| General information | |||
| TC030900
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 1903396..1977626 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC030900 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05834 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
labrum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
mandible not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
labium not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
labrum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
mandible not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
labium not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC030900 RA
MAEVNNRPV FKMNNDFWN QARGPPQSM AQNLSNSNQ NSPVPGSTN SIPAHSPSQ QSNSSQNHV IQQQPQNQQ QPQQQQQQQ NSVQQQNNG QQQQQQQQQ PNQQQQQQQ QQQQLGSPN SQVAQAHAH ALAQVQAQQ QLVHMSQQH QLNAGQNSP DKMAEKLVN DLQIFMHKS AMQMHAREL TGRSEVKPH QCQQCLKSF SSNHQLVQH IRVHTGEKP YKCSYCDRR FKQLSHVQQ HTRLHTGER PYKCHLPDC GRAFIQLSN LQQHLRNHD AQVERAKNR PFHCTICGK GFATESSLR THTSKVSHC IGRLQQHAA LIGGPNATS CPLCHKMFL GGEALMEHM KHTHKDPNA SGVATKRRT ANHPCPVCG KHYVNEGSL RKHLACHPE TSQLSSSLR MWPCSVCQA VFTHESGLL THMEHMRMD TKHQFAAQY VLSRAAAER RERETLLAA VSASASGSG LLGLTGMAG AASGSPMCA SPSAHSDSS SGNGRLSSA GSEAGGLNN NNNNCTTKL NEILRSNNG LHQQYNVEE QFHNANRMA VIANMVGQG GVPPGLGSD SSAAANIAA ANIANLAMR LGVTQANQV NNSSNPSTP PINNDTLSA SMNAMNAMR ASMDSIRNM DAMRSSVMD MSSPQQSQQ QQDALRMQQ HAEALLRSQ AEAALRLAM SQALPQLNQ HQQDNGSPL RHNGNYQPH QGQSSQQLS PDLTEALRL QEQRLEQAL RLHGSDPRT LGFSLSSQQ QNQQP
- >TC030900 RB
MAEVNNRPV FKMNNDFWN QARGPPQSM AQNLSNSNQ NSPVPGSTN SIPAHSPSQ QSNSSQNHV IQQQPQNQQ QPQQQQQQQ NSVQQQNNG QQQQQQQQQ PNQQQQQQQ QQQQLGSPN SQVAQAHAH ALAQVQAQQ QLVHMSQQH QLNAGQNSP DKMAEKLVN DLQIFMHKS AMQMHAREL TGRSEVKPH QCQQCLKSF SSNHQLVQH IRVHTGEKP YKCSYCDRR FKQLSHVQQ HTRLHTGER PYKCHLPDC GRAFIQLSN LQQHLRNHD AQVERAKNR PFHCTICGK GFATESSLR THTSKVSHQ HAALIGGPN ATSCPLCHK MFLGGEALM EHMKHTHKD PNASGVATK RRTANHPCP VCGKHYVNE GSLRKHLAC HPETSQLSS SLRMWPCSV CQAVFTHES GLLTHMEHM RMDTKHQFA AQYVLSRAA AERRERETL LAAVSASAS GSGLLGLTG MAGAASGSP MCASPSAHS DSSSGNGRL SSAGSEAGG LNNNNNNCT TKLNEILRS NNGLHQQYN VEEQFHNAN RMAVIANMV GQGGVPPGL GSDSSAAAN IAAANIANL AMRLGVTQA NQVNNSSNP STPPINNDT LSASMNAMN AMRASMDSI RNMDAMRSS VMDMSSPQQ SQQQQDALR MQQHAEALL RSQAEAALR LAMSQALPQ LNQHQQDNG SPLRHNGNY QPHQGQSSQ QLSPDLTEA LRLQEQRLE QALRLHGSD PRTLGFSLS SQQQNQQP
- >TC030900 RC
MAEVNNRPV FKMNNDFWN QARGPPQSM AQNLSNSNQ NSPVPGSTN SIPAHSPSQ QSNSSQNHV IQQQPQNQQ QPQQQQQQQ NSVQQQNNG QQQQQQQQQ PNQQQQQQQ QQQQLGSPN SQVAQAHAH ALAQVQAQQ QLVHMSQQH QLNAGQNSP DKMAEKLVN DLQLLSQLT LPDFNQLQR NSTSSAISE RTLEECWTT LQRIFMHKS AMQMHAREL TGRSEVKPH QCQQCLKSF SSNHQLVQH IRVHTGEKP YKCSYCDRR FKQLSHVQQ HTRLHTGER PYKCHLPDC GRAFIQLSN LQQHLRNHD AQVERAKNR PFHCTICGK GFATESSLR THTSKVSHC IGRLQQHAA LIGGPNATS CPLCHKMFL GGEALMEHM KHTHKDPNA SGVATKRRT ANHPCPVCG KHYVNEGSL RKHLACHPE TSQLSSSLR MWPCSVCQA VFTHESGLL THMEHMRMD TKHQFAAQY VLSRAAAER RERETLLAA VSASASGSG LLGLTGMAG AASGSPMCA SPSAHSDSS SGNGRLSSA GSEAGGLNN NNNNCTTKL NEILRSNNG LHQQYNVEE QFHNANRMA VIANMVGQG GVPPGLGSD SSAAANIAA ANIANLAMR LGVTQANQV NNSSNPSTP PINNDTLSA SMNAMNAMR ASMDSIRNM DAMRSSVMD MSSPQQSQQ QQDALRMQQ HAEALLRSQ AEAALRLAM SQALPQLNQ HQQDNGSPL RHNGNYQPH QGQSSQQLS PDLTEALRL QEQRLEQAL RLHGSDPRT LGFSLSSQQ QNQQP
- >TC030900 RA
atggccgag gtcaacaac aggcccgtc ttcaagatg aacaacgac ttctggaac caggcccgc ggcccgccc cagagcatg gcccagaac ttgtccaac tcgaaccag aactcgccg gtgccgggc tccaccaac tccatcccg gcccactcg ccatcgcag cagagcaac agcagccaa aaccacgtc atacaacaa caaccacaa aaccaacaa caaccccaa caacaacaa caacaacaa aactcggta cagcaacaa aacaacggc caacaacaa cagcagcaa caacagcaa ccgaatcaa caacagcaa cagcaacag caacagcag caactgggc agtcccaac tcgcaggtg gcgcaggcc catgcccat gccctggcc caggtccag gcccaacag cagttggtg cacatgtca caacagcac caactcaac gctgggcaa aattcgccg gacaaaatg gccgaaaag ttggtcaat gatttgcag attttcatg cacaaatct gcgatgcaa atgcatgcc agggagctg acgggacgc tcagaagtc aaaccacac cagtgccag caatgtcta aagtctttc agcagcaat caccagtta gtccaacat atcagggtg cacacgggg gaaaaaccc tacaaatgt tcctattgt gacaggagg ttcaagcag ttgagtcac gtgcaacag cacacgagg ctacatacg ggtgagagg ccctacaag tgccacttg cctgactgc ggaagggcc tttatccaa ctgtccaac cttcagcag catctcaga aaccacgac gctcaggtc gaaagggcc aaaaacagg cccttccac tgcaccatc tgtggaaaa gggttcgcc accgagtcc agtctaagg acccatacg tcgaaagta agccattgt attggtcgt ttgcagcaa catgcagct ctgattggt ggacccaac gccacttct tgtccgctt tgccataag atgttctta ggaggagag gcgctcatg gagcacatg aagcacaca cacaaggac cccaatgcc tcgggggtt gccacgaag agaaggaca gcgaatcat ccttgtccc gtttgtgga aagcattat gtgaatgaa ggatccctc aggaaacat ttggcgtgc catcccgaa acgtcacaa ttgtcaagt tcgttgaga atgtggccg tgttctgtt tgtcaagca gtttttacg cacgaatca ggtcttctc acgcacatg gagcacatg aggatggac accaagcac cagttcgcc gcccaatat gtgctgtca cgcgccgcc gccgaaagg agagaacga gagactttg ctggcggcc gtttccgct tccgcctcc gggagcggc cttctaggc ctcacagga atggccggg gcagcgagc ggtagccca atgtgtgca tcccccagc gctcacagt gacagttcc tcaggcaat ggccgcctt tcctctgcc ggttcagaa gccggcggt cttaacaac aacaacaac aattgtaca acaaaactg aacgagatt ttacggagc aacaacggt ctccaccaa cagtacaac gtcgaagaa cagtttcat aatgctaac agaatggcc gtcattgcc aacatggtg ggccaaggg ggggttccc ccaggactc ggctccgac tcgtcggcc gctgccaac atcgccgcc gccaatatt gccaacctt gccatgcgc ctcggcgtc acacaggcc aatcaggtg aataacagc agcaacccc tcaacgccc cccatcaac aatgacact ctttccgcg tccatgaac gcgatgaac gccatgcgg gcgtccatg gacagcata agaaacatg gacgcgatg cggtcgtcg gtcatggac atgtcgtcg cctcagcag tcccagcag cagcaggac gcgctcagg atgcagcag cacgccgag gcgctgctg cggtcgcag gccgaggcg gcgctcaga ctggccatg agccaggcg ctgccccag ctgaaccag caccagcag gacaacggc agccccctg cggcacaac ggcaactat cagccgcac caagggcag tcgtcgcag cagctgagt ccggacctg accgaggcc ctgcggctg caggagcag cggctggag caggccctg cggctgcac gggagcgac ccccggact ctgggcttc tccctcagc agccagcag cagaaccaa cagccatga
- >TC030900 RB
atggccgag gtcaacaac aggcccgtc ttcaagatg aacaacgac ttctggaac caggcccgc ggcccgccc cagagcatg gcccagaac ttgtccaac tcgaaccag aactcgccg gtgccgggc tccaccaac tccatcccg gcccactcg ccatcgcag cagagcaac agcagccaa aaccacgtc atacaacaa caaccacaa aaccaacaa caaccccaa caacaacaa caacaacaa aactcggta cagcaacaa aacaacggc caacaacaa cagcagcaa caacagcaa ccgaatcaa caacagcaa cagcaacag caacagcag caactgggc agtcccaac tcgcaggtg gcgcaggcc catgcccat gccctggcc caggtccag gcccaacag cagttggtg cacatgtca caacagcac caactcaac gctgggcaa aattcgccg gacaaaatg gccgaaaag ttggtcaat gatttgcag attttcatg cacaaatct gcgatgcaa atgcatgcc agggagctg acgggacgc tcagaagtc aaaccacac cagtgccag caatgtcta aagtctttc agcagcaat caccagtta gtccaacat atcagggtg cacacgggg gaaaaaccc tacaaatgt tcctattgt gacaggagg ttcaagcag ttgagtcac gtgcaacag cacacgagg ctacatacg ggtgagagg ccctacaag tgccacttg cctgactgc ggaagggcc tttatccaa ctgtccaac cttcagcag catctcaga aaccacgac gctcaggtc gaaagggcc aaaaacagg cccttccac tgcaccatc tgtggaaaa gggttcgcc accgagtcc agtctaagg acccatacg tcgaaagta agccatcaa catgcagct ctgattggt ggacccaac gccacttct tgtccgctt tgccataag atgttctta ggaggagag gcgctcatg gagcacatg aagcacaca cacaaggac cccaatgcc tcgggggtt gccacgaag agaaggaca gcgaatcat ccttgtccc gtttgtgga aagcattat gtgaatgaa ggatccctc aggaaacat ttggcgtgc catcccgaa acgtcacaa ttgtcaagt tcgttgaga atgtggccg tgttctgtt tgtcaagca gtttttacg cacgaatca ggtcttctc acgcacatg gagcacatg aggatggac accaagcac cagttcgcc gcccaatat gtgctgtca cgcgccgcc gccgaaagg agagaacga gagactttg ctggcggcc gtttccgct tccgcctcc gggagcggc cttctaggc ctcacagga atggccggg gcagcgagc ggtagccca atgtgtgca tcccccagc gctcacagt gacagttcc tcaggcaat ggccgcctt tcctctgcc ggttcagaa gccggcggt cttaacaac aacaacaac aattgtaca acaaaactg aacgagatt ttacggagc aacaacggt ctccaccaa cagtacaac gtcgaagaa cagtttcat aatgctaac agaatggcc gtcattgcc aacatggtg ggccaaggg ggggttccc ccaggactc ggctccgac tcgtcggcc gctgccaac atcgccgcc gccaatatt gccaacctt gccatgcgc ctcggcgtc acacaggcc aatcaggtg aataacagc agcaacccc tcaacgccc cccatcaac aatgacact ctttccgcg tccatgaac gcgatgaac gccatgcgg gcgtccatg gacagcata agaaacatg gacgcgatg cggtcgtcg gtcatggac atgtcgtcg cctcagcag tcccagcag cagcaggac gcgctcagg atgcagcag cacgccgag gcgctgctg cggtcgcag gccgaggcg gcgctcaga ctggccatg agccaggcg ctgccccag ctgaaccag caccagcag gacaacggc agccccctg cggcacaac ggcaactat cagccgcac caagggcag tcgtcgcag cagctgagt ccggacctg accgaggcc ctgcggctg caggagcag cggctggag caggccctg cggctgcac gggagcgac ccccggact ctgggcttc tccctcagc agccagcag cagaaccaa cagccatga
- >TC030900 RC
atggccgag gtcaacaac aggcccgtc ttcaagatg aacaacgac ttctggaac caggcccgc ggcccgccc cagagcatg gcccagaac ttgtccaac tcgaaccag aactcgccg gtgccgggc tccaccaac tccatcccg gcccactcg ccatcgcag cagagcaac agcagccaa aaccacgtc atacaacaa caaccacaa aaccaacaa caaccccaa caacaacaa caacaacaa aactcggta cagcaacaa aacaacggc caacaacaa cagcagcaa caacagcaa ccgaatcaa caacagcaa cagcaacag caacagcag caactgggc agtcccaac tcgcaggtg gcgcaggcc catgcccat gccctggcc caggtccag gcccaacag cagttggtg cacatgtca caacagcac caactcaac gctgggcaa aattcgccg gacaaaatg gccgaaaag ttggtcaat gatttgcag ttgctttcc caactaacg ctgccggac tttaaccaa ctgcagagg aattcgact tcttcggcc atctccgaa aggacgctt gaagaatgt tggaccaca ctgcagcga attttcatg cacaaatct gcgatgcaa atgcatgcc agggagctg acgggacgc tcagaagtc aaaccacac cagtgccag caatgtcta aagtctttc agcagcaat caccagtta gtccaacat atcagggtg cacacgggg gaaaaaccc tacaaatgt tcctattgt gacaggagg ttcaagcag ttgagtcac gtgcaacag cacacgagg ctacatacg ggtgagagg ccctacaag tgccacttg cctgactgc ggaagggcc tttatccaa ctgtccaac cttcagcag catctcaga aaccacgac gctcaggtc gaaagggcc aaaaacagg cccttccac tgcaccatc tgtggaaaa gggttcgcc accgagtcc agtctaagg acccatacg tcgaaagta agccattgt attggtcgt ttgcagcaa catgcagct ctgattggt ggacccaac gccacttct tgtccgctt tgccataag atgttctta ggaggagag gcgctcatg gagcacatg aagcacaca cacaaggac cccaatgcc tcgggggtt gccacgaag agaaggaca gcgaatcat ccttgtccc gtttgtgga aagcattat gtgaatgaa ggatccctc aggaaacat ttggcgtgc catcccgaa acgtcacaa ttgtcaagt tcgttgaga atgtggccg tgttctgtt tgtcaagca gtttttacg cacgaatca ggtcttctc acgcacatg gagcacatg aggatggac accaagcac cagttcgcc gcccaatat gtgctgtca cgcgccgcc gccgaaagg agagaacga gagactttg ctggcggcc gtttccgct tccgcctcc gggagcggc cttctaggc ctcacagga atggccggg gcagcgagc ggtagccca atgtgtgca tcccccagc gctcacagt gacagttcc tcaggcaat ggccgcctt tcctctgcc ggttcagaa gccggcggt cttaacaac aacaacaac aattgtaca acaaaactg aacgagatt ttacggagc aacaacggt ctccaccaa cagtacaac gtcgaagaa cagtttcat aatgctaac agaatggcc gtcattgcc aacatggtg ggccaaggg ggggttccc ccaggactc ggctccgac tcgtcggcc gctgccaac atcgccgcc gccaatatt gccaacctt gccatgcgc ctcggcgtc acacaggcc aatcaggtg aataacagc agcaacccc tcaacgccc cccatcaac aatgacact ctttccgcg tccatgaac gcgatgaac gccatgcgg gcgtccatg gacagcata agaaacatg gacgcgatg cggtcgtcg gtcatggac atgtcgtcg cctcagcag tcccagcag cagcaggac gcgctcagg atgcagcag cacgccgag gcgctgctg cggtcgcag gccgaggcg gcgctcaga ctggccatg agccaggcg ctgccccag ctgaaccag caccagcag gacaacggc agccccctg cggcacaac ggcaactat cagccgcac caagggcag tcgtcgcag cagctgagt ccggacctg accgaggcc ctgcggctg caggagcag cggctggag caggccctg cggctgcac gggagcgac ccccggact ctgggcttc tccctcagc agccagcag cagaaccaa cagccatga
- >TC030900 RA
ccggagtgc acaggtgat ccgattttt gtgaggttc aagtggcat aatgtggct caaagtgac cgatgattg gaattgagt aaagcgatc ggtaccaat ccggactcg tatttcgtc atcgtctcc gaacccctt taccacgac gccaataac ccagccatc gcgctcgcc acaccgtca accggccac cacatccac ccctagtcc catcgtcgg tactagagc aaaattagt gccgttcga gcgttggct gcgccttct tcttccgtc aaataaagt cgtcgtccc tcacgggcc gtattctcg ccttttccc gccccgccg tcaacattt tcaacacgg aggaccaaa atctaatca acaatctca aggtcgaac atgaaaacc tcgtagcga agtggctgc agaactcat tcaaggccc aggagaacg ccgcactgg acctccggt cgacgccgc tgtcgctgc agataccga tggccgagg tcaacaaca ggcccgtct tcaagatga acaacgact tctggaacc aggcccgcg gcccgcccc agagcatgg cccagaact tgtccaact cgaaccaga actcgccgg tgccgggct ccaccaact ccatcccgg cccactcgc catcgcagc agagcaaca gcagccaaa accacgtca tacaacaac aaccacaaa accaacaac aaccccaac aacaacaac aacaacaaa actcggtac agcaacaaa acaacggcc aacaacaac agcagcaac aacagcaac cgaatcaac aacagcaac agcaacagc aacagcagc aactgggca gtcccaact cgcaggtgg cgcaggccc atgcccatg ccctggccc aggtccagg cccaacagc agttggtgc acatgtcac aacagcacc aactcaacg ctgggcaaa attcgccgg acaaaatgg ccgaaaagt tggtcaatg atttgcaga ttttcatgc acaaatctg cgatgcaaa tgcatgcca gggagctga cgggacgct cagaagtca aaccacacc agtgccagc aatgtctaa agtctttca gcagcaatc accagttag tccaacata tcagggtgc acacggggg aaaaaccct acaaatgtt cctattgtg acaggaggt tcaagcagt tgagtcacg tgcaacagc acacgaggc tacatacgg gtgagaggc cctacaagt gccacttgc ctgactgcg gaagggcct ttatccaac tgtccaacc ttcagcagc atctcagaa accacgacg ctcaggtcg aaagggcca aaaacaggc ccttccact gcaccatct gtggaaaag ggttcgcca ccgagtcca gtctaagga cccatacgt cgaaagtaa gccattgta ttggtcgtt tgcagcaac atgcagctc tgattggtg gacccaacg ccacttctt gtccgcttt gccataaga tgttcttag gaggagagg cgctcatgg agcacatga agcacacac acaaggacc ccaatgcct cgggggttg ccacgaaga gaaggacag cgaatcatc cttgtcccg tttgtggaa agcattatg tgaatgaag gatccctca ggaaacatt tggcgtgcc atcccgaaa cgtcacaat tgtcaagtt cgttgagaa tgtggccgt gttctgttt gtcaagcag tttttacgc acgaatcag gtcttctca cgcacatgg agcacatga ggatggaca ccaagcacc agttcgccg cccaatatg tgctgtcac gcgccgccg ccgaaagga gagaacgag agactttgc tggcggccg tttccgctt ccgcctccg ggagcggcc ttctaggcc tcacaggaa tggccgggg cagcgagcg gtagcccaa tgtgtgcat cccccagcg ctcacagtg acagttcct caggcaatg gccgccttt cctctgccg gttcagaag ccggcggtc ttaacaaca acaacaaca attgtacaa caaaactga acgagattt tacggagca acaacggtc tccaccaac agtacaacg tcgaagaac agtttcata atgctaaca gaatggccg tcattgcca acatggtgg gccaagggg gggttcccc caggactcg gctccgact cgtcggccg ctgccaaca tcgccgccg ccaatattg ccaaccttg ccatgcgcc tcggcgtca cacaggcca atcaggtga ataacagca gcaacccct caacgcccc ccatcaaca atgacactc tttccgcgt ccatgaacg cgatgaacg ccatgcggg cgtccatgg acagcataa gaaacatgg acgcgatgc ggtcgtcgg tcatggaca tgtcgtcgc ctcagcagt cccagcagc agcaggacg cgctcagga tgcagcagc acgccgagg cgctgctgc ggtcgcagg ccgaggcgg cgctcagac tggccatga gccaggcgc tgccccagc tgaaccagc accagcagg acaacggca gccccctgc ggcacaacg gcaactatc agccgcacc aagggcagt cgtcgcagc agctgagtc cggacctga ccgaggccc tgcggctgc aggagcagc ggctggagc aggccctgc ggctgcacg ggagcgacc cccggactc tgggcttct ccctcagca gccagcagc agaaccaac agccatgaa actttaact actactgaa cgcaaaagt acaatcggc cgccatctt ccattcagc ggccatctt ccagcacgg acgccatat tcgaaaatg tgccaaatt tttgatgcg ctcagtata ctactaatg gccaagcca ttactaaac gcactttac aagtgcacc aactacaca aatcaagag gagaaatca tttaaacaa tgtatagta actaagtgt tggagataa atacctaaa gtaagtgtt tttaggtaa cgttaagta aagcaaata cttaatgca taactatat ttaaggata agctgtaaa tataaagat tgttttaga agaagaaat tcggaatga atgatagag aatttgtaa ttataaagc gcgatgtaa gtacgaggt tttatttac aaaaaatgc gaaaagaat caaatttca cgaggaaaa acttttttt gcaaactgt gattttagt tatttggcc ggttataaa cacgctagc aataaatcg aggaaaaat ca
- >TC030900 RB
ccggagtgc acaggtgat ccgattttt gtgaggttc aagtggcat aatgtggct caaagtgac cgatgattg gaattgagt aaagcgatc ggtaccaat ccggactcg tatttcgtc atcgtctcc gaacccctt taccacgac gccaataac ccagccatc gcgctcgcc acaccgtca accggccac cacatccac ccctagtcc catcgtcgg tactagagc aaaattagt gccgttcga gcgttggct gcgccttct tcttccgtc aaataaagt cgtcgtccc tcacgggcc gtattctcg ccttttccc gccccgccg tcaacattt tcaacacgg aggaccaaa atctaatca acaatctca aggtcgaac atgaaaacc tcgtagcga agtggctgc agaactcat tcaaggccc aggagaacg ccgcactgg acctccggt cgacgccgc tgtcgctgc agataccga tggccgagg tcaacaaca ggcccgtct tcaagatga acaacgact tctggaacc aggcccgcg gcccgcccc agagcatgg cccagaact tgtccaact cgaaccaga actcgccgg tgccgggct ccaccaact ccatcccgg cccactcgc catcgcagc agagcaaca gcagccaaa accacgtca tacaacaac aaccacaaa accaacaac aaccccaac aacaacaac aacaacaaa actcggtac agcaacaaa acaacggcc aacaacaac agcagcaac aacagcaac cgaatcaac aacagcaac agcaacagc aacagcagc aactgggca gtcccaact cgcaggtgg cgcaggccc atgcccatg ccctggccc aggtccagg cccaacagc agttggtgc acatgtcac aacagcacc aactcaacg ctgggcaaa attcgccgg acaaaatgg ccgaaaagt tggtcaatg atttgcaga ttttcatgc acaaatctg cgatgcaaa tgcatgcca gggagctga cgggacgct cagaagtca aaccacacc agtgccagc aatgtctaa agtctttca gcagcaatc accagttag tccaacata tcagggtgc acacggggg aaaaaccct acaaatgtt cctattgtg acaggaggt tcaagcagt tgagtcacg tgcaacagc acacgaggc tacatacgg gtgagaggc cctacaagt gccacttgc ctgactgcg gaagggcct ttatccaac tgtccaacc ttcagcagc atctcagaa accacgacg ctcaggtcg aaagggcca aaaacaggc ccttccact gcaccatct gtggaaaag ggttcgcca ccgagtcca gtctaagga cccatacgt cgaaagtaa gccatcaac atgcagctc tgattggtg gacccaacg ccacttctt gtccgcttt gccataaga tgttcttag gaggagagg cgctcatgg agcacatga agcacacac acaaggacc ccaatgcct cgggggttg ccacgaaga gaaggacag cgaatcatc cttgtcccg tttgtggaa agcattatg tgaatgaag gatccctca ggaaacatt tggcgtgcc atcccgaaa cgtcacaat tgtcaagtt cgttgagaa tgtggccgt gttctgttt gtcaagcag tttttacgc acgaatcag gtcttctca cgcacatgg agcacatga ggatggaca ccaagcacc agttcgccg cccaatatg tgctgtcac gcgccgccg ccgaaagga gagaacgag agactttgc tggcggccg tttccgctt ccgcctccg ggagcggcc ttctaggcc tcacaggaa tggccgggg cagcgagcg gtagcccaa tgtgtgcat cccccagcg ctcacagtg acagttcct caggcaatg gccgccttt cctctgccg gttcagaag ccggcggtc ttaacaaca acaacaaca attgtacaa caaaactga acgagattt tacggagca acaacggtc tccaccaac agtacaacg tcgaagaac agtttcata atgctaaca gaatggccg tcattgcca acatggtgg gccaagggg gggttcccc caggactcg gctccgact cgtcggccg ctgccaaca tcgccgccg ccaatattg ccaaccttg ccatgcgcc tcggcgtca cacaggcca atcaggtga ataacagca gcaacccct caacgcccc ccatcaaca atgacactc tttccgcgt ccatgaacg cgatgaacg ccatgcggg cgtccatgg acagcataa gaaacatgg acgcgatgc ggtcgtcgg tcatggaca tgtcgtcgc ctcagcagt cccagcagc agcaggacg cgctcagga tgcagcagc acgccgagg cgctgctgc ggtcgcagg ccgaggcgg cgctcagac tggccatga gccaggcgc tgccccagc tgaaccagc accagcagg acaacggca gccccctgc ggcacaacg gcaactatc agccgcacc aagggcagt cgtcgcagc agctgagtc cggacctga ccgaggccc tgcggctgc aggagcagc ggctggagc aggccctgc ggctgcacg ggagcgacc cccggactc tgggcttct ccctcagca gccagcagc agaaccaac agccatgaa actttaact actactgaa cgcaaaagt acaatcggc cgccatctt ccattcagc ggccatctt ccagcacgg acgccatat tcgaaaatg tgccaaatt tttgatgcg ctcagtata ctactaatg gccaagcca ttactaaac gcactttac aagtgcacc aactacaca aatcaagag gagaaatca tttaaacaa tgtatagta actaagtgt tggagataa atacctaaa gtaagtgtt tttaggtaa cgttaagta aagcaaata cttaatgca taactatat ttaaggata agctgtaaa tataaagat tgttttaga agaagaaat tcggaatga atgatagag aatttgtaa ttataaagc gcgatgtaa gtacgaggt tttatttac aaaaaatgc gaaaagaat caaatttca cgaggaaaa acttttttt gcaaactgt gattttagt tatttggcc ggttataaa cacgctagc aataaatcg aggaaaaat ca
- >TC030900 RC
ccggagtgc acaggtgat ccgattttt gtgaggttc aagtggcat aatgtggct caaagtgac cgatgattg gaattgagt aaagcgatc ggtaccaat ccggactcg tatttcgtc atcgtctcc gaacccctt taccacgac gccaataac ccagccatc gcgctcgcc acaccgtca accggccac cacatccac ccctagtcc catcgtcgg tactagagc aaaattagt gccgttcga gcgttggct gcgccttct tcttccgtc aaataaagt cgtcgtccc tcacgggcc gtattctcg ccttttccc gccccgccg tcaacattt tcaacacgg aggaccaaa atctaatca acaatctca aggtcgaac atgaaaacc tcgtagcga agtggctgc agaactcat tcaaggccc aggagaacg ccgcactgg acctccggt cgacgccgc tgtcgctgc agataccga tggccgagg tcaacaaca ggcccgtct tcaagatga acaacgact tctggaacc aggcccgcg gcccgcccc agagcatgg cccagaact tgtccaact cgaaccaga actcgccgg tgccgggct ccaccaact ccatcccgg cccactcgc catcgcagc agagcaaca gcagccaaa accacgtca tacaacaac aaccacaaa accaacaac aaccccaac aacaacaac aacaacaaa actcggtac agcaacaaa acaacggcc aacaacaac agcagcaac aacagcaac cgaatcaac aacagcaac agcaacagc aacagcagc aactgggca gtcccaact cgcaggtgg cgcaggccc atgcccatg ccctggccc aggtccagg cccaacagc agttggtgc acatgtcac aacagcacc aactcaacg ctgggcaaa attcgccgg acaaaatgg ccgaaaagt tggtcaatg atttgcagt tgctttccc aactaacgc tgccggact ttaaccaac tgcagagga attcgactt cttcggcca tctccgaaa ggacgcttg aagaatgtt ggaccacac tgcagcgaa ttttcatgc acaaatctg cgatgcaaa tgcatgcca gggagctga cgggacgct cagaagtca aaccacacc agtgccagc aatgtctaa agtctttca gcagcaatc accagttag tccaacata tcagggtgc acacggggg aaaaaccct acaaatgtt cctattgtg acaggaggt tcaagcagt tgagtcacg tgcaacagc acacgaggc tacatacgg gtgagaggc cctacaagt gccacttgc ctgactgcg gaagggcct ttatccaac tgtccaacc ttcagcagc atctcagaa accacgacg ctcaggtcg aaagggcca aaaacaggc ccttccact gcaccatct gtggaaaag ggttcgcca ccgagtcca gtctaagga cccatacgt cgaaagtaa gccattgta ttggtcgtt tgcagcaac atgcagctc tgattggtg gacccaacg ccacttctt gtccgcttt gccataaga tgttcttag gaggagagg cgctcatgg agcacatga agcacacac acaaggacc ccaatgcct cgggggttg ccacgaaga gaaggacag cgaatcatc cttgtcccg tttgtggaa agcattatg tgaatgaag gatccctca ggaaacatt tggcgtgcc atcccgaaa cgtcacaat tgtcaagtt cgttgagaa tgtggccgt gttctgttt gtcaagcag tttttacgc acgaatcag gtcttctca cgcacatgg agcacatga ggatggaca ccaagcacc agttcgccg cccaatatg tgctgtcac gcgccgccg ccgaaagga gagaacgag agactttgc tggcggccg tttccgctt ccgcctccg ggagcggcc ttctaggcc tcacaggaa tggccgggg cagcgagcg gtagcccaa tgtgtgcat cccccagcg ctcacagtg acagttcct caggcaatg gccgccttt cctctgccg gttcagaag ccggcggtc ttaacaaca acaacaaca attgtacaa caaaactga acgagattt tacggagca acaacggtc tccaccaac agtacaacg tcgaagaac agtttcata atgctaaca gaatggccg tcattgcca acatggtgg gccaagggg gggttcccc caggactcg gctccgact cgtcggccg ctgccaaca tcgccgccg ccaatattg ccaaccttg ccatgcgcc tcggcgtca cacaggcca atcaggtga ataacagca gcaacccct caacgcccc ccatcaaca atgacactc tttccgcgt ccatgaacg cgatgaacg ccatgcggg cgtccatgg acagcataa gaaacatgg acgcgatgc ggtcgtcgg tcatggaca tgtcgtcgc ctcagcagt cccagcagc agcaggacg cgctcagga tgcagcagc acgccgagg cgctgctgc ggtcgcagg ccgaggcgg cgctcagac tggccatga gccaggcgc tgccccagc tgaaccagc accagcagg acaacggca gccccctgc ggcacaacg gcaactatc agccgcacc aagggcagt cgtcgcagc agctgagtc cggacctga ccgaggccc tgcggctgc aggagcagc ggctggagc aggccctgc ggctgcacg ggagcgacc cccggactc tgggcttct ccctcagca gccagcagc agaaccaac agccatgaa actttaact actactgaa cgcaaaagt acaatcggc cgccatctt ccattcagc ggccatctt ccagcacgg acgccatat tcgaaaatg tgccaaatt tttgatgcg ctcagtata ctactaatg gccaagcca ttactaaac gcactttac aagtgcacc aactacaca aatcaagag gagaaatca tttaaacaa tgtatagta actaagtgt tggagataa atacctaaa gtaagtgtt tttaggtaa cgttaagta aagcaaata cttaatgca taactatat ttaaggata agctgtaaa tataaagat tgttttaga agaagaaat tcggaatga atgatagag aatttgtaa ttataaagc gcgatgtaa gtacgaggt tttatttac aaaaaatgc gaaaagaat caaatttca cgaggaaaa acttttttt gcaaactgt gattttagt tatttggcc ggttataaa cacgctagc aataaatcg aggaaaaat ca
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