Status
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Gene information
General information
TC030899
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015501,TC015500 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1558636..1564082
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC030899
    iBeetle screen iB_05827

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030899 RA
      MKTKIAILQ TPLTRQILV ATGPLLSTI GLGMTSGFS 
AVLLPQLHN TTLVASPND ASWIASMAA LPMAFGCVF 
GGFLMENFG RKTTQILTT IPSLIGWLL IGFSSDIWM 
ILTGRFLTG LCGGLLGPS TGVYISETS EPRFRGFLL 
ASISLAMAL GLFFVHFLG TFLTWRTTS GLSLILPVL 
SLVVLNLVP ESPSWLAKK GRNDEAQKS FFWCRGESD 
QARKELTEM LQRYKNQTL ETEKIKLIS GEFLKPLAI 
IVVFIVTNQ WAGVNALTF YTVTIMGKT LGPGLDEYL 
AMLVVDLIR VAMSIATCF IMKRIGRRP LALISGFGT 
FTSLFLLSA YSFTTRFVR LDFPLVPMG ALVAYITFI 
TIGFVPLPW AMMGEVFPQ THRNVGSSV SSFMAFVAF 
FSVVKTSPA MFDCLGTDG TFMVYGAVA FFGTIFNWF 
FLPETKDKT LGEIEDEFK YLFLTDGET CLVNCDERE 
MIDCERFAP RVRQTFVAL GPILITTSL GMTEGFSAI 
LLPQLNSTS LQIDEETSS WIASMAALP MALGCILGG 
ILMEKIGRK ATHMLTCLP CVIGWLILY FASSVDMIL 
VGRFLTGFC VGLLGPPTG VYMSETSEP KFRGFLLAS 
ISFAIALGL FLSHLIGTF VNWQDTALT CCSFPVICL 
VFMGFAPES PTWLAKRGR LEEAKRAFV WCRGQSEEA 
VNELEVLIN RQTILNQEE TKSFCEIIK DLKRPEFIK 
PLVIIVVFF VTCQWSGLN AITFYSVTI IQQTLGGNF 
DEYLAMLII DSIRVFMSV LACVLLKKL GRRPLAIIS 
GVGTFVSLF ILSSFTFAV KFYPAISVY TFIPLVSLI 
TYVSFITIG FVPLPWTMM GEVFPLANR GIGSGISAL 
MAYVAFFSV VKTTPAMIQ HFGLEGTFF IYGMLALVG 
TIILILFLP ETKDKALYQ IEDNFKLDC K
    CDS sequence
    • >TC030899 RA
      atgaagacc aaaatcgcc atcctccaa acgccacta 
acccgccaa attctcgta gccaccggc cctttgctc 
tccacaatc ggcctcggc atgacttcg ggcttctcc 
gccgtgctc ttaccccaa ctccacaac accactctg 
gtggcgagt ccgaacgac gcttcatgg atcgcatca 
atggccgct ctaccgatg gcgttcggt tgcgttttt 
ggtggtttt ctaatggag aatttcggc cgtaaaacc 
acccagatt ttgacaaca atcccctct ttaatcggt 
tggctcctg atcggtttt tccagcgac atttggatg 
attttaact gggcgtttc ctgacgggg ctgtgtggg 
gggctgctg ggcccctca acgggggtg tacatcagt 
gagacgtcc gagccccgg ttcaggggc ttcctcctc 
gcgtccatt tccctggcc atggccctg gggctgttt 
ttcgtccac tttctgggc actttcctc acctggagg 
accacaagt ggcctgtcc ctcatttta ccagtctta 
agtctagtg gtcttgaat ttagtgcca gaatcgcca 
tcatggttg gccaagaag gggcggaat gacgaggcg 
caaaaatca tttttctgg tgccgtggg gaaagtgac 
caggcgagg aaagaacta accgaaatg ttgcaacgt 
tacaaaaac cagacttta gagacggaa aaaattaaa 
ctgatttcg ggcgaattt ctcaagcct ttggccata 
attgtggtt tttattgtg accaatcag tgggccggt 
gttaacgct ttgaccttc tacacggtg accatcatg 
ggcaaaacg ctgggtccg gggctggac gagtatttg 
gccatgctg gtggtggac ctaatcagg gtggctatg 
tcgatcgcc acttgtttc atcatgaag cggattggg 
cgccgccca cttgctcta atcagcggt tttggcact 
tttacctca ttgttcctc ttaagtgcg tatagtttt 
accactcgc ttcgttcgt ttggacttt ccgttggta 
ccaatgggc gcgctcgtg gcttacatc acgttcatt 
accattggc ttcgtgccg ttgccatgg gccatgatg 
ggcgaggtt ttcccccaa acccatcgc aatgtcggg 
tcgagtgtt tcgtcattt atggcgttt gtggcgttt 
ttcagtgtt gttaaaaca agtcctgct atgtttgat 
tgtttgggt accgatggt acgtttatg gtttatggt 
gccgtggcg tttttcggt acaattttc aattggttc 
tttctgccc gaaaccaaa gataagact ttgggcgaa 
attgaagat gagtttaaa tatttattt ttaactgat 
ggtgagacg tgcttggta aattgtgac gaaagggaa 
atgattgat tgtgagagg tttgcgcca agggtgagg 
cagacgttc gtagccttg ggccccatc ctcatcacc 
acctccttg ggcatgacc gagggcttc tccgccatc 
ctcctcccc cagctcaac tccacgtcc ctccaaatc 
gacgaagaa acctcctct tggatcgcc tccatggcc 
gccttgccc atggccttg ggctgcatc ctggggggc 
atcctcatg gagaagatc ggtcggaag gccacccac 
atgctcact tgcctcccc tgcgttatc ggctggctc 
atcctctat ttcgcctcc tcggtggac atgatcctc 
gtcggccgc ttcctcacc ggcttttgc gtgggactc 
ctggggccc ccgacaggg gtctacatg agcgagact 
tccgagccc aaattccgg ggcttcctc ctggcttcc 
atcagtttt gccatcgct ttgggcttg tttttgtca 
cacctgatt gggactttt gtcaattgg caggacacg 
gccttgacg tgttgtagt ttcccggtc atttgtctc 
gtttttatg ggctttgcc cccgaaagc cccacttgg 
ttggcgaaa cgggggcgt ctagaggag gccaaaagg 
gcgtttgtt tggtgcaga ggacaatcg gaggaggcg 
gtgaacgag ttagaggtt ttaatcaac cgacagact 
attttaaat caagaggag acgaaaagt ttttgtgaa 
ataattaaa gacttgaag cgaccggaa tttatcaaa 
ccgttggtc attatcgtg gtttttttc gtcacgtgt 
caatggtca ggtcttaac gctatcact ttttactca 
gtcacgata attcagcag actttgggc ggaaacttt 
gatgagtat ctggcaatg ttgattatt gactcaatt 
agggttttt atgtccgtt ctagcgtgt gtcctttta 
aaaaaatta gggcgccgc cctttggcc ataatcagc 
ggtgttggc actttcgtt tcacttttc attttaagt 
tcgttcact ttcgcggtt aaattttat cccgcaatt 
tcggtctac actttcatt cccttggtc tcattgata 
acgtatgtc agttttatt accattggg ttcgtccca 
ttaccatgg accatgatg ggtgaggtc tttccccta 
gccaatcga ggcataggg agcggaatt tcggcacta 
atggcctac gttgcgttt ttttcggtg gttaaaacc 
acgccagct atgatccaa cattttggg cttgagggc 
acgtttttt atttacgga atgttggca ttggtgggg 
accattatt ttgattttg tttctacca gaaacgaaa 
gacaaggct ttgtaccag atcgaggat aattttaag 
cttgattgt aaataa
    mRNA sequence
    • >TC030899 RA
      tacacaata aaaattatg caaataccc cccggcacg 
cgcaaacac aaatacgct ctttgcaca caagaattc 
aatgtcact tcactttcc aatcgtcac tcattcaag 
aagaaacac cgtgaaagt ccttttcac ttttcattc 
caaccactg ttgtgccaa aatgaagac caaaatcgc 
catcctcca aacgccact aacccgcca aattctcgt 
agccaccgg ccctttgct ctccacaat cggcctcgg 
catgacttc gggcttctc cgccgtgct cttacccca 
actccacaa caccactct ggtggcgag tccgaacga 
cgcttcatg gatcgcatc aatggccgc tctaccgat 
ggcgttcgg ttgcgtttt tggtggttt tctaatgga 
gaatttcgg ccgtaaaac cacccagat tttgacaac 
aatcccctc tttaatcgg ttggctcct gatcggttt 
ttccagcga catttggat gattttaac tgggcgttt 
cctgacggg gctgtgtgg ggggctgct gggcccctc 
aacgggggt gtacatcag tgagacgtc cgagccccg 
gttcagggg cttcctcct cgcgtccat ttccctggc 
catggccct ggggctgtt tttcgtcca ctttctggg 
cactttcct cacctggag gaccacaag tggcctgtc 
cctcatttt accagtctt aagtctagt ggtcttgaa 
tttagtgcc agaatcgcc atcatggtt ggccaagaa 
ggggcggaa tgacgaggc gcaaaaatc atttttctg 
gtgccgtgg ggaaagtga ccaggcgag gaaagaact 
aaccgaaat gttgcaacg ttacaaaaa ccagacttt 
agagacgga aaaaattaa actgatttc gggcgaatt 
tctcaagcc tttggccat aattgtggt ttttattgt 
gaccaatca gtgggccgg tgttaacgc tttgacctt 
ctacacggt gaccatcat gggcaaaac gctgggtcc 
ggggctgga cgagtattt ggccatgct ggtggtgga 
cctaatcag ggtggctat gtcgatcgc cacttgttt 
catcatgaa gcggattgg gcgccgccc acttgctct 
aatcagcgg ttttggcac ttttacctc attgttcct 
cttaagtgc gtatagttt taccactcg cttcgttcg 
tttggactt tccgttggt accaatggg cgcgctcgt 
ggcttacat cacgttcat taccattgg cttcgtgcc 
gttgccatg ggccatgat gggcgaggt tttccccca 
aacccatcg caatgtcgg gtcgagtgt ttcgtcatt 
tatggcgtt tgtggcgtt tttcagtgt tgttaaaac 
aagtcctgc tatgtttga ttgtttggg taccgatgg 
tacgtttat ggtttatgg tgccgtggc gtttttcgg 
tacaatttt caattggtt ctttctgcc cgaaaccaa 
agataagac tttgggcga aattgaaga tgagtttaa 
atatttatt tttaactga tggtgagac gtgcttggt 
aaattgtga cgaaaggga aatgattga ttgtgagag 
gtttgcgcc aagggtgag gcagacgtt cgtagcctt 
gggccccat cctcatcac cacctcctt gggcatgac 
cgagggctt ctccgccat cctcctccc ccagctcaa 
ctccacgtc cctccaaat cgacgaaga aacctcctc 
ttggatcgc ctccatggc cgccttgcc catggcctt 
gggctgcat cctgggggg catcctcat ggagaagat 
cggtcggaa ggccaccca catgctcac ttgcctccc 
ctgcgttat cggctggct catcctcta tttcgcctc 
ctcggtgga catgatcct cgtcggccg cttcctcac 
cggcttttg cgtgggact cctggggcc cccgacagg 
ggtctacat gagcgagac ttccgagcc caaattccg 
gggcttcct cctggcttc catcagttt tgccatcgc 
tttgggctt gtttttgtc acacctgat tgggacttt 
tgtcaattg gcaggacac ggccttgac gtgttgtag 
tttcccggt catttgtct cgtttttat gggctttgc 
ccccgaaag ccccacttg gttggcgaa acgggggcg 
tctagagga ggccaaaag ggcgtttgt ttggtgcag 
aggacaatc ggaggaggc ggtgaacga gttagaggt 
tttaatcaa ccgacagac tattttaaa tcaagagga 
gacgaaaag tttttgtga aataattaa agacttgaa 
gcgaccgga atttatcaa accgttggt cattatcgt 
ggttttttt cgtcacgtg tcaatggtc aggtcttaa 
cgctatcac tttttactc agtcacgat aattcagca 
gactttggg cggaaactt tgatgagta tctggcaat 
gttgattat tgactcaat tagggtttt tatgtccgt 
tctagcgtg tgtcctttt aaaaaaatt agggcgccg 
ccctttggc cataatcag cggtgttgg cactttcgt 
ttcactttt cattttaag ttcgttcac tttcgcggt 
taaatttta tcccgcaat ttcggtcta cactttcat 
tcccttggt ctcattgat aacgtatgt cagttttat 
taccattgg gttcgtccc attaccatg gaccatgat 
gggtgaggt ctttcccct agccaatcg aggcatagg 
gagcggaat ttcggcact aatggccta cgttgcgtt 
tttttcggt ggttaaaac cacgccagc tatgatcca 
acattttgg gcttgaggg cacgttttt tatttacgg 
aatgttggc attggtggg gaccattat tttgatttt 
gtttctacc agaaacgaa agacaaggc tttgtacca 
gatcgagga taattttaa gcttgattg taaataaat 
tattggcga aataaaatt caaaaaaaa atttttttg 
tttgggagg acgtaaagc actacgaaa atgttaata 
aaaattaaa aaaaatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY