Status
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Gene information
General information
TC015472 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9473188..9477932
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015472
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05823

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence -

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Abdomen sometimes constricted.  
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015472 RA
      MNFPTFGVG VPLSAALPV ATQFSAGKI TQSVTTPNG 
      QVVGVLQGG ENGVHYIRP LDANAFATA TTQGQSTGP 
      TQTLITLPI TMPGAKPGD PQQHVQIQV VNPVQTQTF 
      GQGATVLTV AYNPNQEGG IQIVDGQNG IAEGMTVVA 
      ALQPQDIQL IQAEPDKNN KDATPVALI KNELGNKEE 
      ESNEAGTSI TAIPAQYLQ NASVQEYLQ KMQQHNTLP 
      LSLHQFLKF NNPEIKREQ LTGEGDIEA INISTDDQE 
      QLILNEVEG EMETEESAD DPDKGKKKK KYKKKPPKP 
      KKPKPGQVH IATALDGTT LFCCPECHM AYPEKELLE 
      QHLVGHKIE RRFICDICG AGLKRKEHL ERHKLGHNP 
      ERPFVCTVC MKGFKRKEH LNLHFVIHS GEKTEICGE 
      CGKGFYRKD HLRKHARSH ITRRAKEQA ERNQNILIK 
      EPTTQVTIQ VPTSQMQIP VQIQVPQHH IQVTANSEE 
      DGNQVSTVV LPTASEGIM LPQN
    • >TC015472 RB
      MNFPTFGVG VPLSAALPV ATQFSAGKI TQSVTTPNG 
      QVVGVLQGG ENGVHYIRP LDANAFATA TTQGQSTGP 
      TQTLITLPI TMPGAKPGD PQQHVQIQV VNPVQTQVS 
      DTFGQGATV LTVAYNPNQ EGGIQIVDG QNGIAEGMT 
      VVAALQPQD IQLIQAEPD KNNKDATPV ALIKNELGN 
      KEEESNEAG TSITAIPAQ YLQNASVQE YLQKMQQHN 
      TLPLSLHQF LKFNNPEIK REQLTGEGD IEAINISTD 
      DQEQLILNE VEGEMETEE SADDPDKGK KKKKYKKKP 
      PKPKKPKPG QVHIATALD GTTLFCCPE CHMAYPEKE 
      LLEQHLVGH KIERRFICD ICGAGLKRK EHLERHKLG 
      HNPERPFVC TVCMKGFKR KEHLNLHFV IHSGEKTEI 
      CGECGKGFY RKDHLRKHA RSHITRRAK EQAERNQNI 
      LIKEPTTQV TIQVPTSQM QIPVQIQVP QHHIQVTAN 
      SEEDGNQVS TVVLPTASE GIMLPQN
    CDS sequence
    • >TC015472 RA
      atgaacttc cccacgttc ggtgtgggc gtcccccta 
      tcggccgcc cttccagtg gccacccaa ttctccgcc 
      ggaaaaatc acccaaagt gttacaact cccaacgga 
      caagtcgtc ggagtgctg caaggagga gagaatggc 
      gttcactac atcaggcct ttggacgcc aatgccttc 
      gcaaccgcc accacccaa ggccagagc acgggcccc 
      acgcaaacc ctcatcacg ttacctatc accatgccg 
      ggggcgaag cccggcgac ccccagcaa cacgtccaa 
      atccaagtt gttaacccc gtgcagacg cagaccttc 
      ggacaaggg gcgactgtc ttgacggtg gcgtataac 
      ccgaaccag gaggggggc atccagatt gtggatggc 
      cagaacgga attgccgaa gggatgacg gttgtagcc 
      gcccttcag ccgcaagac atccaattg atccaagcc 
      gaacctgac aaaaacaac aaggacgca acccctgtt 
      gctttaatt aaaaacgaa ctgggcaat aaagaggag 
      gagagtaac gaagccgga acttccatc actgcaatc 
      cccgcccaa tatttacaa aacgccagc gtccaggaa 
      tacctacag aagatgcaa caacacaac acgttaccg 
      ctaagcctt caccaattt ctaaaattc aataacccg 
      gaaatcaag cgggaacag ctcaccggt gagggtgac 
      atcgaggcg attaacatc tcaactgac gatcaggag 
      cagctcatt ttgaacgag gtcgaaggc gagatggag 
      acggaagag agcgccgat gaccccgac aagggcaag 
      aagaagaag aagtacaag aagaaacca ccgaagccg 
      aagaaaccc aagccgggg caggtccac atcgctaca 
      gcgctggac ggaacgacg ctgttttgt tgcccggag 
      tgccacatg gcatacccc gagaaggaa ctactcgaa 
      caacactta gtggggcat aaaatcgag cggaggttt 
      atttgcgac atctgcggg gccggtttg aaacgcaaa 
      gaacattta gaacggcac aagttgggg cataacccg 
      gagaggccg ttcgtgtgt acggtctgc atgaagggg 
      ttcaagcgg aaggaacat ttaaatctc cattttgtt 
      attcattcg ggggagaaa acggaaatc tgtggggaa 
      tgcgggaag gggttctac aggaaggac catctgagg 
      aaacacgcc agatctcat ataaccaga cgagcgaaa 
      gagcaagcc gagcggaat cagaatatt ctgatcaaa 
      gaaccgaca acacaagtc acaatacag gtccccacg 
      tctcaaatg cagatccct gtgcagata caggtgccc 
      caacaccac atccaagtt accgccaat tcggaagaa 
      gacggcaac caagtcagc actgtggtc ttgccaact 
      gcgtctgaa gggatcatg ctgccccag aactaa
    • >TC015472 RB
      atgaacttc cccacgttc ggtgtgggc gtcccccta 
      tcggccgcc cttccagtg gccacccaa ttctccgcc 
      ggaaaaatc acccaaagt gttacaact cccaacgga 
      caagtcgtc ggagtgctg caaggagga gagaatggc 
      gttcactac atcaggcct ttggacgcc aatgccttc 
      gcaaccgcc accacccaa ggccagagc acgggcccc 
      acgcaaacc ctcatcacg ttacctatc accatgccg 
      ggggcgaag cccggcgac ccccagcaa cacgtccaa 
      atccaagtt gttaacccc gtgcagacg caggtatct 
      gacaccttc ggacaaggg gcgactgtc ttgacggtg 
      gcgtataac ccgaaccag gaggggggc atccagatt 
      gtggatggc cagaacgga attgccgaa gggatgacg 
      gttgtagcc gcccttcag ccgcaagac atccaattg 
      atccaagcc gaacctgac aaaaacaac aaggacgca 
      acccctgtt gctttaatt aaaaacgaa ctgggcaat 
      aaagaggag gagagtaac gaagccgga acttccatc 
      actgcaatc cccgcccaa tatttacaa aacgccagc 
      gtccaggaa tacctacag aagatgcaa caacacaac 
      acgttaccg ctaagcctt caccaattt ctaaaattc 
      aataacccg gaaatcaag cgggaacag ctcaccggt 
      gagggtgac atcgaggcg attaacatc tcaactgac 
      gatcaggag cagctcatt ttgaacgag gtcgaaggc 
      gagatggag acggaagag agcgccgat gaccccgac 
      aagggcaag aagaagaag aagtacaag aagaaacca 
      ccgaagccg aagaaaccc aagccgggg caggtccac 
      atcgctaca gcgctggac ggaacgacg ctgttttgt 
      tgcccggag tgccacatg gcatacccc gagaaggaa 
      ctactcgaa caacactta gtggggcat aaaatcgag 
      cggaggttt atttgcgac atctgcggg gccggtttg 
      aaacgcaaa gaacattta gaacggcac aagttgggg 
      cataacccg gagaggccg ttcgtgtgt acggtctgc 
      atgaagggg ttcaagcgg aaggaacat ttaaatctc 
      cattttgtt attcattcg ggggagaaa acggaaatc 
      tgtggggaa tgcgggaag gggttctac aggaaggac 
      catctgagg aaacacgcc agatctcat ataaccaga 
      cgagcgaaa gagcaagcc gagcggaat cagaatatt 
      ctgatcaaa gaaccgaca acacaagtc acaatacag 
      gtccccacg tctcaaatg cagatccct gtgcagata 
      caggtgccc caacaccac atccaagtt accgccaat 
      tcggaagaa gacggcaac caagtcagc actgtggtc 
      ttgccaact gcgtctgaa gggatcatg ctgccccag 
      aactaa
    mRNA sequence
    • >TC015472 RA
      ttcaattac atacaattt attctgtta ctaatttat 
      ttgtcgcgt tccatactt ttacaatgg tgggtttta 
      accactggt aactccaac tttgtcact tttctgggg 
      gctgaattg atgtcatta tatcactgt ctcctacag 
      aatattttt ttaaatccc aaaaggatt gcccaagga 
      tatttcaaa caatagcga tcgttgcgt aattaagtc 
      attaaataa cggaaaatc aaaacttaa taattttca 
      tcgttcaaa attaattag cctgcccaa ttatgataa 
      tgactgtga cgttcgttc caaaaacca ctaattggt 
      gcaaaaata aaccgattc cctaatgga aggttgcct 
      tagttgtgt ttgttgtga ttacatcat agcacacga 
      ggtaaccca gatctatgc aaaatagct ttaattgtc 
      tttattgtt acttcttgc ttttggtca aaaacccag 
      cacaggtcg aaagaatcc gccgtgctt gtggcagtt 
      ggtgtgtac aaatccccg tccaaagtc gccttgaac 
      tgaatccat accaatggt atttctctc ccatgaaga 
      gggaaatcg caccatctc gcagactcc atcccggat 
      ggtaaaaag cagaagtaa aagtgttgt gcgaatgta 
      catttctct tagactcct caccatcaa acgcaatag 
      ataaagata acctgcata ctggtcgta ggtgaaagt 
      attctaaag taggtaaat gggttgaca tccccacgg 
      tagatttct ctatcacca tctcagtcc cgcactaca 
      ccagcaggt gcaccagaa gagaacgga acgaggtca 
      catgatcgt tttctacaa cgaataccc cactttcca 
      aattgttct tctgccact cttcgcggt gtctaccac 
      tctgcacct catgtgcac ggttttgat ccactctac 
      cccggcatt tcatcccct cttgggacg tggtggcac 
      tcccaagga cacacttgt caaatttta aactttgtt 
      tggggtttt cggagcggg aaaagttgt gatttgatt 
      ttgtgcaat tttttgtga tactatttt taggtgaac 
      catgaactt ccccacgtt cggtgtggg cgtccccct 
      atcggccgc ccttccagt ggccaccca attctccgc 
      cggaaaaat cacccaaag tgttacaac tcccaacgg 
      acaagtcgt cggagtgct gcaaggagg agagaatgg 
      cgttcacta catcaggcc tttggacgc caatgcctt 
      cgcaaccgc caccaccca aggccagag cacgggccc 
      cacgcaaac cctcatcac gttacctat caccatgcc 
      gggggcgaa gcccggcga cccccagca acacgtcca 
      aatccaagt tgttaaccc cgtgcagac gcagacctt 
      cggacaagg ggcgactgt cttgacggt ggcgtataa 
      cccgaacca ggagggggg catccagat tgtggatgg 
      ccagaacgg aattgccga agggatgac ggttgtagc 
      cgcccttca gccgcaaga catccaatt gatccaagc 
      cgaacctga caaaaacaa caaggacgc aacccctgt 
      tgctttaat taaaaacga actgggcaa taaagagga 
      ggagagtaa cgaagccgg aacttccat cactgcaat 
      ccccgccca atatttaca aaacgccag cgtccagga 
      atacctaca gaagatgca acaacacaa cacgttacc 
      gctaagcct tcaccaatt tctaaaatt caataaccc 
      ggaaatcaa gcgggaaca gctcaccgg tgagggtga 
      catcgaggc gattaacat ctcaactga cgatcagga 
      gcagctcat tttgaacga ggtcgaagg cgagatgga 
      gacggaaga gagcgccga tgaccccga caagggcaa 
      gaagaagaa gaagtacaa gaagaaacc accgaagcc 
      gaagaaacc caagccggg gcaggtcca catcgctac 
      agcgctgga cggaacgac gctgttttg ttgcccgga 
      gtgccacat ggcataccc cgagaagga actactcga 
      acaacactt agtggggca taaaatcga gcggaggtt 
      tatttgcga catctgcgg ggccggttt gaaacgcaa 
      agaacattt agaacggca caagttggg gcataaccc 
      ggagaggcc gttcgtgtg tacggtctg catgaaggg 
      gttcaagcg gaaggaaca tttaaatct ccattttgt 
      tattcattc gggggagaa aacggaaat ctgtgggga 
      atgcgggaa ggggttcta caggaagga ccatctgag 
      gaaacacgc cagatctca tataaccag acgagcgaa 
      agagcaagc cgagcggaa tcagaatat tctgatcaa 
      agaaccgac aacacaagt cacaataca ggtccccac 
      gtctcaaat gcagatccc tgtgcagat acaggtgcc 
      ccaacacca catccaagt taccgccaa ttcggaaga 
      agacggcaa ccaagtcag cactgtggt cttgccaac 
      tgcgtctga agggatcat gctgcccca gaactaaac 
      taggaaata agtactata agtgatctt aatgaacat 
      tgttacact ttttataat gtatatatt atatggaca 
      taatattca tatttattc tctatattt aacgagatg 
      tgtagtttg ttgatgctc cacgacgtg gccatgatc 
      tgaaagaat tgccacgtg aaccaatcc ttgttggtc 
      tgaatcgag tttatttat tttattttt atatgctgt 
      tctttattt ttatttatt cagtattat tgttgtcag 
      actaacact tgatcagtt gcaatactt gtacaattc 
      aatctgttg gaaccaaag gttattcat tctctaaaa 
      gacaccccg cctctctgt acgtggaac cgattatat 
      tttgtacaa aatgattag tttagttgg ttttgggtt 
      taatctaat ttgtaaact ttgagcaat gtatctgta 
      gttcgcttt gtttctggg aaagttggt catatttat 
      aattacgat ttatgttaa ttttagtat aaactctcc 
      tcgcgtcgt ttacttaac aaatttttg ttttaaaaa 
      attcgagag tttactacc ggctaggtc aatgaagac 
      tgcgtttaa aatttgaat aattcaatt tggactctt 
      gattgtgat ccatatgta ggacgtaat ttagtttca 
      caggatgtt gaaacttgg aacgaatgt tgaaattta 
      ctgtatttt tttatacta ctgacattt aattacatt 
      acatgaaaa taaagacgt ttctttttt
    • >TC015472 RB
      ttcaattac atacaattt attctgtta ctaatttat 
      ttgtcgcgt tccatactt ttacaatgg tgggtttta 
      accactggt aactccaac tttgtcact tttctgggg 
      gctgaattg atgtcatta tatcactgt ctcctacag 
      aatattttt ttaaatccc aaaaggatt gcccaagga 
      tatttcaaa caatagcga tcgttgcgt aattaagtc 
      attaaataa cggaaaatc aaaacttaa taattttca 
      tcgttcaaa attaattag cctgcccaa ttatgataa 
      tgactgtga cgttcgttc caaaaacca ctaattggt 
      gcaaaaata aaccgattc cctaatgga aggttgcct 
      tagttgtgt ttgttgtga ttacatcat agcacacga 
      ggtaaccca gatctatgc aaaatagct ttaattgtc 
      tttattgtt acttcttgc ttttggtca aaaacccag 
      cacaggtcg aaagaatcc gccgtgctt gtggcagtt 
      ggtgtgtac aaatccccg tccaaagtc gccttgaac 
      tgaatccat accaatggt atttctctc ccatgaaga 
      gggaaatcg caccatctc gcagactcc atcccggat 
      ggtaaaaag cagaagtaa aagtgttgt gcgaatgta 
      catttctct tagactcct caccatcaa acgcaatag 
      ataaagata acctgcata ctggtcgta ggtgaaagt 
      attctaaag taggtaaat gggttgaca tccccacgg 
      tagatttct ctatcacca tctcagtcc cgcactaca 
      ccagcaggt gcaccagaa gagaacgga acgaggtca 
      catgatcgt tttctacaa cgaataccc cactttcca 
      aattgttct tctgccact cttcgcggt gtctaccac 
      tctgcacct catgtgcac ggttttgat ccactctac 
      cccggcatt tcatcccct cttgggacg tggtggcac 
      tcccaagga cacacttgt caaatttta aactttgtt 
      tggggtttt cggagcggg aaaagttgt gatttgatt 
      ttgtgcaat tttttgtga tactatttt taggtgaac 
      catgaactt ccccacgtt cggtgtggg cgtccccct 
      atcggccgc ccttccagt ggccaccca attctccgc 
      cggaaaaat cacccaaag tgttacaac tcccaacgg 
      acaagtcgt cggagtgct gcaaggagg agagaatgg 
      cgttcacta catcaggcc tttggacgc caatgcctt 
      cgcaaccgc caccaccca aggccagag cacgggccc 
      cacgcaaac cctcatcac gttacctat caccatgcc 
      gggggcgaa gcccggcga cccccagca acacgtcca 
      aatccaagt tgttaaccc cgtgcagac gcaggtatc 
      tgacacctt cggacaagg ggcgactgt cttgacggt 
      ggcgtataa cccgaacca ggagggggg catccagat 
      tgtggatgg ccagaacgg aattgccga agggatgac 
      ggttgtagc cgcccttca gccgcaaga catccaatt 
      gatccaagc cgaacctga caaaaacaa caaggacgc 
      aacccctgt tgctttaat taaaaacga actgggcaa 
      taaagagga ggagagtaa cgaagccgg aacttccat 
      cactgcaat ccccgccca atatttaca aaacgccag 
      cgtccagga atacctaca gaagatgca acaacacaa 
      cacgttacc gctaagcct tcaccaatt tctaaaatt 
      caataaccc ggaaatcaa gcgggaaca gctcaccgg 
      tgagggtga catcgaggc gattaacat ctcaactga 
      cgatcagga gcagctcat tttgaacga ggtcgaagg 
      cgagatgga gacggaaga gagcgccga tgaccccga 
      caagggcaa gaagaagaa gaagtacaa gaagaaacc 
      accgaagcc gaagaaacc caagccggg gcaggtcca 
      catcgctac agcgctgga cggaacgac gctgttttg 
      ttgcccgga gtgccacat ggcataccc cgagaagga 
      actactcga acaacactt agtggggca taaaatcga 
      gcggaggtt tatttgcga catctgcgg ggccggttt 
      gaaacgcaa agaacattt agaacggca caagttggg 
      gcataaccc ggagaggcc gttcgtgtg tacggtctg 
      catgaaggg gttcaagcg gaaggaaca tttaaatct 
      ccattttgt tattcattc gggggagaa aacggaaat 
      ctgtgggga atgcgggaa ggggttcta caggaagga 
      ccatctgag gaaacacgc cagatctca tataaccag 
      acgagcgaa agagcaagc cgagcggaa tcagaatat 
      tctgatcaa agaaccgac aacacaagt cacaataca 
      ggtccccac gtctcaaat gcagatccc tgtgcagat 
      acaggtgcc ccaacacca catccaagt taccgccaa 
      ttcggaaga agacggcaa ccaagtcag cactgtggt 
      cttgccaac tgcgtctga agggatcat gctgcccca 
      gaactaaac taggaaata agtactata agtgatctt 
      aatgaacat tgttacact ttttataat gtatatatt 
      atatggaca taatattca tatttattc tctatattt 
      aacgagatg tgtagtttg ttgatgctc cacgacgtg 
      gccatgatc tgaaagaat tgccacgtg aaccaatcc 
      ttgttggtc tgaatcgag tttatttat tttattttt 
      atatgctgt tctttattt ttatttatt cagtattat 
      tgttgtcag actaacact tgatcagtt gcaatactt 
      gtacaattc aatctgttg gaaccaaag gttattcat 
      tctctaaaa gacaccccg cctctctgt acgtggaac 
      cgattatat tttgtacaa aatgattag tttagttgg 
      ttttgggtt taatctaat ttgtaaact ttgagcaat 
      gtatctgta gttcgcttt gtttctggg aaagttggt 
      catatttat aattacgat ttatgttaa ttttagtat 
      aaactctcc tcgcgtcgt ttacttaac aaatttttg 
      ttttaaaaa attcgagag tttactacc ggctaggtc 
      aatgaagac tgcgtttaa aatttgaat aattcaatt 
      tggactctt gattgtgat ccatatgta ggacgtaat 
      ttagtttca caggatgtt gaaacttgg aacgaatgt 
      tgaaattta ctgtatttt tttatacta ctgacattt 
      aattacatt acatgaaaa taaagacgt ttctttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY