Status
Please wait ...
Gene information
General information
TC015399 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 351051..364796
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015399
    iBeetle screen iB_05811

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015399 RA
      MDSNTTNFQ FGQWLATPP PGEEVVVTG MSGRFPASN 
      NVQEFRDNL FAKKYMMDK IDKRWKVVN KEIPSKGGL 
      LPEIGTFDP GFFGIHHRQ AENMDPAMR NFLEVAVEA 
      ILDSGFHPK ELEGSRTGV FVGYSWSDL EAPSLSEAD 
      EPQKFGMTG YSRSFIPHR ISYYLKLKG PSVVVDSAC 
      SSSFNGLEH AFKAIRTGK CDNALVGGC NLTLHPGTT 
      LQFARYGLL SKDCICKVF DQEANGYVR SETIACVFL 
      QKAKNSRRI YAQIVGAKI NDDGFKSEG LSFPSHKMQ 
      GQLLKEIYN ECGIVPSRI TYMELHGTG TKVGDGEEL 
      QAVDDAIVT QREKTLIIG SVKSNIGHA EPASGMSSL 
      IKVLIAMEE GKIAPNMNL NQIKAGFTS LEQGKLRVA 
      TEITPLEGE EPIVGISNF GFGGNNCHV ILKRVSKKN 
      LKYHDEVPR LVCVSGRTV QAVEEILNK VKSKFNVEF 
      IAMLHQLFK NRFTTHLYR GYALVSKNG TFGTSISQI 
      KNESNECRV HFGNFQKCY LEVCQYFML FPKFRSTME 
      RINEILGGG TTLYDTLSS TKTTLLASV CTQFSILET 
      LRDLGVVPK LDSTTNLAH LIQTLPLEQ LLETVRNID 
      SENHKFQVL TASTRTQIL EPEPENDQT FVLDVTKGL 
      VAFLDGLGR LYQNGVDLH LEKLYAPVE FPVSRATPM 
      ISPLVKWYH DKIWHSTAF TAFAKKTDA KYDIGLMDE 
      NSYLEGHVI DGRNLFPAT GYLNIIWEI IAKERYAQP 
      IHLPIVFEN CKFVRATTM PKAGFITFA VSIQRKSGF 
      FEIVENDSI VMTGRVLVP DDIKSYQVD LPELKVSDD 
      PKEYLDEFE VYKELYLRG YNYSGLFKG LVKCNIDGS 
      SGLVRWDNN WISFMDKML QIKILELDS RLLYVPTGI 
      KKLSIDPIK HLEIVEKKS DHLIPVSSY KNSDVIRCG 
      GIEILGLQA NAISKRKPI LEPVLEKSI FIPNATDLD 
      LTQAIRVNT QIILDESLE KNFTALEIL DEFSNPEVS 
      FVMPLLFNA LEDIPLIHP KLTISTKTK IDDIPGVKI 
      EATTLSSTS NLLLIVATK LFQRVTLLN QVLSALSQN 
      GFALSREDA NFEPEFGDK ITILTQFVT PDEKLILFK 
      KTESGKFNQ KLINVSSEN FNWVQHVKE ELKTDTRVV 
      LYSEKGALE GLLGLVNCL RKEPGGTKI SCVSIMGQE 
      IPQLDEQLA KNLAFNVYK NQKWGTYRH LHLDEAVQS 
      RKDHAFVSL LSRGDLSSL RWVEGPLSK TDPKVIFAN 
      YCAINFKDI MTATGRIVL AMERTKQDS IEGLEYSGR 
      DFNGTRIMG MTDHRAMGT LINPDLPLC WRIPDHWSL 
      EDAATVPVV YSTVIYGLL MRSTLAPGT SILIHSGTG 
      GIGLAALNV ALAHGCDVY VTVGTKEKR EFLRKNFPQ 
      ISDDHIGNS RDLSFEEMI KTQTKGRGV DIVLNSLTE 
      EKLRASVRC LARGGKFFE IGKFDLVNN NAIQLLLLE 
      KGASYHGIM VDTLFKESD EDKLKLNRK VEEGIRKGY 
      VKPIPRTVF ESDNVEAAF RYMMTGKHI GKVLVKIRE 
      ENQADCSIQ CLPKFWADP SKSYIILGG LGGFGLELA 
      DWLVLRGAR NLVLTSRTG VQTGYQTQR IKIWQSYGA 
      RVNISTITI SSKDDCVKL IQEATKLAP VDAIFNLAV 
      VLKDELFEN QTEENFRIS LTPKAFATK YLDQVSRDL 
      CPNLRFFVV FSSVSCGRG NIGQSNYGM ANSIMERVC 
      EKRKSEGFP ALAIQWGAI GDVGLVAKM QKDNKELVI 
      GGTLQQKIS SCLNVLDRL LNQDEPIVS SMVVAEKRD 
      KRIGGESAV DVVAALLGI KDIKTVSQQ SSLSELGMD 
      SMMGNEVMQ VLEKEFEIY VTPKDLRTM TFAKLVELE 
      AQRTSNIQD QKSKQKMEQ GTNTIIQFI PDGQFDNQE 
      LIKLERVEG VFKQLEPFV KNLNARVFG VQYSYQQPE 
      HTIQEVVEN ILPKIEKHI KTEKKFFFI GYSFGVTVA 
      LELALRLEA ASYTGTVVA IDGSPHYTR RLLEHYLPG 
      ETAAEFESM ILVNILAIF LPLDLIVKN KEKLMSCPD 
      FDARIETVR SFVPSDLPN LQKMDKQVA FTLRERFRA 
      ILDYKSNGN KLKSTTIKL YKAEHPPVA LENMDEDYK 
      LAEFSESKV EAMTICGNH ISMLEQKEL VNDVSQIFA 
      LDFY
    CDS sequence
    • >TC015399 RA
      atggactca aacaccacc aacttccaa ttcggacaa 
      tggctggca actccaccg cctggagag gaagtcgtc 
      gttacaggg atgtcgggg cgattcccg gcctccaac 
      aacgtccag gaatttcgg gacaatctc ttcgccaag 
      aaatacatg atggacaaa atcgacaaa cgctggaag 
      gtcgtaaac aaggagatt ccttcaaaa ggtggcctt 
      cttcccgaa atcgggact tttgatccg ggatttttc 
      gggattcat catcgacaa gccgaaaac atggaccct 
      gcgatgcgc aatttcctg gaagttgct gtggaggcg 
      attctggat tcgggattc caccccaag gagttggag 
      gggagcagg acgggggtt tttgtgggg tactcctgg 
      tcggacctg gaggcgccc agtttgagc gaagccgac 
      gagccccaa aagttcggg atgacgggc tacagccgc 
      agtttcatt ccacatcgc atcagctac tacttgaag 
      ctcaaaggg ccttccgtt gtggtggac tcggcttgt 
      agcagctcg ttcaatggc cttgagcat gccttcaag 
      gccataagg acgggcaag tgtgataat gcccttgtg 
      ggcggttgt aacctaacc cttcatcct ggtaccacc 
      ctacagttt gcaaggtac ggccttttg agcaaagat 
      tgcatttgc aaagtgttt gatcaagag gctaatgga 
      tatgtgcga agtgagaca attgcttgt gtttttctg 
      caaaaggca aaaaattca cgcaggatt tatgcccaa 
      atcgtgggt gcaaaaatc aacgacgac ggtttcaaa 
      agcgaaggc ctatctttt ccctctcac aaaatgcaa 
      ggccaactc ctcaaagaa atctacaac gaatgtgga 
      atagtacca tccagaata acctacatg gaactacat 
      ggtactggt accaaagtg ggcgatggg gaagagctt 
      caagctgta gacgacgcc attgtgacc caacgtgaa 
      aaaacccta attataggt tccgtcaag tcaaacata 
      ggccatgcg gaaccagct tctggtatg tcctccctt 
      atcaaggta ctgatagct atggaagag ggaaaaatt 
      gcaccaaac atgaacttg aaccaaatt aaggccggg 
      ttcacatcc cttgagcag ggaaaatta agggtggcc 
      acggaaatc acccccttg gagggggaa gaacccatt 
      gttgggatt agcaatttt ggattcggt ggtaacaat 
      tgtcacgtg attttgaaa cgcgtgtcc aaaaagaac 
      ctgaagtac catgacgaa gtaccacga ctggtttgt 
      gtgagtggt cgtactgtt caagcagtt gaagaaata 
      cttaataag gttaaatca aaattcaac gttgaattt 
      atcgcaatg ttgcaccaa ctcttcaaa aatcgcttt 
      accacgcat ttgtaccgt gggtacgcc ctagtgtca 
