Status
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Gene information
General information
TC015407 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 496881..498877
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015407
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05813

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    ovary size decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late decreased - Number of animals showing the phenotype: 4

    egg chamber sometimes potentiallyfused - Number of animals showing the phenotype: 1

    vitellogenic egg chamber sometimes degenerated - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015407 RA
      MFCVKLSCE VWLVILGAL VAVLLRACT TLHPYSGAG 
SPPMYGDYE AQRHWMEIT TNLRPLEWY KNTTDNDLM 
YWGLDYPPL TAYHMYLTG KIGSFLNEN WTKLHESRG 
FEGESHKIF MRYTVLAAD IVMYIPALI FYFHAMGGQ 
SPDPKTRPL SPSLATILG LLYPGIILI DHGHFQYNC 
ISLGLLVLA TSCLLLDMN LTAAIFFTS ALNYKQMEL 
YHALPFFMY MLSSCVPKP GQSLTSSLI KLAQIAITV 
MAVFAILWA PFNRDFFAV LRRLFPLDR GVFEDKVSN 
FWCVFNIFY KLKLKFGNY EMMRICLFA TVSMVFPSS 
VDLFLRPSR KKFVLSLIN SSLAFFLFS YQVHEKSIL 
LVGVPVVLY FPQKPFVCF WFLCISVFS MLPLLYKDG 
LIIATIALM LFYVISFRV SIEHFYRSS LNASDGLAE 
YYRSLLHVL LDIEYKQAT QIDILKLTY KQIVKNKET 
LKNLAFHVV LVTSLIGCL VLSFALLVF EPPERYPDL 
FPLLISAYS ALHFFGFFL YFNVKQLQI PQEFDAIKN 
VKIKSS
    CDS sequence
    • >TC015407 RA
      atgttttgc gtgaaatta agttgtgaa gtgtggtta 
gtgattctg ggggccctt gtggccgtg ttactgcgc 
gcgtgcact actctccac ccgtattcg ggcgccggc 
agcccccca atgtacggc gattacgaa gcgcagcgc 
cattggatg gaaatcacc acgaacttg cgccccctt 
gagtggtac aagaacacc acagacaac gacctcatg 
tactggggc ctcgactac ccccctctc acagcctac 
cacatgtac ttaaccgga aaaatcggc agttttttg 
aacgaaaac tggacgaaa ttgcacgag tccagaggc 
tttgagggg gaatcccac aagattttc atgcgctat 
accgtgcta gcggccgac atagtcatg tacatcccc 
gccctgatt ttctatttc cacgccatg ggggggcaa 
agccccgac cccaaaacc aggcctttg tcgccgtcc 
ctcgccacc attctgggg ctcctctac cccggaatc 
atcctcatc gatcacgga cacttccag tataattgc 
atctctctc ggactcctt gttctggca actagctgt 
ctccttctg gacatgaac ttgaccgct gctatcttc 
ttcactagt gcactcaac tacaaacag atggagctc 
taccacgcg ttgccgttt ttcatgtac atgttgagc 
tcttgtgtc ccaaagccg ggccaaagc ctcacttcc 
agtctgatc aagttggca cagatcgcc atcactgtc 
atggcggtg ttcgccatc ttgtgggcg ccatttaat 
cgcgacttt ttcgccgtc ttgcgccgt ttatttccg 
ctggatcgc ggcgttttc gaggacaaa gtctcgaat 
ttttggtgc gtttttaac attttctac aaattgaag 
ctaaagttt ggtaattac gaaatgatg cgtatttgt 
ttgtttgcc accgtctcc atggtgttt ccgtcaagt 
gtcgacttg tttttgcgt ccgagtcgc aaaaagttt 
gttttgtcg ttgattaat tcctcactg gcgtttttc 
ttgttcagt tatcaagtg cacgagaag agtatttta 
ctagttggc gttcctgtg gtgctgtat ttcccccaa 
aaaccgttc gtttgtttc tggtttttg tgcatttcc 
gtctttagc atgttgccg ttgttgtac aaagacggt 
ctgattatt gcaacaatt gcgctaatg cttttttac 
gtcatttcc ttccgtgtt tcaattgaa cacttctat 
aggagttcc ctaaatgcg tccgacggt ttggccgaa 
tattaccga agtcttttg cacgtcttg ctcgatatt 
gagtacaaa caagcaaca cagatcgat attttgaaa 
ctgacgtac aaacaaatt gtaaaaaat aaagaaact 
ctgaaaaat ttggcgttt catgttgtg ttagtgacg 
tcgttgatt ggttgtttg gtgctttcg tttgcactg 
ttagttttt gaaccgcct gagcgttat cccgatttg 
ttcccactt ttgatttcg gcttattcg gctttacat 
tttttcgga ttttttctg tatttcaat gttaaacaa 
ctacaaata ccgcaagag tttgatgcg attaaaaat 
gttaaaatc aagtcgtct tga
    mRNA sequence
    • >TC015407 RA
      aatcgtaac aaaaaaaaa ttcaaaaaa cttcttgat 
ttgcacaac tgcaccaaa caaacaatt tcgcaactc 
ccaacccta aaacccacc aaattcacc cccatttca 
tgccaccac cactccgtc tgtcatctg ccaaaaaac 
cccccatca acggaatat cccgtttgt tggcccctt 
tctccctga aaatgcctc aatacacca gcgaccaca 
tccgcacca aactcctcc aagaactgt ccgcctcac 
acgtggtaa gtttcccaa ctttaattt ttttaataa 
caaaactgt caaaaaaaa ccctaacct cacaatcac 
caccaccat gttttgcgt gaaattaag ttgtgaagt 
gtggttagt gattctggg ggcccttgt ggccgtgtt 
actgcgcgc gtgcactac tctccaccc gtattcggg 
cgccggcag ccccccaat gtacggcga ttacgaagc 
gcagcgcca ttggatgga aatcaccac gaacttgcg 
cccccttga gtggtacaa gaacaccac agacaacga 
cctcatgta ctggggcct cgactaccc ccctctcac 
agcctacca catgtactt aaccggaaa aatcggcag 
ttttttgaa cgaaaactg gacgaaatt gcacgagtc 
cagaggctt tgaggggga atcccacaa gattttcat 
gcgctatac cgtgctagc ggccgacat agtcatgta 
catccccgc cctgatttt ctatttcca cgccatggg 
ggggcaaag ccccgaccc caaaaccag gcctttgtc 
gccgtccct cgccaccat tctggggct cctctaccc 
cggaatcat cctcatcga tcacggaca cttccagta 
taattgcat ctctctcgg actccttgt tctggcaac 
tagctgtct ccttctgga catgaactt gaccgctgc 
tatcttctt cactagtgc actcaacta caaacagat 
ggagctcta ccacgcgtt gccgttttt catgtacat 
gttgagctc ttgtgtccc aaagccggg ccaaagcct 
cacttccag tctgatcaa gttggcaca gatcgccat 
cactgtcat ggcggtgtt cgccatctt gtgggcgcc 
atttaatcg cgacttttt cgccgtctt gcgccgttt 
atttccgct ggatcgcgg cgttttcga ggacaaagt 
ctcgaattt ttggtgcgt ttttaacat tttctacaa 
attgaagct aaagtttgg taattacga aatgatgcg 
tatttgttt gtttgccac cgtctccat ggtgtttcc 
gtcaagtgt cgacttgtt tttgcgtcc gagtcgcaa 
aaagtttgt tttgtcgtt gattaattc ctcactggc 
gtttttctt gttcagtta tcaagtgca cgagaagag 
tattttact agttggcgt tcctgtggt gctgtattt 
cccccaaaa accgttcgt ttgtttctg gtttttgtg 
catttccgt ctttagcat gttgccgtt gttgtacaa 
agacggtct gattattgc aacaattgc gctaatgct 
tttttacgt catttcctt ccgtgtttc aattgaaca 
cttctatag gagttccct aaatgcgtc cgacggttt 
ggccgaata ttaccgaag tcttttgca cgtcttgct 
cgatattga gtacaaaca agcaacaca gatcgatat 
tttgaaact gacgtacaa acaaattgt aaaaaataa 
agaaactct gaaaaattt ggcgtttca tgttgtgtt 
agtgacgtc gttgattgg ttgtttggt gctttcgtt 
tgcactgtt agtttttga accgcctga gcgttatcc 
cgatttgtt cccactttt gatttcggc ttattcggc 
tttacattt tttcggatt ttttctgta tttcaatgt 
taaacaact acaaatacc gcaagagtt tgatgcgat 
taaaaatgt taaaatcaa gtcgtcttg aatgtaaat 
aaacgattg atttgtcg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY