Status
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Gene information
General information
TC015542 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2079705..2097175
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015542
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05836

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015542 RA
      MKKSNTDLN GVLENGTAG LSVRRPVYQ LEDLNRDTD 
      YETPHTTFV QDCGRRFRA FRPFRCLYY SVPVLKWLP 
      KYSCKKNIF GDIISGITV AIMHIPQGM AYGLLGNVP 
      PVVGIYMAF FPVLIYFIF GTSRHVSIG TFAIICLMT 
      GKVVNQYSS IEILQNGTV VTTPSPNPE MPLYTNVEV 
      ATTVTFAVA MIQLVMYSL RLGVVSTLL SETLVNGFT 
      CASAFHVVS SQIKDLFGI PIKKRRGNF GFPLTIYDS 
      VLALSRANP YACGMSAVS CVILIINNE VLKPFLAKK 
      TKIPFPIEL LAVVLGTAS SYFFSLDTK YDISVVGHI 
      PTGFPAPTP PAFALIPDI LVDAFVITM VSYTITMSM 
      ALIFARKLF YEVDSNQEL LALGLSNTM GSFFACMPV 
      TASLSRSMI QEAVGGVTQ IASIVSCSI LLVILLWIG 
      PLFETLPRC VLASIIVVA LKGMLFQCQ SIVRYWKLS 
      KWDALVWTV TFCTTLFVQ IGYGLAAGV AVSLLSVFI 
      QGYKPYTCL LGVVPNTDL YLDIKRYKK AQEIQGVKI 
      FRYSGGLSF ASRSAFKEL LNRKIGFDP ASVLRKRAR 
      LEESPSRST TVTEDFDLL TRCVILDFA SLTFVDPSG 
      VDLLRQLQS DYAKLDIKL YIAACSGPV YEKFIICDQ 
      QEGIESKFM IFPTIHDAV LFAQSNVLR TKQI
    CDS sequence
    • >TC015542 RA
      atgaagaaa agcaatacg gacctgaac ggcgttttg 
      gaaaatgga acagccggg ctgagtgtg agacggccg 
      gtttaccaa cttgaagat ttgaacaga gacactgac 
      tatgagaca ccgcacaca acatttgtg caggattgt 
      ggtcgcaga tttcgggcc ttccggccc tttaggtgt 
      ctctactat tcggtgccg gtgctcaaa tggctccca 
      aaatacagt tgcaagaaa aatattttt ggggatatt 
      atcagtggg attacggtg gctatcatg cacataccg 
      cagggcatg gcgtacggc ctcctcggc aacgtgccc 
      cccgtggtc ggcatctac atggccttc ttccccgta 
      cttatctac ttcattttc ggcacatct cgccacgtc 
      tcgatagga accttcgca atcatttgt ctaatgacc 
      ggcaaagtg gtcaaccaa tacagcagc atcgaaatc 
      ctccaaaac ggcaccgtg gtcaccacc ccgtccccc 
      aaccccgaa atgcccctc tacaccaac gtggaagtc 
      gccacaacc gtcaccttc gccgtggct atgatccaa 
      ctcgtcatg tactccctg cgccttggc gtcgtctcg 
      actttactc agcgaaact ctagtgaac ggtttcacg 
      tgtgcttca gccttccac gtggtctcg tcacaaatc 
      aaagatctg ttcgggatt ccgattaaa aaacgaagg 
      ggaaatttc ggcttccct ttgacgatt tacgactcg 
      gttttggcc ctgtcaagg gccaatccg tacgcttgt 
      ggcatgtcg gcagtatcg tgcgttatc cttatcatc 
      aataacgaa gttttgaag cccttttta gccaagaaa 
      accaaaatt ccgttcccg atcgaattg ctggctgtg 
      gttttgggt acggcctcg agttatttc tttagtttg 
      gatacgaag tacgatatt tcggttgtg gggcacata 
      ccgactggg tttccggcc cccacacct ccagctttt 
      gcccttata ccggatatt ttagtcgat gcgtttgta 
      ataaccatg gtttcgtac actataact atgtccatg 
      gcgttgatt tttgcaagg aagctgttc tatgaggtg 
      gactcgaac caggaactg ttggctttg ggactgagc 
      aacaccatg ggctcgttc ttcgcctgc atgcccgtc 
      acagcctcc ctctcccgc tccatgatc caggaggcc 
      gtcggagga gtgacccaa atcgcctcc atcgtctcc 
      tgcagcatt ctcttggtc atcctcctc tggatcggg 
      ccactcttc gaaaccctc ccccggtgt gtcctagct 
      agcatcatc gtggtggcc ctcaaaggc atgctcttc 
      caatgccaa tcgatcgta aggtattgg aaattgagt 
      aaatgggac gctttggtc tggacggtt actttctgt 
      acaactttg ttcgttcaa attggatat ggattggcg 
      gcaggagtg gctgtttcg ttactaagt gtttttata 
      caagggtat aaaccctac acttgtctt ttgggggtt 
      gtgccaaac actgacctg tatctggat attaaacgg 
      tacaagaaa gcgcaagaa attcaaggt gtgaagatt 
      tttaggtac tcgggaggg ttgagtttt gcgtcaaga 
      agcgctttt aaggagttg ttgaatagg aaaatcggg 
      tttgatcca gcgagtgtg ctcaggaag agggccagg 
      ttggaggag tcgccctca aggtccacc accgtcacc 
      gaggacttc gatttgctc acccgttgt gtgattttg 
      gacttcgcc tctttgact ttcgtggac ccttctggt 
      gtggactta ctccggcaa ctacagagt gattacgca 
      aaactcgat ataaagctc tacattgcg gcttgttca 
      gggccggtt tatgaaaaa ttcatcatc tgtgatcaa 
      caagaaggc atcgagagc aagtttatg atcttcccg 
      acgattcac gatgccgtt cttttcgct caatcaaat 
      gtgttaagg actaaacaa atttaa
    mRNA sequence
    • >TC015542 RA
      tgcgacatt ctatattac agtcgcttt gcgaccaag 
      taattaaac ggggccccg gcgggccca cttttaatt 
      aaccaaaat attaattaa cctcgtttc ggccagttt 
      tgaaatagc agtgaaaca ataaaattt taaggatcc 
      cccgaaaat aaaaaatcc gtaacaatt gtcagtgat 
      cgtccttcg attgacaca aaaccaact tctaaccta 
      agatccgtt cgaaaaacg attatcctc ccgcaaggt 
      ctgtaatat caattgacc ccgagtgta gatgaaaaa 
      atccgtgta aaatcatca tcataattg cgataaagt 
      tcgtttttg attgtgtga taaggcgtt acgtcattt 
      tgaatgtca ttgtcaggt ttggtggcc aggaccggt 
      ccgagatgt tttcgggcc ctaattatc ggaaaaagc 
      gcgttttca acgaccagg gtcaccttt ggccagatt 
      cgccgcaaa aactaaagt gattattag aataaactt 
      gataataaa ttacgaaaa agtgtgacc ttgggggaa 
      tagcaccga gcgatcagt ttgccaatt cgcgctgat 
      ttaatcaca cgtcaaggt agaagttct aataacttg 
      ataagaatt gtcgagcca gacaacaaa tagagtaaa 
      ttacgccaa ttacggctt aattgtgcc tacttatcg 
      cgtattaat aaaaattcg agtgcacat catttgtat 
      cttttaaag tgagtggca cgccatttg aagataaaa 
      caatcgaaa tatcggtca gtcatcgag tgatcgtcg 
      gaatgtgtg gacctgaac catgaagaa aagcaatac 
      ggacctgaa cggcgtttt ggaaaatgg aacagccgg 
      gctgagtgt gagacggcc ggtttacca acttgaaga 
      tttgaacag agacactga ctatgagac accgcacac 
      aacatttgt gcaggattg tggtcgcag atttcgggc 
      cttccggcc ctttaggtg tctctacta ttcggtgcc 
      ggtgctcaa atggctccc aaaatacag ttgcaagaa 
      aaatatttt tggggatat tatcagtgg gattacggt 
      ggctatcat gcacatacc gcagggcat ggcgtacgg 
      cctcctcgg caacgtgcc ccccgtggt cggcatcta 
      catggcctt cttccccgt acttatcta cttcatttt 
      cggcacatc tcgccacgt ctcgatagg aaccttcgc 
      aatcatttg tctaatgac cggcaaagt ggtcaacca 
      atacagcag catcgaaat cctccaaaa cggcaccgt 
      ggtcaccac cccgtcccc caaccccga aatgcccct 
      ctacaccaa cgtggaagt cgccacaac cgtcacctt 
      cgccgtggc tatgatcca actcgtcat gtactccct 
      gcgccttgg cgtcgtctc gactttact cagcgaaac 
      tctagtgaa cggtttcac gtgtgcttc agccttcca 
      cgtggtctc gtcacaaat caaagatct gttcgggat 
      tccgattaa aaaacgaag gggaaattt cggcttccc 
      tttgacgat ttacgactc ggttttggc cctgtcaag 
      ggccaatcc gtacgcttg tggcatgtc ggcagtatc 
      gtgcgttat ccttatcat caataacga agttttgaa 
      gcccttttt agccaagaa aaccaaaat tccgttccc 
      gatcgaatt gctggctgt ggttttggg tacggcctc 
      gagttattt ctttagttt ggatacgaa gtacgatat 
      ttcggttgt ggggcacat accgactgg gtttccggc 
      ccccacacc tccagcttt tgcccttat accggatat 
      tttagtcga tgcgtttgt aataaccat ggtttcgta 
      cactataac tatgtccat ggcgttgat ttttgcaag 
      gaagctgtt ctatgaggt ggactcgaa ccaggaact 
      gttggcttt gggactgag caacaccat gggctcgtt 
      cttcgcctg catgcccgt cacagcctc cctctcccg 
      ctccatgat ccaggaggc cgtcggagg agtgaccca 
      aatcgcctc catcgtctc ctgcagcat tctcttggt 
      catcctcct ctggatcgg gccactctt cgaaaccct 
      cccccggtg tgtcctagc tagcatcat cgtggtggc 
      cctcaaagg catgctctt ccaatgcca atcgatcgt 
      aaggtattg gaaattgag taaatggga cgctttggt 
      ctggacggt tactttctg tacaacttt gttcgttca 
      aattggata tggattggc ggcaggagt ggctgtttc 
      gttactaag tgtttttat acaagggta taaacccta 
      cacttgtct tttgggggt tgtgccaaa cactgacct 
      gtatctgga tattaaacg gtacaagaa agcgcaaga 
      aattcaagg tgtgaagat ttttaggta ctcgggagg 
      gttgagttt tgcgtcaag aagcgcttt taaggagtt 
      gttgaatag gaaaatcgg gtttgatcc agcgagtgt 
      gctcaggaa gagggccag gttggagga gtcgccctc 
      aaggtccac caccgtcac cgaggactt cgatttgct 
      cacccgttg tgtgatttt ggacttcgc ctctttgac 
      tttcgtgga cccttctgg tgtggactt actccggca 
      actacagag tgattacgc aaaactcga tataaagct 
      ctacattgc ggcttgttc agggccggt ttatgaaaa 
      attcatcat ctgtgatca acaagaagg catcgagag 
      caagtttat gatcttccc gacgattca cgatgccgt 
      tcttttcgc tcaatcaaa tgtgttaag gactaaaca 
      aatttaaat aagtgaaag tattagaga ttttatttt 
      tgttataaa taaatatat ttcgtactt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY