| General information | |||
| TC015542 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 2079705..2097175 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC015542 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05836 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC015542 RA
MKKSNTDLN GVLENGTAG LSVRRPVYQ LEDLNRDTD YETPHTTFV QDCGRRFRA FRPFRCLYY SVPVLKWLP KYSCKKNIF GDIISGITV AIMHIPQGM AYGLLGNVP PVVGIYMAF FPVLIYFIF GTSRHVSIG TFAIICLMT GKVVNQYSS IEILQNGTV VTTPSPNPE MPLYTNVEV ATTVTFAVA MIQLVMYSL RLGVVSTLL SETLVNGFT CASAFHVVS SQIKDLFGI PIKKRRGNF GFPLTIYDS VLALSRANP YACGMSAVS CVILIINNE VLKPFLAKK TKIPFPIEL LAVVLGTAS SYFFSLDTK YDISVVGHI PTGFPAPTP PAFALIPDI LVDAFVITM VSYTITMSM ALIFARKLF YEVDSNQEL LALGLSNTM GSFFACMPV TASLSRSMI QEAVGGVTQ IASIVSCSI LLVILLWIG PLFETLPRC VLASIIVVA LKGMLFQCQ SIVRYWKLS KWDALVWTV TFCTTLFVQ IGYGLAAGV AVSLLSVFI QGYKPYTCL LGVVPNTDL YLDIKRYKK AQEIQGVKI FRYSGGLSF ASRSAFKEL LNRKIGFDP ASVLRKRAR LEESPSRST TVTEDFDLL TRCVILDFA SLTFVDPSG VDLLRQLQS DYAKLDIKL YIAACSGPV YEKFIICDQ QEGIESKFM IFPTIHDAV LFAQSNVLR TKQI
- >TC015542 RA
atgaagaaa agcaatacg gacctgaac ggcgttttg gaaaatgga acagccggg ctgagtgtg agacggccg gtttaccaa cttgaagat ttgaacaga gacactgac tatgagaca ccgcacaca acatttgtg caggattgt ggtcgcaga tttcgggcc ttccggccc tttaggtgt ctctactat tcggtgccg gtgctcaaa tggctccca aaatacagt tgcaagaaa aatattttt ggggatatt atcagtggg attacggtg gctatcatg cacataccg cagggcatg gcgtacggc ctcctcggc aacgtgccc cccgtggtc ggcatctac atggccttc ttccccgta cttatctac ttcattttc ggcacatct cgccacgtc tcgatagga accttcgca atcatttgt ctaatgacc ggcaaagtg gtcaaccaa tacagcagc atcgaaatc ctccaaaac ggcaccgtg gtcaccacc ccgtccccc aaccccgaa atgcccctc tacaccaac gtggaagtc gccacaacc gtcaccttc gccgtggct atgatccaa ctcgtcatg tactccctg cgccttggc gtcgtctcg actttactc agcgaaact ctagtgaac ggtttcacg tgtgcttca gccttccac gtggtctcg tcacaaatc aaagatctg ttcgggatt ccgattaaa aaacgaagg ggaaatttc ggcttccct ttgacgatt tacgactcg gttttggcc ctgtcaagg gccaatccg tacgcttgt ggcatgtcg gcagtatcg tgcgttatc cttatcatc aataacgaa gttttgaag cccttttta gccaagaaa accaaaatt ccgttcccg atcgaattg ctggctgtg gttttgggt acggcctcg agttatttc tttagtttg gatacgaag tacgatatt tcggttgtg gggcacata ccgactggg tttccggcc cccacacct ccagctttt gcccttata ccggatatt ttagtcgat gcgtttgta ataaccatg gtttcgtac actataact atgtccatg gcgttgatt tttgcaagg aagctgttc tatgaggtg gactcgaac caggaactg ttggctttg ggactgagc aacaccatg ggctcgttc ttcgcctgc atgcccgtc acagcctcc ctctcccgc tccatgatc caggaggcc gtcggagga gtgacccaa atcgcctcc atcgtctcc tgcagcatt ctcttggtc atcctcctc tggatcggg ccactcttc gaaaccctc ccccggtgt gtcctagct agcatcatc gtggtggcc ctcaaaggc atgctcttc caatgccaa tcgatcgta aggtattgg aaattgagt aaatgggac gctttggtc tggacggtt actttctgt acaactttg ttcgttcaa attggatat ggattggcg gcaggagtg gctgtttcg ttactaagt gtttttata caagggtat aaaccctac acttgtctt ttgggggtt gtgccaaac actgacctg tatctggat attaaacgg tacaagaaa gcgcaagaa attcaaggt gtgaagatt tttaggtac tcgggaggg ttgagtttt gcgtcaaga agcgctttt aaggagttg ttgaatagg aaaatcggg tttgatcca gcgagtgtg ctcaggaag agggccagg ttggaggag tcgccctca aggtccacc accgtcacc gaggacttc gatttgctc acccgttgt gtgattttg gacttcgcc tctttgact ttcgtggac ccttctggt gtggactta ctccggcaa ctacagagt gattacgca aaactcgat ataaagctc tacattgcg gcttgttca gggccggtt tatgaaaaa ttcatcatc tgtgatcaa caagaaggc atcgagagc aagtttatg atcttcccg acgattcac gatgccgtt cttttcgct caatcaaat gtgttaagg actaaacaa atttaa
- >TC015542 RA
tgcgacatt ctatattac agtcgcttt gcgaccaag taattaaac ggggccccg gcgggccca cttttaatt aaccaaaat attaattaa cctcgtttc ggccagttt tgaaatagc agtgaaaca ataaaattt taaggatcc cccgaaaat aaaaaatcc gtaacaatt gtcagtgat cgtccttcg attgacaca aaaccaact tctaaccta agatccgtt cgaaaaacg attatcctc ccgcaaggt ctgtaatat caattgacc ccgagtgta gatgaaaaa atccgtgta aaatcatca tcataattg cgataaagt tcgtttttg attgtgtga taaggcgtt acgtcattt tgaatgtca ttgtcaggt ttggtggcc aggaccggt ccgagatgt tttcgggcc ctaattatc ggaaaaagc gcgttttca acgaccagg gtcaccttt ggccagatt cgccgcaaa aactaaagt gattattag aataaactt gataataaa ttacgaaaa agtgtgacc ttgggggaa tagcaccga gcgatcagt ttgccaatt cgcgctgat ttaatcaca cgtcaaggt agaagttct aataacttg ataagaatt gtcgagcca gacaacaaa tagagtaaa ttacgccaa ttacggctt aattgtgcc tacttatcg cgtattaat aaaaattcg agtgcacat catttgtat cttttaaag tgagtggca cgccatttg aagataaaa caatcgaaa tatcggtca gtcatcgag tgatcgtcg gaatgtgtg gacctgaac catgaagaa aagcaatac ggacctgaa cggcgtttt ggaaaatgg aacagccgg gctgagtgt gagacggcc ggtttacca acttgaaga tttgaacag agacactga ctatgagac accgcacac aacatttgt gcaggattg tggtcgcag atttcgggc cttccggcc ctttaggtg tctctacta ttcggtgcc ggtgctcaa atggctccc aaaatacag ttgcaagaa aaatatttt tggggatat tatcagtgg gattacggt ggctatcat gcacatacc gcagggcat ggcgtacgg cctcctcgg caacgtgcc ccccgtggt cggcatcta catggcctt cttccccgt acttatcta cttcatttt cggcacatc tcgccacgt ctcgatagg aaccttcgc aatcatttg tctaatgac cggcaaagt ggtcaacca atacagcag catcgaaat cctccaaaa cggcaccgt ggtcaccac cccgtcccc caaccccga aatgcccct ctacaccaa cgtggaagt cgccacaac cgtcacctt cgccgtggc tatgatcca actcgtcat gtactccct gcgccttgg cgtcgtctc gactttact cagcgaaac tctagtgaa cggtttcac gtgtgcttc agccttcca cgtggtctc gtcacaaat caaagatct gttcgggat tccgattaa aaaacgaag gggaaattt cggcttccc tttgacgat ttacgactc ggttttggc cctgtcaag ggccaatcc gtacgcttg tggcatgtc ggcagtatc gtgcgttat ccttatcat caataacga agttttgaa gcccttttt agccaagaa aaccaaaat tccgttccc gatcgaatt gctggctgt ggttttggg tacggcctc gagttattt ctttagttt ggatacgaa gtacgatat ttcggttgt ggggcacat accgactgg gtttccggc ccccacacc tccagcttt tgcccttat accggatat tttagtcga tgcgtttgt aataaccat ggtttcgta cactataac tatgtccat ggcgttgat ttttgcaag gaagctgtt ctatgaggt ggactcgaa ccaggaact gttggcttt gggactgag caacaccat gggctcgtt cttcgcctg catgcccgt cacagcctc cctctcccg ctccatgat ccaggaggc cgtcggagg agtgaccca aatcgcctc catcgtctc ctgcagcat tctcttggt catcctcct ctggatcgg gccactctt cgaaaccct cccccggtg tgtcctagc tagcatcat cgtggtggc cctcaaagg catgctctt ccaatgcca atcgatcgt aaggtattg gaaattgag taaatggga cgctttggt ctggacggt tactttctg tacaacttt gttcgttca aattggata tggattggc ggcaggagt ggctgtttc gttactaag tgtttttat acaagggta taaacccta cacttgtct tttgggggt tgtgccaaa cactgacct gtatctgga tattaaacg gtacaagaa agcgcaaga aattcaagg tgtgaagat ttttaggta ctcgggagg gttgagttt tgcgtcaag aagcgcttt taaggagtt gttgaatag gaaaatcgg gtttgatcc agcgagtgt gctcaggaa gagggccag gttggagga gtcgccctc aaggtccac caccgtcac cgaggactt cgatttgct cacccgttg tgtgatttt ggacttcgc ctctttgac tttcgtgga cccttctgg tgtggactt actccggca actacagag tgattacgc aaaactcga tataaagct ctacattgc ggcttgttc agggccggt ttatgaaaa attcatcat ctgtgatca acaagaagg catcgagag caagtttat gatcttccc gacgattca cgatgccgt tcttttcgc tcaatcaaa tgtgttaag gactaaaca aatttaaat aagtgaaag tattagaga ttttatttt tgttataaa taaatatat ttcgtactt