      aaaaatggt acttttggt acttcaatt agccaaata 
      aagaacgaa tcaaacgag tgccgggtg catttcggc 
      aactttcaa aagtgttac cttgaagtt tgtcagtac 
      tttatgtta ttcccaaaa tttcgaagt accatggaa 
      cgtataaat gaaattttg ggcggtggt accacctta 
      tacgacact ctttcgagt accaaaacc accctactt 
      gcaagcgtg tgcacacaa ttctcaatc cttgaaact 
      ttgcgtgac cttggagtg gtaccaaaa ctggacagt 
      accaccaac ttggctcac ttgatccaa acattacca 
      ctggaacag ttactcgaa acggtgcga aacattgac 
      tcggaaaac cacaaattt caagttttg actgcaagt 
      accagaacc caaattttg gaacccgaa cccgaaaat 
      gaccaaact tttgtgttg gacgtgaca aaaggtttg 
      gttgcgttc ttagacggg cttggacgg ttgtaccaa 
      aatggagtt gatttgcat ttagaaaag ttgtatgca 
      ccggttgaa tttccggtc agtcgggcc acgcccatg 
      atatcgcca ttggtcaag tggtaccat gacaagatt 
      tggcacagt actgctttt acagcattt gcgaaaaaa 
      accgacgcc aagtacgat attggcttg atggatgag 
      aacagttat ttggagggg catgtgatt gacggccgc 
      aatttgttt ccagctact ggttatttg aacataatt 
      tgggagata atagccaaa gagcgctac gcgcaaccc 
      attcatttg cccatagtt ttcgaaaat tgcaaattt 
      gtaagagct accaccatg cccaaagct ggatttatc 
      acatttgcc gtgtcaatc cagcgtaaa agtgggttt 
      tttgaaatt gtcgaaaac gacagtatt gtcatgaca 
      ggacgtgtc ttggtacca gatgacatc aagtcgtac 
      caagtggat ttacccgaa ttaaaagtt agtgacgat 
      ccgaaggag tacttggac gagtttgaa gtttacaag 
      gagttgtat ttgcgtggt tacaattac agtgggctg 
      ttcaaaggc ttggtcaag tgtaatatt gatgggtca 
      tctgggttg gtccgatgg gacaataac tggatcagt 
      tttatggat aaaatgcta caaattaaa attttggaa 
      ctcgattcg aggctttta tatgttccg acaggaatc 
      aaaaaattg tcaattgat ccgatcaaa catctagaa 
      attgtcgaa aaaaaaagt gaccacttg atcccggtc 
      tcgtcatac aaaaacagc gatgttatc agatgtggc 
      gggatcgaa attttgggc ctccaggcc aacgccatc 
      tcgaaacgc aaaccaatc ctggaacca gtattggaa 
      aaaagtatt tttatcccc aacgcaact gatttggat 
      ttaactcaa gcaatccga gtcaacact cagataata 
      cttgacgaa agtttggaa aaaaatttt acggcattg 
      gaaattcta gacgagttt agcaatcct gaagtgtct 
      tttgtgatg ccacttttg tttaacgcc cttgaagac 
      attccctta attcaccca aaattgaca atttcaacc 
      aaaaccaaa attgacgac atcccagga gttaaaatc 
      gaagctacg actttatcg tcaactagc aatttatta 
      ttaattgtt gccacaaaa ttatttcaa cgtgtgact 
      ctcctgaac caagttttg tcagctttg agccaaaac 
      ggctttgct ctaagtcga gaggacgca aattttgag 
      cccgaattc ggcgataaa atcacaatt ctaacccaa 
      tttgtaaca cctgatgag aaattaatt ctgttcaaa 
      aaaacagaa agcggcaaa tttaatcaa aaactgatt 
      aacgtaagt tcggaaaat ttcaattgg gtccaacac 
      gttaaggaa gaactgaaa actgacaca cgtgtggtg 
      ctctacagt gaaaagggg gcccttgag gggctttta 
      gggctcgtt aactgtctc cgaaaggag ccaggcggc 
      accaaaatc tcatgtgtt tccatcatg ggacaagaa 
      ataccacaa ctggacgaa caattggcc aaaaatttg 
      gctttcaat gtttacaaa aaccaaaag tgggggact 
      taccgacat ttgcatttg gatgaggcg gtgcaaagt 
      cgcaaggac catgcgttt gttagttta ttgtcacgt 
      ggcgacttg tccagcttg cgatgggtg gaggggccg 
      ttgtccaag acggaccca aaggttatt tttgccaac 
      tactgcgcc atcaacttc aaggacatc atgacagca 
      accggccgc atcgtacta gccatggaa cgcaccaaa 
      caagactca atcgaaggc ctggagtac agtggacgc 
      gactttaac ggtaccaga atcatgggc atgacggac 
      catcgagcc atgggtact ttaatcaac ccagatttg 
      cccctttgt tggcggata cccgaccat tggtccctc 
      gaagacgca gccacggta ccagtggtt tacagtacc 
      gtcatttac ggccttttg atgcgctcg actttggca 
      cccggtact tcgattttg atccattct ggcaccggt 
      ggcattggc cttgcggcc ttgaatgtg gccttggca 
      catggttgt gacgtttac gtcacggtg ggcaccaaa 
      gaaaaacgc gagtttttg cgaaaaaac tttccacaa 
      atttcagat gaccacatc ggcaattct cgcgacttg 
      tcgttcgaa gaaatgatc aaaacgcaa accaaaggc 
      cgtggtgtt gacattgtt ttgaattct ttgacggag 
      gaaaagttg agggcctct gttaggtgt ctggcacgt 
      ggtggcaaa ttttttgaa attggcaaa ttcgattta 
      gttaacaat aatgcgatt caactttta ttgcttgag 
      aaaggtgcc agttatcac ggaattatg gttgatacg 
      ctctttaaa gagtcagat gaagacaaa ttgaaactt 
      aaccgaaaa gttgaagag gggataagg aagggttac 
      gttaaaccg atcccaagg actgtcttc gaaagtgac 
      aatgttgag gctgccttc cggtacatg atgaccgga 
      aaacacatc ggaaaggtt ttagtgaag attagggaa 
      gaaaatcaa gctgactgt tccattcag tgtttgcct 
      aaattctgg gctgatcca agcaaatcc tacataatc 
      ctaggtggc ttgggcggt tttggctta gaattagcc 
      gattggttg gtcttgaga ggagctcga aacctcgtc 
      ttaacttca agaactggc gtccaaacc ggttatcaa 
      acgcaacga atcaaaatc tggcaatca tacggcgct 
      cgcgtcaac atttccaca atcaccatt tcgtcaaaa 
      gacgattgc gtcaaattg atacaagaa gccacaaag 
      ttggctcca gttgacgca attttcaac ctcgctgtt 
      gtcctaaag gacgaatta ttcgaaaac caaaccgaa 
      gaaaatttc cggatttct ctcacaccg aaagctttc 
      gccaccaaa tatctggac caagtctcg agagatttg 
      tgtccgaat ttgcgattt ttcgtcgtg ttttcatcc 
      gtttcctgc ggacgtgga aacatagga cagagcaat 
      tatggcatg gccaactcg atcatggag cgagtttgc 
      gaaaaaaga aaaagtgaa gggtttcca gccttggcc 
      atccaatgg ggagcaatt ggagacgtc ggccttgtt 
      gccaaaatg caaaaagac aacaaagaa ctagtaatt 
      ggaggaact ctccaacaa aaaatctcc agctgtctt 
      aacgtctta gaccgatta ttaaaccaa gacgaacca 
      atcgtttcg tcaatggtt gtggcggaa aaacgcgat 
      aagagaatc ggtggtgaa agtgcagtt gacgtagtt 
      gccgctctt ttaggcatc aaagacatt aaaacagtc 
      agtcagcaa tcatctcta tccgaactc ggaatggac 
      tcaatgatg ggcaatgaa gtgatgcaa gtgctggaa 
      aaagaattc gaaatttat gtcacacct aaagatctc 
      agaaccatg acttttgcc aaattagtc gagctggag 
      gcgcaaaga acgtcaaat attcaagac caaaaaagc 
      aagcaaaaa atggaacag gggacaaat accattatc 
      cagtttatt ccagacgga caattcgat aatcaggag 
      ttaattaaa ttagagaga gttgaagga gtgtttaaa 
      cagttggaa ccttttgtc aaaaatttg aatgccagg 
      gtttttggg gtgcaatat tcgtaccaa caaccagaa 
      cacacgatt caagaagtt gtcgaaaat attcttcca 
      aaaatcgag aaacacatc aaaaccgaa aaaaaattc 
      ttcttcatc ggctactcg ttcggagtg acggtggcc 
      ttggagctg gccctccgc ctcgaagcc gcttcttac 
      accggcacc gtcgtggcc atcgacggc tccccgcac 
      tacaccagg cgcctgctc gagcactac ctcccgggg 
      gagaccgcc gccgagttc gagagcatg atcctggtg 
      aacatcctc gccatcttc ctccctctg gacctcatc 
      gtcaagaac aaggagaaa ctgatgagt tgtccggac 
      tttgacgcg cgcattgaa accgtgcgc agtttcgtg 
      ccttccgac ttgcccaac ttgcagaaa atggacaaa 
      caggtggcg ttcacgctc cgggagcga ttccgggcg 
      attttagac tacaagtca aacggcaac aagttgaaa 
      tctacgacg atcaagttg tacaaggcg gaacatccg 
      ccagtggcg ctcgaaaac atggacgag gattataag 
      ttggccgag tttagtgaa agtaaagtt gaggcgatg 
      acgatttgt gggaaccat atctcgatg ttggagcag 
      aaggagttg gtcaatgat gtgagccaa attttcgcg 
      ctggatttc tattaa
    mRNA sequence
    • >TC015399 RA
      ttccccaaa catggactc aaacaccac caacttcca 
      attcggaca atggctggc aactccacc gcctggaga 
      ggaagtcgt cgttacagg gatgtcggg gcgattccc 
      ggcctccaa caacgtcca ggaatttcg ggacaatct 
      cttcgccaa gaaatacat gatggacaa aatcgacaa 
      acgctggaa ggtcgtaaa caaggagat tccttcaaa 
      aggtggcct tcttcccga aatcgggac ttttgatcc 
      gggattttt cgggattca tcatcgaca agccgaaaa 
      catggaccc tgcgatgcg caatttcct ggaagttgc 
      tgtggaggc gattctgga ttcgggatt ccaccccaa 
      ggagttgga ggggagcag gacgggggt ttttgtggg 
      gtactcctg gtcggacct ggaggcgcc cagtttgag 
      cgaagccga cgagcccca aaagttcgg gatgacggg 
      ctacagccg cagtttcat tccacatcg catcagcta 
      ctacttgaa gctcaaagg gccttccgt tgtggtgga 
      ctcggcttg tagcagctc gttcaatgg ccttgagca 
      tgccttcaa ggccataag gacgggcaa gtgtgataa 
      tgcccttgt gggcggttg taacctaac ccttcatcc 
      tggtaccac cctacagtt tgcaaggta cggcctttt 
      gagcaaaga ttgcatttg caaagtgtt tgatcaaga 
      ggctaatgg atatgtgcg aagtgagac aattgcttg 
      tgtttttct gcaaaaggc aaaaaattc acgcaggat 
      ttatgccca aatcgtggg tgcaaaaat caacgacga 
      cggtttcaa aagcgaagg cctatcttt tccctctca 
      caaaatgca aggccaact cctcaaaga aatctacaa 
      cgaatgtgg aatagtacc atccagaat aacctacat 
      ggaactaca tggtactgg taccaaagt gggcgatgg 
      ggaagagct tcaagctgt agacgacgc cattgtgac 
      ccaacgtga aaaaaccct aattatagg ttccgtcaa 
      gtcaaacat aggccatgc ggaaccagc ttctggtat 
      gtcctccct tatcaaggt actgatagc tatggaaga 
      gggaaaaat tgcaccaaa catgaactt gaaccaaat 
      taaggccgg gttcacatc ccttgagca gggaaaatt 
      aagggtggc cacggaaat caccccctt ggaggggga 
      agaacccat tgttgggat tagcaattt tggattcgg 
      tggtaacaa ttgtcacgt gattttgaa acgcgtgtc 
      caaaaagaa cctgaagta ccatgacga agtaccacg 
      actggtttg tgtgagtgg tcgtactgt tcaagcagt 
      tgaagaaat acttaataa ggttaaatc aaaattcaa 
      cgttgaatt tatcgcaat gttgcacca actcttcaa 
      aaatcgctt taccacgca tttgtaccg tgggtacgc 
      cctagtgtc aaaaaatgg tacttttgg tacttcaat 
      tagccaaat aaagaacga atcaaacga gtgccgggt 
      gcatttcgg caactttca aaagtgtta ccttgaagt 
      ttgtcagta ctttatgtt attcccaaa atttcgaag 
      taccatgga acgtataaa tgaaatttt gggcggtgg 
      taccacctt atacgacac tctttcgag taccaaaac 
      caccctact tgcaagcgt gtgcacaca attctcaat 
      ccttgaaac tttgcgtga ccttggagt ggtaccaaa 
      actggacag taccaccaa cttggctca cttgatcca 
      aacattacc actggaaca gttactcga aacggtgcg 
      aaacattga ctcggaaaa ccacaaatt tcaagtttt 
      gactgcaag taccagaac ccaaatttt ggaacccga 
      acccgaaaa tgaccaaac ttttgtgtt ggacgtgac 
      aaaaggttt ggttgcgtt cttagacgg gcttggacg 
      gttgtacca aaatggagt tgatttgca tttagaaaa 
      gttgtatgc accggttga atttccggt cagtcgggc 
      cacgcccat gatatcgcc attggtcaa gtggtacca 
      tgacaagat ttggcacag tactgcttt tacagcatt 
      tgcgaaaaa aaccgacgc caagtacga tattggctt 
      gatggatga gaacagtta tttggaggg gcatgtgat 
      tgacggccg caatttgtt tccagctac tggttattt 
      gaacataat ttgggagat aatagccaa agagcgcta 
      cgcgcaacc cattcattt gcccatagt tttcgaaaa 
      ttgcaaatt tgtaagagc taccaccat gcccaaagc 
      tggatttat cacatttgc cgtgtcaat ccagcgtaa 
      aagtgggtt ttttgaaat tgtcgaaaa cgacagtat 
      tgtcatgac aggacgtgt cttggtacc agatgacat 
      caagtcgta ccaagtgga tttacccga attaaaagt 
      tagtgacga tccgaagga gtacttgga cgagtttga 
      agtttacaa ggagttgta tttgcgtgg ttacaatta 
      cagtgggct gttcaaagg cttggtcaa gtgtaatat 
      tgatgggtc atctgggtt ggtccgatg ggacaataa 
      ctggatcag ttttatgga taaaatgct acaaattaa 
      aattttgga actcgattc gaggctttt atatgttcc 
      gacaggaat caaaaaatt gtcaattga tccgatcaa 
      acatctaga aattgtcga aaaaaaaag tgaccactt 
      gatcccggt ctcgtcata caaaaacag cgatgttat 
      cagatgtgg cgggatcga aattttggg cctccaggc 
      caacgccat ctcgaaacg caaaccaat cctggaacc 
      agtattgga aaaaagtat ttttatccc caacgcaac 
      tgatttgga tttaactca agcaatccg agtcaacac 
      tcagataat acttgacga aagtttgga aaaaaattt 
      tacggcatt ggaaattct agacgagtt tagcaatcc 
      tgaagtgtc ttttgtgat gccactttt gtttaacgc 
      ccttgaaga cattccctt aattcaccc aaaattgac 
      aatttcaac caaaaccaa aattgacga catcccagg 
      agttaaaat cgaagctac gactttatc gtcaactag 
      caatttatt attaattgt tgccacaaa attatttca 
      acgtgtgac tctcctgaa ccaagtttt gtcagcttt 
      gagccaaaa cggctttgc tctaagtcg agaggacgc 
      aaattttga gcccgaatt cggcgataa aatcacaat 
      tctaaccca atttgtaac acctgatga gaaattaat 
      tctgttcaa aaaaacaga aagcggcaa atttaatca 
      aaaactgat taacgtaag ttcggaaaa tttcaattg 
      ggtccaaca cgttaagga agaactgaa aactgacac 
      acgtgtggt gctctacag tgaaaaggg ggcccttga 
      ggggctttt agggctcgt taactgtct ccgaaagga 
      gccaggcgg caccaaaat ctcatgtgt ttccatcat 
      gggacaaga aataccaca actggacga acaattggc 
      caaaaattt ggctttcaa tgtttacaa aaaccaaaa 
      gtgggggac ttaccgaca tttgcattt ggatgaggc 
      ggtgcaaag tcgcaagga ccatgcgtt tgttagttt 
      attgtcacg tggcgactt gtccagctt gcgatgggt 
      ggaggggcc gttgtccaa gacggaccc aaaggttat 
      ttttgccaa ctactgcgc catcaactt caaggacat 
      catgacagc aaccggccg catcgtact agccatgga 
      acgcaccaa acaagactc aatcgaagg cctggagta 
      cagtggacg cgactttaa cggtaccag aatcatggg 
      catgacgga ccatcgagc catgggtac tttaatcaa 
      cccagattt gcccctttg ttggcggat acccgacca 
      ttggtccct cgaagacgc agccacggt accagtggt 
      ttacagtac cgtcattta cggcctttt gatgcgctc 
      gactttggc acccggtac ttcgatttt gatccattc 
      tggcaccgg tggcattgg ccttgcggc cttgaatgt 
      ggccttggc acatggttg tgacgttta cgtcacggt 
      gggcaccaa agaaaaacg cgagttttt gcgaaaaaa 
      ctttccaca aatttcaga tgaccacat cggcaattc 
      tcgcgactt gtcgttcga agaaatgat caaaacgca 
      aaccaaagg ccgtggtgt tgacattgt tttgaattc 
      tttgacgga ggaaaagtt gagggcctc tgttaggtg 
      tctggcacg tggtggcaa attttttga aattggcaa 
      attcgattt agttaacaa taatgcgat tcaactttt 
      attgcttga gaaaggtgc cagttatca cggaattat 
      ggttgatac gctctttaa agagtcaga tgaagacaa 
      attgaaact taaccgaaa agttgaaga ggggataag 
      gaagggtta cgttaaacc gatcccaag gactgtctt 
      cgaaagtga caatgttga ggctgcctt ccggtacat 
      gatgaccgg aaaacacat cggaaaggt tttagtgaa 
      gattaggga agaaaatca agctgactg ttccattca 
      gtgtttgcc taaattctg ggctgatcc aagcaaatc 
      ctacataat cctaggtgg cttgggcgg ttttggctt 
      agaattagc cgattggtt ggtcttgag aggagctcg 
      aaacctcgt cttaacttc aagaactgg cgtccaaac 
      cggttatca aacgcaacg aatcaaaat ctggcaatc 
      atacggcgc tcgcgtcaa catttccac aatcaccat 
      ttcgtcaaa agacgattg cgtcaaatt gatacaaga 
      agccacaaa gttggctcc agttgacgc aattttcaa 
      cctcgctgt tgtcctaaa ggacgaatt attcgaaaa 
      ccaaaccga agaaaattt ccggatttc tctcacacc 
      gaaagcttt cgccaccaa atatctgga ccaagtctc 
      gagagattt gtgtccgaa tttgcgatt tttcgtcgt 
      gttttcatc cgtttcctg cggacgtgg aaacatagg 
      acagagcaa ttatggcat ggccaactc gatcatgga 
      gcgagtttg cgaaaaaag aaaaagtga agggtttcc 
      agccttggc catccaatg gggagcaat tggagacgt 
      cggccttgt tgccaaaat gcaaaaaga caacaaaga 
      actagtaat tggaggaac tctccaaca aaaaatctc 
      cagctgtct taacgtctt agaccgatt attaaacca 
      agacgaacc aatcgtttc gtcaatggt tgtggcgga 
      aaaacgcga taagagaat cggtggtga aagtgcagt 
      tgacgtagt tgccgctct tttaggcat caaagacat 
      taaaacagt cagtcagca atcatctct atccgaact 
      cggaatgga ctcaatgat gggcaatga agtgatgca 
      agtgctgga aaaagaatt cgaaattta tgtcacacc 
      taaagatct cagaaccat gacttttgc caaattagt 
      cgagctgga ggcgcaaag aacgtcaaa tattcaaga 
      ccaaaaaag caagcaaaa aatggaaca ggggacaaa 
      taccattat ccagtttat tccagacgg acaattcga 
      taatcagga gttaattaa attagagag agttgaagg 
      agtgtttaa acagttgga accttttgt caaaaattt 
      gaatgccag ggtttttgg ggtgcaata ttcgtacca 
      acaaccaga acacacgat tcaagaagt tgtcgaaaa 
      tattcttcc aaaaatcga gaaacacat caaaaccga 
      aaaaaaatt cttcttcat cggctactc gttcggagt 
      gacggtggc cttggagct ggccctccg cctcgaagc 
      cgcttctta caccggcac cgtcgtggc catcgacgg 
      ctccccgca ctacaccag gcgcctgct cgagcacta 
      cctcccggg ggagaccgc cgccgagtt cgagagcat 
      gatcctggt gaacatcct cgccatctt cctccctct 
      ggacctcat cgtcaagaa caaggagaa actgatgag 
      ttgtccgga ctttgacgc gcgcattga aaccgtgcg 
      cagtttcgt gccttccga cttgcccaa cttgcagaa 
      aatggacaa acaggtggc gttcacgct ccgggagcg 
      attccgggc gattttaga ctacaagtc aaacggcaa 
      caagttgaa atctacgac gatcaagtt gtacaaggc 
      ggaacatcc gccagtggc gctcgaaaa catggacga 
      ggattataa gttggccga gtttagtga aagtaaagt 
      tgaggcgat gacgatttg tgggaacca tatctcgat 
      gttggagca gaaggagtt ggtcaatga tgtgagcca 
      aattttcgc gctggattt ctattaaag tgggacact 
      gatgtggca aaatagagg tgtcagttt agtgatcgt 
      ttaataaat ttttaactt ttga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY