Status
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Gene information
General information
TC015407 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 496881..498877
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015407
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05813

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late decreased - Number of animals showing the phenotype: 4

    egg chamber sometimes potentiallyfused - Number of animals showing the phenotype: 1

    vitellogenic egg chamber sometimes degenerated - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015407 RA
      MFCVKLSCE VWLVILGAL VAVLLRACT TLHPYSGAG 
      SPPMYGDYE AQRHWMEIT TNLRPLEWY KNTTDNDLM 
      YWGLDYPPL TAYHMYLTG KIGSFLNEN WTKLHESRG 
      FEGESHKIF MRYTVLAAD IVMYIPALI FYFHAMGGQ 
      SPDPKTRPL SPSLATILG LLYPGIILI DHGHFQYNC 
      ISLGLLVLA TSCLLLDMN LTAAIFFTS ALNYKQMEL 
      YHALPFFMY MLSSCVPKP GQSLTSSLI KLAQIAITV 
      MAVFAILWA PFNRDFFAV LRRLFPLDR GVFEDKVSN 
      FWCVFNIFY KLKLKFGNY EMMRICLFA TVSMVFPSS 
      VDLFLRPSR KKFVLSLIN SSLAFFLFS YQVHEKSIL 
      LVGVPVVLY FPQKPFVCF WFLCISVFS MLPLLYKDG 
      LIIATIALM LFYVISFRV SIEHFYRSS LNASDGLAE 
      YYRSLLHVL LDIEYKQAT QIDILKLTY KQIVKNKET 
      LKNLAFHVV LVTSLIGCL VLSFALLVF EPPERYPDL 
      FPLLISAYS ALHFFGFFL YFNVKQLQI PQEFDAIKN 
      VKIKSS
    CDS sequence
    • >TC015407 RA
      atgttttgc gtgaaatta agttgtgaa gtgtggtta 
      gtgattctg ggggccctt gtggccgtg ttactgcgc 
      gcgtgcact actctccac ccgtattcg ggcgccggc 
      agcccccca atgtacggc gattacgaa gcgcagcgc 
      cattggatg gaaatcacc acgaacttg cgccccctt 
      gagtggtac aagaacacc acagacaac gacctcatg 
      tactggggc ctcgactac ccccctctc acagcctac 
      cacatgtac ttaaccgga aaaatcggc agttttttg 
      aacgaaaac tggacgaaa ttgcacgag tccagaggc 
      tttgagggg gaatcccac aagattttc atgcgctat 
      accgtgcta gcggccgac atagtcatg tacatcccc 
      gccctgatt ttctatttc cacgccatg ggggggcaa 
      agccccgac cccaaaacc aggcctttg tcgccgtcc 
      ctcgccacc attctgggg ctcctctac cccggaatc 
      atcctcatc gatcacgga cacttccag tataattgc 
      atctctctc ggactcctt gttctggca actagctgt 
      ctccttctg gacatgaac ttgaccgct gctatcttc 
      ttcactagt gcactcaac tacaaacag atggagctc 
      taccacgcg ttgccgttt ttcatgtac atgttgagc 
      tcttgtgtc ccaaagccg ggccaaagc ctcacttcc 
      agtctgatc aagttggca cagatcgcc atcactgtc 
      atggcggtg ttcgccatc ttgtgggcg ccatttaat 
      cgcgacttt ttcgccgtc ttgcgccgt ttatttccg 
      ctggatcgc ggcgttttc gaggacaaa gtctcgaat 
      ttttggtgc gtttttaac attttctac aaattgaag 
      ctaaagttt ggtaattac gaaatgatg cgtatttgt 
      ttgtttgcc accgtctcc atggtgttt ccgtcaagt 
      gtcgacttg tttttgcgt ccgagtcgc aaaaagttt 
      gttttgtcg ttgattaat tcctcactg gcgtttttc 
      ttgttcagt tatcaagtg cacgagaag agtatttta 
      ctagttggc gttcctgtg gtgctgtat ttcccccaa 
      aaaccgttc gtttgtttc tggtttttg tgcatttcc 
      gtctttagc atgttgccg ttgttgtac aaagacggt 
      ctgattatt gcaacaatt gcgctaatg cttttttac 
      gtcatttcc ttccgtgtt tcaattgaa cacttctat 
      aggagttcc ctaaatgcg tccgacggt ttggccgaa 
      tattaccga agtcttttg cacgtcttg ctcgatatt 
      gagtacaaa caagcaaca cagatcgat attttgaaa 
      ctgacgtac aaacaaatt gtaaaaaat aaagaaact 
      ctgaaaaat ttggcgttt catgttgtg ttagtgacg 
      tcgttgatt ggttgtttg gtgctttcg tttgcactg 
      ttagttttt gaaccgcct gagcgttat cccgatttg 
      ttcccactt ttgatttcg gcttattcg gctttacat 
      tttttcgga ttttttctg tatttcaat gttaaacaa 
      ctacaaata ccgcaagag tttgatgcg attaaaaat 
      gttaaaatc aagtcgtct tga
    mRNA sequence
    • >TC015407 RA
      aatcgtaac aaaaaaaaa ttcaaaaaa cttcttgat 
      ttgcacaac tgcaccaaa caaacaatt tcgcaactc 
      ccaacccta aaacccacc aaattcacc cccatttca 
      tgccaccac cactccgtc tgtcatctg ccaaaaaac 
      cccccatca acggaatat cccgtttgt tggcccctt 
      tctccctga aaatgcctc aatacacca gcgaccaca 
      tccgcacca aactcctcc aagaactgt ccgcctcac 
      acgtggtaa gtttcccaa ctttaattt ttttaataa 
      caaaactgt caaaaaaaa ccctaacct cacaatcac 
      caccaccat gttttgcgt gaaattaag ttgtgaagt 
      gtggttagt gattctggg ggcccttgt ggccgtgtt 
      actgcgcgc gtgcactac tctccaccc gtattcggg 
      cgccggcag ccccccaat gtacggcga ttacgaagc 
      gcagcgcca ttggatgga aatcaccac gaacttgcg 
      cccccttga gtggtacaa gaacaccac agacaacga 
      cctcatgta ctggggcct cgactaccc ccctctcac 
      agcctacca catgtactt aaccggaaa aatcggcag 
      ttttttgaa cgaaaactg gacgaaatt gcacgagtc 
      cagaggctt tgaggggga atcccacaa gattttcat 
      gcgctatac cgtgctagc ggccgacat agtcatgta 
      catccccgc cctgatttt ctatttcca cgccatggg 
      ggggcaaag ccccgaccc caaaaccag gcctttgtc 
      gccgtccct cgccaccat tctggggct cctctaccc 
      cggaatcat cctcatcga tcacggaca cttccagta 
      taattgcat ctctctcgg actccttgt tctggcaac 
      tagctgtct ccttctgga catgaactt gaccgctgc 
      tatcttctt cactagtgc actcaacta caaacagat 
      ggagctcta ccacgcgtt gccgttttt catgtacat 
      gttgagctc ttgtgtccc aaagccggg ccaaagcct 
      cacttccag tctgatcaa gttggcaca gatcgccat 
      cactgtcat ggcggtgtt cgccatctt gtgggcgcc 
      atttaatcg cgacttttt cgccgtctt gcgccgttt 
      atttccgct ggatcgcgg cgttttcga ggacaaagt 
      ctcgaattt ttggtgcgt ttttaacat tttctacaa 
      attgaagct aaagtttgg taattacga aatgatgcg 
      tatttgttt gtttgccac cgtctccat ggtgtttcc 
      gtcaagtgt cgacttgtt tttgcgtcc gagtcgcaa 
      aaagtttgt tttgtcgtt gattaattc ctcactggc 
      gtttttctt gttcagtta tcaagtgca cgagaagag 
      tattttact agttggcgt tcctgtggt gctgtattt 
      cccccaaaa accgttcgt ttgtttctg gtttttgtg 
      catttccgt ctttagcat gttgccgtt gttgtacaa 
      agacggtct gattattgc aacaattgc gctaatgct 
      tttttacgt catttcctt ccgtgtttc aattgaaca 
      cttctatag gagttccct aaatgcgtc cgacggttt 
      ggccgaata ttaccgaag tcttttgca cgtcttgct 
      cgatattga gtacaaaca agcaacaca gatcgatat 
      tttgaaact gacgtacaa acaaattgt aaaaaataa 
      agaaactct gaaaaattt ggcgtttca tgttgtgtt 
      agtgacgtc gttgattgg ttgtttggt gctttcgtt 
      tgcactgtt agtttttga accgcctga gcgttatcc 
      cgatttgtt cccactttt gatttcggc ttattcggc 
      tttacattt tttcggatt ttttctgta tttcaatgt 
      taaacaact acaaatacc gcaagagtt tgatgcgat 
      taaaaatgt taaaatcaa gtcgtcttg aatgtaaat 
      aaacgattg atttgtcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY