Status
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Gene information
General information
TC030899
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015501,TC015500
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1558636..1564082
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030899
    iBeetle screen iB_05827

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030899 RA
      MKTKIAILQ TPLTRQILV ATGPLLSTI GLGMTSGFS 
      AVLLPQLHN TTLVASPND ASWIASMAA LPMAFGCVF 
      GGFLMENFG RKTTQILTT IPSLIGWLL IGFSSDIWM 
      ILTGRFLTG LCGGLLGPS TGVYISETS EPRFRGFLL 
      ASISLAMAL GLFFVHFLG TFLTWRTTS GLSLILPVL 
      SLVVLNLVP ESPSWLAKK GRNDEAQKS FFWCRGESD 
      QARKELTEM LQRYKNQTL ETEKIKLIS GEFLKPLAI 
      IVVFIVTNQ WAGVNALTF YTVTIMGKT LGPGLDEYL 
      AMLVVDLIR VAMSIATCF IMKRIGRRP LALISGFGT 
      FTSLFLLSA YSFTTRFVR LDFPLVPMG ALVAYITFI 
      TIGFVPLPW AMMGEVFPQ THRNVGSSV SSFMAFVAF 
      FSVVKTSPA MFDCLGTDG TFMVYGAVA FFGTIFNWF 
      FLPETKDKT LGEIEDEFK YLFLTDGET CLVNCDERE 
      MIDCERFAP RVRQTFVAL GPILITTSL GMTEGFSAI 
      LLPQLNSTS LQIDEETSS WIASMAALP MALGCILGG 
      ILMEKIGRK ATHMLTCLP CVIGWLILY FASSVDMIL 
      VGRFLTGFC VGLLGPPTG VYMSETSEP KFRGFLLAS 
      ISFAIALGL FLSHLIGTF VNWQDTALT CCSFPVICL 
      VFMGFAPES PTWLAKRGR LEEAKRAFV WCRGQSEEA 
      VNELEVLIN RQTILNQEE TKSFCEIIK DLKRPEFIK 
      PLVIIVVFF VTCQWSGLN AITFYSVTI IQQTLGGNF 
      DEYLAMLII DSIRVFMSV LACVLLKKL GRRPLAIIS 
      GVGTFVSLF ILSSFTFAV KFYPAISVY TFIPLVSLI 
      TYVSFITIG FVPLPWTMM GEVFPLANR GIGSGISAL 
      MAYVAFFSV VKTTPAMIQ HFGLEGTFF IYGMLALVG 
      TIILILFLP ETKDKALYQ IEDNFKLDC K
    CDS sequence
    • >TC030899 RA
      atgaagacc aaaatcgcc atcctccaa acgccacta 
      acccgccaa attctcgta gccaccggc cctttgctc 
      tccacaatc ggcctcggc atgacttcg ggcttctcc 
      gccgtgctc ttaccccaa ctccacaac accactctg 
      gtggcgagt ccgaacgac gcttcatgg atcgcatca 
      atggccgct ctaccgatg gcgttcggt tgcgttttt 
      ggtggtttt ctaatggag aatttcggc cgtaaaacc 
      acccagatt ttgacaaca atcccctct ttaatcggt 
      tggctcctg atcggtttt tccagcgac atttggatg 
      attttaact gggcgtttc ctgacgggg ctgtgtggg 
      gggctgctg ggcccctca acgggggtg tacatcagt 
      gagacgtcc gagccccgg ttcaggggc ttcctcctc 
      gcgtccatt tccctggcc atggccctg gggctgttt 
      ttcgtccac tttctgggc actttcctc acctggagg 
      accacaagt ggcctgtcc ctcatttta ccagtctta 
      agtctagtg gtcttgaat ttagtgcca gaatcgcca 
      tcatggttg gccaagaag gggcggaat gacgaggcg 
      caaaaatca tttttctgg tgccgtggg gaaagtgac 
      caggcgagg aaagaacta accgaaatg ttgcaacgt 
      tacaaaaac cagacttta gagacggaa aaaattaaa 
      ctgatttcg ggcgaattt ctcaagcct ttggccata 
      attgtggtt tttattgtg accaatcag tgggccggt 
      gttaacgct ttgaccttc tacacggtg accatcatg 
      ggcaaaacg ctgggtccg gggctggac gagtatttg 
      gccatgctg gtggtggac ctaatcagg gtggctatg 
      tcgatcgcc acttgtttc atcatgaag cggattggg 
      cgccgccca cttgctcta atcagcggt tttggcact 
      tttacctca ttgttcctc ttaagtgcg tatagtttt 
      accactcgc ttcgttcgt ttggacttt ccgttggta 
      ccaatgggc gcgctcgtg gcttacatc acgttcatt 
      accattggc ttcgtgccg ttgccatgg gccatgatg 
      ggcgaggtt ttcccccaa acccatcgc aatgtcggg 
      tcgagtgtt tcgtcattt atggcgttt gtggcgttt 
      ttcagtgtt gttaaaaca agtcctgct atgtttgat 
      tgtttgggt accgatggt acgtttatg gtttatggt 
      gccgtggcg tttttcggt acaattttc aattggttc 
      tttctgccc gaaaccaaa gataagact ttgggcgaa 
      attgaagat gagtttaaa tatttattt ttaactgat 
      ggtgagacg tgcttggta aattgtgac gaaagggaa 
      atgattgat tgtgagagg tttgcgcca agggtgagg 
      cagacgttc gtagccttg ggccccatc ctcatcacc 
      acctccttg ggcatgacc gagggcttc tccgccatc 
      ctcctcccc cagctcaac tccacgtcc ctccaaatc 
      gacgaagaa acctcctct tggatcgcc tccatggcc 
      gccttgccc atggccttg ggctgcatc ctggggggc 
      atcctcatg gagaagatc ggtcggaag gccacccac 
      atgctcact tgcctcccc tgcgttatc ggctggctc 
      atcctctat ttcgcctcc tcggtggac atgatcctc 
      gtcggccgc ttcctcacc ggcttttgc gtgggactc 
      ctggggccc ccgacaggg gtctacatg agcgagact 
      tccgagccc aaattccgg ggcttcctc ctggcttcc 
      atcagtttt gccatcgct ttgggcttg tttttgtca 
      cacctgatt gggactttt gtcaattgg caggacacg 
      gccttgacg tgttgtagt ttcccggtc atttgtctc 
      gtttttatg ggctttgcc cccgaaagc cccacttgg 
      ttggcgaaa cgggggcgt ctagaggag gccaaaagg 
      gcgtttgtt tggtgcaga ggacaatcg gaggaggcg 
      gtgaacgag ttagaggtt ttaatcaac cgacagact 
      attttaaat caagaggag acgaaaagt ttttgtgaa 
      ataattaaa gacttgaag cgaccggaa tttatcaaa 
      ccgttggtc attatcgtg gtttttttc gtcacgtgt 
      caatggtca ggtcttaac gctatcact ttttactca 
      gtcacgata attcagcag actttgggc ggaaacttt 
      gatgagtat ctggcaatg ttgattatt gactcaatt 
      agggttttt atgtccgtt ctagcgtgt gtcctttta 
      aaaaaatta gggcgccgc cctttggcc ataatcagc 
      ggtgttggc actttcgtt tcacttttc attttaagt 
      tcgttcact ttcgcggtt aaattttat cccgcaatt 
      tcggtctac actttcatt cccttggtc tcattgata 
      acgtatgtc agttttatt accattggg ttcgtccca 
      ttaccatgg accatgatg ggtgaggtc tttccccta 
      gccaatcga ggcataggg agcggaatt tcggcacta 
      atggcctac gttgcgttt ttttcggtg gttaaaacc 
      acgccagct atgatccaa cattttggg cttgagggc 
      acgtttttt atttacgga atgttggca ttggtgggg 
      accattatt ttgattttg tttctacca gaaacgaaa 
      gacaaggct ttgtaccag atcgaggat aattttaag 
      cttgattgt aaataa
    mRNA sequence
    • >TC030899 RA
      tacacaata aaaattatg caaataccc cccggcacg 
      cgcaaacac aaatacgct ctttgcaca caagaattc 
      aatgtcact tcactttcc aatcgtcac tcattcaag 
      aagaaacac cgtgaaagt ccttttcac ttttcattc 
      caaccactg ttgtgccaa aatgaagac caaaatcgc 
      catcctcca aacgccact aacccgcca aattctcgt 
      agccaccgg ccctttgct ctccacaat cggcctcgg 
      catgacttc gggcttctc cgccgtgct cttacccca 
      actccacaa caccactct ggtggcgag tccgaacga 
      cgcttcatg gatcgcatc aatggccgc tctaccgat 
      ggcgttcgg ttgcgtttt tggtggttt tctaatgga 
      gaatttcgg ccgtaaaac cacccagat tttgacaac 
      aatcccctc tttaatcgg ttggctcct gatcggttt 
      ttccagcga catttggat gattttaac tgggcgttt 
      cctgacggg gctgtgtgg ggggctgct gggcccctc 
      aacgggggt gtacatcag tgagacgtc cgagccccg 
      gttcagggg cttcctcct cgcgtccat ttccctggc 
      catggccct ggggctgtt tttcgtcca ctttctggg 
      cactttcct cacctggag gaccacaag tggcctgtc 
      cctcatttt accagtctt aagtctagt ggtcttgaa 
      tttagtgcc agaatcgcc atcatggtt ggccaagaa 
      ggggcggaa tgacgaggc gcaaaaatc atttttctg 
      gtgccgtgg ggaaagtga ccaggcgag gaaagaact 
      aaccgaaat gttgcaacg ttacaaaaa ccagacttt 
      agagacgga aaaaattaa actgatttc gggcgaatt 
      tctcaagcc tttggccat aattgtggt ttttattgt 
      gaccaatca gtgggccgg tgttaacgc tttgacctt 
      ctacacggt gaccatcat gggcaaaac gctgggtcc 
      ggggctgga cgagtattt ggccatgct ggtggtgga 
      cctaatcag ggtggctat gtcgatcgc cacttgttt 
      catcatgaa gcggattgg gcgccgccc acttgctct 
      aatcagcgg ttttggcac ttttacctc attgttcct 
      cttaagtgc gtatagttt taccactcg cttcgttcg 
      tttggactt tccgttggt accaatggg cgcgctcgt 
      ggcttacat cacgttcat taccattgg cttcgtgcc 
      gttgccatg ggccatgat gggcgaggt tttccccca 
      aacccatcg caatgtcgg gtcgagtgt ttcgtcatt 
      tatggcgtt tgtggcgtt tttcagtgt tgttaaaac 
      aagtcctgc tatgtttga ttgtttggg taccgatgg 
      tacgtttat ggtttatgg tgccgtggc gtttttcgg 
      tacaatttt caattggtt ctttctgcc cgaaaccaa 
      agataagac tttgggcga aattgaaga tgagtttaa 
      atatttatt tttaactga tggtgagac gtgcttggt 
      aaattgtga cgaaaggga aatgattga ttgtgagag 
      gtttgcgcc aagggtgag gcagacgtt cgtagcctt 
      gggccccat cctcatcac cacctcctt gggcatgac 
      cgagggctt ctccgccat cctcctccc ccagctcaa 
      ctccacgtc cctccaaat cgacgaaga aacctcctc 
      ttggatcgc ctccatggc cgccttgcc catggcctt 
      gggctgcat cctgggggg catcctcat ggagaagat 
      cggtcggaa ggccaccca catgctcac ttgcctccc 
      ctgcgttat cggctggct catcctcta tttcgcctc 
      ctcggtgga catgatcct cgtcggccg cttcctcac 
      cggcttttg cgtgggact cctggggcc cccgacagg 
      ggtctacat gagcgagac ttccgagcc caaattccg 
      gggcttcct cctggcttc catcagttt tgccatcgc 
      tttgggctt gtttttgtc acacctgat tgggacttt 
      tgtcaattg gcaggacac ggccttgac gtgttgtag 
      tttcccggt catttgtct cgtttttat gggctttgc 
      ccccgaaag ccccacttg gttggcgaa acgggggcg 
      tctagagga ggccaaaag ggcgtttgt ttggtgcag 
      aggacaatc ggaggaggc ggtgaacga gttagaggt 
      tttaatcaa ccgacagac tattttaaa tcaagagga 
      gacgaaaag tttttgtga aataattaa agacttgaa 
      gcgaccgga atttatcaa accgttggt cattatcgt 
      ggttttttt cgtcacgtg tcaatggtc aggtcttaa 
      cgctatcac tttttactc agtcacgat aattcagca 
      gactttggg cggaaactt tgatgagta tctggcaat 
      gttgattat tgactcaat tagggtttt tatgtccgt 
      tctagcgtg tgtcctttt aaaaaaatt agggcgccg 
      ccctttggc cataatcag cggtgttgg cactttcgt 
      ttcactttt cattttaag ttcgttcac tttcgcggt 
      taaatttta tcccgcaat ttcggtcta cactttcat 
      tcccttggt ctcattgat aacgtatgt cagttttat 
      taccattgg gttcgtccc attaccatg gaccatgat 
      gggtgaggt ctttcccct agccaatcg aggcatagg 
      gagcggaat ttcggcact aatggccta cgttgcgtt 
      tttttcggt ggttaaaac cacgccagc tatgatcca 
      acattttgg gcttgaggg cacgttttt tatttacgg 
      aatgttggc attggtggg gaccattat tttgatttt 
      gtttctacc agaaacgaa agacaaggc tttgtacca 
      gatcgagga taattttaa gcttgattg taaataaat 
      tattggcga aataaaatt caaaaaaaa atttttttg 
      tttgggagg acgtaaagc actacgaaa atgttaata 
      aaaattaaa aaaaatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY