| General information | |||
| TC033340
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 9630888..9636133 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033340 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05820 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC033340 RA
MLLVVLLVL GTTLVGANL PQVTLPDLG QLEGTHGTS LKGRTFHKF EGVPYARPP VGKYRFREP VPPKPWQGV WQAKTLYKC MQNFQYTPP GGDFVIGDE DCLYLNIYS PNIDPKANL DVIFFIHGG CFMFNYAGF QGPEYLLDR DVVYVSVNY RLGPLGFLC TEDEVAPGN NGLKDQIFA LQFVKKYIR YFGGNPDSV TISGMSAGG ASVHFHYLS PKSRGLYHR GISQSGTML NQWVLTEKP LEKAKKLAA LLGCSTESS DKMVECLKW RPGRQIVEA VKEFQPWLY NPYTPFGPV VDSWAQDPV LPQHPYILI KEGKFTDLP WLASYTSSE GLYPASDFY IDEQYLEDI DRRWNEIVP FILDYNNTV ESQLRDEVS QKIRKYYLG DKKVSRSTF MDFVKITTD RVFVDGVYN TIRMQAPLM KSPLYSYFF EYRGAHSRS ESRTGTNKN FGAAHGDDT AYVFKTSVD TASTENDQK VVELLVDIF TSFAKNGVP KVPLEWPPV SRSTDSIIQ LKIAGPNDL SLVESKFKN GDFWRSLPL MENEKLFEN RKDELEIPN HKCGLPIHG ETLSYRIHS GLFVGFRRR LWKWKTVSN SIPESRTVL KSTAQIYIE EQRHFHRHP CIIHPYSQA RFLWEMFMI PVFVLLFIT IPVVLSNSK VNSGIVVVF YIKLGLDAI SFLDIMLNF CTGYYDVKQ QKIVLSAKE IAKSYILSY FIFDVISST PCHMILHWL RRDEDNVWE MLTLLKLVR LPTLVNYLR KLGERWKMT SAMKFICFF LWVSVLLIW LTSLIFLIM RMWHSRSMV KARIKNFPM LFTFFRIVR CMMLVGVTE KMNTSTVVM VFQIGGFAM GAILQIVIL SKLMQLFQK RASSKSKYN TLIEEIGEY MKFKELPTQ LKDRILKYV DFKFQKHIF REEDVLNNL SSVLKQDIL IHRCQKMVE KVEFFKDLP THVLLRIVT KLRSEIFLP NDVIVLAGY AGHAMYFIY MGTVAVFTG HGKEICHLE DGGHFGEIA LVINEPRVA TVIAVKECE LFKLSRRDF QEAIEPFPE LNKKIRQLA MLRLESTLQ ILSENKEEI RIRHESMVE TEDDEFIDT ISI
- >TC033340 RA
atgttactg gtcgtttta ctggtctta ggtaccacc cttgtgggt gcaaatttg ccccaagtc acactaccc gatttaggg caacttgaa ggtacccat ggtactagc ctcaaaggc cgtactttt cacaagttc gagggagta ccatatgca agaccacct gtcggcaag taccgtttc cgggaaccg gtaccacca aaaccctgg caaggggta tggcaagcc aaaactttg tacaaatgt atgcaaaac ttccagtac acgccacca gggggcgac ttcgtcatt ggcgatgag gattgtctt tatttgaac atttatagc cccaatatt gaccctaaa gccaacttg gacgtgatt tttttcata catgggggc tgttttatg tttaattat gccggtttc caaggccct gagtactta ctggaccgg gacgtagtt tacgtcagt gtgaactat agattaggc cctttgggc tttttgtgt actgaagac gaagtggca cccggaaac aacgggtta aaggaccaa attttcgcc ctccaattt gtcaaaaag tacattcgg tactttggt ggtaaccct gattcggtc acaatttcg ggaatgtca gctgggggg gcaagtgta catttccac tacttgtcg cccaaatcg cgcgggttg taccaccgg gggatttcc caaagtggt accatgctc aaccagtgg gtactgacc gaaaaaccg ctcgaaaaa gccaaaaaa ttggcagct ttgctgggg tgtagtacc gaaagtagc gacaagatg gtggagtgt ttgaaatgg cggcctggg cgacagatt gtcgaagca gtgaaggag ttccagcct tggttgtac aacccctac acgccgttt ggcccagtt gtggactcg tgggcccaa gacccggta ctaccccaa catccttat attttaatc aaggagggg aaattcacg gatttgcct tggttggcg tcttatacg agttcagaa gggttgtac ccagcttca gatttttac atcgatgag cagtacttg gaagatatt gacaggcgt tggaacgag atagtaccc tttattttg gattataac aacactgtt gagtcccag ttgcgagac gaagtcagt caaaaaata cgaaagtac taccttggt gataagaaa gtttcaagg agtactttc atggacttt gtcaaaata accacagat cgggttttt gtcgatggg gtttataac acaatccga atgcaagca ccactgatg aagtcaccc ctttattcg tactttttc gagtaccgg ggggcccac agtcgctcc gaaagtcgc actggtacc aacaaaaat ttcggagct gcacatggt gacgataca gcttatgtt ttcaaaact agtgttgac acagcaagt accgaaaat gaccaaaag gtggtcgaa ctcctagtt gatattttc acctctttt gccaaaaat ggagtacct aaagtacca cttgaatgg cccccagtt tcgagaagt accgactcg ataattcag ttaaaaatc gcaggtccg aacgactta tccctagtc gagtccaag tttaaaaat ggcgatttt tggcgaagt ttgccgttg atggagaac gaaaaactt tttgaaaac agaaaagac gaactggaa atccccaac cacaaatgt gggctccca attcacggg gaaaccctg tcgtaccgc atccacagc ggcttgttc gtgggcttc cgccggcgg ctgtggaaa tggaaaacc gtcagcaac tcaattccg gaaagcagg accgtactc aagtccacg gcccaaatt tacatcgag gagcagcgc cactttcat cgccacccc tgtataatc catccctac agtcaggcg cgcttcctg tgggaaatg ttcatgatt cccgttttt gttctcctg ttcatcaca ataccggtc gtgctctcc aactccaaa gtcaatagt ggaatagtg gtggtcttc tacatcaag ctgggcctg gatgccatt tccttcctg gacataatg ctcaatttc tgcaccggg tactacgat gtgaagcag cagaaaatc gtcttgtcg gcgaaggaa attgcaaaa agttacatc ctctcctat tttatcttt gacgttatt tcgtctaca ccgtgtcat atgatatta cattggctg agacgggat gaggataac gtctgggaa atgctaact ttgctgaaa cttgtgcgg ttgccgaca cttgtcaat tatttgcgc aaattggga gagcggtgg aaaatgacg tccgccatg aagttcatt tgcttcttc ctgtgggtg tcagttttg ttaatttgg ctcacaagt ctgatcttt ctgatcatg agaatgtgg cactctcgt tcaatggtc aaggcaaga atcaaaaat tttcccatg cttttcacc ttctttcga atcgtgaga tgcatgatg ttagtcggc gtaaccgag aaaatgaac accagtact gtggtgatg gttttccaa atcggcggt ttcgcaatg ggcgctatt ttacaaatt gtgatatta tcgaaactg atgcagttg ttccaaaag cgggccagt tccaagagc aaatacaac actctcatt gaagaaatc ggagaatat atgaaattc aaggaactg ccaacgcag ctgaaagac cgcattctg aaatacgtg gactttaag ttccaaaag cacattttc cgggaggag gacgtcctg aataattta tcctcggtc ctgaagcag gacattttg atacacagg tgccagaaa atggtggag aaagtggaa tttttcaag gacttgccc actcatgtc ttacttcga attgtgaca aaactacga tctgagatt tttttgccc aatgatgtg atcgttctg gccggatat gcaggacat gcgatgtat tttatctac atggggaca gtggccgtg tttacggga cacgggaaa gagatttgt cacttggag gacggtggg catttcggt gaaattgcc ctggtgatt aacgagcca cgtgttgcc actgtcatt gctgtgaaa gaatgcgag ttgttcaag ttgagtcgg agggatttt caagaggcg attgagccg tttcctgaa cttaataaa aaaatacgg cagttggca atgctcagg cttgagagc acgctgcag attttgtcg gagaataaa gaggagata agaatacga cacgagtcg atggtggag accgaggat gatgagttt attgatact atttcgatt tag
- >TC033340 RA
ctacaagcg caaagtcat ggtaccact tatcagctg tcttataaa attcgtcgt cttgcgtaa ccattagta ttagtgaag tcatgttac tggtcgttt tactggtct taggtacca cccttgtgg gtgcaaatt tgccccaag tcacactac ccgatttag ggcaacttg aaggtaccc atggtacta gcctcaaag gccgtactt ttcacaagt tcgagggag taccatatg caagaccac ctgtcggca agtaccgtt tccgggaac cggtaccac caaaaccct ggcaagggg tatggcaag ccaaaactt tgtacaaat gtatgcaaa acttccagt acacgccac cagggggcg acttcgtca ttggcgatg aggattgtc tttatttga acatttata gccccaata ttgacccta aagccaact tggacgtga tttttttca tacatgggg gctgtttta tgtttaatt atgccggtt tccaaggcc ctgagtact tactggacc gggacgtag tttacgtca gtgtgaact atagattag gccctttgg gctttttgt gtactgaag acgaagtgg cacccggaa acaacgggt taaaggacc aaattttcg ccctccaat ttgtcaaaa agtacattc ggtactttg gtggtaacc ctgattcgg tcacaattt cgggaatgt cagctgggg gggcaagtg tacatttcc actacttgt cgcccaaat cgcgcgggt tgtaccacc gggggattt cccaaagtg gtaccatgc tcaaccagt gggtactga ccgaaaaac cgctcgaaa aagccaaaa aattggcag ctttgctgg ggtgtagta ccgaaagta gcgacaaga tggtggagt gtttgaaat ggcggcctg ggcgacaga ttgtcgaag cagtgaagg agttccagc cttggttgt acaacccct acacgccgt ttggcccag ttgtggact cgtgggccc aagacccgg tactacccc aacatcctt atattttaa tcaaggagg ggaaattca cggatttgc cttggttgg cgtcttata cgagttcag aagggttgt acccagctt cagattttt acatcgatg agcagtact tggaagata ttgacaggc gttggaacg agatagtac cctttattt tggattata acaacactg ttgagtccc agttgcgag acgaagtca gtcaaaaaa tacgaaagt actaccttg gtgataaga aagtttcaa ggagtactt tcatggact ttgtcaaaa taaccacag atcgggttt ttgtcgatg gggtttata acacaatcc gaatgcaag caccactga tgaagtcac ccctttatt cgtactttt tcgagtacc ggggggccc acagtcgct ccgaaagtc gcactggta ccaacaaaa atttcggag ctgcacatg gtgacgata cagcttatg ttttcaaaa ctagtgttg acacagcaa gtaccgaaa atgaccaaa aggtggtcg aactcctag ttgatattt tcacctctt ttgccaaaa atggagtac ctaaagtac cacttgaat ggcccccag tttcgagaa gtaccgact cgataattc agttaaaaa tcgcaggtc cgaacgact tatccctag tcgagtcca agtttaaaa atggcgatt tttggcgaa gtttgccgt tgatggaga acgaaaaac tttttgaaa acagaaaag acgaactgg aaatcccca accacaaat gtgggctcc caattcacg gggaaaccc tgtcgtacc gcatccaca gcggcttgt tcgtgggct tccgccggc ggctgtgga aatggaaaa ccgtcagca actcaattc cggaaagca ggaccgtac tcaagtcca cggcccaaa tttacatcg aggagcagc gccactttc atcgccacc cctgtataa tccatccct acagtcagg cgcgcttcc tgtgggaaa tgttcatga ttcccgttt ttgttctcc tgttcatca caataccgg tcgtgctct ccaactcca aagtcaata gtggaatag tggtggtct tctacatca agctgggcc tggatgcca tttccttcc tggacataa tgctcaatt tctgcaccg ggtactacg atgtgaagc agcagaaaa tcgtcttgt cggcgaagg aaattgcaa aaagttaca tcctctcct attttatct ttgacgtta tttcgtcta caccgtgtc atatgatat tacattggc tgagacggg atgaggata acgtctggg aaatgctaa ctttgctga aacttgtgc ggttgccga cacttgtca attatttgc gcaaattgg gagagcggt ggaaaatga cgtccgcca tgaagttca tttgcttct tcctgtggg tgtcagttt tgttaattt ggctcacaa gtctgatct ttctgatca tgagaatgt ggcactctc gttcaatgg tcaaggcaa gaatcaaaa attttccca tgcttttca ccttctttc gaatcgtga gatgcatga tgttagtcg gcgtaaccg agaaaatga acaccagta ctgtggtga tggttttcc aaatcggcg gtttcgcaa tgggcgcta ttttacaaa ttgtgatat tatcgaaac tgatgcagt tgttccaaa agcgggcca gttccaaga gcaaataca acactctca ttgaagaaa tcggagaat atatgaaat tcaaggaac tgccaacgc agctgaaag accgcattc tgaaatacg tggacttta agttccaaa agcacattt tccgggagg aggacgtcc tgaataatt tatcctcgg tcctgaagc aggacattt tgatacaca ggtgccaga aaatggtgg agaaagtgg aatttttca aggacttgc ccactcatg tcttacttc gaattgtga caaaactac gatctgaga tttttttgc ccaatgatg tgatcgttc tggccggat atgcaggac atgcgatgt attttatct acatgggga cagtggccg tgtttacgg gacacggga aagagattt gtcacttgg aggacggtg ggcatttcg gtgaaattg ccctggtga ttaacgagc cacgtgttg ccactgtca ttgctgtga aagaatgcg agttgttca agttgagtc ggagggatt ttcaagagg cgattgagc cgtttcctg aacttaata aaaaaatac ggcagttgg caatgctca ggcttgaga gcacgctgc agattttgt cggagaata aagaggaga taagaatac gacacgagt cgatggtgg agaccgagg atgatgagt ttattgata ctatttcga tttagtttg gaaaattgt tgtaaatta aaataaatg ttgtatttt ttg
