Status
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Gene information
General information
TC015405 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 442708..452354
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015405
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05812

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Many background defects. None of them is really penetrant.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015405 RA
      MDPENKPKT NEKLDSTLK KFLSAQPFD SDDITESAK 
      QIFQELNQN YDHFKLVTI SDDLDEILP KKRDPKTPI 
      YAILEKLKQ RWVVLCLWG DKAYYKDSS GEKIPHRIE 
      KKFDTLTIF ENFDQDHDA DAVENYGPF SLRNLQILL 
      KLGQEIGEK FPSQKFCQL ADVDEEKVN IIVKNGQEF 
      AQKNDFKNA LRQFIETLP LDLGARMLK YKQILEEEL 
      DKDETLVDH ERIKLARLE YLTDENVTK LQENYKVND 
      SSVEIEVVK QFPEEMDKL GQIFATLDH ITDQKLREN 
      ALEQLAILL NLDANKLKS FYASKGPKT PLKKPQLTL 
      DDISKIVDN LPKLDKTTK DAETTPFDQ LLDQVTLGQ 
      DLTPSSPAQ FTPNKSQLE QNYYQVKEK YQLWRGSTL 
      HDINTWARS QKGKPGNFC EIVAVCDRV FALLTGGHR 
      LRDTQILAV LLFNDVEKR GRLCQVQTG EGKTTIVSI 
      LAVVGALQG HLIDVVTTS PVLASDGVD KNRDFYAVF 
      GLTVGTTDT PGGYTADVL YGSIASFQF DYLRESFEG 
      LNVRNDRRF GLVILDEVD SLLVDNGGH IAKIATPYP 
      GMESLRYVF IKIWAELAK AEERFIEEN QSKLESIVT 
      NDSAEQLFD EFWAQDERQ VIRDLIKSA MPATSPLIP 
      QHLSHYVAR NLDDWIESA LVAKYDCHE LEQYRIVNG 
      VVTPVDNLN TGVTLTNTV WSKGLQQFL QLKHNLHLT 
      CSTLSSIFI SNFGYIQKY AKIFGVTGT LGSTTEQDL 
      LSHVYNVTF AKIPTHKPK SFTEEPPIV TKDNSWDLE 
      VTSEIMRKI DEKRAVLVI FATERDLNT VGRNLEIIR 
      HDDFEIRYI RHEGEVKDA SLVVKASSV VLATNLAGR 
      GCDFKTDQS VEERGGLHV IVAFLPANQ RVEDQAFGR 
      TSRNGNRGS GQLIVRESE ASGGGLKQT RDLQERGRL 
      KHVRDVLIK EIVFKEDLI GRFADLYLK LKRAKVLEG 
      EFCYFLQDL KEFWAFWLD SRNFHMITQ SVDEVFGEF 
      LGEAEYIIK GEVKHNPFY SIALADHYL ENNKKAKAR 
      SELLKVVSS GANHTELLA GAHLKMFEL AIASGNQTW 
      HKIRKALAK VLFIDVQKN TLYKKNALK QLGHANAAI 
      TTEIDFIES WLQSPDFER ILPKDNFLK QVNSRLYCL 
      KIQKGNIEE LVKQIEEAG DDGVDFGGK AQSTVGNYR 
      KMDKSSKEI VGETELSEL QAVGIDALY TLRTVKDVP 
      DYVIHAAQG QIGAGIAAL AAGLFFPPI FPIMSPLGV 
      TLISEGIID IVFALFEDN PEFKDFMKG KAVSYGIAL 
      VTFGLSAIA QSIKIITKA LSFFRKLAT FLRKSSYLK 
      GLSAKLAKV VDKLANKME RLLDVAKFN KLTRSQQLS 
      QLKELQKSG KAFDHLGGL SKLQSLEKL QKLGKLKEL 
      TRSQLLVQF AKEVAWKTA EEGGKRLLE KFVIKPFLG 
      RLYGDVIEK IKAFLVKSL KQSIQDNAN LREKLRTTD 
      KKQIDETLE RFIKEDVVI EKLRDYLFD SLGKFKSTK 
      FHVLIHGVK MAVNVGKMV HICTKFAAN LEKQLKGGD 
      ADNNVDKII DKICTKLVD EVLTICWDV LKDNVKFVW 
      KHKDVFASK PTDLVLKPA EKTGNSWKV FGLEPGASL 
      DEVKTHYRK MALVLHPDK NLNDPTATE KFQEFQNAY 
      EDILKTF
    CDS sequence
    • >TC015405 RA
      atggatcca gaaaacaag ccaaaaacc aacgaaaaa 
