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Gene information
General information
TC015546 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2144310..2145919
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015546
    iBeetle screen iB_05838

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015546 RA
      MNTTIEILT IIILTIFCF YKWKFSYWK SRKIPYLDP 
KFPFGNVSN PFFMKEKVG MTLARFYND MKQRGWKYG 
GFYFGVSPI CLIVDPDLI KNIVTRDFE HFVDRGVYF 
NEKDDPLSG HLANLDGQK WKNMRRKLN PTFSASKTK 
NMFPIVKFC VENMMEKVK SCYENAHKC CNINEVFPA 
FSINVICSC AFGLQEENA LFKAYADEC VSWTKYKYL 
VQILATFFD KTAKRMGIT TVPRNAAKF FMKVVTDSV 
KYREENDYV RSDFMQLLI EMKKQNQLT IEEIAAQCF 
VFFIAGFET SSATLTFAL YELAKNQNL QNVARDEIL 
ANCPNEITY DSLADLKFL DQVVDETLR KYPPLLYVT 
RQCVKNYKI PDEDVVIEK GTLVAIPIS ALHSDEEFY 
PKPETFEPE RFSKTEKSL RHPYTFLPF GEGPRMCIG 
KRFGLLEVK LALTCLLKN YKFSVNCKT KEPLAMRVD 
TVVLAPKDE IWLDLEKI
    CDS sequence
    • >TC015546 RA
      atgaacacc accatcgaa attctaaca atcatcatt 
ttgacaatt ttctgcttc tacaaatgg aaattcagt 
tactggaaa agccggaaa atcccatac ctcgacccg 
aaatttcca ttcggaaac gtaagtaac ccgtttttc 
atgaaggaa aaagtcggc atgactttg gcccgcttt 
tacaacgac atgaaacaa agagggtgg aaatacggg 
gggttttat ttcggggtt tcccccatc tgtctaatc 
gtcgatcct gacctcatc aaaaatata gtcacgcgt 
gattttgag cattttgtc gaccgtggt gtgtatttt 
aacgaaaaa gacgaccct ttgagcgga caccttgcc 
aatctagac ggccagaag tggaaaaac atgcgcaga 
aaactaaac ccgactttc tcggcgtct aagacgaaa 
aacatgttc ccgattgta aaattctgt gtggaaaat 
atgatggaa aaagtgaaa agttgttac gaaaacgcc 
cacaagtgt tgtaacatc aacgaggtc ttccccgcg 
ttttccatc aacgtgatc tgttcgtgc gccttcggc 
ttgcaggag gaaaacgcg cttttcaaa gcctacgct 
gacgagtgc gtcagttgg acaaagtac aagtacttg 
gtccagatt ttggccacg tttttcgac aaaacggcc 
aaaagaatg ggaatcacg acagtgcca cgaaatgcg 
gccaagttt ttcatgaaa gtggtaaca gacagtgtc 
aaatatcgg gaggaaaac gattacgtc cggagtgat 
tttatgcaa ttgttgatc gaaatgaaa aagcaaaac 
caactgact attgaggaa atcgccgct caatgtttc 
gtgtttttc atcgccggt ttcgaaacg tcttcggcc 
actctaacg ttcgcttta tacgaactt gcaaaaaac 
caaaacttg caaaatgtg gcacgtgac gagattttg 
gccaattgt cccaacgaa atcacgtac gactcgctt 
gccgacttg aaattttta gaccaagtg gttgatgaa 
actttacgc aaatacccg ccactgttg tacgtgacc 
agacagtgt gttaaaaat tataagatc ccagacgag 
gatgttgtt atcgaaaaa gggactttg gtggcgatc 
cccatttcg gcgctgcat tccgatgag gagttttat 
cccaagccg gaaacgttc gaaccggag cgatttagc 
aaaactgag aaaagtttg agacatcct tatacgttt 
ctgccgttt ggcgaaggg ccaagaatg tgtattggt 
aaacggttc ggtttgttg gaagttaag ttagcgctg 
acttgttta ctgaaaaat tacaaattt tcggtcaat 
tgcaaaact aaggagccg ttagccatg agagtggat 
acggttgtt ttggcgccc aaagatgag atttggtta 
gacttagaa aaaatataa
    mRNA sequence
    • >TC015546 RA
      cccacacaa ataatccaa caatcgcca tgaacacca 
ccatcgaaa ttctaacaa tcatcattt tgacaattt 
tctgcttct acaaatgga aattcagtt actggaaaa 
gccggaaaa tcccatacc tcgacccga aatttccat 
tcggaaacg taagtaacc cgtttttca tgaaggaaa 
aagtcggca tgactttgg cccgctttt acaacgaca 
tgaaacaaa gagggtgga aatacgggg ggttttatt 
tcggggttt cccccatct gtctaatcg tcgatcctg 
acctcatca aaaatatag tcacgcgtg attttgagc 
attttgtcg accgtggtg tgtatttta acgaaaaag 
acgaccctt tgagcggac accttgcca atctagacg 
gccagaagt ggaaaaaca tgcgcagaa aactaaacc 
cgactttct cggcgtcta agacgaaaa acatgttcc 
cgattgtaa aattctgtg tggaaaata tgatggaaa 
aagtgaaaa gttgttacg aaaacgccc acaagtgtt 
gtaacatca acgaggtct tccccgcgt tttccatca 
acgtgatct gttcgtgcg ccttcggct tgcaggagg 
aaaacgcgc ttttcaaag cctacgctg acgagtgcg 
tcagttgga caaagtaca agtacttgg tccagattt 
tggccacgt ttttcgaca aaacggcca aaagaatgg 
gaatcacga cagtgccac gaaatgcgg ccaagtttt 
tcatgaaag tggtaacag acagtgtca aatatcggg 
aggaaaacg attacgtcc ggagtgatt ttatgcaat 
tgttgatcg aaatgaaaa agcaaaacc aactgacta 
ttgaggaaa tcgccgctc aatgtttcg tgtttttca 
tcgccggtt tcgaaacgt cttcggcca ctctaacgt 
tcgctttat acgaacttg caaaaaacc aaaacttgc 
aaaatgtgg cacgtgacg agattttgg ccaattgtc 
ccaacgaaa tcacgtacg actcgcttg ccgacttga 
aatttttag accaagtgg ttgatgaaa ctttacgca 
aatacccgc cactgttgt acgtgacca gacagtgtg 
ttaaaaatt ataagatcc cagacgagg atgttgtta 
tcgaaaaag ggactttgg tggcgatcc ccatttcgg 
cgctgcatt ccgatgagg agttttatc ccaagccgg 
aaacgttcg aaccggagc gatttagca aaactgaga 
aaagtttga gacatcctt atacgtttc tgccgtttg 
gcgaagggc caagaatgt gtattggta aacggttcg 
gtttgttgg aagttaagt tagcgctga cttgtttac 
tgaaaaatt acaaatttt cggtcaatt gcaaaacta 
aggagccgt tagccatga gagtggata cggttgttt 
tggcgccca aagatgaga tttggttag acttagaaa 
aaatataat aaaattcaa ttaaaaaaa agtacgttg 
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