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Gene information
General information
TC015411 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 539376..541343
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015411
    iBeetle screen iB_05814

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015411 RA
      MCHKPAKRQ NAHLPGRGP QNPAPNKNP KLSLPHCLR 
      ERLFTQSRL LQMPPNARS LLFVPARVR QFREHALKL 
      FQKFQVYIK DTATKTAPP EPPTFTEVK PVENVPFYT 
      LQLPAIVTN QPVKAEKIA PMMGAIKTA PRNEMSNVV 
      VNANGEVLN IAQLVDFES LINQAGLQQ QKKCVTKAT 
      KKDKEDPII KIDLTTTSE SNYDTGQKN KNPNKFAYA 
      IKVEEKLNN PSVIFNNLP EFNQNAAFN GYNNSLYTP 
      GVVATPSVV CNTVEPSTV NLNVLSQDL NQPAAQPYQ 
      HKVVAEEMA PTQVNNNNQ IIKSHVCEI CQKSFKRRE 
      HLYQHVKLH TGFRPYTCE NCNKSFMRK EHLLRHMTS 
      HSGQKNFTC NICDKSFSR NDNLLKHKK THDKQASYT 
      CDICQKQFV MKHYYLAHK LTHDSDRCS LANVWNVLK 
      T
    CDS sequence
    • >TC015411 RA
      atgtgccac aaaccagca aaaagacaa aacgcacat 
      cttccagga agagggccg caaaatcca gctccaaac 
      aaaaatcca aagctgtct ctccctcac tgtctgcga 
      gaacgactt tttacccaa agtcgtttg ctccaaatg 
      cctccgaac gctcgaagt ttgctattc gttccggca 
      agagtgcgt cagttccga gagcatgct ctcaagcta 
      tttcaaaaa tttcaggtc tacatcaag gacacggcc 
      accaagacc gcgccccct gagccccca accttcacc 
      gaagtcaaa ccggtggaa aacgtgccc ttctacacc 
      ctacaacta ccggcaatt gtgaccaat caaccggtc 
      aaagcggaa aaaatcgcc cccatgatg ggggccatc 
      aaaacagcc ccccgaaac gaaatgagc aacgtggtg 
      gtgaacgcc aacggcgaa gtcctaaat atcgcccaa 
      ttggttgat tttgaatcg ctgattaat caagccggc 
      ttgcaacaa caaaagaaa tgcgtcacc aaggcgact 
      aagaaagac aaggaggac ccgattatt aaaattgat 
      ctcacaacg accagcgaa tcaaattat gacacgggt 
      caaaaaaat aaaaatcca aataaattc gcatacgcg 
      atcaaagtc gaagaaaaa ttaaataat ccatcggtg 
      attttcaat aatttgccc gaatttaat caaaatgct 
      gcgttcaat ggttataat aatagtctg tacacccct 
      ggtgttgtg gcgacacct agcgttgtt tgtaataca 
      gtggagcca tctactgtt aatttgaac gtattaagc 
      caggatttg aaccagcca gcggcgcag ccctaccaa 
      cataaagtg gtggccgaa gaaatggcc ccaactcag 
      gtgaataac aacaatcaa ataatcaag tcgcacgtg 
      tgtgagatt tgtcagaag agtttcaaa agaagggaa 
      catttgtac caacatgtt aagttacat acgggtttt 
      cgtccgtac acttgcgag aattgtaat aagagtttt 
      atgcgcaag gagcatctc ctaaggcat atgacgtca 
      cacagtggg cagaaaaat tttacgtgt aatatttgc 
      gataagagt ttttcgcgc aatgataat ttgctgaag 
      cataagaaa acgcacgat aaacaggcg agttatacg 
      tgcgatatt tgccagaag cagtttgtg atgaagcac 
      tattatctg gcgcataaa ttgacgcat gatagcgat 
      aggtgtagt ctggcgaat gtttggaat gtgttaaag 
      acgtag
    mRNA sequence
    • >TC015411 RA
      tgttatcac aaattgtgt gtttgtgtg cgttttttg 
      tgtcttttt gccaatttt atcagtttc gtaatgaca 
      ctgataatg gcatagtgt gctgctgtc agcgtgata 
      ttctaggtt aggtaagtg cccccttta tcacaaatt 
      gtgcgtttt tgtgttaaa tcattgttt tttgttgtg 
      atttgaaac agtttagtt gtggcgtcc gtcgttagg 
      ggcgctggc gcatcggcg tcgtcagct gtcatttaa 
      aaattgaca tttatgtaa ataatcatc aataaagcc 
      tctataaat gtaaaaaac ggtcatgtc gggctacaa 
      acgcgtcaa tttctacga attgtgtcg attatgtgc 
      cacaaacca gcaaaaaga caaaacgca catcttcca 
      ggaagaggg ccgcaaaat ccagctcca aacaaaaat 
      ccaaagctg tctctccct cactgtctg cgagaacga 
      ctttttacc caaagtcgt ttgctccaa atgcctccg 
      aacgctcga agtttgcta ttcgttccg gcaagagtg 
      cgtcagttc cgagagcat gctctcaag ctatttcaa 
      aaatttcag gtctacatc aaggacacg gccaccaag 
      accgcgccc cctgagccc ccaaccttc accgaagtc 
      aaaccggtg gaaaacgtg cccttctac accctacaa 
      ctaccggca attgtgacc aatcaaccg gtcaaagcg 
      gaaaaaatc gcccccatg atgggggcc atcaaaaca 
      gccccccga aacgaaatg agcaacgtg gtggtgaac 
      gccaacggc gaagtccta aatatcgcc caattggtt 
      gattttgaa tcgctgatt aatcaagcc ggcttgcaa 
      caacaaaag aaatgcgtc accaaggcg actaagaaa 
      gacaaggag gacccgatt attaaaatt gatctcaca 
      acgaccagc gaatcaaat tatgacacg ggtcaaaaa 
      aataaaaat ccaaataaa ttcgcatac gcgatcaaa 
      gtcgaagaa aaattaaat aatccatcg gtgattttc 
      aataatttg cccgaattt aatcaaaat gctgcgttc 
      aatggttat aataatagt ctgtacacc cctggtgtt 
      gtggcgaca cctagcgtt gtttgtaat acagtggag 
      ccatctact gttaatttg aacgtatta agccaggat 
      ttgaaccag ccagcggcg cagccctac caacataaa 
      gtggtggcc gaagaaatg gccccaact caggtgaat 
      aacaacaat caaataatc aagtcgcac gtgtgtgag 
      atttgtcag aagagtttc aaaagaagg gaacatttg 
      taccaacat gttaagtta catacgggt tttcgtccg 
      tacacttgc gagaattgt aataagagt tttatgcgc 
      aaggagcat ctcctaagg catatgacg tcacacagt 
      gggcagaaa aattttacg tgtaatatt tgcgataag 
      agtttttcg cgcaatgat aatttgctg aagcataag 
      aaaacgcac gataaacag gcgagttat acgtgcgat 
      atttgccag aagcagttt gtgatgaag cactattat 
      ctggcgcat aaattgacg catgatagc gataggtgt 
      agtctggcg aatgtttgg aatgtgtta aagacgtag 
      aaaaaagct ttaattgta taatctcaa ggtacaaaa 
      cttagtatt gtttttatt tatttattt attaggtgc 
      gtaacgacc aatgaaatt aaaaagcta gtcctttta 
      tttattact atcataagg ttttttttg taaatatga 
      tgtgtttga gtttaataa actagactg aaaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY