| General information | |||
| TC015393 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 251154..255231 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015393 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05809 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC015393 RA
MLLLIVFSL ILSVISDPN VPPFCDKCA CVNDDSNQF VVTCTKDVS SFMFDKASW IDAADNKPY SYSKVWIQN SPILTLKEQ FPVSNLIEL SLANNSIVS ISSDVFSNL QNMQMLDLS YNNIEILHP DAFNGQYLE GDWNPLRSL VKLYLAHNR IHSLDKDIF EHADRIELL SLSHNPLKI LDQSTAIAI TSLVYLKNL DLSYTGLSS LPAAFLHTP KKLIFLDLS GNQFTGIPD TLGDAHSLE TLYFNNNPM VNLTNKNGF PVISTLKYL HLSSMPNLV NISKESLSN LVNLTEFYC FNNPKLSYI DRDAFSSRR DGAEYKTWP PIKKLFLQN NKLASMDME VVLNWKDLV ALDLTENAW TCECENQWM IDELMPIYL QLDENKAKA LKCEAPIEM ASLTFYDVY QKQTTMRCL DLYGHRPER DGVLLVGIL AGVLITIPL VLFIIYAYQ RHWFGLFDN SPAGFSRQF YKRSVSTSE DAYL
- >TC015393 RA
atgttgtta cttattgta ttctcactg attttatcg gtgatttcg gacccaaac gttccaccc ttttgcgac aaatgtgcg tgtgttaat gacgattcc aatcaattt gtggtcact tgtaccaaa gacgtttcg tcctttatg tttgataaa gcatcatgg atcgatgca gctgataac aagccgtac agttattcc aaagtttgg attcagaat tcaccgatt ctgacgtta aaagagcaa tttccagtc tcaaaccta atcgagttg agtttagcc aacaattcg attgttagc attagcagt gatgtgttt agcaacctc caaaacatg caaatgttg gacctgagt tacaacaac attgaaatt ttgcatcct gatgctttt aatggccaa tatttggaa ggtgactgg aatccttta cgatccttg gttaagctc tatttggct cataacagg attcatagt cttgataag gatattttc gagcatgct gacaggatt gaactttta tcactgtct cacaatcct ttaaaaatc ctagatcag tcaactgca atagcaatc actagtttg gtctatttg aaaaatttg gatttgagt tatacaggt ttaagcagt cttccggct gcttttttg cacactcca aaaaaatta attttcttg gatttgagc ggaaaccag tttacggga attcccgat actttaggg gatgcacac agcttagaa acgctttat tttaataat aatcccatg gttaatttg accaataaa aacggtttt cctgtcatt tccactttg aaatatctc cacttgagt tcgatgccc aacttggtc aatatcagt aaagaatct ttaagtaat cttgttaat ttaactgaa ttttattgc ttcaacaac cccaaatta tcgtacatt gaccgagac gcgttttcg tcacgacgt gacggtgcc gaatacaaa acttggcca ccgatcaaa aaacttttt ctacaaaat aataaatta gcttcgatg gatatggaa gttgttttg aattggaag gatttggtt gcgcttgat ttaacagaa aacgcgtgg acgtgtgag tgcgaaaat caatggatg attgacgaa ttgatgccc atttatttg caactggac gagaataag gccaaagca cttaagtgt gaggcccca atcgagatg gcttccctg acattctac gacgtttat cagaaacaa acaacgatg cgatgtctt gacttatac ggtcataga cccgaacga gacggcgtt ctattggtt ggaattttg gctggtgtt ttaatcaca ataccattg gtattgttc ataatttac gcttatcag agacactgg tttggatta tttgataat tcaccggct ggtttttca cgccagttt tacaaacga agcgtttcg acatcagaa gacgcttat ctttaa
- >TC015393 RA
gtgtaatgc aggaaatgc aaattttca gtatatttg ttgacgtat ttgttttcc atttataaa gcgaacgaa aggttaagt gtgacgtaa attaaagaa aatgcaatt ttagaattt acaaagctt tccagttgt tcaaacctg aagagtcta cgaccaact ggtccaaag ttattaatg tgccattcg ggtgcaaaa atcattttc tggatctgt gaattacta ggtaatttt tcacttgac ttggtaatt aattatcaa aaaacaatc atttttatg gcatctggt agagaattt cctgggctt tccagtggt ctaaacgcg aaaagtcta cgattatct ggaccaaag ttattgcta aaattcctg tcttatgta gaaatttat ttttagatc aatgagtca ctacgcaat ttttccctg actcggcaa ttaaacatc ataaaacaa tgactttta cttcacttg atagacaat ttcccaagc tttccaatg gtataaact gcaagactc tacaattac gtggtccaa atttattgc cattttcca agcttaaaa atctgtttt tggttaata cgttgctaa gcaattttt tgcctgact cggctacta gacatcaca aagcaatga gttttactt catttgata gagaatttt ccgggcttt ccattggta tagacggtg aaaacgtgc gaccatgtg ggtcaaact tattccttc catgtgtta attattttt tggacaaat gaatcgcta agtaatttt tttgtttta ctcggctac tagatatcc taaaacaat ggtttttac ttcattgga ttgagaatt tttcgggct ttccaatgg tacaaacgg caaaaacgt gcgaccaag taggctaaa gttattgcc aaattaatt tcgggtgta ttggatcaa tgagtcact cttgtatta accagaacg cagttatca cttatttgt ttaatatac ctgacgaaa ttttgttta aacaatgat aagataagt gagaattat tctgtcaac aacgaattg aaacattca gtgcgacat tattttgta attgtttta gtagtgagc caaaattag tgtaaaatc aagtgccaa aattgcagt ttttcgtcc agaaatcac caatgttgt tacttattg tattctcac tgattttat cggtgattt cggacccaa acgttccac ccttttgcg acaaatgtg cgtgtgtta atgacgatt ccaatcaat ttgtggtca cttgtacca aagacgttt cgtccttta tgtttgata aagcatcat ggatcgatg cagctgata acaagccgt acagttatt ccaaagttt ggattcaga attcaccga ttctgacgt taaaagagc aatttccag tctcaaacc taatcgagt tgagtttag ccaacaatt cgattgtta gcattagca gtgatgtgt ttagcaacc tccaaaaca tgcaaatgt tggacctga gttacaaca acattgaaa ttttgcatc ctgatgctt ttaatggcc aatatttgg aaggtgact ggaatcctt tacgatcct tggttaagc tctatttgg ctcataaca ggattcata gtcttgata aggatattt tcgagcatg ctgacagga ttgaacttt tatcactgt ctcacaatc ctttaaaaa tcctagatc agtcaactg caatagcaa tcactagtt tggtctatt tgaaaaatt tggatttga gttatacag gtttaagca gtcttccgg ctgcttttt tgcacactc caaaaaaat taattttct tggatttga gcggaaacc agtttacgg gaattcccg atactttag gggatgcac acagcttag aaacgcttt attttaata ataatccca tggttaatt tgaccaata aaaacggtt ttcctgtca tttccactt tgaaatatc tccacttga gttcgatgc ccaacttgg tcaatatca gtaaagaat ctttaagta atcttgtta atttaactg aattttatt gcttcaaca accccaaat tatcgtaca ttgaccgag acgcgtttt cgtcacgac gtgacggtg ccgaataca aaacttggc caccgatca aaaaacttt ttctacaaa ataataaat tagcttcga tggatatgg aagttgttt tgaattgga aggatttgg ttgcgcttg atttaacag aaaacgcgt ggacgtgtg agtgcgaaa atcaatgga tgattgacg aattgatgc ccatttatt tgcaactgg acgagaata aggccaaag cacttaagt gtgaggccc caatcgaga tggcttccc tgacattct acgacgttt atcagaaac aaacaacga tgcgatgtc ttgacttat acggtcata gacccgaac gagacggcg ttctattgg ttggaattt tggctggtg ttttaatca caataccat tggtattgt tcataattt acgcttatc agagacact ggtttggat tatttgata attcaccgg ctggttttt cacgccagt tttacaaac gaagcgttt cgacatcag aagacgctt atctttaat tatttgttt agttttttt ttaataaaa ttaaattaa attt