      cttgactca actctcaaa aaattcctc tcggctcaa 
      cctttcgac tcagacgac atcaccgaa tccgccaaa 
      caaatcttc caagaattg aaccaaaac tacgaccac 
      ttcaaacta gtgacaatc tccgacgat ttggacgaa 
      attctccca aaaaaacgc gaccccaaa accccaatt 
      tacgcaatt ttggaaaaa ttgaaacaa cgatgggtc 
      gttttgtgc ctttggggg gacaaagcc tactacaag 
      gactcatca ggcgaaaaa attccccat cgaattgaa 
      aaaaaattc gacactttg acaattttc gaaaatttt 
      gaccaagac cacgacgct gacgcggta gagaattac 
      gggcctttc agtcttcgg aatttacaa attttgcta 
      aagttgggc caagaaatc ggcgaaaaa ttcccaagc 
      caaaaattt tgccaattg gctgatgtt gacgaagag 
      aaagtcaat ataattgtc aaaaatggg caagaattt 
      gctcaaaaa aatgatttt aaaaacgcc ctacgacag 
      tttattgag acactaccg ctagatttg ggggcacgt 
      atgctaaag tacaagcaa atccttgag gaagagctg 
      gataaggac gaaactttg gttgaccac gagcggatc 
      aagttggct cgtctggag tacctaacc gacgaaaat 
      gtcactaaa ttgcaagaa aattacaaa gtcaatgat 
      tcaagtgtt gaaatcgaa gtcgtcaaa caatttccg 
      gaggaaatg gacaagttg ggccaaatt tttgcaact 
      cttgaccac atcactgat cagaaactg cgtgaaaac 
      gcattagaa cagctcgcg attttgcta aatttagac 
      gccaacaag ctcaagtca ttttatgcg tccaaaggc 
      ccgaaaacg ccactcaaa aaaccgcaa ctaaccctt 
      gacgatatc agcaaaatt gtcgacaat ttgccaaaa 
      cttgacaaa acgaccaag gacgcggaa accaccccc 
      tttgaccaa cttttggat caagtgacc cttggccaa 
      gacctgacc ccttcatcc cccgcccag ttcaccccc 
      aacaagagt caactcgaa caaaactac taccaggtc 
      aaggaaaaa taccaactt tggcgaggt agcacttta 
      cacgacatt aacacttgg gctaggagt caaaagggc 
      aaacctggc aatttttgc gaaattgtg gcggtttgt 
      gaccgcgtt ttcgccctt ttgacaggt gggcaccgc 
      ctacgtgat acccagatt ttggccgtt ttgctcttc 
      aatgacgtt gaaaaacgc ggtcgcctg tgccaggtc 
      caaacaggt gaaggcaaa acaacaata gtgtcaatt 
      ctggcagtt gtgggggcc ttgcagggc cacttgatt 
      gacgtggtc accactagt ccagtgttg gcaagtgat 
      ggtgttgac aaaaatcgg gatttttac gccgttttt 
      gggctcacg gttggcacc actgacacg ccgggtggc 
      tacactgcc gatgtgctg tacgggagt attgccagt 
      ttccagttt gattatttg agggaaagt tttgagggg 
      ttaaatgtt cgaaatgat cggcggttt ggtctagtt 
      atcttggac gaggttgat agtcttttg gttgacaat 
      ggcggacat attgccaaa attgcaacc ccgtacccg 
      ggaatggag agtctaagg tacgttttt atcaaaatt 
      tgggctgag ttggccaag gctgaggaa cgttttatt 
      gaggaaaac cagtcaaaa ctagagtca attgtgaca 
      aatgacagt gcggagcaa ttgtttgac gagttttgg 
      gcgcaagac gaacgccaa gtcattcgg gacctaatc 
      aagagtgcc atgccggca accagccca ctaatcccc 
      cagcacttg tcacactat gttgcgcgc aatttggac 
      gattggatc gagagtgcc ttggtcgcc aagtacgat 
      tgtcacgag ttggaacaa tatcgcatc gtcaacggt 
      gttgtgact cctgttgac aacctaaac accggcgtg 
      accttgact aatacagtg tggtccaaa ggactccaa 
      cagttcctc caattgaag cacaatctt catctgacc 
      tgttctacc ctaagtagc atctttatt tccaacttt 
      ggttacatc cagaagtat gccaaaatt tttggggtc 
      actggaacc ttgggctcc accaccgag caagacctg 
      ctatcccac gtctataat gtcactttt gccaaaata 
      ccaacccac aaacccaaa agcttcacc gaagaaccg 
      ccaattgtc acaaaagac aatagttgg gatttggaa 
      gtaacgtcc gaaatcatg cgaaaaatt gacgaaaag 
      cgggccgtt ttggtgatt tttgccacc gaacgtgac 
      cttaacacc gttggacgt aaccttgaa atcatccgc 
      cacgatgac ttcgaaatc cgctacatt cggcacgaa 
      ggtgaagtc aaggacgcc agcctagta gtaaaggcc 
      tctagcgtt gtcttggct acgaacctc gcggggcgt 
      ggctgcgac ttcaaaacg gaccaatcg gtggaggag 
      aggggcggt ttgcacgtc attgtggcc ttcctaccg 
      gccaatcag cgtgtggaa gaccaggcc ttcgggcgg 
      acgtcacgc aacggaaat aggggaagt gggcaattg 
      atcgtgcgc gagagtgag gcgagtggt gggggtttg 
      aaacaaaca cgtgatcta caagagagg gggcgacta 
      aaacacgtt cgtgacgtt ttaatcaaa gaaatcgtc 
      ttcaaggag gacttgatc gggcgtttt gctgacttg 
      tacctgaaa ctgaaacgt gccaaagtg ctggaaggg 
      gagttttgc tactttttg caggatttg aaggagttt 
      tgggccttt tggttggat tcccgcaac ttccacatg 
      atcacacaa agtgtggac gaagttttt ggcgagttt 
      ttgggcgaa gctgagtat attattaag ggagaagtt 
      aaacataat cccttttat tccatcgca ttggctgac 
      cattactta gaaaacaat aaaaaagct aaggctaga 
      agtgagttg ctcaaggtg gtgtcaagt ggtgccaac 
      catacggaa ttgttggct ggggcccac ttgaaaatg 
      ttcgaactg gcgatagct agtgggaac cagacgtgg 
      cacaagatc aggaaggcg ttggctaag gttttgttt 
      attgacgtt cagaagaac acactgtat aagaaaaac 
      gccttaaaa cagttgggg cacgctaac gcggccata 
      actaccgag attgatttt atcgaaagt tggctacaa 
      agtcctgat tttgagcga atccttcca aaggataat 
      tttctaaaa caagtgaat tcaaggttg tattgtttg 
      aaaattcag aaagggaat attgaggag ttggtgaaa 
      cagatcgaa gaggctggg gacgacggg gttgatttt 
      ggggggaag gcgcaatcg acggttggg aattatcga 
      aagatggac aaaagttct aaagaaatc gtaggtgag 
      acagagttg agtgaactc caagccgtt ggtatcgac 
      gctctttac acccttcga actgtcaaa gacgtccct 
      gattacgtc atccatgcg gctcaaggt caaatcggt 
      gcaggaatt gcagctttg gccgcagga ctttttttc 
      ccgcccatt tttccaatc atgagccca cttggtgta 
      accctaatt tccgaaggg ataatagac attgttttc 
      gcccttttc gaggacaat cccgagttc aaggacttt 
      atgaagggc aaagcggtg tcatacggc attgccttg 
      gttactttc ggcctcagc gccatcgcc caatcaatc 
      aaaatcata accaaagct ttgagcttt ttccgaaag 
      ttggcaact ttcctacgc aaaagctcc tacttgaag 
      ggcttaagt gccaagttg gccaaagtt gtggacaag 
      ttagcgaac aaaatggag cgcctacta gatgtcgcc 
      aaatttaac aaactgaca aggagtcaa caactaagc 
      caactgaaa gagttgcaa aaaagcggc aaagctttc 
      gaccatttg ggcggttta tccaagtta cagagtttg 
      gaaaagttg caaaaactg ggcaaattg aaggaactg 
      acccgcagc caactcctg gtgcagttc gccaaagag 
      gtggcgtgg aaaacggca gaagaaggc ggcaaacgt 
      ttattggaa aaatttgtt attaaaccg tttctgggc 
      cgactttac ggcgacgtg atcgaaaaa atcaaggcg 
      tttctggtc aaaagtctg aaacaatcg attcaagac 
      aacgccaac ttgcgcgaa aaactccga acgactgat 
      aaaaaacaa attgacgaa actttggag cgctttatt 
      aaagaagac gtcgttatt gaaaaattg cgtgactat 
      ctttttgac agtttggga aaattcaaa agtacaaag 
      tttcacgtt ttgatccac ggagtcaag atggccgtg 
      aatgtgggc aagatggtt cacatctgt acgaaattt 
      gcggcaaat cttgaaaaa caactgaaa ggtggggac 
      gctgacaac aacgttgat aagataatt gataaaatt 
      tgcacaaag ttggtcgat gaggtcttg acgatttgt 
      tgggacgtc ctgaaggat aatgtcaaa tttgtatgg 
      aaacataag gacgttttt gcaagtaaa ccgactgat 
      cttgtcctg aaaccggct gaaaaaact ggaaattcc 
      tggaaagtt tttgggctt gaaccagga gcgtctctt 
      gatgaggtt aagacgcat tacaggaag atggcgttg 
      gttttgcac cccgataag aatttgaac gatccgacg 
      gccactgaa aagtttcag gagtttcag aatgcttac 
      gaagacatt ctgaagaca ttttga
    mRNA sequence
    • >TC015405 RA
      caggtagta aaatctaga ggcacgtgt gagtgcaaa 
      agttaattt tttcactga cctgacagt agaaaagcc 
      ttggctttg tttcccgat taataattc attgatttt 
      catagcatt tttgcggta actgcactg agcgcacct 
      ttgccacca actgatgaa agacataaa gcccaagag 
      gatatttat agttcggac gccatttcc gagcgctta 
      tttcgacac cgtgtaaac gaactttaa ttattactc 
      ggcggaatg aaatttttt cgtttaaat tgtaaaaaa 
      tggatccag aaaacaagc caaaaacca acgaaaaac 
      ttgactcaa ctctcaaaa aattcctct cggctcaac 
      ctttcgact cagacgaca tcaccgaat ccgccaaac 
      aaatcttcc aagaattga accaaaact acgaccact 
      tcaaactag tgacaatct ccgacgatt tggacgaaa 
      ttctcccaa aaaaacgcg accccaaaa ccccaattt 
      acgcaattt tggaaaaat tgaaacaac gatgggtcg 
      ttttgtgcc tttgggggg acaaagcct actacaagg 
      actcatcag gcgaaaaaa ttccccatc gaattgaaa 
      aaaaattcg acactttga caattttcg aaaattttg 
      accaagacc acgacgctg acgcggtag agaattacg 
      ggcctttca gtcttcgga atttacaaa ttttgctaa 
      agttgggcc aagaaatcg gcgaaaaat tcccaagcc 
      aaaaatttt gccaattgg ctgatgttg acgaagaga 
      aagtcaata taattgtca aaaatgggc aagaatttg 
      ctcaaaaaa atgatttta aaaacgccc tacgacagt 
      ttattgaga cactaccgc tagatttgg gggcacgta 
      tgctaaagt acaagcaaa tccttgagg aagagctgg 
      ataaggacg aaactttgg ttgaccacg agcggatca 
      agttggctc gtctggagt acctaaccg acgaaaatg 
      tcactaaat tgcaagaaa attacaaag tcaatgatt 
      caagtgttg aaatcgaag tcgtcaaac aatttccgg 
      aggaaatgg acaagttgg gccaaattt ttgcaactc 
      ttgaccaca tcactgatc agaaactgc gtgaaaacg 
      cattagaac agctcgcga ttttgctaa atttagacg 
      ccaacaagc tcaagtcat tttatgcgt ccaaaggcc 
      cgaaaacgc cactcaaaa aaccgcaac taacccttg 
      acgatatca gcaaaattg tcgacaatt tgccaaaac 
      ttgacaaaa cgaccaagg acgcggaaa ccaccccct 
      ttgaccaac ttttggatc aagtgaccc ttggccaag 
      acctgaccc cttcatccc ccgcccagt tcaccccca 
      acaagagtc aactcgaac aaaactact accaggtca 
      aggaaaaat accaacttt ggcgaggta gcactttac 
      acgacatta acacttggg ctaggagtc aaaagggca 
      aacctggca atttttgcg aaattgtgg cggtttgtg 
      accgcgttt tcgcccttt tgacaggtg ggcaccgcc 
      tacgtgata cccagattt tggccgttt tgctcttca 
      atgacgttg aaaaacgcg gtcgcctgt gccaggtcc 
      aaacaggtg aaggcaaaa caacaatag tgtcaattc 
      tggcagttg tgggggcct tgcagggcc acttgattg 
      acgtggtca ccactagtc cagtgttgg caagtgatg 
      gtgttgaca aaaatcggg atttttacg ccgtttttg 
      ggctcacgg ttggcacca ctgacacgc cgggtggct 
      acactgccg atgtgctgt acgggagta ttgccagtt 
      tccagtttg attatttga gggaaagtt ttgaggggt 
      taaatgttc gaaatgatc ggcggtttg gtctagtta 
      tcttggacg aggttgata gtcttttgg ttgacaatg 
      gcggacata ttgccaaaa ttgcaaccc cgtacccgg 
      gaatggaga gtctaaggt acgttttta tcaaaattt 
      gggctgagt tggccaagg ctgaggaac gttttattg 
      aggaaaacc agtcaaaac tagagtcaa ttgtgacaa 
      atgacagtg cggagcaat tgtttgacg agttttggg 
      cgcaagacg aacgccaag tcattcggg acctaatca 
      agagtgcca tgccggcaa ccagcccac taatccccc 
      agcacttgt cacactatg ttgcgcgca atttggacg 
      attggatcg agagtgcct tggtcgcca agtacgatt 
      gtcacgagt tggaacaat atcgcatcg tcaacggtg 
      ttgtgactc ctgttgaca acctaaaca ccggcgtga 
      ccttgacta atacagtgt ggtccaaag gactccaac 
      agttcctcc aattgaagc acaatcttc atctgacct 
      gttctaccc taagtagca tctttattt ccaactttg 
      gttacatcc agaagtatg ccaaaattt ttggggtca 
      ctggaacct tgggctcca ccaccgagc aagacctgc 
      tatcccacg tctataatg tcacttttg ccaaaatac 
      caacccaca aacccaaaa gcttcaccg aagaaccgc 
      caattgtca caaaagaca atagttggg atttggaag 
      taacgtccg aaatcatgc gaaaaattg acgaaaagc 
      gggccgttt tggtgattt ttgccaccg aacgtgacc 
      ttaacaccg ttggacgta accttgaaa tcatccgcc 
      acgatgact tcgaaatcc gctacattc ggcacgaag 
      gtgaagtca aggacgcca gcctagtag taaaggcct 
      ctagcgttg tcttggcta cgaacctcg cggggcgtg 
      gctgcgact tcaaaacgg accaatcgg tggaggaga 
      ggggcggtt tgcacgtca ttgtggcct tcctaccgg 
      ccaatcagc gtgtggaag accaggcct tcgggcgga 
      cgtcacgca acggaaata ggggaagtg ggcaattga 
      tcgtgcgcg agagtgagg cgagtggtg ggggtttga 
      aacaaacac gtgatctac aagagaggg ggcgactaa 
      aacacgttc gtgacgttt taatcaaag aaatcgtct 
      tcaaggagg acttgatcg ggcgttttg ctgacttgt 
      acctgaaac tgaaacgtg ccaaagtgc tggaagggg 
      agttttgct actttttgc aggatttga aggagtttt 
      gggcctttt ggttggatt cccgcaact tccacatga 
      tcacacaaa gtgtggacg aagtttttg gcgagtttt 
      tgggcgaag ctgagtata ttattaagg gagaagtta 
      aacataatc ccttttatt ccatcgcat tggctgacc 
      attacttag aaaacaata aaaaagcta aggctagaa 
      gtgagttgc tcaaggtgg tgtcaagtg gtgccaacc 
      atacggaat tgttggctg gggcccact tgaaaatgt 
      tcgaactgg cgatagcta gtgggaacc agacgtggc 
      acaagatca ggaaggcgt tggctaagg ttttgttta 
      ttgacgttc agaagaaca cactgtata agaaaaacg 
      ccttaaaac agttggggc acgctaacg cggccataa 
      ctaccgaga ttgatttta tcgaaagtt ggctacaaa 
      gtcctgatt ttgagcgaa tccttccaa aggataatt 
      ttctaaaac aagtgaatt caaggttgt attgtttga 
      aaattcaga aagggaata ttgaggagt tggtgaaac 
      agatcgaag aggctgggg acgacgggg ttgattttg 
      gggggaagg cgcaatcga cggttggga attatcgaa 
      agatggaca aaagttcta aagaaatcg taggtgaga 
      cagagttga gtgaactcc aagccgttg gtatcgacg 
      ctctttaca cccttcgaa ctgtcaaag acgtccctg 
      attacgtca tccatgcgg ctcaaggtc aaatcggtg 
      caggaattg cagctttgg ccgcaggac tttttttcc 
      cgcccattt ttccaatca tgagcccac ttggtgtaa 
      ccctaattt ccgaaggga taatagaca ttgttttcg 
      cccttttcg aggacaatc ccgagttca aggacttta 
      tgaagggca aagcggtgt catacggca ttgccttgg 
      ttactttcg gcctcagcg ccatcgccc aatcaatca 
      aaatcataa ccaaagctt tgagctttt tccgaaagt 
      tggcaactt tcctacgca aaagctcct acttgaagg 
      gcttaagtg ccaagttgg ccaaagttg tggacaagt 
      tagcgaaca aaatggagc gcctactag atgtcgcca 
      aatttaaca aactgacaa ggagtcaac aactaagcc 
      aactgaaag agttgcaaa aaagcggca aagctttcg 
      accatttgg gcggtttat ccaagttac agagtttgg 
      aaaagttgc aaaaactgg gcaaattga aggaactga 
      cccgcagcc aactcctgg tgcagttcg ccaaagagg 
      tggcgtgga aaacggcag aagaaggcg gcaaacgtt 
      tattggaaa aatttgtta ttaaaccgt ttctgggcc 
      gactttacg gcgacgtga tcgaaaaaa tcaaggcgt 
      ttctggtca aaagtctga aacaatcga ttcaagaca 
      acgccaact tgcgcgaaa aactccgaa cgactgata 
      aaaaacaaa ttgacgaaa ctttggagc gctttatta 
      aagaagacg tcgttattg aaaaattgc gtgactatc 
      tttttgaca gtttgggaa aattcaaaa gtacaaagt 
      ttcacgttt tgatccacg gagtcaaga tggccgtga 
      atgtgggca agatggttc acatctgta cgaaatttg 
      cggcaaatc ttgaaaaac aactgaaag gtggggacg 
      ctgacaaca acgttgata agataattg ataaaattt 
      gcacaaagt tggtcgatg aggtcttga cgatttgtt 
      gggacgtcc tgaaggata atgtcaaat ttgtatgga 
      aacataagg acgtttttg caagtaaac cgactgatc 
      ttgtcctga aaccggctg aaaaaactg gaaattcct 
      ggaaagttt ttgggcttg aaccaggag cgtctcttg 
      atgaggtta agacgcatt acaggaaga tggcgttgg 
      ttttgcacc ccgataaga atttgaacg atccgacgg 
      ccactgaaa agtttcagg agtttcaga atgcttacg 
      aagacattc tgaagacat tttgatttg tgggaaaat 
      agttttttt tggtgattt caacaagcg acgaaccct 
      gtaatttac gtaatcaat aaagtgttc aagtcagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY