| General information | |||
| TC030899
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 1558636..1564082 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC030899 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05827 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC030899 RA
MKTKIAILQ TPLTRQILV ATGPLLSTI GLGMTSGFS AVLLPQLHN TTLVASPND ASWIASMAA LPMAFGCVF GGFLMENFG RKTTQILTT IPSLIGWLL IGFSSDIWM ILTGRFLTG LCGGLLGPS TGVYISETS EPRFRGFLL ASISLAMAL GLFFVHFLG TFLTWRTTS GLSLILPVL SLVVLNLVP ESPSWLAKK GRNDEAQKS FFWCRGESD QARKELTEM LQRYKNQTL ETEKIKLIS GEFLKPLAI IVVFIVTNQ WAGVNALTF YTVTIMGKT LGPGLDEYL AMLVVDLIR VAMSIATCF IMKRIGRRP LALISGFGT FTSLFLLSA YSFTTRFVR LDFPLVPMG ALVAYITFI TIGFVPLPW AMMGEVFPQ THRNVGSSV SSFMAFVAF FSVVKTSPA MFDCLGTDG TFMVYGAVA FFGTIFNWF FLPETKDKT LGEIEDEFK YLFLTDGET CLVNCDERE MIDCERFAP RVRQTFVAL GPILITTSL GMTEGFSAI LLPQLNSTS LQIDEETSS WIASMAALP MALGCILGG ILMEKIGRK ATHMLTCLP CVIGWLILY FASSVDMIL VGRFLTGFC VGLLGPPTG VYMSETSEP KFRGFLLAS ISFAIALGL FLSHLIGTF VNWQDTALT CCSFPVICL VFMGFAPES PTWLAKRGR LEEAKRAFV WCRGQSEEA VNELEVLIN RQTILNQEE TKSFCEIIK DLKRPEFIK PLVIIVVFF VTCQWSGLN AITFYSVTI IQQTLGGNF DEYLAMLII DSIRVFMSV LACVLLKKL GRRPLAIIS GVGTFVSLF ILSSFTFAV KFYPAISVY TFIPLVSLI TYVSFITIG FVPLPWTMM GEVFPLANR GIGSGISAL MAYVAFFSV VKTTPAMIQ HFGLEGTFF IYGMLALVG TIILILFLP ETKDKALYQ IEDNFKLDC K
- >TC030899 RA
atgaagacc aaaatcgcc atcctccaa acgccacta acccgccaa attctcgta gccaccggc cctttgctc tccacaatc ggcctcggc atgacttcg ggcttctcc gccgtgctc ttaccccaa ctccacaac accactctg gtggcgagt ccgaacgac gcttcatgg atcgcatca atggccgct ctaccgatg gcgttcggt tgcgttttt ggtggtttt ctaatggag aatttcggc cgtaaaacc acccagatt ttgacaaca atcccctct ttaatcggt tggctcctg atcggtttt tccagcgac atttggatg attttaact gggcgtttc ctgacgggg ctgtgtggg gggctgctg ggcccctca acgggggtg tacatcagt gagacgtcc gagccccgg ttcaggggc ttcctcctc gcgtccatt tccctggcc atggccctg gggctgttt ttcgtccac tttctgggc actttcctc acctggagg accacaagt ggcctgtcc ctcatttta ccagtctta agtctagtg gtcttgaat ttagtgcca gaatcgcca tcatggttg gccaagaag gggcggaat gacgaggcg caaaaatca tttttctgg tgccgtggg gaaagtgac caggcgagg aaagaacta accgaaatg ttgcaacgt tacaaaaac cagacttta gagacggaa aaaattaaa ctgatttcg ggcgaattt ctcaagcct ttggccata attgtggtt tttattgtg accaatcag tgggccggt gttaacgct ttgaccttc tacacggtg accatcatg ggcaaaacg ctgggtccg gggctggac gagtatttg gccatgctg gtggtggac ctaatcagg gtggctatg tcgatcgcc acttgtttc atcatgaag cggattggg cgccgccca cttgctcta atcagcggt tttggcact tttacctca ttgttcctc ttaagtgcg tatagtttt accactcgc ttcgttcgt ttggacttt ccgttggta ccaatgggc gcgctcgtg gcttacatc acgttcatt accattggc ttcgtgccg ttgccatgg gccatgatg ggcgaggtt ttcccccaa acccatcgc aatgtcggg tcgagtgtt tcgtcattt atggcgttt gtggcgttt ttcagtgtt gttaaaaca agtcctgct atgtttgat tgtttgggt accgatggt acgtttatg gtttatggt gccgtggcg tttttcggt acaattttc aattggttc tttctgccc gaaaccaaa gataagact ttgggcgaa attgaagat gagtttaaa tatttattt ttaactgat ggtgagacg tgcttggta aattgtgac gaaagggaa atgattgat tgtgagagg tttgcgcca agggtgagg cagacgttc gtagccttg ggccccatc ctcatcacc acctccttg ggcatgacc gagggcttc tccgccatc ctcctcccc cagctcaac tccacgtcc ctccaaatc gacgaagaa acctcctct tggatcgcc tccatggcc gccttgccc atggccttg ggctgcatc ctggggggc atcctcatg gagaagatc ggtcggaag gccacccac atgctcact tgcctcccc tgcgttatc ggctggctc atcctctat ttcgcctcc tcggtggac atgatcctc gtcggccgc ttcctcacc ggcttttgc gtgggactc ctggggccc ccgacaggg gtctacatg agcgagact tccgagccc aaattccgg ggcttcctc ctggcttcc atcagtttt gccatcgct ttgggcttg tttttgtca cacctgatt gggactttt gtcaattgg caggacacg gccttgacg tgttgtagt ttcccggtc atttgtctc gtttttatg ggctttgcc cccgaaagc cccacttgg ttggcgaaa cgggggcgt ctagaggag gccaaaagg gcgtttgtt tggtgcaga ggacaatcg gaggaggcg gtgaacgag ttagaggtt ttaatcaac cgacagact attttaaat caagaggag acgaaaagt ttttgtgaa ataattaaa gacttgaag cgaccggaa tttatcaaa ccgttggtc attatcgtg gtttttttc gtcacgtgt caatggtca ggtcttaac gctatcact ttttactca gtcacgata attcagcag actttgggc ggaaacttt gatgagtat ctggcaatg ttgattatt gactcaatt agggttttt atgtccgtt ctagcgtgt gtcctttta aaaaaatta gggcgccgc cctttggcc ataatcagc ggtgttggc actttcgtt tcacttttc attttaagt tcgttcact ttcgcggtt aaattttat cccgcaatt tcggtctac actttcatt cccttggtc tcattgata acgtatgtc agttttatt accattggg ttcgtccca ttaccatgg accatgatg ggtgaggtc tttccccta gccaatcga ggcataggg agcggaatt tcggcacta atggcctac gttgcgttt ttttcggtg gttaaaacc acgccagct atgatccaa cattttggg cttgagggc acgtttttt atttacgga atgttggca ttggtgggg accattatt ttgattttg tttctacca gaaacgaaa gacaaggct ttgtaccag atcgaggat aattttaag cttgattgt aaataa
- >TC030899 RA
tacacaata aaaattatg caaataccc cccggcacg cgcaaacac aaatacgct ctttgcaca caagaattc aatgtcact tcactttcc aatcgtcac tcattcaag aagaaacac cgtgaaagt ccttttcac ttttcattc caaccactg ttgtgccaa aatgaagac caaaatcgc catcctcca aacgccact aacccgcca aattctcgt agccaccgg ccctttgct ctccacaat cggcctcgg catgacttc gggcttctc cgccgtgct cttacccca actccacaa caccactct ggtggcgag tccgaacga cgcttcatg gatcgcatc aatggccgc tctaccgat ggcgttcgg ttgcgtttt tggtggttt tctaatgga gaatttcgg ccgtaaaac cacccagat tttgacaac aatcccctc tttaatcgg ttggctcct gatcggttt ttccagcga catttggat gattttaac tgggcgttt cctgacggg gctgtgtgg ggggctgct gggcccctc aacgggggt gtacatcag tgagacgtc cgagccccg gttcagggg cttcctcct cgcgtccat ttccctggc catggccct ggggctgtt tttcgtcca ctttctggg cactttcct cacctggag gaccacaag tggcctgtc cctcatttt accagtctt aagtctagt ggtcttgaa tttagtgcc agaatcgcc atcatggtt ggccaagaa ggggcggaa tgacgaggc gcaaaaatc atttttctg gtgccgtgg ggaaagtga ccaggcgag gaaagaact aaccgaaat gttgcaacg ttacaaaaa ccagacttt agagacgga aaaaattaa actgatttc gggcgaatt tctcaagcc tttggccat aattgtggt ttttattgt gaccaatca gtgggccgg tgttaacgc tttgacctt ctacacggt gaccatcat gggcaaaac gctgggtcc ggggctgga cgagtattt ggccatgct ggtggtgga cctaatcag ggtggctat gtcgatcgc cacttgttt catcatgaa gcggattgg gcgccgccc acttgctct aatcagcgg ttttggcac ttttacctc attgttcct cttaagtgc gtatagttt taccactcg cttcgttcg tttggactt tccgttggt accaatggg cgcgctcgt ggcttacat cacgttcat taccattgg cttcgtgcc gttgccatg ggccatgat gggcgaggt tttccccca aacccatcg caatgtcgg gtcgagtgt ttcgtcatt tatggcgtt tgtggcgtt tttcagtgt tgttaaaac aagtcctgc tatgtttga ttgtttggg taccgatgg tacgtttat ggtttatgg tgccgtggc gtttttcgg tacaatttt caattggtt ctttctgcc cgaaaccaa agataagac tttgggcga aattgaaga tgagtttaa atatttatt tttaactga tggtgagac gtgcttggt aaattgtga cgaaaggga aatgattga ttgtgagag gtttgcgcc aagggtgag gcagacgtt cgtagcctt gggccccat cctcatcac cacctcctt gggcatgac cgagggctt ctccgccat cctcctccc ccagctcaa ctccacgtc cctccaaat cgacgaaga aacctcctc ttggatcgc ctccatggc cgccttgcc catggcctt gggctgcat cctgggggg catcctcat ggagaagat cggtcggaa ggccaccca catgctcac ttgcctccc ctgcgttat cggctggct catcctcta tttcgcctc ctcggtgga catgatcct cgtcggccg cttcctcac cggcttttg cgtgggact cctggggcc cccgacagg ggtctacat gagcgagac ttccgagcc caaattccg gggcttcct cctggcttc catcagttt tgccatcgc tttgggctt gtttttgtc acacctgat tgggacttt tgtcaattg gcaggacac ggccttgac gtgttgtag tttcccggt catttgtct cgtttttat gggctttgc ccccgaaag ccccacttg gttggcgaa acgggggcg tctagagga ggccaaaag ggcgtttgt ttggtgcag aggacaatc ggaggaggc ggtgaacga gttagaggt tttaatcaa ccgacagac tattttaaa tcaagagga gacgaaaag tttttgtga aataattaa agacttgaa gcgaccgga atttatcaa accgttggt cattatcgt ggttttttt cgtcacgtg tcaatggtc aggtcttaa cgctatcac tttttactc agtcacgat aattcagca gactttggg cggaaactt tgatgagta tctggcaat gttgattat tgactcaat tagggtttt tatgtccgt tctagcgtg tgtcctttt aaaaaaatt agggcgccg ccctttggc cataatcag cggtgttgg cactttcgt ttcactttt cattttaag ttcgttcac tttcgcggt taaatttta tcccgcaat ttcggtcta cactttcat tcccttggt ctcattgat aacgtatgt cagttttat taccattgg gttcgtccc attaccatg gaccatgat gggtgaggt ctttcccct agccaatcg aggcatagg gagcggaat ttcggcact aatggccta cgttgcgtt tttttcggt ggttaaaac cacgccagc tatgatcca acattttgg gcttgaggg cacgttttt tatttacgg aatgttggc attggtggg gaccattat tttgatttt gtttctacc agaaacgaa agacaaggc tttgtacca gatcgagga taattttaa gcttgattg taaataaat tattggcga aataaaatt caaaaaaaa atttttttg tttgggagg acgtaaagc actacgaaa atgttaata aaaattaaa aaaaatc
