Status
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Gene information
General information
TC015464 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9561883..9573503
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015464
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05822

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015464 RA
      MNKTIFLFL LAYATATKL LEMEYKEGT KVDPKYSLP 
      QVNLSASDG NHTVVPASL ARVLNFFNS EILAANWET 
      TKKLVSLSC RKDVELYLE GLRKTENWA LKMDDASGR 
      YSTGWFWGN QYWTGSQSL CENIVPRSK SIVVNPENR 
      TRTSREAEV PRRPSSPSQ SVGYVTGPL KFSSLPPFP 
      ISFFMLRID VNSSFTTEE RILHIGLCM PYVCTDSDI 
      KVIMEETSK ASTKVTVKV EAVRSHHNR FNIWQDRTF 
      IILCGVTSF VVVCLIIGT SYDFYLEHE EKRKKLMSI 
      SQTHLKLDM SMPDTKTKN GVYTVNNNN EETEAGLKT 
      EAKPRTLRD IVKLIVKET VLAFSLRAN IRTICDQSV 
      SSDTIPVIH GLKSISMAW VILGHTCII AFKYSDNME 
      YRKVVQREF FFQTISNGA FSVDTFFFT SGLLVSFLY 
      FRTNAKGKL APITIGHNG FISGITHFF GLILYRFAR 
      LTAPYLFTL GVVEVIMKW FNYNSIFEP PTMDHVNCP 
      NYWWRNVLY INTLFPVEE MCMLWSWYL SDDTQFYIV 
      GAVMLILAA SHFKSGACL LLIFMVSSW ITTGYIAYS 
      NSHMPGSDD PLALFDKIY DKPWTRLGP YLIGMCVGW 
      FLFKKNCQL TMSKLAVVS GWTAAIACL LSLVYGLYE 
      ANLSPLAGA AYSALSHSA WALGLAWIV VACSTGYGG 
      FANSVLSST ILYPFSRIT YCAYLLHPV VIRVMTMSM 
      DSPLHLGSL VMIIIFLGQ VVASYVLSF FVSLAFEAP 
      VVSMLRIMT KVVGTKK
    CDS sequence
    • >TC015464 RA
      atgaacaaa accatcttc ctattcctg ctggcctac 
      gccactgcg accaaactc ctcgaaatg gagtacaag 
      gaagggacc aaagtggac ccgaaatac tccctgccc 
      caagtgaac ctgagtgcg agtgacggt aatcataca 
      gtggtgccg gcgagttta gccagagtt ttgaatttt 
      tttaattcg gaaattttg gcggccaat tgggaaacg 
      acgaaaaag ctcgtctcg ttaagctgc cggaaggat 
      gtggagctg tatctcgaa ggactcagg aagaccgag 
      aactgggcg ttgaaaatg gacgatgcc agtggccgg 
      tactctaca gggtggttc tggggcaac caatactgg 
      accggctcg caaagttta tgcgaaaat attgtccca 
      agaagcaaa tccatcgta gtgaatccg gagaatcgg 
      acacgaact tcgagagaa gctgaagta cctcgaagg 
      ccttcaagc ccaagtcag tcagtcggt tacgtcacc 
      ggtcctttg aaattctcc tctctgcca cccttcccc 
      atatcgttc tttatgctg cgaattgat gttaattca 
      agcttcacc acggaagaa cgcatcctc cacattggt 
      ctctgcatg ccttacgtt tgcactgac tctgatatt 
      aaagtaatt atggaagaa acatccaaa gcttcaacc 
      aaagtcacg gtgaaagtc gaagctgtg aggtcgcac 
      cacaaccga ttcaacatt tggcaagac cgaacgttt 
      atcatttta tgtggcgta acgtcgttc gttgtcgtt 
      tgtttgatc attggaact tcgtacgat ttctatttg 
      gaacacgag gaaaaacgc aaaaagctc atgtctatc 
      agtcagacg cacctaaag cttgatatg agtatgccg 
      gacactaaa acgaaaaac ggtgtttat actgtcaat 
      aacaacaac gaggagact gaagctggg cttaaaact 
      gaagcgaaa ccgaggact ttgagggat attgttaaa 
      ctaatagta aaggaaact gtacttgca ttttcacta 
      agggcgaat attaggacg atttgtgac cagtctgtt 
      agcagtgac acgattccg gtcatccat gggcttaaa 
      tccatcagc atggcttgg gttattttg ggacacact 
      tgcattatt gcattcaaa tattcagac aacatggag 
      tacaggaaa gtggtccaa agagaattt ttcttccaa 
      accatctca aacggagcg ttcagcgta gatacattt 
      ttctttact agcggtctt ctagtctca tttttgtac 
      ttcagaaca aacgccaag ggaaaacta gctcccatt 
      acaattggt cataatggt ttcatttcg ggaataacc 
      cactttttt ggcctcatc ttgtacaga tttgcacga 
      ttgactgcg ccttatctg ttcactttg ggcgtagtt 
      gaagtaatc atgaaatgg ttcaactac aactcaatt 
      ttcgaacct ccgaccatg gaccatgtc aactgtccc 
      aactactgg tggaggaac gttctgtac attaacaca 
      ttattccca gttgaagaa atgtgtatg ttatggagc 
      tggtattta tcagacgac acccaattt tacatcgtt 
      ggtgcagtg atgttgatt ctagcagcc agtcatttc 
      aagagtggc gcttgtctc ttgttgatt ttcatggtt 
      agttcgtgg attacgacc ggttatata gcttacagc 
      aacagtcat atgccaggc tcggatgac cctcttgcc 
      ctttttgac aaaatttac gacaaacct tggacccga 
      ctaggccct tatttgatc ggcatgtgc gttgggtgg 
      ttccttttc aagaaaaac tgccaactt acaatgtca 
      aagttggcc gtggtgtcc ggctggacg gccgcaata 
      gcgtgtctt ttatccctt gtttacggt ttatacgag 
      gccaattta tcgccgcta gctggcgct gcttacagt 
      gcattaagt cactcggct tgggctctc ggtttagca 
      tggattgtt gtggcatgt tctactgga tatggaggt 
      tttgccaat tcggtttta tcatcgacc attttgtat 
      cccttcagt cggattaca tactgtgcc tatttgcta 
      catccagtg gttattcga gtgatgaca atgagtatg 
      gatagtccc ctccatttg gggagttta gtcatgatt 
      ataattttt ctgggccaa gttgtggca tcgtatgtt 
      ctttcgttt ttcgtttct ttggcgttt gaagcacct 
      gttgtgtcc atgttgagg ataatgacg aaggtggta 
      ggcaccaaa aaatga
    mRNA sequence
    • >TC015464 RA
      ttcagttta cataagttt tccggtttt ataccagaa 
      gtttggact acaaataat gcaataatt gaattataa 
      cagtacact attaactgt gtatttcaa aatcgtatt 
      ttttgcaaa tcgtctcga agtttcgat cccaagcgg 
      gtggggtgt cccatggac aacaattgt taaacactc 
      aaagagaga gaaagttag cgggactaa acctccgcg 
      agcacatgg caatgtgca ttcaacacc atattcttg 
      aacttgtcc gaagcgcgc gttcgtcat aaaataata 
      caagaaaat gaacaaaac catcttcct attcctgct 
      ggcctacgc cactgcgac caaactcct cgaaatgga 
      gtacaagga agggaccaa agtggaccc gaaatactc 
      cctgcccca agtgaacct gagtgcgag tgacggtaa 
      tcatacagt ggtgccggc gagtttagc cagagtttt 
      gaatttttt taattcgga aattttggc ggccaattg 
      ggaaacgac gaaaaagct cgtctcgtt aagctgccg 
      gaaggatgt ggagctgta tctcgaagg actcaggaa 
      gaccgagaa ctgggcgtt gaaaatgga cgatgccag 
      tggccggta ctctacagg gtggttctg gggcaacca 
      atactggac cggctcgca aagtttatg cgaaaatat 
      tgtcccaag aagcaaatc catcgtagt gaatccgga 
      gaatcggac acgaacttc gagagaagc tgaagtacc 
      tcgaaggcc ttcaagccc aagtcagtc agtcggtta 
      cgtcaccgg tcctttgaa attctcctc tctgccacc 
      cttccccat atcgttctt tatgctgcg aattgatgt 
      taattcaag cttcaccac ggaagaacg catcctcca 
      cattggtct ctgcatgcc ttacgtttg cactgactc 
      tgatattaa agtaattat ggaagaaac atccaaagc 
      ttcaaccaa agtcacggt gaaagtcga agctgtgag 
      gtcgcacca caaccgatt caacatttg gcaagaccg 
      aacgtttat cattttatg tggcgtaac gtcgttcgt 
      tgtcgtttg tttgatcat tggaacttc gtacgattt 
      ctatttgga acacgagga aaaacgcaa aaagctcat 
      gtctatcag tcagacgca cctaaagct tgatatgag 
      tatgccgga cactaaaac gaaaaacgg tgtttatac 
      tgtcaataa caacaacga ggagactga agctgggct 
      taaaactga agcgaaacc gaggacttt gagggatat 
      tgttaaact aatagtaaa ggaaactgt acttgcatt 
      ttcactaag ggcgaatat taggacgat ttgtgacca 
      gtctgttag cagtgacac gattccggt catccatgg 
      gcttaaatc catcagcat ggcttgggt tattttggg 
      acacacttg cattattgc attcaaata ttcagacaa 
      catggagta caggaaagt ggtccaaag agaattttt 
      cttccaaac catctcaaa cggagcgtt cagcgtaga 
      tacattttt ctttactag cggtcttct agtctcatt 
      tttgtactt cagaacaaa cgccaaggg aaaactagc 
      tcccattac aattggtca taatggttt catttcggg 
      aataaccca cttttttgg cctcatctt gtacagatt 
      tgcacgatt gactgcgcc ttatctgtt cactttggg 
      cgtagttga agtaatcat gaaatggtt caactacaa 
      ctcaatttt cgaacctcc gaccatgga ccatgtcaa 
      ctgtcccaa ctactggtg gaggaacgt tctgtacat 
      taacacatt attcccagt tgaagaaat gtgtatgtt 
      atggagctg gtatttatc agacgacac ccaatttta 
      catcgttgg tgcagtgat gttgattct agcagccag 
      tcatttcaa gagtggcgc ttgtctctt gttgatttt 
      catggttag ttcgtggat tacgaccgg ttatatagc 
      ttacagcaa cagtcatat gccaggctc ggatgaccc 
      tcttgccct ttttgacaa aatttacga caaaccttg 
      gacccgact aggccctta tttgatcgg catgtgcgt 
      tgggtggtt ccttttcaa gaaaaactg ccaacttac 
      aatgtcaaa gttggccgt ggtgtccgg ctggacggc 
      cgcaatagc gtgtctttt atcccttgt ttacggttt 
      atacgaggc caatttatc gccgctagc tggcgctgc 
      ttacagtgc attaagtca ctcggcttg ggctctcgg 
      tttagcatg gattgttgt ggcatgttc tactggata 
      tggaggttt tgccaattc ggttttatc atcgaccat 
      tttgtatcc cttcagtcg gattacata ctgtgccta 
      tttgctaca tccagtggt tattcgagt gatgacaat 
      gagtatgga tagtcccct ccatttggg gagtttagt 
      catgattat aatttttct gggccaagt tgtggcatc 
      gtatgttct ttcgttttt cgtttcttt ggcgtttga 
      agcacctgt tgtgtccat gttgaggat aatgacgaa 
      ggtggtagg caccaaaaa atgaacagt gactgtgaa 
      tataagttt gtttttaaa ttattattt ttatgtaaa 
      tagttgtgt acctttcaa gactttgta gtttgtaaa 
      ctagtttta ggttgtcag gcttttaca cagccttag 
      aataattgt atttgtgtg ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY