Status
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Gene information
General information
TC015477 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9419431..9427123
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015477
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05825

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva segment random decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: Segment shape irregular. Appendages appear rounded.
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015477 RA
      MSGTNVKEF YAHDLVYKF DERKRIVFG VVSESYESS 
      DSDETCALQ KGQILVTWS NPTRKRICR QSKVYLLCR 
      CIAPGDIVR RLEDGKETQ RGYCKKTKQ FATVQIVES 
      DKVIEHVPD SRLCFVKPF QVGDAVCLG DKFGRIQSI 
      DEMIRMQSK CGSIVEVRR SINYNIRDY WFHSINGAY 
      HEAEYPGQT VFCTPMHLR DPKWIKKSK SMMRKMEIG 
      QKFTIQSIE ICLIEVWWY SPNGHTEYA ELKDDEIKR 
      LKVLPYSEN LVMGDRYFL KLKPTDILL KKRDWIKKK 
      SLQSLPHKV PKSDNSTLT NTCAEPFDA DDDWCTEED 
      EESDNKGEI SIYGLHKIK QRKKTYPNL NKFSSGSIV 
      AVQVFCADA RVTVVWQDG TEEKDIPTT ELYYSVSQD 
      DHEFFPGEW VVSNSNENS DKYGVVQRV NFQERTAEI 
      KWFSSVDAN QEPQELATN ESSVYDLKK HVKFVFRPG 
      SVVKAKPTV EDKMGKVID SCPQGYVIV QWLGGKREN 
      CWPQNIELI LESEDFEFP DAFDSDDDL SEDAYSWET 
      ESVESFDGD VTDEINLQN MAARVDFIR NRISYLREA 
      FKHYNITQN FHFVKDLLC IYDNSGYLD KYLGTSFFS 
      TESKHFQAL LLETKKKAK SLGIELRGR MFSRENLSC 
      PTKMKNAEK DNIQKMIKL ESKVNVQIE MCKECSGES 
      CPTTPDSSE PAEKPQENL CVELLVMLK MRMDLIYAE 
      IISRIGGRQ ALTVLTNSS ENPLKPSPS TTTALQNAL 
      LTPETPPKV AKPIELIPK NEAFSILEE APDTHRYFS 
      SKFVPKDLQ KFLVALQKE YKLLKDSLP AGVWVRTYD 
      NRMDLLSVM IRGPAKTPY EDGLFLFDI QLSPDYPKN 
      PPGVHYISY ITEPLNPNL YVEGKVCVS LLGTWMGRG 
      SEMWGPNST LLQLIVSIQ GLILVAQPY FNEAGYERQ 
      THTQQGCEN SRTYNEFVI LKLVQSMTE LLNAAPKVF 
      QNEVLAHFQ AKGEAMCER LMKYCDEEP LVPEFPLLP 
      VSKGFKLSL GSALNGFRQ ALKKALEGR NKEALL
    CDS sequence
    • >TC015477 RA
      atgtccgga acaaacgta aaagaattc tacgctcac 
      gatcttgtc tacaaattc gacgaacgc aagcgaatt 
      gtgtttggg gtagtgagt gagagctat gagagctcg 
      gacagtgac gagacctgt gccctccag aagggccag 
      atcctcgtc acttggagc aaccccacc cggaagcgg 
      atatgcaga cagagcaag gtgtatctg ctgtgccgc 
      tgcatcgcc ccgggggac attgtgcgg cggctggag 
      gacgggaag gagacccag aggggctac tgcaagaag 
      accaagcag ttcgccaca gtccaaatc gtggagagc 
      gacaaagtc atcgagcat gttcccgac agcaggttg 
      tgttttgtt aagccgttc caggtgggg gatgctgtg 
      tgtctgggg gacaagttc gggagaatt cagagcata 
      gacgagatg attaggatg cagtcgaag tgtggttct 
      attgttgaa gttaggaga agtatcaat tataatatt 
      agggattat tggtttcat tccataaat ggggcctat 
      cacgaagcc gagtacccg ggacagact gttttttgt 
      acaccaatg catttacga gacccaaag tggataaaa 
      aaatcaaaa tcgatgatg cgcaagatg gaaatcggc 
      caaaagttc accattcaa agcattgaa atttgcctg 
      atcgaagta tggtggtac tccccgaac ggccacacg 
      gaatacgcc gagctcaag gacgacgaa attaaacga 
      ctgaaagtc ctcccgtac tccgagaat ttggtaatg 
      ggcgatcgc tacttctta aaactaaaa ccgaccgac 
      attttgctt aaaaaacgc gactggatc aagaaaaaa 
      tcgctgcaa tctctccct cacaaagtg cctaaatcg 
      gacaattcc acgctgacg aacacttgc gccgaaccg 
      ttcgacgcc gacgacgac tggtgcaca gaagaagac 
      gaggaatcg gacaataaa ggcgaaatt tccatatac 
      ggcttgcat aaaattaaa cagaggaaa aaaacctat 
      cccaatttg aacaaattc tcctcgggc tcgatcgtc 
      gcggtccag gttttttgt gccgatgcg cgagtcaca 
      gttgtgtgg caggacggc actgaggag aaggatatc 
      ccgacaacg gaactctat tattccgtt tcgcaggac 
      gatcacgaa ttttttcca ggggagtgg gtggtgtcc 
      aactcgaac gaaaactcg gacaagtac ggcgtggtc 
      cagagggtc aatttccaa gaacggacg gctgaaatt 
      aagtggttt tcgagcgtg gacgccaac caggagccg 
      caggaattg gcaacgaac gagtcaagc gtctacgat 
      ttgaaaaag cacgtcaag ttcgtgttc cgtccaggt 
      agtgtagtt aaggccaag ccgaccgtc gaggacaag 
      atgggcaaa gtcatagat agttgtcca caaggctac 
      gtgattgtg cagtggctc ggtggtaag agagaaaat 
      tgttggccg caaaacatc gaattgata ctcgagagt 
      gaagatttc gaatttccg gatgccttt gacagcgat 
      gacgatctt agtgaagat gcgtactcg tgggaaacc 
      gaaagtgtg gagtcgttc gacggcgac gttaccgat 
      gaaattaat ttacaaaat atggccgcc agagttgat 
      tttattagg aacaggatt agttatctg agggaggcc 
      tttaagcac tacaacatc acgcagaat tttcatttt 
      gttaaagat ttgttgtgc atttatgac aattcgggt 
      tatttagat aagtatctc ggcacgtcg ttttttagc 
      acggaaagt aaacatttc caggcgttg ctgcttgaa 
      actaagaaa aaagccaag tctctagga atcgaattg 
      aggggtagg atgttttct cgggaaaat ctttcctgt 
      ccaacaaaa atgaaaaat gccgagaaa gacaatata 
      cagaaaatg atcaagttg gagagtaag gtcaatgtg 
      cagatcgag atgtgcaaa gaatgcagc ggagaatca 
      tgtcctaca acccccgac agctccgag ccggccgaa 
      aaaccccaa gagaacctg tgcgtggaa ctcctcgtg 
      atgctcaaa atgcgcatg gatttaatc tacgcggaa 
      ataatcagt cgcataggc ggccgtcaa gcgttaacc 
      gtcctaaca aactcatcc gaaaacccg ttaaaacca 
      tcgccaagc accaccacc gccttacaa aacgcactt 
      ttaaccccc gaaacgccc ccgaaagtg gccaaaccg 
      atcgaactc atccccaaa aacgaagcc ttctccatt 
      ctggaggaa gcccctgac acccatcgc tatttctcg 
      agcaaattc gtaccaaaa gacttgcaa aaatttcta 
      gtcgcgctc caaaaagag tacaagttg cttaaagac 
      tccctaccg gcaggggtt tgggtccgg acttacgac 
      aaccggatg gacctctta tcagtgatg ataagaggc 
      ccggcgaag actccgtac gaggacggg ttgttcctt 
      ttcgatatt cagttaagt ccggactac cccaagaac 
      cccccgggt gtccattac atcagttac atcacggag 
      cccctgaac ccgaacctg tacgttgag ggcaaagtt 
      tgcgtgtct ttgctgggg acgtggatg gggcgcggg 
      agcgaaatg tgggggcct aacagcacc ctcctgcag 
      ctaattgtg tcaattcag gggcttatc ctcgtagcg 
      cagccctat tttaacgaa gctggctac gaacgacag 
      acgcacacg cagcaaggg tgcgaaaac tcgcgcact 
      tataacgaa tttgttatt ttgaaactt gttcagtcg 
      atgactgag ttgctcaac gccgcacct aaagtattt 
      cagaacgag gtcctcgcg cattttcag gccaaaggc 
      gaggcgatg tgcgaaaga ttgatgaaa tactgcgac 
      gaggaacca cttgtacct gaattcccg ctcttgccc 
      gtcagtaaa gggttcaaa ctgtcgctg ggctcggcc 
      ctaaacggg ttccgacag gcgctgaag aaggccctg 
      gaagggagg aataaagag gcgcttttg taa
    mRNA sequence
    • >TC015477 RA
      ttttgttta taacctgtc atgcgcgtg acgaaaacg 
      tcaaaaacc caatcgcat tggccattt taaacattt 
      ttctcctca gtttcaaaa tcgtagtgt agtttcaac 
      tcgtatcgt aaaaagcaa aaaatgtcc ggaacaaac 
      gtaaaagaa ttctacgct cacgatctt gtctacaaa 
      ttcgacgaa cgcaagcga attgtgttt ggggtagtg 
      agtgagagc tatgagagc tcggacagt gacgagacc 
      tgtgccctc cagaagggc cagatcctc gtcacttgg 
      agcaacccc acccggaag cggatatgc agacagagc 
      aaggtgtat ctgctgtgc cgctgcatc gccccgggg 
      gacattgtg cggcggctg gaggacggg aaggagacc 
      cagaggggc tactgcaag aagaccaag cagttcgcc 
      acagtccaa atcgtggag agcgacaaa gtcatcgag 
      catgttccc gacagcagg ttgtgtttt gttaagccg 
      ttccaggtg ggggatgct gtgtgtctg ggggacaag 
      ttcgggaga attcagagc atagacgag atgattagg 
      atgcagtcg aagtgtggt tctattgtt gaagttagg 
      agaagtatc aattataat attagggat tattggttt 
      cattccata aatggggcc tatcacgaa gccgagtac 
      ccgggacag actgttttt tgtacacca atgcattta 
      cgagaccca aagtggata aaaaaatca aaatcgatg 
      atgcgcaag atggaaatc ggccaaaag ttcaccatt 
      caaagcatt gaaatttgc ctgatcgaa gtatggtgg 
      tactccccg aacggccac acggaatac gccgagctc 
      aaggacgac gaaattaaa cgactgaaa gtcctcccg 
      tactccgag aatttggta atgggcgat cgctacttc 
      ttaaaacta aaaccgacc gacattttg cttaaaaaa 
      cgcgactgg atcaagaaa aaatcgctg caatctctc 
      cctcacaaa gtgcctaaa tcggacaat tccacgctg 
      acgaacact tgcgccgaa ccgttcgac gccgacgac 
      gactggtgc acagaagaa gacgaggaa tcggacaat 
      aaaggcgaa atttccata tacggcttg cataaaatt 
      aaacagagg aaaaaaacc tatcccaat ttgaacaaa 
      ttctcctcg ggctcgatc gtcgcggtc caggttttt 
      tgtgccgat gcgcgagtc acagttgtg tggcaggac 
      ggcactgag gagaaggat atcccgaca acggaactc 
      tattattcc gtttcgcag gacgatcac gaatttttt 
      ccaggggag tgggtggtg tccaactcg aacgaaaac 
      tcggacaag tacggcgtg gtccagagg gtcaatttc 
      caagaacgg acggctgaa attaagtgg ttttcgagc 
      gtggacgcc aaccaggag ccgcaggaa ttggcaacg 
      aacgagtca agcgtctac gatttgaaa aagcacgtc 
      aagttcgtg ttccgtcca ggtagtgta gttaaggcc 
      aagccgacc gtcgaggac aagatgggc aaagtcata 
      gatagttgt ccacaaggc tacgtgatt gtgcagtgg 
      ctcggtggt aagagagaa aattgttgg ccgcaaaac 
      atcgaattg atactcgag agtgaagat ttcgaattt 
      ccggatgcc tttgacagc gatgacgat cttagtgaa 
      gatgcgtac tcgtgggaa accgaaagt gtggagtcg 
      ttcgacggc gacgttacc gatgaaatt aatttacaa 
      aatatggcc gccagagtt gattttatt aggaacagg 
      attagttat ctgagggag gcctttaag cactacaac 
      atcacgcag aattttcat tttgttaaa gatttgttg 
      tgcatttat gacaattcg ggttattta gataagtat 
      ctcggcacg tcgtttttt agcacggaa agtaaacat 
      ttccaggcg ttgctgctt gaaactaag aaaaaagcc 
      aagtctcta ggaatcgaa ttgaggggt aggatgttt 
      tctcgggaa aatctttcc tgtccaaca aaaatgaaa 
      aatgccgag aaagacaat atacagaaa atgatcaag 
      ttggagagt aaggtcaat gtgcagatc gagatgtgc 
      aaagaatgc agcggagaa tcatgtcct acaaccccc 
      gacagctcc gagccggcc gaaaaaccc caagagaac 
      ctgtgcgtg gaactcctc gtgatgctc aaaatgcgc 
      atggattta atctacgcg gaaataatc agtcgcata 
      ggcggccgt caagcgtta accgtccta acaaactca 
      tccgaaaac ccgttaaaa ccatcgcca agcaccacc 
      accgcctta caaaacgca cttttaacc cccgaaacg 
      cccccgaaa gtggccaaa ccgatcgaa ctcatcccc 
      aaaaacgaa gccttctcc attctggag gaagcccct 
      gacacccat cgctatttc tcgagcaaa ttcgtacca 
      aaagacttg caaaaattt ctagtcgcg ctccaaaaa 
      gagtacaag ttgcttaaa gactcccta ccggcaggg 
      gtttgggtc cggacttac gacaaccgg atggacctc 
      ttatcagtg atgataaga ggcccggcg aagactccg 
      tacgaggac gggttgttc cttttcgat attcagtta 
      agtccggac taccccaag aaccccccg ggtgtccat 
      tacatcagt tacatcacg gagcccctg aacccgaac 
      ctgtacgtt gagggcaaa gtttgcgtg tctttgctg 
      gggacgtgg atggggcgc gggagcgaa atgtggggg 
      cctaacagc accctcctg cagctaatt gtgtcaatt 
      caggggctt atcctcgta gcgcagccc tattttaac 
      gaagctggc tacgaacga cagacgcac acgcagcaa 
      gggtgcgaa aactcgcgc acttataac gaatttgtt 
      attttgaaa cttgttcag tcgatgact gagttgctc 
      aacgccgca cctaaagta tttcagaac gaggtcctc 
      gcgcatttt caggccaaa ggcgaggcg atgtgcgaa 
      agattgatg aaatactgc gacgaggaa ccacttgta 
      cctgaattc ccgctcttg cccgtcagt aaagggttc 
      aaactgtcg ctgggctcg gccctaaac gggttccga 
      caggcgctg aagaaggcc ctggaaggg aggaataaa 
      gaggcgctt ttgtaacgt tgttacctt gttatcctg 
      ttctaaacc ttgttaata aaacgtttc gttattttt 
      ggtgatatt tttatgtag gtgtagtat tacagttgt 
      tttgtttcg ttggaattt atttagttc gaatacctc 
      tttgtgatt tttctgtga ttgctgcgc tgtttctta 
      cggtagtat taacgtaat ttaagcaat attgtaatt 
      ttttacgta tcaggaaca tgccaatgg gtcgatagg 
      tcaatcaag aacaaatca ggagaatca ctcaatcta 
      ggattttca agtgcattt ctagacatt aaataaacg 
      ctacgtttc aag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY