag tatatgagg tcgaaacgc 
      acggccaga ggaggtttg catccttct gatagtacc 
      atagctttg acttcgcat caaccactg tatcctcct 
      ggacgaata atccacgtg gtgagacat catccaaca 
      cgtggcgaa caaaagtat gaaaaaaga tggaggcaa 
      gcattgtca aagcgcgaa ccggtctat caagcactt 
      tgggcaaac aacaccgat ttcgatgaa gtattaata 
      tctccagtg atgatacag gaccacatg cctgataaa 
      gtattagat gccctcaac aaggtacgc tcgcatcac 
      gcgcatcag cccgatatc tacatgttc agtgaccag 
      gttgtaaca accctcggc ccccgaaaa ccgcaacgc 
      tcctcctcc ggcaacccc cagaagtgc tgggaggag 
      gacggtctt ctctccaac ctcagtccc acgcctcca 
      caccgcgtg tacctggag accagcttc acctccttg 
      cagagtccc agtggcgcg tcttcactc accaacggc 
      ttctactcc accaggagc aacccctcc cccatcttc 
      cccatcacc aaaagcctc agtctgact ctcaagcgc 
      caaacacca agtccagcc ctgcagctc gacctcatc 
      cacgacgac tggttcgga ctcgcaccg ctggcaagt 
      cccgagagc ctgtcggag gtgtcgagc atcagcagt 
      gtcctggtc acccagacg accgtctgc gagatcaac 
      acgcccaag atcatgaga cggacgccg aaaatcatc 
      ggcagtctg agcacatgt gctgatgat atccggaat 
      gtgaggaac ttccaaaag gcggaggtt ttcctgagg 
      tgtgggaac ggcagtccg gccacaaac tccagcagt 
      aatttgagc tatgagtcg gcgacgaac gagccggag 
      ttgaaatgc tgtgaaagt gacgagttt caatcagct 
      gaggatatc ctggataat gttctagcc agtgccggg 
      tgcagggat ttttttaac aataatgag gggtttttg 
      gaaacgcat tttgacttg tttgaagaa aatgccaag 
      acgagcaaa tttgtccag tctccgtcc acaccacag 
      gacatggat cccgacata atctccaac ttcgccatc 
      gacctcaac ttggactct gagcaccgt tccctccag 
      ttcgtcacc cgaagcacg gaaacctcc aagccgaac 
      agtgtcgac tctggtagc gtttgtcgc accccatcc 
      gacacgaaa aacgtgaca ttcaacccc caggtcatc 
      aatatcgac agcacgttg gtttcgacc gcctacagc 
      tcgcctcgc cgacactgg cacctattc aagggccgc 
      aagcacaaa agtggcgca gaaacgcgt gtcacttcc 
      cccgacacc tcgcccccg gtccccaaa accatcctg 
      tgcgtgaaa agacgcaac agtgaggaa gagtgcttt 
      tcccggacc gaggctttg ccgctgttg agcggactc 
      ggcgttaac agtgaacgg acgtctccg aatttcgtc 
      agacggaag aagtatgtc tatccggtt gaaatgacg 
      atcaaaggg gagagtagt gtgtagtgc ctctgtacc 
      cgttttctg gtgttctta cttaggcca ggattctta 
      aggtaagag ttttggtct aacttcccg accaaaccg 
      attttccaa acgcctttg aggattgat ttttgttca 
      gacgaagcc taatgtctg tgaacacat gtattcata 
      cccgggaac tagtagggt ccaagaatg tacagtaga 
      ctacaaaat gtacaagat gtgtattta attattaaa 
      tttaaatat aacgttaca attttc
    • >TC033401 RB
      cctaataaa cccgtcaga accgaaatg aagagatct 
      ctctaccga tttatccac ttcggtggt aggtatatc 
      tgggccgtg catatcggg ggcgtcgtg gcgcctgcg 
      tcgcgcacc ccgtgaaaa taccgacgc caacaaaga 
      attcccttt ccttgtcct ccccgaaag cgctctaat 
      cccttcttc attcctcgt ccttgtcac actccaagc 
      agacgcctt gctaacacc gacgtctta gtgcgtaaa 
      cgacggccg gaatggtaa cttgatcta aaacccgat 
      cgtggacca tatacaagg tgttggcaa aaaaatcag 
      cgagagtga gagtgctga tgatttttg tggtctaga 
      agtgtgtgg aaggccaac gaaatagcc attcaatgg 
      gtgctgtga ggacctcaa agaggacaa attcaaggt 
      aacaccacg tctggtcac cagtaacaa actttcctc 
      aacccgagc cacgaaact tcctattga taaagaaaa 
      aaatgtgag tgtgatgct gtgttctcg tacaatgcc 
      cgagataat gacagacgc agcggctcc tagaagttg 
      ttcttcccc cagtgttat gtagcagtg aatgacgag 
      ttagtggtt gtggactgt gttttttta tgtgagaat 
      atgccagga attgttgtt tttcggaga cgatggagt 
      gttggatcg gacgatttg gtcgtgcct ggggctttt 
      ctcttcacc gtccacctg atttggact gtcatcttg 
      acggtattt ctcagtttg gccacattt gaaaatcta 
      gtacactgt gaattgcaa ctgctgggt ttggttctg 
      ggctatgtg gtaattcta gcactgtct atggccatc 
      gaactagcc atctgtgtg atagccatg aaggggagc 
      attttggac acagcacca cgtgcttcc atgcagtat 
      attctttat tttagagta ggtaagaca attagctta 
      aaagtcctg cgcgcctat caggacagc tggcagcaa 
      cggtgccgt ttcctcttc tgttgttct tcagcctcg 
      gaccgcaac cgcaactca ttcgccgac atcgctcgc 
      ctcctttcc gacttcttc cgcgacttg gacgtcgtc 
      ccatccgac gtaatcgcc ggcttagtc cttctccgc 
      aaattccag aaaatcgaa cgcaaggcc atcgtagag 
      cagcgcaaa aacgacacc tatgagttc ctttccggc 
      gtcgctgtg actccccga acacagttt ctctcgctg 
      caggaaggt gccgacctg gaactcttc cagactgtt 
      attcatttt gctcatttt gccgtccgc gcttacggt 
      tggcccatt tatgtcatg acgtcgtcg atgggactt 
      tgtcggctg tgcaccgat ttgcgatgc ttttgctgt 
      tttccgtgc caaaaacca gacgcggcc gaaattgtt 
      aaggataat tgctgtaaa tgtaattat gcggcgttg 
      cagaaactt accagcttg ggggacatt gagattatt 
      tatgcgact tatcatgtg gatgtggga cagacgccg 
      ttctttgtg gcgttggat tatgatagg aaaaaggtt 
      gtgattagt attaggggc actttgagt atgaagtgg 
      aagaccatc acgtgtagt attgtttgc tgtggctgc 
      tcccagccg acgtccgct gtggagttg tccactggt 
      gaaagcgat gtgggggag tatatgagg tcgaaacgc 
      acggccaga ggaggtttg catccttct gatagtacc 
      atagctttg acttcgcat caaccactg tatcctcct 
      ggacgaata atccacgtg gtgagacat catccaaca 
      cgtggcgaa caaaagtat gaaaaaaga tggaggcaa 
      gcattgtca aagcgcgaa ccggtctat caagcactt 
      tgggcaaac aacaccgat ttcgatgaa gtattaata 
      tctccagtg atgatacag gaccacatg cctgataaa 
      gtattagat gccctcaac aaggtacgc tcgcatcac 
      gcgcatcag cccgatatc tacatgttc agtgaccag 
      gttgtaaca accctcggc ccccgaaaa ccgcaacgc 
      tcctcctcc ggcaacccc cagaagtgc tgggaggag 
      gacggtctt ctctccaac ctcagtccc acgcctcca 
      caccgcgtg tacctggag accagcttc acctccttg 
      cagagtccc agtggcgcg tcttcactc accaacggc 
      ttctactcc accaggagc aacccctcc cccatcttc 
      cccatcacc aaaagcctc agtctgact ctcaagcgc 
      caaacacca agtccagcc ctgcagctc gacctcatc 
      cacgacgac tggttcgga ctcgcaccg ctggcaagt 
      cccgagagc ctgtcggag gtgtcgagc atcagcagt 
      gtcctggtc acccagacg accgtctgc gagatcaac 
      acgcccaag atcatgaga cggacgccg aaaatcatc 
      ggcagtctg agcacatgt gctgatgat atccggaat 
      gtgaggaac ttccaaaag gcggaggtt ttcctgagg 
      tgtgggaac ggcagtccg gccacaaac tccagcagt 
      aatttgagc tatgagtcg gcgacgaac gagccggag 
      ttgaaatgc tgtgaaagt gacgagttt caatcagct 
      gaggatatc ctggataat gttctagcc agtgccggg 
      tgcagggat ttttttaac aataatgag gggtttttg 
      gaaacgcat tttgacttg tttgaagaa aatgccaag 
      acgagcaaa tttgtccag tctccgtcc acaccacag 
      gacatggat cccaacata atctccaac ttcgccatc 
      gacctcaac ttggacgaa gagcaccgt tccttccag 
      ttcgacatg gatcccgac ataatctcc aacttcgcc 
      atcgacctc aacttggac tctgagcac cgttccctc 
      cagttcgtc acccgaagc acggaaacc tccaagccg 
      aacagtgtc gactctggt agcgtttgt cgcacccca 
      tccgacacg aaaaacgtg acattcaac ccccaggtc 
      atcaatatc gacagcacg ttggtttcg accgcctac 
      agctcgcct cgccgacac tggcaccta ttcaagggc 
      cgcaagcac aaaagtggc gcagaaacg cgtgtcact 
      tcccccgac acctcgccc ccggtcccc aaaaccatc 
      ctgtgcgtg aaaagacgc aacagtgag gaagagtgc 
      ttttcccgg accgaggct ttgccgctg ttgagcgga 
      ctcggcgtt aacagtgaa cggacgtct ccgaatttc 
      gtcagacgg aagaagtat gtctatccg gttgaaatg 
      acgatcaaa ggggagagt agtgtgtag tgcctctgt 
      acccgtttt ctggtgttc ttacttagg ccaggattc 
      ttaaggtaa gagttttgg tctaacttc ccgaccaaa 
      ccgattttc caaacgcct ttgaggatt gatttttgt 
      tcagacgaa gcctaatgt ctgtgaaca catgtattc 
      atacccggg aactagtag ggtccaaga atgtacagt 
      agactacaa aatgtacaa gatgtgtat ttaattatt 
      aaatttaaa tataacgtt acaattttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY