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Gene information
General information
TC015542 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2079705..2097175
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015542
    iBeetle screen iB_05836

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015542 RA
      MKKSNTDLN GVLENGTAG LSVRRPVYQ LEDLNRDTD 
YETPHTTFV QDCGRRFRA FRPFRCLYY SVPVLKWLP 
KYSCKKNIF GDIISGITV AIMHIPQGM AYGLLGNVP 
PVVGIYMAF FPVLIYFIF GTSRHVSIG TFAIICLMT 
GKVVNQYSS IEILQNGTV VTTPSPNPE MPLYTNVEV 
ATTVTFAVA MIQLVMYSL RLGVVSTLL SETLVNGFT 
CASAFHVVS SQIKDLFGI PIKKRRGNF GFPLTIYDS 
VLALSRANP YACGMSAVS CVILIINNE VLKPFLAKK 
TKIPFPIEL LAVVLGTAS SYFFSLDTK YDISVVGHI 
PTGFPAPTP PAFALIPDI LVDAFVITM VSYTITMSM 
ALIFARKLF YEVDSNQEL LALGLSNTM GSFFACMPV 
TASLSRSMI QEAVGGVTQ IASIVSCSI LLVILLWIG 
PLFETLPRC VLASIIVVA LKGMLFQCQ SIVRYWKLS 
KWDALVWTV TFCTTLFVQ IGYGLAAGV AVSLLSVFI 
QGYKPYTCL LGVVPNTDL YLDIKRYKK AQEIQGVKI 
FRYSGGLSF ASRSAFKEL LNRKIGFDP ASVLRKRAR 
LEESPSRST TVTEDFDLL TRCVILDFA SLTFVDPSG 
VDLLRQLQS DYAKLDIKL YIAACSGPV YEKFIICDQ 
QEGIESKFM IFPTIHDAV LFAQSNVLR TKQI
    CDS sequence
    • >TC015542 RA
      atgaagaaa agcaatacg gacctgaac ggcgttttg 
gaaaatgga acagccggg ctgagtgtg agacggccg 
gtttaccaa cttgaagat ttgaacaga gacactgac 
tatgagaca ccgcacaca acatttgtg caggattgt 
ggtcgcaga tttcgggcc ttccggccc tttaggtgt 
ctctactat tcggtgccg gtgctcaaa tggctccca 
aaatacagt tgcaagaaa aatattttt ggggatatt 
atcagtggg attacggtg gctatcatg cacataccg 
cagggcatg gcgtacggc ctcctcggc aacgtgccc 
cccgtggtc ggcatctac atggccttc ttccccgta 
cttatctac ttcattttc ggcacatct cgccacgtc 
tcgatagga accttcgca atcatttgt ctaatgacc 
ggcaaagtg gtcaaccaa tacagcagc atcgaaatc 
ctccaaaac ggcaccgtg gtcaccacc ccgtccccc 
aaccccgaa atgcccctc tacaccaac gtggaagtc 
gccacaacc gtcaccttc gccgtggct atgatccaa 
ctcgtcatg tactccctg cgccttggc gtcgtctcg 
actttactc agcgaaact ctagtgaac ggtttcacg 
tgtgcttca gccttccac gtggtctcg tcacaaatc 
aaagatctg ttcgggatt ccgattaaa aaacgaagg 
ggaaatttc ggcttccct ttgacgatt tacgactcg 
gttttggcc ctgtcaagg gccaatccg tacgcttgt 
ggcatgtcg gcagtatcg tgcgttatc cttatcatc 
aataacgaa gttttgaag cccttttta gccaagaaa 
accaaaatt ccgttcccg atcgaattg ctggctgtg 
gttttgggt acggcctcg agttatttc tttagtttg 
gatacgaag tacgatatt tcggttgtg gggcacata 
ccgactggg tttccggcc cccacacct ccagctttt 
gcccttata ccggatatt ttagtcgat gcgtttgta 
ataaccatg gtttcgtac actataact atgtccatg 
gcgttgatt tttgcaagg aagctgttc tatgaggtg 
gactcgaac caggaactg ttggctttg ggactgagc 
aacaccatg ggctcgttc ttcgcctgc atgcccgtc 
acagcctcc ctctcccgc tccatgatc caggaggcc 
gtcggagga gtgacccaa atcgcctcc atcgtctcc 
tgcagcatt ctcttggtc atcctcctc tggatcggg 
ccactcttc gaaaccctc ccccggtgt gtcctagct 
agcatcatc gtggtggcc ctcaaaggc atgctcttc 
caatgccaa tcgatcgta aggtattgg aaattgagt 
aaatgggac gctttggtc tggacggtt actttctgt 
acaactttg ttcgttcaa attggatat ggattggcg 
gcaggagtg gctgtttcg ttactaagt gtttttata 
caagggtat aaaccctac acttgtctt ttgggggtt 
gtgccaaac actgacctg tatctggat attaaacgg 
tacaagaaa gcgcaagaa attcaaggt gtgaagatt 
tttaggtac tcgggaggg ttgagtttt gcgtcaaga 
agcgctttt aaggagttg ttgaatagg aaaatcggg 
tttgatcca gcgagtgtg ctcaggaag agggccagg 
ttggaggag tcgccctca aggtccacc accgtcacc 
gaggacttc gatttgctc acccgttgt gtgattttg 
gacttcgcc tctttgact ttcgtggac ccttctggt 
gtggactta ctccggcaa ctacagagt gattacgca 
aaactcgat ataaagctc tacattgcg gcttgttca 
gggccggtt tatgaaaaa ttcatcatc tgtgatcaa 
caagaaggc atcgagagc aagtttatg atcttcccg 
acgattcac gatgccgtt cttttcgct caatcaaat 
gtgttaagg actaaacaa atttaa
    mRNA sequence
    • >TC015542 RA
      tgcgacatt ctatattac agtcgcttt gcgaccaag 
taattaaac ggggccccg gcgggccca cttttaatt 
aaccaaaat attaattaa cctcgtttc ggccagttt 
tgaaatagc agtgaaaca ataaaattt taaggatcc 
cccgaaaat aaaaaatcc gtaacaatt gtcagtgat 
cgtccttcg attgacaca aaaccaact tctaaccta 
agatccgtt cgaaaaacg attatcctc ccgcaaggt 
ctgtaatat caattgacc ccgagtgta gatgaaaaa 
atccgtgta aaatcatca tcataattg cgataaagt 
tcgtttttg attgtgtga taaggcgtt acgtcattt 
tgaatgtca ttgtcaggt ttggtggcc aggaccggt 
ccgagatgt tttcgggcc ctaattatc ggaaaaagc 
gcgttttca acgaccagg gtcaccttt ggccagatt 
cgccgcaaa aactaaagt gattattag aataaactt 
gataataaa ttacgaaaa agtgtgacc ttgggggaa 
tagcaccga gcgatcagt ttgccaatt cgcgctgat 
ttaatcaca cgtcaaggt agaagttct aataacttg 
ataagaatt gtcgagcca gacaacaaa tagagtaaa 
ttacgccaa ttacggctt aattgtgcc tacttatcg 
cgtattaat aaaaattcg agtgcacat catttgtat 
cttttaaag tgagtggca cgccatttg aagataaaa 
caatcgaaa tatcggtca gtcatcgag tgatcgtcg 
gaatgtgtg gacctgaac catgaagaa aagcaatac 
ggacctgaa cggcgtttt ggaaaatgg aacagccgg 
gctgagtgt gagacggcc ggtttacca acttgaaga 
tttgaacag agacactga ctatgagac accgcacac 
aacatttgt gcaggattg tggtcgcag atttcgggc 
cttccggcc ctttaggtg tctctacta ttcggtgcc 
ggtgctcaa atggctccc aaaatacag ttgcaagaa 
aaatatttt tggggatat tatcagtgg gattacggt 
ggctatcat gcacatacc gcagggcat ggcgtacgg 
cctcctcgg caacgtgcc ccccgtggt cggcatcta 
catggcctt cttccccgt acttatcta cttcatttt 
cggcacatc tcgccacgt ctcgatagg aaccttcgc 
aatcatttg tctaatgac cggcaaagt ggtcaacca 
atacagcag catcgaaat cctccaaaa cggcaccgt 
ggtcaccac cccgtcccc caaccccga aatgcccct 
ctacaccaa cgtggaagt cgccacaac cgtcacctt 
cgccgtggc tatgatcca actcgtcat gtactccct 
gcgccttgg cgtcgtctc gactttact cagcgaaac 
tctagtgaa cggtttcac gtgtgcttc agccttcca 
cgtggtctc gtcacaaat caaagatct gttcgggat 
tccgattaa aaaacgaag gggaaattt cggcttccc 
tttgacgat ttacgactc ggttttggc cctgtcaag 
ggccaatcc gtacgcttg tggcatgtc ggcagtatc 
gtgcgttat ccttatcat caataacga agttttgaa 
gcccttttt agccaagaa aaccaaaat tccgttccc 
gatcgaatt gctggctgt ggttttggg tacggcctc 
gagttattt ctttagttt ggatacgaa gtacgatat 
ttcggttgt ggggcacat accgactgg gtttccggc 
ccccacacc tccagcttt tgcccttat accggatat 
tttagtcga tgcgtttgt aataaccat ggtttcgta 
cactataac tatgtccat ggcgttgat ttttgcaag 
gaagctgtt ctatgaggt ggactcgaa ccaggaact 
gttggcttt gggactgag caacaccat gggctcgtt 
cttcgcctg catgcccgt cacagcctc cctctcccg 
ctccatgat ccaggaggc cgtcggagg agtgaccca 
aatcgcctc catcgtctc ctgcagcat tctcttggt 
catcctcct ctggatcgg gccactctt cgaaaccct 
cccccggtg tgtcctagc tagcatcat cgtggtggc 
cctcaaagg catgctctt ccaatgcca atcgatcgt 
aaggtattg gaaattgag taaatggga cgctttggt 
ctggacggt tactttctg tacaacttt gttcgttca 
aattggata tggattggc ggcaggagt ggctgtttc 
gttactaag tgtttttat acaagggta taaacccta 
cacttgtct tttgggggt tgtgccaaa cactgacct 
gtatctgga tattaaacg gtacaagaa agcgcaaga 
aattcaagg tgtgaagat ttttaggta ctcgggagg 
gttgagttt tgcgtcaag aagcgcttt taaggagtt 
gttgaatag gaaaatcgg gtttgatcc agcgagtgt 
gctcaggaa gagggccag gttggagga gtcgccctc 
aaggtccac caccgtcac cgaggactt cgatttgct 
cacccgttg tgtgatttt ggacttcgc ctctttgac 
tttcgtgga cccttctgg tgtggactt actccggca 
actacagag tgattacgc aaaactcga tataaagct 
ctacattgc ggcttgttc agggccggt ttatgaaaa 
attcatcat ctgtgatca acaagaagg catcgagag 
caagtttat gatcttccc gacgattca cgatgccgt 
tcttttcgc tcaatcaaa tgtgttaag gactaaaca 
aatttaaat aagtgaaag tattagaga ttttatttt 
tgttataaa taaatatat ttcgtactt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY