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Gene information
General information
TC015546 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2144310..2145919
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015546
    iBeetle screen iB_05838

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015546 RA
      MNTTIEILT IIILTIFCF YKWKFSYWK SRKIPYLDP 
      KFPFGNVSN PFFMKEKVG MTLARFYND MKQRGWKYG 
      GFYFGVSPI CLIVDPDLI KNIVTRDFE HFVDRGVYF 
      NEKDDPLSG HLANLDGQK WKNMRRKLN PTFSASKTK 
      NMFPIVKFC VENMMEKVK SCYENAHKC CNINEVFPA 
      FSINVICSC AFGLQEENA LFKAYADEC VSWTKYKYL 
      VQILATFFD KTAKRMGIT TVPRNAAKF FMKVVTDSV 
      KYREENDYV RSDFMQLLI EMKKQNQLT IEEIAAQCF 
      VFFIAGFET SSATLTFAL YELAKNQNL QNVARDEIL 
      ANCPNEITY DSLADLKFL DQVVDETLR KYPPLLYVT 
      RQCVKNYKI PDEDVVIEK GTLVAIPIS ALHSDEEFY 
      PKPETFEPE RFSKTEKSL RHPYTFLPF GEGPRMCIG 
      KRFGLLEVK LALTCLLKN YKFSVNCKT KEPLAMRVD 
      TVVLAPKDE IWLDLEKI
    CDS sequence
    • >TC015546 RA
      atgaacacc accatcgaa attctaaca atcatcatt 
      ttgacaatt ttctgcttc tacaaatgg aaattcagt 
      tactggaaa agccggaaa atcccatac ctcgacccg 
      aaatttcca ttcggaaac gtaagtaac ccgtttttc 
      atgaaggaa aaagtcggc atgactttg gcccgcttt 
      tacaacgac atgaaacaa agagggtgg aaatacggg 
      gggttttat ttcggggtt tcccccatc tgtctaatc 
      gtcgatcct gacctcatc aaaaatata gtcacgcgt 
      gattttgag cattttgtc gaccgtggt gtgtatttt 
      aacgaaaaa gacgaccct ttgagcgga caccttgcc 
      aatctagac ggccagaag tggaaaaac atgcgcaga 
      aaactaaac ccgactttc tcggcgtct aagacgaaa 
      aacatgttc ccgattgta aaattctgt gtggaaaat 
      atgatggaa aaagtgaaa agttgttac gaaaacgcc 
      cacaagtgt tgtaacatc aacgaggtc ttccccgcg 
      ttttccatc aacgtgatc tgttcgtgc gccttcggc 
      ttgcaggag gaaaacgcg cttttcaaa gcctacgct 
      gacgagtgc gtcagttgg acaaagtac aagtacttg 
      gtccagatt ttggccacg tttttcgac aaaacggcc 
      aaaagaatg ggaatcacg acagtgcca cgaaatgcg 
      gccaagttt ttcatgaaa gtggtaaca gacagtgtc 
      aaatatcgg gaggaaaac gattacgtc cggagtgat 
      tttatgcaa ttgttgatc gaaatgaaa aagcaaaac 
      caactgact attgaggaa atcgccgct caatgtttc 
      gtgtttttc atcgccggt ttcgaaacg tcttcggcc 
      actctaacg ttcgcttta tacgaactt gcaaaaaac 
      caaaacttg caaaatgtg gcacgtgac gagattttg 
      gccaattgt cccaacgaa atcacgtac gactcgctt 
      gccgacttg aaattttta gaccaagtg gttgatgaa 
      actttacgc aaatacccg ccactgttg tacgtgacc 
      agacagtgt gttaaaaat tataagatc ccagacgag 
      gatgttgtt atcgaaaaa gggactttg gtggcgatc 
      cccatttcg gcgctgcat tccgatgag gagttttat 
      cccaagccg gaaacgttc gaaccggag cgatttagc 
      aaaactgag aaaagtttg agacatcct tatacgttt 
      ctgccgttt ggcgaaggg ccaagaatg tgtattggt 
      aaacggttc ggtttgttg gaagttaag ttagcgctg 
      acttgttta ctgaaaaat tacaaattt tcggtcaat 
      tgcaaaact aaggagccg ttagccatg agagtggat 
      acggttgtt ttggcgccc aaagatgag atttggtta 
      gacttagaa aaaatataa
    mRNA sequence
    • >TC015546 RA
      cccacacaa ataatccaa caatcgcca tgaacacca 
      ccatcgaaa ttctaacaa tcatcattt tgacaattt 
      tctgcttct acaaatgga aattcagtt actggaaaa 
      gccggaaaa tcccatacc tcgacccga aatttccat 
      tcggaaacg taagtaacc cgtttttca tgaaggaaa 
      aagtcggca tgactttgg cccgctttt acaacgaca 
      tgaaacaaa gagggtgga aatacgggg ggttttatt 
      tcggggttt cccccatct gtctaatcg tcgatcctg 
      acctcatca aaaatatag tcacgcgtg attttgagc 
      attttgtcg accgtggtg tgtatttta acgaaaaag 
      acgaccctt tgagcggac accttgcca atctagacg 
      gccagaagt ggaaaaaca tgcgcagaa aactaaacc 
      cgactttct cggcgtcta agacgaaaa acatgttcc 
      cgattgtaa aattctgtg tggaaaata tgatggaaa 
      aagtgaaaa gttgttacg aaaacgccc acaagtgtt 
      gtaacatca acgaggtct tccccgcgt tttccatca 
      acgtgatct gttcgtgcg ccttcggct tgcaggagg 
      aaaacgcgc ttttcaaag cctacgctg acgagtgcg 
      tcagttgga caaagtaca agtacttgg tccagattt 
      tggccacgt ttttcgaca aaacggcca aaagaatgg 
      gaatcacga cagtgccac gaaatgcgg ccaagtttt 
      tcatgaaag tggtaacag acagtgtca aatatcggg 
      aggaaaacg attacgtcc ggagtgatt ttatgcaat 
      tgttgatcg aaatgaaaa agcaaaacc aactgacta 
      ttgaggaaa tcgccgctc aatgtttcg tgtttttca 
      tcgccggtt tcgaaacgt cttcggcca ctctaacgt 
      tcgctttat acgaacttg caaaaaacc aaaacttgc 
      aaaatgtgg cacgtgacg agattttgg ccaattgtc 
      ccaacgaaa tcacgtacg actcgcttg ccgacttga 
      aatttttag accaagtgg ttgatgaaa ctttacgca 
      aatacccgc cactgttgt acgtgacca gacagtgtg 
      ttaaaaatt ataagatcc cagacgagg atgttgtta 
      tcgaaaaag ggactttgg tggcgatcc ccatttcgg 
      cgctgcatt ccgatgagg agttttatc ccaagccgg 
      aaacgttcg aaccggagc gatttagca aaactgaga 
      aaagtttga gacatcctt atacgtttc tgccgtttg 
      gcgaagggc caagaatgt gtattggta aacggttcg 
      gtttgttgg aagttaagt tagcgctga cttgtttac 
      tgaaaaatt acaaatttt cggtcaatt gcaaaacta 
      aggagccgt tagccatga gagtggata cggttgttt 
      tggcgccca aagatgaga tttggttag acttagaaa 
      aaatataat aaaattcaa ttaaaaaaa agtacgttg 
      t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY