Status
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Gene information
General information
TC033338
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015449,TC015448
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9674511..9680334
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033338
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05819

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033338 RA
      MSLNLADVK CEYSKNPTL KQEDVKSLM EWVNKQPHL 
      PKINELQAI LFLQSCYNR NEAAKAAID TFFTVKTLC 
      SDLFGGRNP LTSPNKDTM NVSLMTPLP RETPEGHCI 
      FLFKLIDTN PDKYIFGDQ LRCFDMMIM RHLHQCGTS 
      NGHLILFDM KGVVFGHVT KLGPLNMKK FLYYLQEAM 
      PVRLKGLHF FNIVPFMDK ILALMKPFM KKEMIDMLY 
      LHNTVDDLV KQVPRDCLP QEYGGSAES MTILHEKMK 
      RTLCENSAF FEWEDGQKV DESLRPGKP KNVGDYFGV 
      EGTFKKLEL D
    • >TC033338 RB
      MSFDLMDVE KEYAKDNRL SPQDVKILA DWMEKQPHL 
      PKATELQLI LFLQSCYYR IEVAKNAID NFFTVRTLC 
      PDIFGAPPI EILKRELTV GSINFLPEQ TPEGYRVVL 
      MKLIDTRPE HYHCVSEIN FTDLTLMLN LHQQGTCNG 
      LVAIMDMKG LTFGHLTRF NLVAIKKHL FYVQEAVPV 
      RIKGIHHIN IVPFTDKII AMVKPFMKK ELIDLLHFH 
      NSMDSLFKF VPKHVLPKD YGGEAPSLE TLHEQNAKN 
      LLENMDFFK WMDTLKVDE TKRPGKPKN AGSIFGVDG 
      TFKKLEVD
    • >TC033338 RC
      MSLNLADVK CEYSKNPTL KQEDVKSLM EWVNKQPHL 
      PKINELQAI LFLQSCYNR NEAAKAAID TFFTVKTLC 
      SDLFGGRNP LTSPNKDTM NVSLMTPLP RETPEGHCI 
      FLFKLIDTN PDKYIFGDQ LRCFDMMIM RHLHQCGTS 
      NGHLILFDM KGVVFGHVT KLGPLNMKK FLYYLQEAM 
      PVRLKGLHF FNIVPFMDK ILALMKPFM KKEMIDMLY 
      LHNTVDDLV KQVPRDCLP QEYGGSAES MTILHEKMK 
      RTLCENSAF FEWEDGQKV DESLRPGKP KNVGDYFGV 
      EGTFKNLGL TTGRAPPKT ARMSFDLMD VEKEYAKDN 
      RLSPQDVKI LADWMEKQP HLPKATELQ LILFLQSCY 
      YRIEVAKNA IDNFFTVRT LCPDIFGAP PIEILKREL 
      TVGSINFLP EQTPEGYRV VLMKLIDTR PEHYHCVSE 
      INFTDLTLM LNLHQQGTC NGLVAIMDM KGLTFGHLT 
      RFNLVAIKK HLFYVQEAV PVRIKGIHH INIVPFTDK 
      IIAMVKPFM KKELIDLLH FHNSMDSLF KFVPKHVLP 
      KDYGGEAPS LETLHEQNA KNLLENMDF FKWMDTLKV 
      DETKRPGKP KNAGSIFGV DGTFKKLEV D
    CDS sequence
    • >TC033338 RA
      atgtcgctt aatttagcc gacgttaag tgcgaatat 
      tcaaaaaac ccaacattg aaacaagaa gatgtgaaa 
      tcgctaatg gaatgggtt aacaaacaa ccccatctt 
      ccgaaaatt aacgaattg caagcgatt ttattcctc 
      caaagttgt tacaaccga aacgaggct gcgaaagca 
      gccattgac acttttttc accgttaaa accctctgt 
      tctgactta ttcgggggg agaaacccc ctaacatcc 
      cccaataaa gacacaatg aacgtctca cttatgaca 
      cccctccct cgggaaacc cccgaaggt cattgcatt 
      tttctcttc aaattgatt gacaccaac cctgataag 
      tacattttc ggcgaccaa ttgcgctgt tttgacatg 
      atgatcatg cggcatttg caccaatgt ggcacttcc 
      aacggccac ttgatcctc tttgacatg aaaggcgtg 
      gtttttggg cacgtgact aaactgggc cctttgaat 
      atgaagaaa tttttgtac tatcttcag gaggcaatg 
      cctgtaagg ctcaaaggg ttgcacttt ttcaatatt 
      gtccctttc atggacaaa atcctagcc ttgatgaag 
      cccttcatg aagaaagaa atgatagat atgttgtat 
      ttgcacaac actgtggac gacttggtc aagcaagtg 
      ccccgcgat tgtttgccc caggaatac ggaggttcg 
      gctgaaagc atgacgatt ttgcacgaa aaaatgaaa 
      cggacgttg tgcgaaaat tctgcgttt tttgaatgg 
      gaggatggg caaaaggtt gatgagagt ttaaggccc 
      ggaaagccg aaaaatgtg ggtgattat ttcggagtt 
      gagggtact tttaaaaag ttggaatta gattaa
    • >TC033338 RB
      atgtctttc gatctgatg gacgttgaa aaagagtac 
      gccaaagac aaccggctc agcccccag gacgtgaaa 
      atcctcgcc gactggatg gaaaaacag ccccatttg 
      cccaaagcc accgaactc cagctcatc cttttcctc 
      cagagttgt tactacaga atcgaggtt gcgaaaaat 
      gcaatcgat aattttttc acagttcga actctatgt 
      cccgacatt ttcggggct ccaccaatc gaaatatta 
      aaacgtgaa ttaacagtt ggttcgatt aatttcctc 
      cctgagcaa acaccagag ggctatagg gtggtttta 
      atgaagttg attgacaca cgacctgaa cattaccac 
      tgtgtgagt gagataaat ttcacggac ttgacattg 
      atgctgaat ttgcaccaa caaggcact tgtaacgga 
      cttgtggcc attatggac atgaaaggg ctcactttt 
      gggcacctg actaggttt aatttggtg gcgattaag 
      aaacacttg ttctatgtt caagaagcc gtccctgtc 
      cgaattaag ggcatacac cacattaat attgtccct 
      ttcactgat aaaatcata gccatggtc aagcccttc 
      atgaagaag gaactaatc gatttgctc cattttcac 
      aattctatg gattcgttg tttaaattt gtccctaag 
      catgttttg cctaaggat tatgggggc gaagctccg 
      tcgcttgaa acattgcac gaacaaaac gccaaaaat 
      cttttggaa aacatggat ttttttaaa tggatggat 
      acgctcaaa gttgacgag actaaaagg cccgggaaa 
      ccgaaaaat gctggaagt attttcggt gttgatggt 
      actttcaaa aaattggaa gttgattaa
    • >TC033338 RC
      atgtcgctt aatttagcc gacgttaag tgcgaatat 
      tcaaaaaac ccaacattg aaacaagaa gatgtgaaa 
      tcgctaatg gaatgggtt aacaaacaa ccccatctt 
      ccgaaaatt aacgaattg caagcgatt ttattcctc 
      caaagttgt tacaaccga aacgaggct gcgaaagca 
      gccattgac acttttttc accgttaaa accctctgt 
      tctgactta ttcgggggg agaaacccc ctaacatcc 
      cccaataaa gacacaatg aacgtctca cttatgaca 
      cccctccct cgggaaacc cccgaaggt cattgcatt 
      tttctcttc aaattgatt gacaccaac cctgataag 
      tacattttc ggcgaccaa ttgcgctgt tttgacatg 
      atgatcatg cggcatttg caccaatgt ggcacttcc 
      aacggccac ttgatcctc tttgacatg aaaggcgtg 
      gtttttggg cacgtgact aaactgggc cctttgaat 
      atgaagaaa tttttgtac tatcttcag gaggcaatg 
      cctgtaagg ctcaaaggg ttgcacttt ttcaatatt 
      gtccctttc atggacaaa atcctagcc ttgatgaag 
      cccttcatg aagaaagaa atgatagat atgttgtat 
      ttgcacaac actgtggac gacttggtc aagcaagtg 
      ccccgcgat tgtttgccc caggaatac ggaggttcg 
      gctgaaagc atgacgatt ttgcacgaa aaaatgaaa 
      cggacgttg tgcgaaaat tctgcgttt tttgaatgg 
      gaggatggg caaaaggtt gatgagagt ttaaggccc 
      ggaaagccg aaaaatgtg ggtgattat ttcggagtt 
      gagggtact tttaaaaat ttaggtttg acaacagga 
      cgtgcacca ccgaaaact gcaagaatg tctttcgat 
      ctgatggac gttgaaaaa gagtacgcc aaagacaac 
      cggctcagc ccccaggac gtgaaaatc ctcgccgac 
      tggatggaa aaacagccc catttgccc aaagccacc 
      gaactccag ctcatcctt ttcctccag agttgttac 
      tacagaatc gaggttgcg aaaaatgca atcgataat 
      tttttcaca gttcgaact ctatgtccc gacattttc 
      ggggctcca ccaatcgaa atattaaaa cgtgaatta 
      acagttggt tcgattaat ttcctccct gagcaaaca 
      ccagagggc tatagggtg gttttaatg aagttgatt 
      gacacacga cctgaacat taccactgt gtgagtgag 
      ataaatttc acggacttg acattgatg ctgaatttg 
      caccaacaa ggcacttgt aacggactt gtggccatt 
      atggacatg aaagggctc acttttggg cacctgact 
      aggtttaat ttggtggcg attaagaaa cacttgttc 
      tatgttcaa gaagccgtc cctgtccga attaagggc 
      atacaccac attaatatt gtccctttc actgataaa 
      atcatagcc atggtcaag cccttcatg aagaaggaa 
      ctaatcgat ttgctccat tttcacaat tctatggat 
      tcgttgttt aaatttgtc cctaagcat gttttgcct 
      aaggattat gggggcgaa gctccgtcg cttgaaaca 
      ttgcacgaa caaaacgcc aaaaatctt ttggaaaac 
      atggatttt tttaaatgg atggatacg ctcaaagtt 
      gacgagact aaaaggccc gggaaaccg aaaaatgct 
      ggaagtatt ttcggtgtt gatggtact ttcaaaaaa 
      ttggaagtt gattaa
    mRNA sequence
    • >TC033338 RA
      tagctgcaa caattgtcc gcctgataa accaaaaaa 
      accatttgt atcgtcaca atagcgaac acacgaaac 
      ctcaatacc aaataaaat tacaacatg aaacattct 
      gttggtttg ttggtgctt ttagctact actttggca 
      gcatccatg tatttacaa aataattta aaccaacat 
      caaaaaata cccacttgt ttttggatt gttatctac 
      agacgagcc ccaactcgg gtgacacca ccaccagtc 
      agttttatt tcaactcta gagcacgtt aaattaaaa 
      aaaaaatgt cgcttaatt tagccgacg ttaagtgcg 
      aatattcaa aaaacccaa cattgaaac aagaagatg 
      tgaaatcgc taatggaat gggttaaca aacaacccc 
      atcttccga aaattaacg aattgcaag cgattttat 
      tcctccaaa gttgttaca accgaaacg aggctgcga 
      aagcagcca ttgacactt ttttcaccg ttaaaaccc 
      tctgttctg acttattcg gggggagaa accccctaa 
      catccccca ataaagaca caatgaacg tctcactta 
      tgacacccc tccctcggg aaacccccg aaggtcatt 
      gcatttttc tcttcaaat tgattgaca ccaaccctg 
      ataagtaca ttttcggcg accaattgc gctgttttg 
      acatgatga tcatgcggc atttgcacc aatgtggca 
      cttccaacg gccacttga tcctctttg acatgaaag 
      gcgtggttt ttgggcacg tgactaaac tgggccctt 
      tgaatatga agaaatttt tgtactatc ttcaggagg 
      caatgcctg taaggctca aagggttgc actttttca 
      atattgtcc ctttcatgg acaaaatcc tagccttga 
      tgaagccct tcatgaaga aagaaatga tagatatgt 
      tgtatttgc acaacactg tggacgact tggtcaagc 
      aagtgcccc gcgattgtt tgccccagg aatacggag 
      gttcggctg aaagcatga cgattttgc acgaaaaaa 
      tgaaacgga cgttgtgcg aaaattctg cgttttttg 
      aatgggagg atgggcaaa aggttgatg agagtttaa 
      ggcccggaa agccgaaaa atgtgggtg attatttcg 
      gagttgagg gtactttta aaaagttgg aattagatt 
      aatgtcatt gtggaagta gataattat atgacatgc 
      atcattgta aa
    • >TC033338 RB
      gtttaggtt tgacaacag gacgtgcac caccgaaaa 
      ctgcaagaa tgtctttcg atctgatgg acgttgaaa 
      aagagtacg ccaaagaca accggctca gcccccagg 
      acgtgaaaa tcctcgccg actggatgg aaaaacagc 
      cccatttgc ccaaagcca ccgaactcc agctcatcc 
      ttttcctcc agagttgtt actacagaa tcgaggttg 
      cgaaaaatg caatcgata attttttca cagttcgaa 
      ctctatgtc ccgacattt tcggggctc caccaatcg 
      aaatattaa aacgtgaat taacagttg gttcgatta 
      atttcctcc ctgagcaaa caccagagg gctataggg 
      tggttttaa tgaagttga ttgacacac gacctgaac 
      attaccact gtgtgagtg agataaatt tcacggact 
      tgacattga tgctgaatt tgcaccaac aaggcactt 
      gtaacggac ttgtggcca ttatggaca tgaaagggc 
      tcacttttg ggcacctga ctaggttta atttggtgg 
      cgattaaga aacacttgt tctatgttc aagaagccg 
      tccctgtcc gaattaagg gcatacacc acattaata 
      ttgtccctt tcactgata aaatcatag ccatggtca 
      agcccttca tgaagaagg aactaatcg atttgctcc 
      attttcaca attctatgg attcgttgt ttaaatttg 
      tccctaagc atgttttgc ctaaggatt atgggggcg 
      aagctccgt cgcttgaaa cattgcacg aacaaaacg 
      ccaaaaatc ttttggaaa acatggatt tttttaaat 
      ggatggata cgctcaaag ttgacgaga ctaaaaggc 
      ccgggaaac cgaaaaatg ctggaagta ttttcggtg 
      ttgatggta ctttcaaaa aattggaag ttgattaat 
      tatgttatt aattgttga ttgtgtgtt ttatgggtt 
      aataaaaat attaatggt aa
    • >TC033338 RC
      tagctgcaa caattgtcc gcctgataa accaaaaaa 
      accatttgt atcgtcaca atagcgaac acacgaaac 
      ctcaatacc aaataaaat tacaacatg aaacattct 
      gttggtttg ttggtgctt ttagctact actttggca 
      gcatccatg tatttacaa aataattta aaccaacat 
      caaaaaata cccacttgt ttttggatt gttatctac 
      agacgagcc ccaactcgg gtgacacca ccaccagtc 
      agttttatt tcaactcta gagcacgtt aaattaaaa 
      aaaaaatgt cgcttaatt tagccgacg ttaagtgcg 
      aatattcaa aaaacccaa cattgaaac aagaagatg 
      tgaaatcgc taatggaat gggttaaca aacaacccc 
      atcttccga aaattaacg aattgcaag cgattttat 
      tcctccaaa gttgttaca accgaaacg aggctgcga 
      aagcagcca ttgacactt ttttcaccg ttaaaaccc 
      tctgttctg acttattcg gggggagaa accccctaa 
      catccccca ataaagaca caatgaacg tctcactta 
      tgacacccc tccctcggg aaacccccg aaggtcatt 
      gcatttttc tcttcaaat tgattgaca ccaaccctg 
      ataagtaca ttttcggcg accaattgc gctgttttg 
      acatgatga tcatgcggc atttgcacc aatgtggca 
      cttccaacg gccacttga tcctctttg acatgaaag 
      gcgtggttt ttgggcacg tgactaaac tgggccctt 
      tgaatatga agaaatttt tgtactatc ttcaggagg 
      caatgcctg taaggctca aagggttgc actttttca 
      atattgtcc ctttcatgg acaaaatcc tagccttga 
      tgaagccct tcatgaaga aagaaatga tagatatgt 
      tgtatttgc acaacactg tggacgact tggtcaagc 
      aagtgcccc gcgattgtt tgccccagg aatacggag 
      gttcggctg aaagcatga cgattttgc acgaaaaaa 
      tgaaacgga cgttgtgcg aaaattctg cgttttttg 
      aatgggagg atgggcaaa aggttgatg agagtttaa 
      ggcccggaa agccgaaaa atgtgggtg attatttcg 
      gagttgagg gtactttta aaaatttag gtttgacaa 
      caggacgtg caccaccga aaactgcaa gaatgtctt 
      tcgatctga tggacgttg aaaaagagt acgccaaag 
      acaaccggc tcagccccc aggacgtga aaatcctcg 
      ccgactgga tggaaaaac agccccatt tgcccaaag 
      ccaccgaac tccagctca tccttttcc tccagagtt 
      gttactaca gaatcgagg ttgcgaaaa atgcaatcg 
      ataattttt tcacagttc gaactctat gtcccgaca 
      ttttcgggg ctccaccaa tcgaaatat taaaacgtg 
      aattaacag ttggttcga ttaatttcc tccctgagc 
      aaacaccag agggctata gggtggttt taatgaagt 
      tgattgaca cacgacctg aacattacc actgtgtga 
      gtgagataa atttcacgg acttgacat tgatgctga 
      atttgcacc aacaaggca cttgtaacg gacttgtgg 
      ccattatgg acatgaaag ggctcactt ttgggcacc 
      tgactaggt ttaatttgg tggcgatta agaaacact 
      tgttctatg ttcaagaag ccgtccctg tccgaatta 
      agggcatac accacatta atattgtcc ctttcactg 
      ataaaatca tagccatgg tcaagccct tcatgaaga 
      aggaactaa tcgatttgc tccattttc acaattcta 
      tggattcgt tgtttaaat ttgtcccta agcatgttt 
      tgcctaagg attatgggg gcgaagctc cgtcgcttg 
      aaacattgc acgaacaaa acgccaaaa atcttttgg 
      aaaacatgg attttttta aatggatgg atacgctca 
      aagttgacg agactaaaa ggcccggga aaccgaaaa 
      atgctggaa gtattttcg gtgttgatg gtactttca 
      aaaaattgg aagttgatt aattatgtt attaattgt 
      tgattgtgt gttttatgg gttaataaa aatattaat 
      ggtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY