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Gene information
General information
TC015447 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9679860..9682353
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015447
    iBeetle screen iB_05818

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015447 RA
      MALNFFDPK TELTKDKNL REDDIKTLI DWVNKQAHL 
      PKINEVQAI LFLQSCNYR NEAAKSAID SYFTVKTFC 
      ADLFGGRNP LQSPLRESL NVALFAFLP KKTPQGDTI 
      IFMKLMDSN PDNYNFSVQ LKHFDMVCM LGLHQEGVS 
      NGHIIVLDM KEVVFGHLT KLGPLAMKK FLYYLQEAM 
      PLRLKGLHY FNIVPFMDK ILALMRPFM KKELLDMLY 
      LHNTVDDLV KFVPRECLP LDYGGSVES THVIHEKTK 
      NKLRENCAF FDWEDKQVV DETTTLAAS MYLQNNLNQ 
      HQKIPTCFW IVIYRRAPT RVTPPPVMP VQPPVVTTQ 
      SGIDERNTE TSTVAEETT IEETTEATT EAPTE
    CDS sequence
    • >TC015447 RA
      atggcactc aattttttc gacccgaaa accgaactc 
      accaaagac aaaaatcta cgagaggac gacatcaaa 
      acattgatt gattgggtc aacaaacag gcacatttg 
      cccaaaatc aacgaggta caagcgatt ttgttcctc 
      caaagctgc aactaccga aatgaagcg gccaaaagt 
      gcaattgat tcgtatttt actgtgaaa acgttttgt 
      gccgattta ttcggaggt cgcaaccct ttacagtca 
      ccccttaga gaaagtcta aatgttgca ctttttgct 
      tttttgccc aaaaaaacg ccccaaggc gacacgatt 
      attttcatg aagttgatg gattcaaac ccggacaat 
      tacaatttt tccgtacaa ttgaaacac tttgatatg 
      gtctgtatg ttgggcttg catcaggaa ggagtttca 
      aatggtcat ataattgtc ctggatatg aaggaagtg 
      gtttttggg catttgacc aaacttggg cctctggcc 
      atgaaaaaa tttttgtac tatttgcag gaagcaatg 
      cctttgagg ctcaaaggg ctgcattat tttaatatt 
      gtgcccttt atggacaaa attctggct ttgatgcgg 
      ccctttatg aagaaggag ctcctagat atgctttat 
      ttgcacaac actgtggat gatttggtt aagtttgta 
      ccaagggag tgtctacca cttgattat ggtggttca 
      gttgaatca actcatgtc atacatgag aaaactaaa 
      aataagtta cgtgaaaat tgtgcattt ttcgattgg 
      gaggataaa caagttgtg gatgagact actactttg 
      gcagcatcc atgtattta caaaataat ttaaaccaa 
      catcaaaaa atacccact tgtttttgg attgttatc 
      tacagacga gccccaact cgggtgaca ccaccacca 
      gtgatgcct gtgcaacca cctgttgtg acaactcag 
      tcaggtata gatgaaaga aatacagag acttcaaca 
      gtggctgag gaaaccacc attgaagaa accacagaa 
      gcgacaaca gaagcacca actgagtaa
    mRNA sequence
    • >TC015447 RA
      acttaactc caacaatgg cactcaatt ttttcgacc 
      cgaaaaccg aactcacca aagacaaaa atctacgag 
      aggacgaca tcaaaacat tgattgatt gggtcaaca 
      aacaggcac atttgccca aaatcaacg aggtacaag 
      cgattttgt tcctccaaa gctgcaact accgaaatg 
      aagcggcca aaagtgcaa ttgattcgt attttactg 
      tgaaaacgt tttgtgccg atttattcg gaggtcgca 
      accctttac agtcacccc ttagagaaa gtctaaatg 
      ttgcacttt ttgcttttt tgcccaaaa aaacgcccc 
      aaggcgaca cgattattt tcatgaagt tgatggatt 
      caaacccgg acaattaca atttttccg tacaattga 
      aacactttg atatggtct gtatgttgg gcttgcatc 
      aggaaggag tttcaaatg gtcatataa ttgtcctgg 
      atatgaagg aagtggttt ttgggcatt tgaccaaac 
      ttgggcctc tggccatga aaaaatttt tgtactatt 
      tgcaggaag caatgcctt tgaggctca aagggctgc 
      attatttta atattgtgc cctttatgg acaaaattc 
      tggctttga tgcggccct ttatgaaga aggagctcc 
      tagatatgc tttatttgc acaacactg tggatgatt 
      tggttaagt ttgtaccaa gggagtgtc taccacttg 
      attatggtg gttcagttg aatcaactc atgtcatac 
      atgagaaaa ctaaaaata agttacgtg aaaattgtg 
      catttttcg attgggagg ataaacaag ttgtggatg 
      agactacta ctttggcag catccatgt atttacaaa 
      ataatttaa accaacatc aaaaaatac ccacttgtt 
      tttggattg ttatctaca gacgagccc caactcggg 
      tgacaccac caccagtga tgcctgtgc aaccacctg 
      ttgtgacaa ctcagtcag gtatagatg aaagaaata 
      cagagactt caacagtgg ctgaggaaa ccaccattg 
      aagaaacca cagaagcga caacagaag caccaactg 
      agtaatatt gtaaataaa aataatatt aaagtttta 
      tgaaaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY