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Gene information
General information
TC015522 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1714727..1719366
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015522
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05833

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015522 RA
      MLTSRVLLG LGSRALRRG FASDAKAEV TALYDFHVE 
NGGKMVNFG GFMLPVQYS GLGIAASHL HTRKNASLF 
DVSHMLQTE ISGADCLSY MESICTADL KTLPPNTST 
LTVFTNDKG GVLDDLIIT KISDDHLYV VSNAAMKKQ 
DQQHLLTAL DSHKKTNPN SNIKMKFFE PSERGLVAL 
QGPKAAEVL QKLTDVDLS KLYFMTSTE ATVCGCGAC 
RVTRCGYTG EDGFEISMP AIKQVDITR EIMKNEAVK 
LAGLGARDS LRLEAGMCL YGNDLTAET TPIEAALTW 
LVAKRRRES RDFPGAETI VSQIKNGTS RKRVGLIAD 
SGPPARHGT PIVDANGNE IGSVTSGCP SPSLGKNIA 
MAYVPADLS KNGTKHNLK IRDKIYSAV VTKMPFVPS 
NYYNKPK
    CDS sequence
    • >TC015522 RA
      atgttgacg tcgagagtg ttgctgggt ttggggtcg 
cgggcgctc cgcaggggc tttgccagc gatgcgaag 
gccgaagtt acggccctc tacgatttt cacgtggaa 
aatgggggc aaaatggtc aattttggc gggtttatg 
ctcccggtg cagtacagc ggtttgggc atagccgcc 
tcacatctg cacaccagg aaaaatgcg tcgcttttc 
gacgtctcc catatgcta cagacggaa atctccggc 
gctgactgc ttaagttac atggagtct atctgtacc 
gccgacctc aaaaccctc ccccctaac acctccacc 
ctcaccgtc ttcaccaac gacaaaggg ggcgtgctt 
gacgacttg atcatcaca aaaatttcc gatgatcac 
ttatacgtt gtttcaaat gccgccatg aaaaaacaa 
gatcaacaa cacttgctc acagccctc gacagccac 
aaaaaaaca aaccccaac tccaacatc aagatgaag 
tttttcgaa ccaagcgaa cgcggattg gttgcttta 
caaggcccg aaggcggct gaggttcta caaaaactg 
acagatgtc gacctgtca aaactatat ttcatgaca 
tcaacggag gccacggtt tgtggctgt ggcgcatgc 
cgggtcacc cggtgtggt tacactggc gaagatggc 
tttgaaatt tcaatgccg gccataaaa caagtcgac 
atcacgcga gaaatcatg aaaaatgag gcggtcaag 
ttggctggg ttgggtgca agggattcg ctacgcctt 
gaagctggg atgtgtctc tatggtaac gacctgaca 
gccgagacc acgcccatc gaagccgcc ttgacgtgg 
ctcgttgcc aaacgtcgc cgcgagtca cgtgacttt 
ccaggggcg gaaactatc gtttcgcag attaaaaat 
ggaacaagt aggaaaagg gtcggactg attgcggat 
tcgggcccc ccagctcgt cacggcacc cccattgtg 
gacgccaat ggcaatgaa atcggttcg gttacgtcc 
ggttgtcct tcgccaagt ctcggcaaa aacatcgca 
atggcgtac gtcccagcc gacttgagc aaaaacgga 
acgaaacat aatctcaaa atacgcgat aaaatatac 
tcagctgtt gtgactaaa atgccgttt gttccctca 
aactactac aataaaccg aaataa
    mRNA sequence
    • >TC015522 RA
      tttgttttc ggctttgtt agtttccta tttccggga 
ctgtcccgc actttacgt gcggccttt ctaccggtt 
catgtggcc acagggatt gcgactttc acgctttgc 
gtggttacc gcattggtg ggaatcacc gaaaaaaca 
atttggaca ctgggtagt aaataccaa gatttgccg 
tcagaggga gttatggtg aatttcatc ggccttttt 
atcgcattt tgggggatt ttggtgctt tatttggtc 
aacgatgtt gactttaag agggctaat ctaccggaa 
gacgaggtc gcattgtca aactctaac acggattaa 
gcagtggtg ccaatacag ttggcacga ctgcgtcct 
attgattta attattatg gcggaaaaa agccatgca 
ttttcattt ggtgctaaa atttggtct caatattcg 
aattatgtt gacttgaat gtgttatcg cgttgatta 
aacttagta agtaattag caacgtcac ttataccaa 
tatatacta tacataatt acgatatac ttaatatat 
aaatgtatg tttatgtgg aagcaaaaa tgtttgttt 
tatttcccc attatcgag tgacctcag ctcagcttc 
agagccgac tccgataaa aatcctcag tcgtggtca 
tgttgacgt cgagagtgt tgctgggtt tggggtcgc 
gggcgctcc gcaggggct ttgccagcg atgcgaagg 
ccgaagtta cggccctct acgattttc acgtggaaa 
atgggggca aaatggtca attttggcg ggtttatgc 
tcccggtgc agtacagcg gtttgggca tagccgcct 
cacatctgc acaccagga aaaatgcgt cgcttttcg 
acgtctccc atatgctac agacggaaa tctccggcg 
ctgactgct taagttaca tggagtcta tctgtaccg 
ccgacctca aaaccctcc cccctaaca cctccaccc 
tcaccgtct tcaccaacg acaaagggg gcgtgcttg 
acgacttga tcatcacaa aaatttccg atgatcact 
tatacgttg tttcaaatg ccgccatga aaaaacaag 
atcaacaac acttgctca cagccctcg acagccaca 
aaaaaacaa accccaact ccaacatca agatgaagt 
ttttcgaac caagcgaac gcggattgg ttgctttac 
aaggcccga aggcggctg aggttctac aaaaactga 
cagatgtcg acctgtcaa aactatatt tcatgacat 
caacggagg ccacggttt gtggctgtg gcgcatgcc 
gggtcaccc ggtgtggtt acactggcg aagatggct 
ttgaaattt caatgccgg ccataaaac aagtcgaca 
tcacgcgag aaatcatga aaaatgagg cggtcaagt 
tggctgggt tgggtgcaa gggattcgc tacgccttg 
aagctggga tgtgtctct atggtaacg acctgacag 
ccgagacca cgcccatcg aagccgcct tgacgtggc 
tcgttgcca aacgtcgcc gcgagtcac gtgactttc 
caggggcgg aaactatcg tttcgcaga ttaaaaatg 
gaacaagta ggaaaaggg tcggactga ttgcggatt 
cgggccccc cagctcgtc acggcaccc ccattgtgg 
acgccaatg gcaatgaaa tcggttcgg ttacgtccg 
gttgtcctt cgccaagtc tcggcaaaa acatcgcaa 
tggcgtacg tcccagccg acttgagca aaaacggaa 
cgaaacata atctcaaaa tacgcgata aaatatact 
cagctgttg tgactaaaa tgccgtttg ttccctcaa 
actactaca ataaaccga aataaatga tttgaaatg 
atacgagtt tgaatttgg cgccccatc cccactcct 
tgacgttga aagaagtga aaataacac cgtttttgt 
ggttttgtt aaaggaatt acattcaaa agcaagcta 
aataaatta aattttttg aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY