Status
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Gene information
General information
TC033340
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015455,TC015457,TC015456
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9630888..9636133
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033340
    iBeetle screen iB_05820

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033340 RA
      MLLVVLLVL GTTLVGANL PQVTLPDLG QLEGTHGTS 
      LKGRTFHKF EGVPYARPP VGKYRFREP VPPKPWQGV 
      WQAKTLYKC MQNFQYTPP GGDFVIGDE DCLYLNIYS 
      PNIDPKANL DVIFFIHGG CFMFNYAGF QGPEYLLDR 
      DVVYVSVNY RLGPLGFLC TEDEVAPGN NGLKDQIFA 
      LQFVKKYIR YFGGNPDSV TISGMSAGG ASVHFHYLS 
      PKSRGLYHR GISQSGTML NQWVLTEKP LEKAKKLAA 
      LLGCSTESS DKMVECLKW RPGRQIVEA VKEFQPWLY 
      NPYTPFGPV VDSWAQDPV LPQHPYILI KEGKFTDLP 
      WLASYTSSE GLYPASDFY IDEQYLEDI DRRWNEIVP 
      FILDYNNTV ESQLRDEVS QKIRKYYLG DKKVSRSTF 
      MDFVKITTD RVFVDGVYN TIRMQAPLM KSPLYSYFF 
      EYRGAHSRS ESRTGTNKN FGAAHGDDT AYVFKTSVD 
      TASTENDQK VVELLVDIF TSFAKNGVP KVPLEWPPV 
      SRSTDSIIQ LKIAGPNDL SLVESKFKN GDFWRSLPL 
      MENEKLFEN RKDELEIPN HKCGLPIHG ETLSYRIHS 
      GLFVGFRRR LWKWKTVSN SIPESRTVL KSTAQIYIE 
      EQRHFHRHP CIIHPYSQA RFLWEMFMI PVFVLLFIT 
      IPVVLSNSK VNSGIVVVF YIKLGLDAI SFLDIMLNF 
      CTGYYDVKQ QKIVLSAKE IAKSYILSY FIFDVISST 
      PCHMILHWL RRDEDNVWE MLTLLKLVR LPTLVNYLR 
      KLGERWKMT SAMKFICFF LWVSVLLIW LTSLIFLIM 
      RMWHSRSMV KARIKNFPM LFTFFRIVR CMMLVGVTE 
      KMNTSTVVM VFQIGGFAM GAILQIVIL SKLMQLFQK 
      RASSKSKYN TLIEEIGEY MKFKELPTQ LKDRILKYV 
      DFKFQKHIF REEDVLNNL SSVLKQDIL IHRCQKMVE 
      KVEFFKDLP THVLLRIVT KLRSEIFLP NDVIVLAGY 
      AGHAMYFIY MGTVAVFTG HGKEICHLE DGGHFGEIA 
      LVINEPRVA TVIAVKECE LFKLSRRDF QEAIEPFPE 
      LNKKIRQLA MLRLESTLQ ILSENKEEI RIRHESMVE 
      TEDDEFIDT ISI
    CDS sequence
    • >TC033340 RA
      atgttactg gtcgtttta ctggtctta ggtaccacc 
      cttgtgggt gcaaatttg ccccaagtc acactaccc 
      gatttaggg caacttgaa ggtacccat ggtactagc 
      ctcaaaggc cgtactttt cacaagttc gagggagta 
      ccatatgca agaccacct gtcggcaag taccgtttc 
      cgggaaccg gtaccacca aaaccctgg caaggggta 
      tggcaagcc aaaactttg tacaaatgt atgcaaaac 
      ttccagtac acgccacca gggggcgac ttcgtcatt 
      ggcgatgag gattgtctt tatttgaac atttatagc 
      cccaatatt gaccctaaa gccaacttg gacgtgatt 
      tttttcata catgggggc tgttttatg tttaattat 
      gccggtttc caaggccct gagtactta ctggaccgg 
      gacgtagtt tacgtcagt gtgaactat agattaggc 
      cctttgggc tttttgtgt actgaagac gaagtggca 
      cccggaaac aacgggtta aaggaccaa attttcgcc 
      ctccaattt gtcaaaaag tacattcgg tactttggt 
      ggtaaccct gattcggtc acaatttcg ggaatgtca 
      gctgggggg gcaagtgta catttccac tacttgtcg 
      cccaaatcg cgcgggttg taccaccgg gggatttcc 
      caaagtggt accatgctc aaccagtgg gtactgacc 
      gaaaaaccg ctcgaaaaa gccaaaaaa ttggcagct 
      ttgctgggg tgtagtacc gaaagtagc gacaagatg 
      gtggagtgt ttgaaatgg cggcctggg cgacagatt 
      gtcgaagca gtgaaggag ttccagcct tggttgtac 
      aacccctac acgccgttt ggcccagtt gtggactcg 
      tgggcccaa gacccggta ctaccccaa catccttat 
      attttaatc aaggagggg aaattcacg gatttgcct 
      tggttggcg tcttatacg agttcagaa gggttgtac 
      ccagcttca gatttttac atcgatgag cagtacttg 
      gaagatatt gacaggcgt tggaacgag atagtaccc 
      tttattttg gattataac aacactgtt gagtcccag 
      ttgcgagac gaagtcagt caaaaaata cgaaagtac 
      taccttggt gataagaaa gtttcaagg agtactttc 
      atggacttt gtcaaaata accacagat cgggttttt 
      gtcgatggg gtttataac acaatccga atgcaagca 
      ccactgatg aagtcaccc ctttattcg tactttttc 
      gagtaccgg ggggcccac agtcgctcc gaaagtcgc 
      actggtacc aacaaaaat ttcggagct gcacatggt 
      gacgataca gcttatgtt ttcaaaact agtgttgac 
      acagcaagt accgaaaat gaccaaaag gtggtcgaa 
      ctcctagtt gatattttc acctctttt gccaaaaat 
      ggagtacct aaagtacca cttgaatgg cccccagtt 
      tcgagaagt accgactcg ataattcag ttaaaaatc 
      gcaggtccg aacgactta tccctagtc gagtccaag 
      tttaaaaat ggcgatttt tggcgaagt ttgccgttg 
      atggagaac gaaaaactt tttgaaaac agaaaagac 
      gaactggaa atccccaac cacaaatgt gggctccca 
      attcacggg gaaaccctg tcgtaccgc atccacagc 
      ggcttgttc gtgggcttc cgccggcgg ctgtggaaa 
      tggaaaacc gtcagcaac tcaattccg gaaagcagg 
      accgtactc aagtccacg gcccaaatt tacatcgag 
      gagcagcgc cactttcat cgccacccc tgtataatc 
      catccctac agtcaggcg cgcttcctg tgggaaatg 
      ttcatgatt cccgttttt gttctcctg ttcatcaca 
      ataccggtc gtgctctcc aactccaaa gtcaatagt 
      ggaatagtg gtggtcttc tacatcaag ctgggcctg 
      gatgccatt tccttcctg gacataatg ctcaatttc 
      tgcaccggg tactacgat gtgaagcag cagaaaatc 
      gtcttgtcg gcgaaggaa attgcaaaa agttacatc 
      ctctcctat tttatcttt gacgttatt tcgtctaca 
      ccgtgtcat atgatatta cattggctg agacgggat 
      gaggataac gtctgggaa atgctaact ttgctgaaa 
      cttgtgcgg ttgccgaca cttgtcaat tatttgcgc 
      aaattggga gagcggtgg aaaatgacg tccgccatg 
      aagttcatt tgcttcttc ctgtgggtg tcagttttg 
      ttaatttgg ctcacaagt ctgatcttt ctgatcatg 
      agaatgtgg cactctcgt tcaatggtc aaggcaaga 
      atcaaaaat tttcccatg cttttcacc ttctttcga 
      atcgtgaga tgcatgatg ttagtcggc gtaaccgag 
      aaaatgaac accagtact gtggtgatg gttttccaa 
      atcggcggt ttcgcaatg ggcgctatt ttacaaatt 
      gtgatatta tcgaaactg atgcagttg ttccaaaag 
      cgggccagt tccaagagc aaatacaac actctcatt 
      gaagaaatc ggagaatat atgaaattc aaggaactg 
      ccaacgcag ctgaaagac cgcattctg aaatacgtg 
      gactttaag ttccaaaag cacattttc cgggaggag 
      gacgtcctg aataattta tcctcggtc ctgaagcag 
      gacattttg atacacagg tgccagaaa atggtggag 
      aaagtggaa tttttcaag gacttgccc actcatgtc 
      ttacttcga attgtgaca aaactacga tctgagatt 
      tttttgccc aatgatgtg atcgttctg gccggatat 
      gcaggacat gcgatgtat tttatctac atggggaca 
      gtggccgtg tttacggga cacgggaaa gagatttgt 
      cacttggag gacggtggg catttcggt gaaattgcc 
      ctggtgatt aacgagcca cgtgttgcc actgtcatt 
      gctgtgaaa gaatgcgag ttgttcaag ttgagtcgg 
      agggatttt caagaggcg attgagccg tttcctgaa 
      cttaataaa aaaatacgg cagttggca atgctcagg 
      cttgagagc acgctgcag attttgtcg gagaataaa 
      gaggagata agaatacga cacgagtcg atggtggag 
      accgaggat gatgagttt attgatact atttcgatt 
      tag
    mRNA sequence
    • >TC033340 RA
      ctacaagcg caaagtcat ggtaccact tatcagctg 
      tcttataaa attcgtcgt cttgcgtaa ccattagta 
      ttagtgaag tcatgttac tggtcgttt tactggtct 
      taggtacca cccttgtgg gtgcaaatt tgccccaag 
      tcacactac ccgatttag ggcaacttg aaggtaccc 
      atggtacta gcctcaaag gccgtactt ttcacaagt 
      tcgagggag taccatatg caagaccac ctgtcggca 
      agtaccgtt tccgggaac cggtaccac caaaaccct 
      ggcaagggg tatggcaag ccaaaactt tgtacaaat 
      gtatgcaaa acttccagt acacgccac cagggggcg 
      acttcgtca ttggcgatg aggattgtc tttatttga 
      acatttata gccccaata ttgacccta aagccaact 
      tggacgtga tttttttca tacatgggg gctgtttta 
      tgtttaatt atgccggtt tccaaggcc ctgagtact 
      tactggacc gggacgtag tttacgtca gtgtgaact 
      atagattag gccctttgg gctttttgt gtactgaag 
      acgaagtgg cacccggaa acaacgggt taaaggacc 
      aaattttcg ccctccaat ttgtcaaaa agtacattc 
      ggtactttg gtggtaacc ctgattcgg tcacaattt 
      cgggaatgt cagctgggg gggcaagtg tacatttcc 
      actacttgt cgcccaaat cgcgcgggt tgtaccacc 
      gggggattt cccaaagtg gtaccatgc tcaaccagt 
      gggtactga ccgaaaaac cgctcgaaa aagccaaaa 
      aattggcag ctttgctgg ggtgtagta ccgaaagta 
      gcgacaaga tggtggagt gtttgaaat ggcggcctg 
      ggcgacaga ttgtcgaag cagtgaagg agttccagc 
      cttggttgt acaacccct acacgccgt ttggcccag 
      ttgtggact cgtgggccc aagacccgg tactacccc 
      aacatcctt atattttaa tcaaggagg ggaaattca 
      cggatttgc cttggttgg cgtcttata cgagttcag 
      aagggttgt acccagctt cagattttt acatcgatg 
      agcagtact tggaagata ttgacaggc gttggaacg 
      agatagtac cctttattt tggattata acaacactg 
      ttgagtccc agttgcgag acgaagtca gtcaaaaaa 
      tacgaaagt actaccttg gtgataaga aagtttcaa 
      ggagtactt tcatggact ttgtcaaaa taaccacag 
      atcgggttt ttgtcgatg gggtttata acacaatcc 
      gaatgcaag caccactga tgaagtcac ccctttatt 
      cgtactttt tcgagtacc ggggggccc acagtcgct 
      ccgaaagtc gcactggta ccaacaaaa atttcggag 
      ctgcacatg gtgacgata cagcttatg ttttcaaaa 
      ctagtgttg acacagcaa gtaccgaaa atgaccaaa 
      aggtggtcg aactcctag ttgatattt tcacctctt 
      ttgccaaaa atggagtac ctaaagtac cacttgaat 
      ggcccccag tttcgagaa gtaccgact cgataattc 
      agttaaaaa tcgcaggtc cgaacgact tatccctag 
      tcgagtcca agtttaaaa atggcgatt tttggcgaa 
      gtttgccgt tgatggaga acgaaaaac tttttgaaa 
      acagaaaag acgaactgg aaatcccca accacaaat 
      gtgggctcc caattcacg gggaaaccc tgtcgtacc 
      gcatccaca gcggcttgt tcgtgggct tccgccggc 
      ggctgtgga aatggaaaa ccgtcagca actcaattc 
      cggaaagca ggaccgtac tcaagtcca cggcccaaa 
      tttacatcg aggagcagc gccactttc atcgccacc 
      cctgtataa tccatccct acagtcagg cgcgcttcc 
      tgtgggaaa tgttcatga ttcccgttt ttgttctcc 
      tgttcatca caataccgg tcgtgctct ccaactcca 
      aagtcaata gtggaatag tggtggtct tctacatca 
      agctgggcc tggatgcca tttccttcc tggacataa 
      tgctcaatt tctgcaccg ggtactacg atgtgaagc 
      agcagaaaa tcgtcttgt cggcgaagg aaattgcaa 
      aaagttaca tcctctcct attttatct ttgacgtta 
      tttcgtcta caccgtgtc atatgatat tacattggc 
      tgagacggg atgaggata acgtctggg aaatgctaa 
      ctttgctga aacttgtgc ggttgccga cacttgtca 
      attatttgc gcaaattgg gagagcggt ggaaaatga 
      cgtccgcca tgaagttca tttgcttct tcctgtggg 
      tgtcagttt tgttaattt ggctcacaa gtctgatct 
      ttctgatca tgagaatgt ggcactctc gttcaatgg 
      tcaaggcaa gaatcaaaa attttccca tgcttttca 
      ccttctttc gaatcgtga gatgcatga tgttagtcg 
      gcgtaaccg agaaaatga acaccagta ctgtggtga 
      tggttttcc aaatcggcg gtttcgcaa tgggcgcta 
      ttttacaaa ttgtgatat tatcgaaac tgatgcagt 
      tgttccaaa agcgggcca gttccaaga gcaaataca 
      acactctca ttgaagaaa tcggagaat atatgaaat 
      tcaaggaac tgccaacgc agctgaaag accgcattc 
      tgaaatacg tggacttta agttccaaa agcacattt 
      tccgggagg aggacgtcc tgaataatt tatcctcgg 
      tcctgaagc aggacattt tgatacaca ggtgccaga 
      aaatggtgg agaaagtgg aatttttca aggacttgc 
      ccactcatg tcttacttc gaattgtga caaaactac 
      gatctgaga tttttttgc ccaatgatg tgatcgttc 
      tggccggat atgcaggac atgcgatgt attttatct 
      acatgggga cagtggccg tgtttacgg gacacggga 
      aagagattt gtcacttgg aggacggtg ggcatttcg 
      gtgaaattg ccctggtga ttaacgagc cacgtgttg 
      ccactgtca ttgctgtga aagaatgcg agttgttca 
      agttgagtc ggagggatt ttcaagagg cgattgagc 
      cgtttcctg aacttaata aaaaaatac ggcagttgg 
      caatgctca ggcttgaga gcacgctgc agattttgt 
      cggagaata aagaggaga taagaatac gacacgagt 
      cgatggtgg agaccgagg atgatgagt ttattgata 
      ctatttcga tttagtttg gaaaattgt tgtaaatta 
      aaataaatg ttgtatttt ttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY