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Gene information
General information
TC015502 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1572279..1589253
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015502
    iBeetle screen iB_05828

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015502 RA
      MPVRRGLVA PRTTLIETI IRKFDTDNR SFLVANSRE 
      GQCHIIYCS DGFCRLTGY SRAEVMQRS ASCDFLCGP 
      LTSQQAVGS LREALQEGV EKHAEILYY RKDGSKFLC 
      SEVIAPIRS EVDDISLII INFEDLTAV PTASLESSP 
      VGKPRLSKF DRARASFRQ SLRLGSNLR GRGLRLAGY 
      LTPPSDVTA AEEEADTLE QCPLTSNSP TPILEQTWV 
      SKTEVPTAT THTTIALPS QTKEYTALA ISHSAPTSH 
      QASFERGES VERSRPPIT RHQTPNRVS HATSLDAIA 
      RRQCFRTGY PTVSVSNKP HLSKQFPNV SSESDLQRY 
      RTAPQSHDR KSPSLSNPT DSSKQKVCP QFSFQDNGS 
      AKYFQSGIH TPKMGEKVA QCVKCDGIM LLKNQPMDD 
      INPENITII KKIIDDFEQ NPGKVPKKR RCRSCKISQ 
      SDSLIHRYR RCQISPKSG SLDGAFFRV RCRGTSLEN 
      FIRSEDEVY INFAENGDD NGNADANAF KPRVRRSFL 
      KANNVGVDE VKAYMESKK HQERVTKNV KENEFQREK 
      LN
    CDS sequence
    • >TC015502 RA
      atgccggtg cgaagaggg cttgtcgct cccaggacc 
      acgcttatt gaaacaatc atcaggaaa ttcgacact 
      gacaatcgt tcatttttg gtggccaac agtcgcgaa 
      ggacaatgt catataatc tactgttcg gacggcttc 
      tgtcgactg acgggctat tccagagcg gaagtgatg 
      caacgctcg gcctcctgc gacttcttg tgcggccct 
      ttgacctcg caacaggcc gtcggcagt ctcagagag 
      gcccttcaa gaaggcgtc gagaaacac gcagagata 
      ctatattac aggaaagat ggctcaaag ttcctatgt 
      tccgaagta atcgccccc atacggagc gaagtggat 
      gacataagt ttgattatc atcaatttc gaagactta 
      acggcggtt ccgacggcg tctctggaa agcagtccc 
      gtggggaaa ccccgactt agcaaattt gacagggcc 
      agagcctcg ttcagacag agtctcagg ttgggttcg 
      aatttgcga ggaagaggc cttagacta gcggggtat 
      ttgacgccc ccgagtgac gtaaccgcc gccgaagag 
      gaggcggat actttggag caatgtcct ctgacatcg 
      aattctccg actcccatt ctggaacag acttgggtg 
      tcgaagacc gaagtgccg acggctacg acgcacaca 
      acgattgca ctgccgtct cagaccaaa gaatacaca 
      gctttggcg atatcacat agtgcgcca acgagccac 
      caagcctcg tttgaacgt ggagaaagt gtggaaaga 
      agtagacca ccgatcact aggcaccaa acaccaaat 
      agagtctcg catgcgaca agtttagat gctatagct 
      aggaggcag tgttttagg acaggatat cccactgtt 
      agtgtttcc aataaaccg cacttgagc aagcaattt 
      cccaacgtt tcgtccgag agcgattta caacgctac 
      agaacggcc ccacaatca cacgatcgc aaaagtcca 
      agtctgagc aaccccacc gattcgtca aaacagaaa 
      gtctgtccc cagttctcg ttccaagac aatggatcg 
      gcgaaatac ttccaaagt gggattcac acgccgaaa 
      atgggcgaa aaagtggct cagtgtgta aaatgtgac 
      ggcatcatg ttgctcaaa aaccaacca atggacgac 
      atcaatcca gaaaacatc accatcatc aagaaaatc 
      atcgacgat ttcgaacag aatccgggg aaagtcccg 
      aaaaagcgg cgttgccgc agctgcaaa atcagtcaa 
      agcgattcg ttgatacat cggtatcgg cgttgccag 
      atttcgcca aaatccggg agtttggac ggggcgttt 
      tttcgggtg aggtgtcgg gggacgagt ttggagaat 
      tttattcgg agtgaggat gaggtgtat attaatttc 
      gcggaaaat ggggatgat aatggcaac gccgacgcg 
      aatgcgttt aaaccgaga gttaggagg agctttctt 
      aaggcgaat aatgtgggg gtggacgaa gttaaggcc 
      tatatggag tcgaagaag caccaggaa agggtgacc 
      aagaatgtc aaagagaac gagttccag agggaaaaa 
      ttgaactaa
    mRNA sequence
    • >TC015502 RA
      tcagttgaa tgacactga gcttcgagg cgcgccaat 
      gtcgcgaaa atctcgagt gcaatgcgc gcgcaccct 
      cgggggtgt atttttacc gaaattcgt atagatttg 
      gtcgtcttt tggtgaaaa tcgccggat ttttcgtgc 
      aaggcgttt atttttaaa gcgctacaa atagccgcg 
      attttatat ttaaaccgc tgaatttgc cttatggat 
      gccttattt ttggtgttt ctatgaatt tttcctaac 
      gaaaaaatg atcatcgag tgtgcattg tagttgttg 
      atatgccgg tgcgaagag ggcttgtcg ctcccagga 
      ccacgctta ttgaaacaa tcatcagga aattcgaca 
      ctgacaatc gttcatttt tggtggcca acagtcgcg 
      aaggacaat gtcatataa tctactgtt cggacggct 
      tctgtcgac tgacgggct attccagag cggaagtga 
      tgcaacgct cggcctcct gcgacttct tgtgcggcc 
      ctttgacct cgcaacagg ccgtcggca gtctcagag 
      aggcccttc aagaaggcg tcgagaaac acgcagaga 
      tactatatt acaggaaag atggctcaa agttcctat 
      gttccgaag taatcgccc ccatacgga gcgaagtgg 
      atgacataa gtttgatta tcatcaatt tcgaagact 
      taacggcgg ttccgacgg cgtctctgg aaagcagtc 
      ccgtgggga aaccccgac ttagcaaat ttgacaggg 
      ccagagcct cgttcagac agagtctca ggttgggtt 
      cgaatttgc gaggaagag gccttagac tagcggggt 
      atttgacgc ccccgagtg acgtaaccg ccgccgaag 
      aggaggcgg atactttgg agcaatgtc ctctgacat 
      cgaattctc cgactccca ttctggaac agacttggg 
      tgtcgaaga ccgaagtgc cgacggcta cgacgcaca 
      caacgattg cactgccgt ctcagacca aagaataca 
      cagctttgg cgatatcac atagtgcgc caacgagcc 
      accaagcct cgtttgaac gtggagaaa gtgtggaaa 
      gaagtagac caccgatca ctaggcacc aaacaccaa 
      atagagtct cgcatgcga caagtttag atgctatag 
      ctaggaggc agtgtttta ggacaggat atcccactg 
      ttagtgttt ccaataaac cgcacttga gcaagcaat 
      ttcccaacg tttcgtccg agagcgatt tacaacgct 
      acagaacgg ccccacaat cacacgatc gcaaaagtc 
      caagtctga gcaacccca ccgattcgt caaaacaga 
      aagtctgtc cccagttct cgttccaag acaatggat 
      cggcgaaat acttccaaa gtgggattc acacgccga 
      aaatgggcg aaaaagtgg ctcagtgtg taaaatgtg 
      acggcatca tgttgctca aaaaccaac caatggacg 
      acatcaatc cagaaaaca tcaccatca tcaagaaaa 
      tcatcgacg atttcgaac agaatccgg ggaaagtcc 
      cgaaaaagc ggcgttgcc gcagctgca aaatcagtc 
      aaagcgatt cgttgatac atcggtatc ggcgttgcc 
      agatttcgc caaaatccg ggagtttgg acggggcgt 
      tttttcggg tgaggtgtc gggggacga gtttggaga 
      attttattc ggagtgagg atgaggtgt atattaatt 
      tcgcggaaa atggggatg ataatggca acgccgacg 
      cgaatgcgt ttaaaccga gagttagga ggagctttc 
      ttaaggcga ataatgtgg gggtggacg aagttaagg 
      cctatatgg agtcgaaga agcaccagg aaagggtga 
      ccaagaatg tcaaagaga acgagttcc agagggaaa 
      aattgaact aaccctgaa tttagaaaa attaagttc 
      agaggtcaa caagccttc gctgctcct ttgtaacaa 
      aagttgaga aaacaaaag gattctaat taggacgag 
      gaataaaac gcaacatca aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY