Status
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Gene information
General information
TC033354
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015519,TC015518
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1689040..1705714
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033354
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05832

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Many different defects known in the background.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033354 RA
      MATANPDET DKDRYGLGY QNTVVSILV PNRSSWRGK 
      IDVKSLPAM RSRYRRPAR DLKARDGKV TGPRRAALP 
      HLCVFGIMT EMSEAHFGE ADPESVDKE WEEFAKLVE 
      KRKKRREQS FYPCPPEED YVSTLMYGR YSRQLGEFA 
      KLEGLRQAE KRRLKEDRA RKKELRGYQ GKLAKAVSF 
      LWKHTFARL GEDWVFLAL LGVIMAVLS FIMDRGISL 
      CNWARIWLY NDLNGQPFA QYAAWVSLP VCLILFSAG 
      FVHLVAPQS IGSGIPEMK TILRGVALK EYLTFRTLV 
      AKVIGLTAT LGSGLPLGK EGPFVHIAS ISATLLSKL 
      VTGFQGIYE NENRTTEML AAACAVGVG SCFAAPVGG 
      VLFSIEVTT VYFAVRNYW RGFFAAVCG ATIFRLLAV 
      WFDRADTVT AVFKTSFQM DFPFDPQEL FVFALIGAF 
      CGLGGAFYV WMHRQYVMF MRNSKTINS FLQKNRFLY 
      PAIVSLLVS TVSFPLGTG QYFAGDLTT HEQVMDLFS 
      NFTWTTDNL SADQHNVLK HWQTPHTGV FVSLISYVA 
      FTFIFSIIC STIPVPSGS FIPVFKIGA GFGRLVGEL 
      MHMWFPSGM RYGGQIATI IPGGYATVG AAAFSGAVT 
      HTISVSVIV FEMTGQISH IIPIMIAVL ISNAIASLL 
      APSIYDSII LIKKLPYLP DLLPSSSGM YKVYVEDFM 
      VRDVRYIFN NMTYDQLKT LLKENRKLQ SFPLVDNHD 
      NMILLGSIQ RLQLIQLIE KHIGRERRL QVAAIRQRE 
      AEERAREEA ERKAEERKR RPSRFQVVP APDVLGVKQ 
      MSENQLNVD KPTFHPVFG SQPKKSILK KTNSFTLKG 
      FSPFISTSP GSTPYTTVT GAESRIRLA FEAIFRKSA 
      QLQDVENND SSPVLNSAG GSMDNKVQI IPMSPSTSK 
      KVQLPKERV CDMSPEDQR IWEEEQMQL PVDFTSCHI 
      DPAPFQLVE KTSLLKVHS LFSMVGVNH AYVTAIGKL 
      VGVVGLKDL RKAIEDANS GNLPRQSVE TNSNGNVGT 
      IGDNGDLDN EAAQALVSK V
    CDS sequence
    • >TC033354 RA
      atggcgacc gcaaatcct gatgaaacc gacaaggat 
      cgctatgga ttgggatac cagaataca gtggtgagt 
      attttagtg ccaaatcga tctagttgg cgtggaaaa 
      attgacgtg aaatcgcta ccggcaatg cgatcgcga 
      taccggaga ccggcacgt gatcttaag gcacgtgat 
      gggaaggtc acgggccca aggagggcc gcactccca 
      cacctttgc gtctttggc atcatgacc gaaatgagc 
      gaagcccat ttcggggaa gccgatccc gaatcggtc 
      gataaagaa tgggaagag ttcgcgaaa ttggtggaa 
      aagcggaaa aagcgacgg gagcagagc ttctacccg 
      tgccccccc gaagaggat tacgtcagc actttaatg 
      tatggccga tacagtcgg caattgggc gagtttgcg 
      aagctagaa gggctcaga caggccgaa aagaggcga 
      ctgaaggag gatagggcc cgcaaaaag gagctgagg 
      ggctatcaa ggaaagctc gccaaggcc gtgtcgttc 
      ctatggaag cacactttc gcgcgcttg ggcgaggac 
      tgggtgttt ttggcgctg ttgggggtc ataatggcc 
      gtgctcagc tttataatg gatcggggg atttccctg 
      tgtaattgg gcgcgaatt tggctttac aatgattta 
      aatggacag ccgtttgcg caatatgcg gcttgggtg 
      tctttgccg gtttgtttg attttgttt agtgctggg 
      tttgtgcat ttggtggcg ccgcagtcg ataggttcg 
      gggattccc gaaatgaag acaatcctc cgcggcgtc 
      gccctcaaa gaatactta acgttccgt actctcgtc 
      gccaaagta atcggcctc accgccacc ctcggcagc 
      ggccttccc ctgggcaaa gagggccct ttcgtccac 
      atcgccagc atttctgcc actcttttg agcaaactc 
      gtgaccggc tttcagggc atttacgaa aacgagaac 
      cgcaccact gaaatgctc gccgccgcc tgcgccgtg 
      ggggtgggc agctgcttc gccgccccc gtgggcggc 
      gtcctcttc agcatcgag gtcacgacg gtctacttc 
      gccgtccgc aactactgg cgcggtttt ttcgccgcc 
      gtctgcggc gccaccatc ttccgcctg ttggcggtt 
      tggttcgac agggctgac accgtcacc gccgtgttc 
      aagaccagc ttccagatg gacttcccg ttcgaccct 
      caggagctg ttcgttttc gccctgatt ggggcgttc 
      tgcgggctg gggggcgcg ttctacgtg tggatgcac 
      cgccaatac gtcatgttt atgcgaaat agcaaaacc 
      attaatagt ttcttgcag aaaaatcgt ttcctgtat 
      cctgccatt gtgagtctt ctagtctcg actgtttcg 
      tttccgtta gggacggga cagtatttt gcgggcgat 
      ttgacaacg catgaacaa gtcatggat ttgtttagc 
      aattttacg tggactacc gataattta tcggctgat 
      cagcataac gttttgaag cattggcaa acgccgcac 
      actggggtc ttcgtcagc ttgatttcc tatgtggcg 
      tttacgttc atcttcagc atcatctgc tccaccatc 
      cccgtcccc agcggcagt ttcatcccc gtttttaaa 
      attggcgcc ggtttcggc cgcctcgtg ggcgaactg 
      atgcacatg tggttcccc agcgggatg cgctacggg 
      ggccaaatc gccaccatc atccccggg ggctacgcc 
      accgtgggg gcggccgcc ttcagcggc gccgtcacc 
      cacaccatc tccgtctcc gtcatagtt ttcgaaatg 
      acaggacaa atatcgcac atcatccca atcatgatc 
      gccgtttta atatcaaac gcaatcgct agtcttcta 
      gcccccagc atttacgac tcgattatt ttgataaaa 
      aaattgccc tatttgccc gacctgttg cccagcagc 
      agcggcatg tacaaagtg tacgtggag gattttatg 
      gtgcgtgat gttcgctac attttcaat aacatgacc 
      tacgaccaa ttaaaaaca ttgctgaag gagaatcgc 
      aaattgcaa agcttccca ttggttgat aaccacgat 
      aacatgatc ttgcttggc tcaattcaa cgcttgcaa 
      ttgatccaa ttgatcgag aaacacatc gggagagag 
      agaagattg caagtggcg gcgatcaga cagagagag 
      gccgaggag cgggcaagg gaggaggcg gagcgtaag 
      gcggaggaa cgcaaacgt cgcccctcc cgctttcaa 
      gttgtcccc gcccctgac gtactgggc gttaagcaa 
      atgtcggag aatcaactc aatgttgat aaaccgacg 
      tttcatcct gtttttggg tcacaaccc aaaaaatca 
      atcttgaaa aaaaccaat tcctttacg ttgaaaggt 
      ttcagtccg tttatttct acaagtcca ggctctact 
      ccgtacacg actgttacg ggcgctgaa agtcggatt 
      cgattggcg tttgaagcg atttttcga aaatcggct 
      caacttcaa gatgttgag aataatgac agttcgcca 
      gtgttgaat agtgcagga ggttcgatg gataataag 
      gtgcaaatt ataccgatg agtccgtca acatccaag 
      aaggtgcaa ttgcccaag gagcgcgtg tgcgacatg 
      tcccccgag gaccagcgc atctgggag gaggagcaa 
      atgcagctc cctgtcgac tttacttcg tgtcacatc 
      gatccagcg ccattccaa ttggtggag aaaacgtct 
      ctgctcaaa gtccactcc ttgttttca atggtgggg 
      gtaaaccac gcctatgtg acagccatt ggcaagttg 
      gtgggcgtg gtcggtcta aaagacctt cgtaaagcc 
      atcgaagat gccaatagt ggtaatcta ccacgtcaa 
      agtgtcgaa acgaactcg aatggtaat gttggtacc 
      attggtgat aacggtgat ttggacaat gaggcggct 
      caagcgctc gtctcgaag gtctag
    mRNA sequence
    • >TC033354 RA
      ttgaatttg aacaatttc ccgaaaatt gtgtttatt 
      tcgctccag tagcacgtg aatcggcac ctgattggc 
      gaacttgtc gcgtgtgtc caatcaatt ggcacattt 
      gggccgaga ttgttgatt ttgaagtgc taacaagta 
      ctccacagt gttttctaa ttaacaatt ttatctcat 
      tttttgaca tagttttct tcacatata tcacaagtt 
      tgaagcagt tgttatcac agtgtcggt gcgtttgca 
      ttgtttcac ccatccatg ggttaattg aactggtac 
      gactgcaca ggaagttcg caacgaaga agaagaaga 
      agaaaaaat ggcgaccgc aaatcctga tgaaaccga 
      caaggatcg ctatggatt gggatacca gaatacagt 
      ggtgagtat tttagtgcc aaatcgatc tagttggcg 
      tggaaaaat tgacgtgaa atcgctacc ggcaatgcg 
      atcgcgata ccggagacc ggcacgtga tcttaaggc 
      acgtgatgg gaaggtcac gggcccaag gagggccgc 
      actcccaca cctttgcgt ctttggcat catgaccga 
      aatgagcga agcccattt cggggaagc cgatcccga 
      atcggtcga taaagaatg ggaagagtt cgcgaaatt 
      ggtggaaaa gcggaaaaa gcgacggga gcagagctt 
      ctacccgtg cccccccga agaggatta cgtcagcac 
      tttaatgta tggccgata cagtcggca attgggcga 
      gtttgcgaa gctagaagg gctcagaca ggccgaaaa 
      gaggcgact gaaggagga tagggcccg caaaaagga 
      gctgagggg ctatcaagg aaagctcgc caaggccgt 
      gtcgttcct atggaagca cactttcgc gcgcttggg 
      cgaggactg ggtgttttt ggcgctgtt gggggtcat 
      aatggccgt gctcagctt tataatgga tcgggggat 
      ttccctgtg taattgggc gcgaatttg gctttacaa 
      tgatttaaa tggacagcc gtttgcgca atatgcggc 
      ttgggtgtc tttgccggt ttgtttgat tttgtttag 
      tgctgggtt tgtgcattt ggtggcgcc gcagtcgat 
      aggttcggg gattcccga aatgaagac aatcctccg 
      cggcgtcgc cctcaaaga atacttaac gttccgtac 
      tctcgtcgc caaagtaat cggcctcac cgccaccct 
      cggcagcgg ccttcccct gggcaaaga gggcccttt 
      cgtccacat cgccagcat ttctgccac tcttttgag 
      caaactcgt gaccggctt tcagggcat ttacgaaaa 
      cgagaaccg caccactga aatgctcgc cgccgcctg 
      cgccgtggg ggtgggcag ctgcttcgc cgcccccgt 
      gggcggcgt cctcttcag catcgaggt cacgacggt 
      ctacttcgc cgtccgcaa ctactggcg cggtttttt 
      cgccgccgt ctgcggcgc caccatctt ccgcctgtt 
      ggcggtttg gttcgacag ggctgacac cgtcaccgc 
      cgtgttcaa gaccagctt ccagatgga cttcccgtt 
      cgaccctca ggagctgtt cgttttcgc cctgattgg 
      ggcgttctg cgggctggg gggcgcgtt ctacgtgtg 
      gatgcaccg ccaatacgt catgtttat gcgaaatag 
      caaaaccat taatagttt cttgcagaa aaatcgttt 
      cctgtatcc tgccattgt gagtcttct agtctcgac 
      tgtttcgtt tccgttagg gacgggaca gtattttgc 
      gggcgattt gacaacgca tgaacaagt catggattt 
      gtttagcaa ttttacgtg gactaccga taatttatc 
      ggctgatca gcataacgt tttgaagca ttggcaaac 
      gccgcacac tggggtctt cgtcagctt gatttccta 
      tgtggcgtt tacgttcat cttcagcat catctgctc 
      caccatccc cgtccccag cggcagttt catccccgt 
      ttttaaaat tggcgccgg tttcggccg cctcgtggg 
      cgaactgat gcacatgtg gttccccag cgggatgcg 
      ctacggggg ccaaatcgc caccatcat ccccggggg 
      ctacgccac cgtgggggc ggccgcctt cagcggcgc 
      cgtcaccca caccatctc cgtctccgt catagtttt 
      cgaaatgac aggacaaat atcgcacat catcccaat 
      catgatcgc cgttttaat atcaaacgc aatcgctag 
      tcttctagc ccccagcat ttacgactc gattatttt 
      gataaaaaa attgcccta tttgcccga cctgttgcc 
      cagcagcag cggcatgta caaagtgta cgtggagga 
      ttttatggt gcgtgatgt tcgctacat tttcaataa 
      catgaccta cgaccaatt aaaaacatt gctgaagga 
      gaatcgcaa attgcaaag cttcccatt ggttgataa 
      ccacgataa catgatctt gcttggctc aattcaacg 
      cttgcaatt gatccaatt gatcgagaa acacatcgg 
      gagagagag aagattgca agtggcggc gatcagaca 
      gagagaggc cgaggagcg ggcaaggga ggaggcgga 
      gcgtaaggc ggaggaacg caaacgtcg cccctcccg 
      ctttcaagt tgtccccgc ccctgacgt actgggcgt 
      taagcaaat gtcggagaa tcaactcaa tgttgataa 
      accgacgtt tcatcctgt ttttgggtc acaacccaa 
      aaaatcaat cttgaaaaa aaccaattc ctttacgtt 
      gaaaggttt cagtccgtt tatttctac aagtccagg 
      ctctactcc gtacacgac tgttacggg cgctgaaag 
      tcggattcg attggcgtt tgaagcgat ttttcgaaa 
      atcggctca acttcaaga tgttgagaa taatgacag 
      ttcgccagt gttgaatag tgcaggagg ttcgatgga 
      taataaggt gcaaattat accgatgag tccgtcaac 
      atccaagaa ggtgcaatt gcccaagga gcgcgtgtg 
      cgacatgtc ccccgagga ccagcgcat ctgggagga 
      ggagcaaat gcagctccc tgtcgactt tacttcgtg 
      tcacatcga tccagcgcc attccaatt ggtggagaa 
      aacgtctct gctcaaagt ccactcctt gttttcaat 
      ggtgggggt aaaccacgc ctatgtgac agccattgg 
      caagttggt gggcgtggt cggtctaaa agaccttcg 
      taaagccat cgaagatgc caatagtgg taatctacc 
      acgtcaaag tgtcgaaac gaactcgaa tggtaatgt 
      tggtaccat tggtgataa cggtgattt ggacaatga 
      ggcggctca agcgctcgt ctcgaaggt ctagtgata 
      aggcagcga gagtgattt ttatgtgat aaaacagcg 
      atttattta attattgaa tgaaaatgt taaaaaaat 
      aaagggtat gatgaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY